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CDC45 cell division cycle 45 [ Homo sapiens (human) ]

Gene ID: 8318, updated on 8-Dec-2018

Summary

Official Symbol
CDC45provided by HGNC
Official Full Name
cell division cycle 45provided by HGNC
Primary source
HGNC:HGNC:1739
See related
Ensembl:ENSG00000093009 MIM:603465
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
CDC45L; MGORS7; CDC45L2; PORC-PI-1
Summary
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Expression
Biased expression in testis (RPKM 11.5), bone marrow (RPKM 6.8) and 11 other tissues See more
Orthologs

Genomic context

See CDC45 in Genome Data Viewer
Location:
22q11.21
Exon count:
21
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 22 NC_000022.11 (19479294..19520612)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 22 NC_000022.10 (19466984..19508135)

Chromosome 22 - NC_000022.11Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC105372860 Neighboring gene chromosome 22 open reading frame 39 Neighboring gene ubiquitin recognition factor in ER associated degradation 1 Neighboring gene claudin 5 Neighboring gene long intergenic non-protein coding RNA 895

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Jun 15 11:32:44 2016

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Pathways from BioSystems

  • Activation of ATR in response to replication stress, organism-specific biosystem (from REACTOME)
    Activation of ATR in response to replication stress, organism-specific biosystemGenotoxic stress caused by DNA damage or stalled replication forks can lead to genomic instability. To guard against such instability, genotoxically-stressed cells activate checkpoint factors that ha...
  • Activation of the pre-replicative complex, organism-specific biosystem (from REACTOME)
    Activation of the pre-replicative complex, organism-specific biosystemIn S. cerevisiae, two ORC subunits, Orc1 and Orc5, both bind ATP, and Orc1 in addition has ATPase activity. Both ATP binding and ATP hydrolysis appear to be essential functions in vivo. ATP binding b...
  • Cell Cycle, organism-specific biosystem (from WikiPathways)
    Cell Cycle, organism-specific biosystemThe cell cycle is the series of events that takes place in a cell leading to its division and duplication (replication). Regulation of the cell cycle involves processes crucial to the survival of a c...
  • Cell Cycle, organism-specific biosystem (from REACTOME)
    Cell Cycle, organism-specific biosystem
    Cell Cycle
  • Cell Cycle Checkpoints, organism-specific biosystem (from REACTOME)
    Cell Cycle Checkpoints, organism-specific biosystemA hallmark of the human cell cycle in normal somatic cells is its precision. This remarkable fidelity is achieved by a number of signal transduction pathways, known as checkpoints, which monitor cell...
  • Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
    Cell Cycle, Mitotic, organism-specific biosystemThe replication of the genome and the subsequent segregation of chromosomes into daughter cells are controlled by a series of events collectively known as the cell cycle. DNA replication is carried o...
  • Cell cycle, organism-specific biosystem (from KEGG)
    Cell cycle, organism-specific biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cycli...
  • Cell cycle, conserved biosystem (from KEGG)
    Cell cycle, conserved biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cycli...
  • DNA Replication, organism-specific biosystem (from REACTOME)
    DNA Replication, organism-specific biosystemStudies in the past decade have suggested that the basic mechanism of DNA replication initiation is conserved in all kingdoms of life. Initiation in unicellular eukaryotes, in particular Saccharomyce...
  • DNA Replication, organism-specific biosystem (from WikiPathways)
    DNA Replication, organism-specific biosystemStudies in the past decade have suggested that the basic mechanism of DNA replication initiation is conserved in all kingdoms of life. Initiation in unicellular eukaryotes, in particular Saccharomyce...
  • DNA Replication Pre-Initiation, organism-specific biosystem (from REACTOME)
    DNA Replication Pre-Initiation, organism-specific biosystemAlthough, DNA replication occurs in the S phase of the cell cycle, the formation of the DNA replication pre-initiation complex begins during G1 phase.
  • DNA strand elongation, organism-specific biosystem (from REACTOME)
    DNA strand elongation, organism-specific biosystemAccurate and efficient genome duplication requires coordinated processes to replicate two template strands at eucaryotic replication forks. Knowledge of the fundamental reactions involved in replicat...
