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NCK2 NCK adaptor protein 2 [ Homo sapiens (human) ]

Gene ID: 8440, updated on 27-Nov-2024

Summary

Official Symbol
NCK2provided by HGNC
Official Full Name
NCK adaptor protein 2provided by HGNC
Primary source
HGNC:HGNC:7665
See related
Ensembl:ENSG00000071051 MIM:604930; AllianceGenome:HGNC:7665
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
GRB4; NCKbeta
Summary
This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in duodenum (RPKM 14.7), spleen (RPKM 14.6) and 25 other tissues See more
Orthologs
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Try the new Transcript table

Genomic context

See NCK2 in Genome Data Viewer
Location:
2q12.2
Exon count:
13
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 2 NC_000002.12 (105744453..105894272)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 2 NC_060926.1 (106205667..106355582)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (106361369..106510728)

Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene long intergenic non-protein coding RNA 2946 Neighboring gene uncharacterized LOC107985929 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16317 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_52468 Neighboring gene ReSE screen-validated silencer GRCh37_chr2:106315285-106315464 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11840 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11841 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11842 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16318 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16319 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16320 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:106378480-106379325 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:106385690-106386192 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:106386193-106386695 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16321 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16322 Neighboring gene H3K27ac hESC enhancer GRCh37_chr2:106394362-106394862 Neighboring gene H3K27ac hESC enhancer GRCh37_chr2:106399357-106399857 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:106403143-106403714 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16326 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16325 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16324 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11843 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11844 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16327 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16328 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16329 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16330 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16331 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:106428458-106428958 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:106428959-106429459 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16332 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16333 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16334 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16335 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:106497364-106498144 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:106498145-106498923 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16336 Neighboring gene uncharacterized LOC105373530 Neighboring gene uncharacterized LOC105373531 Neighboring gene NANOG hESC enhancer GRCh37_chr2:106567089-106567923 Neighboring gene uncharacterized LOC124906055

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phenotypes

EBI GWAS Catalog

Description
Genome-wide meta-analysis of systolic blood pressure in children with sickle cell disease.
EBI GWAS Catalog
NCK2 is significantly associated with opiates addiction in African-origin men.
EBI GWAS Catalog

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120 downregulates the expression of NCK adaptor protein 2 (NCK2) in human B cells PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables cytoskeletal anchor activity NAS
Non-traceable Author Statement
more info
PubMed 
enables phosphotyrosine residue binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein-containing complex binding IEA
Inferred from Electronic Annotation
more info
 
enables receptor tyrosine kinase binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables scaffold protein binding IEA
Inferred from Electronic Annotation
more info
 
enables signaling adaptor activity IBA
Inferred from Biological aspect of Ancestor
more info
 
enables signaling adaptor activity NAS
Non-traceable Author Statement
more info
PubMed 
enables signaling receptor complex adaptor activity NAS
Non-traceable Author Statement
more info
PubMed 
Process Evidence Code Pubs
involved_in T cell activation NAS
Non-traceable Author Statement
more info
PubMed 
involved_in actin filament organization IEA
Inferred from Electronic Annotation
more info
 
involved_in cell migration IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in dendritic spine development IEA
Inferred from Electronic Annotation
more info
 
involved_in ephrin receptor signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in epidermal growth factor receptor signaling pathway TAS
Traceable Author Statement
more info
PubMed 
involved_in immunological synapse formation IEA
Inferred from Electronic Annotation
more info
 
involved_in lamellipodium assembly IEA
Inferred from Electronic Annotation
more info
 
involved_in negative regulation of PERK-mediated unfolded protein response IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in negative regulation of cell population proliferation TAS
Traceable Author Statement
more info
PubMed 
involved_in negative regulation of transcription by RNA polymerase II IEA
Inferred from Electronic Annotation
more info
 
involved_in positive regulation of T cell proliferation IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in positive regulation of actin filament polymerization IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of translation in response to endoplasmic reticulum stress IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in signal complex assembly NAS
Non-traceable Author Statement
more info
PubMed 
involved_in signal transduction TAS
Traceable Author Statement
more info
PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytoplasm NAS
Non-traceable Author Statement
more info
PubMed 
located_in cytosol TAS
Traceable Author Statement
more info
 
located_in endoplasmic reticulum IDA
Inferred from Direct Assay
more info
PubMed 
located_in postsynaptic density IEA
Inferred from Electronic Annotation
more info
 
located_in vesicle membrane IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
cytoplasmic protein NCK2
Names
SH2/SH3 adaptor protein NCK-beta
growth factor receptor-bound protein 4
noncatalytic region of tyrosine kinase, beta

NCBI Reference Sequences (RefSeq)

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001004720.3NP_001004720.1  cytoplasmic protein NCK2 isoform A

    See identical proteins and their annotated locations for NP_001004720.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (A).
    Source sequence(s)
    AC009505, BC000103, DB456958
    Consensus CDS
    CCDS33266.1
    UniProtKB/Swiss-Prot
    D3DVK1, O43639, Q9BWN9, Q9UIC3
    UniProtKB/TrEMBL
    A0A0S2Z4M6, Q53TG4
    Related
    ENSP00000377018.2, ENST00000393349.2
    Conserved Domains (4) summary
    cd10409
    Location:283380
    SH2_Nck2; Src homology 2 (SH2) domain found in Nck
    cd11899
    Location:259
    SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein
    cd11902
    Location:114168
    SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein
    cd11903
    Location:198256
    SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein
  2. NM_001004722.4NP_001004722.1  cytoplasmic protein NCK2 isoform B

    See identical proteins and their annotated locations for NP_001004722.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks an exon, which results in a frameshift, compared to variant 1. The resulting protein (isoform B) is shorter and has a distinct C-terminus, compared to isoform A.
    Source sequence(s)
    AC009505, AC010978, BG480643
    UniProtKB/TrEMBL
    E7ERP6
    Related
    ENSP00000410428.2, ENST00000451463.6
    Conserved Domains (1) summary
    cl17036
    Location:259
    SH3; Src Homology 3 domain superfamily
  3. NM_003581.5NP_003572.2  cytoplasmic protein NCK2 isoform A

    See identical proteins and their annotated locations for NP_003572.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (A). Variants 1 and 2 both encode isoform A.
    Source sequence(s)
    AC009505, AC010978, BC007195
    Consensus CDS
    CCDS33266.1
    UniProtKB/Swiss-Prot
    D3DVK1, O43639, Q9BWN9, Q9UIC3
    UniProtKB/TrEMBL
    A0A0S2Z4M6, Q53TG4
    Related
    ENSP00000233154.4, ENST00000233154.9
    Conserved Domains (4) summary
    cd10409
    Location:283380
    SH2_Nck2; Src homology 2 (SH2) domain found in Nck
    cd11899
    Location:259
    SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein
    cd11902
    Location:114168
    SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein
    cd11903
    Location:198256
    SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000002.12 Reference GRCh38.p14 Primary Assembly

    Range
    105744453..105894272
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017005105.2XP_016860594.1  cytoplasmic protein NCK2 isoform X1

    UniProtKB/Swiss-Prot
    D3DVK1, O43639, Q9BWN9, Q9UIC3
    UniProtKB/TrEMBL
    A0A0S2Z4M6, Q53TG4
    Conserved Domains (4) summary
    cd10409
    Location:283380
    SH2_Nck2; Src homology 2 (SH2) domain found in Nck
    cd11899
    Location:259
    SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein
    cd11902
    Location:114168
    SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein
    cd11903
    Location:198256
    SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein
  2. XM_017005103.2XP_016860592.1  cytoplasmic protein NCK2 isoform X1

    UniProtKB/Swiss-Prot
    D3DVK1, O43639, Q9BWN9, Q9UIC3
    UniProtKB/TrEMBL
    A0A0S2Z4M6, Q53TG4
    Conserved Domains (4) summary
    cd10409
    Location:283380
    SH2_Nck2; Src homology 2 (SH2) domain found in Nck
    cd11899
    Location:259
    SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein
    cd11902
    Location:114168
    SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein
    cd11903
    Location:198256
    SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein
  3. XM_011511991.4XP_011510293.1  cytoplasmic protein NCK2 isoform X1