  • E2F mediated regulation of DNA replication, organism-specific biosystem (from REACTOME)
    E2F mediated regulation of DNA replication, organism-specific biosystemProgression through G1 and G1 to S-phase transition that initiates DNA synthesis involve many complexes that are regulated by RB1:E2F pathway. RB1:E2F pathway plays a key role in gene expression regu...
  • G1 to S cell cycle control, organism-specific biosystem (from WikiPathways)
    G1 to S cell cycle control, organism-specific biosystemIn the G1 phase there are two types of DNA damage responses, the p53-dependent and the p53-independent pathways. The p53-dependent responses inhibit CDKs through the up-regulation of genes encoding C...
  • G1/S Transition, organism-specific biosystem (from REACTOME)
    G1/S Transition, organism-specific biosystemCyclin E - Cdk2 complexes control the transition from G1 into S-phase. In this case, the binding of p21Cip1/Waf1 or p27kip1 is inhibitory. Important substrates for Cyclin E - Cdk2 complexes include p...
  • G1/S-Specific Transcription, organism-specific biosystem (from REACTOME)
    G1/S-Specific Transcription, organism-specific biosystemThe E2F family of transcription factors regulate the transition from the G1 to the S phase in the cell cycle. E2F activity is regulated by members of the retinoblastoma protein (pRb) family, resultin...
  • G2/M Checkpoints, organism-specific biosystem (from REACTOME)
    G2/M Checkpoints, organism-specific biosystemG2/M checkpoints include the checks for damaged DNA, unreplicated DNA, and checks that ensure that the genome is replicated once and only once per cell cycle. If cells pass these checkpoints, they f...
  • M/G1 Transition, organism-specific biosystem (from REACTOME)
    M/G1 Transition, organism-specific biosystemFinally, progression out of mitosis and division of the cell into two daughters (cytokinesis) requires the inactivation of Cyclin B - Cdc2 by ubiquitin-dependent proteolysis of Cyclin A and B, which ...
  • Mitotic G1-G1/S phases, organism-specific biosystem (from REACTOME)
    Mitotic G1-G1/S phases, organism-specific biosystem
    Mitotic G1-G1/S phases
  • Retinoblastoma (RB) in Cancer, organism-specific biosystem (from WikiPathways)
    Retinoblastoma (RB) in Cancer, organism-specific biosystemDescribes the role of retinoblastoma (RB) gene in cancer.
  • S Phase, organism-specific biosystem (from REACTOME)
    S Phase, organism-specific biosystemDNA synthesis occurs in the S phase, or the synthesis phase, of the cell cycle. The cell duplicates its hereditary material, and two copies of the chromosome are formed. As DNA replication continues,...
  • Synthesis of DNA, organism-specific biosystem (from REACTOME)
    Synthesis of DNA, organism-specific biosystemThe actual synthesis of DNA occurs in the S phase of the cell cycle. This includes the initiation of DNA replication, when the first nucleotide of the new strand is laid down during the synthesis of ...
  • Unwinding of DNA, organism-specific biosystem (from REACTOME)
    Unwinding of DNA, organism-specific biosystemDNA Replication is regulated accurately and precisely by various protein complexes. Many members of the MCM protein family are assembled into the pre-Replication Complexes (pre-RC) at the end of M ph...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
3'-5' DNA helicase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
DNA replication origin binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
chromatin binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
single-stranded DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Process Evidence Code Pubs
DNA duplex unwinding IEA
Inferred from Electronic Annotation
more info
 
DNA replication TAS
Traceable Author Statement
more info
 
DNA replication checkpoint TAS
Traceable Author Statement
more info
PubMed 
DNA replication initiation IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
G1/S transition of mitotic cell cycle TAS
Traceable Author Statement
more info
 
double-strand break repair via break-induced replication IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
mitotic DNA replication preinitiation complex assembly IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
positive regulation of G1/S transition of mitotic cell cycle IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
regulation of chromatin silencing at telomere IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
regulation of transcription involved in G1/S transition of mitotic cell cycle TAS
Traceable Author Statement
more info
 
Component Evidence Code Pubs
DNA replication preinitiation complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
centrosome IDA
Inferred from Direct Assay
more info
 
cytoplasm IEA
Inferred from Electronic Annotation
more info
 
nuclear pre-replicative complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
replication fork protection complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 

General protein information

Preferred Names
cell division control protein 45 homolog
Names
CDC45 cell division cycle 45 homolog
CDC45 cell division cycle 45-like
CDC45-related protein
cell division cycle 45 homolog
cell division cycle 45-like 2
human CDC45

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_052958.1 RefSeqGene

    Range
    5001..45787
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178010.2NP_001171481.1  cell division control protein 45 homolog isoform 1

    See identical proteins and their annotated locations for NP_001171481.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1).
    Source sequence(s)
    AC000088, AF081535, AK293338, HY016491
    Consensus CDS
    CCDS54499.1
    UniProtKB/Swiss-Prot
    O75419
    Related
    ENSP00000405726.2, ENST00000437685.6
    Conserved Domains (1) summary
    pfam02724
    Location:19593
    CDC45; CDC45-like protein
  2. NM_001178011.2NP_001171482.1  cell division control protein 45 homolog isoform 3

    See identical proteins and their annotated locations for NP_001171482.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks two in-frame exons in the CDS, compared to variant 1. The resulting isoform (3) is shorter than isoform 1.
    Source sequence(s)
    AC000087, AF081535, AK293123, HY016491
    Consensus CDS
    CCDS54500.1
    UniProtKB/Swiss-Prot
    O75419
    Related
    ENSP00000384978.3, ENST00000404724.7
    Conserved Domains (1) summary
    pfam02724
    Location:19515
    CDC45; CDC45-like protein
  3. NM_003504.4NP_003495.1  cell division control protein 45 homolog isoform 2

    See identical proteins and their annotated locations for NP_003495.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an in-frame exon in the CDS, as compared to variant 1. The resulting isoform (2) is shorter than isoform 1.
    Source sequence(s)
    AF081535, BC006232, HY016491
    Consensus CDS
    CCDS13762.1
    UniProtKB/Swiss-Prot
    O75419
    Related
    ENSP00000263201.1, ENST00000263201.5
    Conserved Domains (1) summary
    pfam02724
    Location:19561
    CDC45; CDC45-like protein

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000022.11 Reference GRCh38.p12 Primary Assembly

    Range
    19479294..19520612
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011530417.3XP_011528719.1  cell division control protein 45 homolog isoform X4

    Conserved Domains (1) summary
    pfam02724
    Location:19516
    CDC45; CDC45-like protein
  2. XM_011530418.3XP_011528720.1  cell division control protein 45 homolog isoform X5

    See identical proteins and their annotated locations for XP_011528720.1

    UniProtKB/Swiss-Prot
    O75419
    Conserved Domains (1) summary
    pfam02724
    Location:19515
    CDC45; CDC45-like protein
  3. XM_011530416.1XP_011528718.1  cell division control protein 45 homolog isoform X2

    Conserved Domains (1) summary
    pfam02724
    Location:7550
    CDC45; CDC45-like protein
  4. XM_024452278.1XP_024308046.1  cell division control protein 45 homolog isoform X3

    Conserved Domains (1) summary
    pfam02724
    Location:7549
    CDC45; CDC45-like protein
  5. XM_024452277.1XP_024308045.1  cell division control protein 45 homolog isoform X1

    Conserved Domains (1) summary
    pfam02724
    Location:19562
    CDC45; CDC45-like protein
  6. XM_017028966.1XP_016884455.1  cell division control protein 45 homolog isoform X6

    Conserved Domains (1) summary
    pfam02724
    Location:4392
    CDC45; CDC45-like protein
  7. XM_017028967.1XP_016884456.1  cell division control protein 45 homolog isoform X7

RNA

  1. XR_002958716.1 RNA Sequence

  2. XR_002958715.1 RNA Sequence

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