    See identical proteins and their annotated locations for XP_011510293.1

    UniProtKB/Swiss-Prot
    D3DVK1, O43639, Q9BWN9, Q9UIC3
    UniProtKB/TrEMBL
    A0A0S2Z4M6, Q53TG4
    Conserved Domains (4) summary
    cd10409
    Location:283380
    SH2_Nck2; Src homology 2 (SH2) domain found in Nck
    cd11899
    Location:259
    SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein
    cd11902
    Location:114168
    SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein
    cd11903
    Location:198256
    SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein
  4. XM_047446021.1XP_047301977.1  cytoplasmic protein NCK2 isoform X2

    UniProtKB/TrEMBL
    E7ERP6
    Related
    ENSP00000431109.1, ENST00000522586.5
  5. XM_006712797.4XP_006712860.1  cytoplasmic protein NCK2 isoform X1

    See identical proteins and their annotated locations for XP_006712860.1

    UniProtKB/Swiss-Prot
    D3DVK1, O43639, Q9BWN9, Q9UIC3
    UniProtKB/TrEMBL
    A0A0S2Z4M6, Q53TG4
    Conserved Domains (4) summary
    cd10409
    Location:283380
    SH2_Nck2; Src homology 2 (SH2) domain found in Nck
    cd11899
    Location:259
    SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein
    cd11902
    Location:114168
    SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein
    cd11903
    Location:198256
    SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein
  6. XM_047446019.1XP_047301975.1  cytoplasmic protein NCK2 isoform X1

    UniProtKB/Swiss-Prot
    D3DVK1, O43639, Q9BWN9, Q9UIC3
    UniProtKB/TrEMBL
    A0A0S2Z4M6
  7. XM_017005104.2XP_016860593.1  cytoplasmic protein NCK2 isoform X1

    UniProtKB/Swiss-Prot
    D3DVK1, O43639, Q9BWN9, Q9UIC3
    UniProtKB/TrEMBL
    A0A0S2Z4M6, Q53TG4
    Conserved Domains (4) summary
    cd10409
    Location:283380
    SH2_Nck2; Src homology 2 (SH2) domain found in Nck
    cd11899
    Location:259
    SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein
    cd11902
    Location:114168
    SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein
    cd11903
    Location:198256
    SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein
  8. XM_047446020.1XP_047301976.1  cytoplasmic protein NCK2 isoform X1

    UniProtKB/Swiss-Prot
    D3DVK1, O43639, Q9BWN9, Q9UIC3
    UniProtKB/TrEMBL
    A0A0S2Z4M6
  9. XM_047446017.1XP_047301973.1  cytoplasmic protein NCK2 isoform X1

    UniProtKB/Swiss-Prot
    D3DVK1, O43639, Q9BWN9, Q9UIC3
    UniProtKB/TrEMBL
    A0A0S2Z4M6
  10. XM_047446018.1XP_047301974.1  cytoplasmic protein NCK2 isoform X1

    UniProtKB/Swiss-Prot
    D3DVK1, O43639, Q9BWN9, Q9UIC3
    UniProtKB/TrEMBL
    A0A0S2Z4M6
  11. XM_011511992.3XP_011510294.2  cytoplasmic protein NCK2 isoform X2

    UniProtKB/TrEMBL
    E7ERP6
    Conserved Domains (1) summary
    cl17036
    Location:259
    SH3; Src Homology 3 domain superfamily

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060926.1 Alternate T2T-CHM13v2.0

    Range
    106205667..106355582
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054344191.1XP_054200166.1  cytoplasmic protein NCK2 isoform X1

  2. XM_054344188.1XP_054200163.1  cytoplasmic protein NCK2 isoform X1

  3. XM_054344192.1XP_054200167.1  cytoplasmic protein NCK2 isoform X1

  4. XM_054344196.1XP_054200171.1  cytoplasmic protein NCK2 isoform X2

  5. XM_054344193.1XP_054200168.1  cytoplasmic protein NCK2 isoform X1

  6. XM_054344190.1XP_054200165.1  cytoplasmic protein NCK2 isoform X1

  7. XM_054344189.1XP_054200164.1  cytoplasmic protein NCK2 isoform X1

  8. XM_054344194.1XP_054200169.1  cytoplasmic protein NCK2 isoform X1

  9. XM_054344195.1XP_054200170.1  cytoplasmic protein NCK2 isoform X2