PBS3 Auxin-responsive GH3 family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: PBS3
Official Full Name: Auxin-responsive GH3 family protein
Primary source: TAIR:AT5G13320
Locus tag: AT5G13320
See related: Araport:AT5G13320
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AtGH3.12; AVRPPHB SUSCEPTIBLE 3; GDG1; GH3-LIKE DEFENSE GENE 1; GH3.12; GRETCHEN HAGEN 3.12; HOPW1-1-INTERACTING 3; T22N19.5; WIN3
Summary: Encodes an enzyme capable of conjugating amino acids to 4-substituted benzoates. 4-HBA (4-hydroxybenzoic acid) and pABA (4-aminobenzoate) may be targets of the enzyme in Arabidopsis, leading to the production of pABA-Glu, 4HBA-Glu, or other related compounds. This enzyme is involved in disease-resistance signaling. It is required for the accumulation of salicylic acid, activation of defense responses, and resistance to Pseudomonas syringae. Salicylic acid can decrease this enzyme's activity in vitro and may act as a competitive inhibitor. Expression of PBS3/GH3.12 can be detected in cotyledons, true leaves, hypocotyls, and occasionally in some parts of roots from 10-day-old seedlings. No expression has been detected in root, stem, rosette or cauline leaves of mature 4- to 5-week-old plants.
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Genomic context

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See PBS3 in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 6

Sequence:: Chromosome: 5; NC_003076.8 (4267456..4271064)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

GH3 Auxin-Amido Synthetases Alter the Ratio of Indole-3-Acetic Acid and Phenylacetic Acid in Arabidopsis.
Title: GH3 Auxin-Amido Synthetases Alter the Ratio of Indole-3-Acetic Acid and Phenylacetic Acid in Arabidopsis.
PBS3 and EPS1 Complete Salicylic Acid Biosynthesis from Isochorismate in Arabidopsis
Title: PBS3 and EPS1 Complete Salicylic Acid Biosynthesis from Isochorismate in Arabidopsis.
PBS3 Protects EDS1 from Proteasome-Mediated Degradation in Plant Immunity
Title: PBS3 Protects EDS1 from Proteasome-Mediated Degradation in Plant Immunity.
The X-ray crystal structure of GH3.12 is presented in the closed conformation in complex with the nonhydrolysable ATP analogue AMPCPP and the substrate salicylate.
Title: Determination of the GH3.12 protein conformation through HPLC-integrated SAXS measurements combined with X-ray crystallography.
crystal structures of benzoate-specific Arabidopsis thaliana AtGH3.12/PBS3 and jasmonic acid-specific AtGH3.11/JAR1
Title: Structural basis for prereceptor modulation of plant hormones by GH3 proteins.
WIN3 represents a novel node in the salicylic acid signaling networks to regulate plant defense and flowering time. [WIN3]
Title: Multiple roles of WIN3 in regulating disease resistance, cell death, and flowering time in Arabidopsis.
Arabidopsis GH3.12 (PBS3) conjugates amino acids to 4-substituted benzoates and is inhibited by salicylate
Title: Arabidopsis GH3.12 (PBS3) conjugates amino acids to 4-substituted benzoates and is inhibited by salicylate.
WIN3 is partially required for Pseudomonas syringae HopW1-1-induced disease resistance.
Title: Arabidopsis proteins important for modulating defense responses to Pseudomonas syringae that secrete HopW1-1.
WIN3 (At5g13320) is a key element of the RPS2 defense response pathway and a basal and systemic defense component.
Title: A key role for the Arabidopsis WIN3 protein in disease resistance triggered by Pseudomonas syringae that secrete AvrRpt2.
Molecular characterization of the GH3-LIKE DEFENSE GENE 1 (GDG1), a member of the GH3-like gene family, shows that GDG1 is an important component of salicylic acid mediated defense against the bacterial pathogen P. syringae.[GDG1]
Title: Arabidopsis GH3-LIKE DEFENSE GENE 1 is required for accumulation of salicylic acid, activation of defense responses and resistance to Pseudomonas syringae.

Submit: New GeneRIF Correction See all GeneRIFs (11)

General gene information

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NM_001343268.1

Supported by ESTs:

NM_001343269.1

Supported by ESTs:

NM_121335.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables N-(4-aminobenzoyl)-L-glutamate synthetase activity	IDA Inferred from Direct Assay more info	PubMed
enables N-(4-hydroxybenzoyl)-L-glutamate synthetase activity	IDA Inferred from Direct Assay more info	PubMed
enables N-benzoyl-L-glutamate synthetase activity	IDA Inferred from Direct Assay more info	PubMed
enables N-vanillate-L-glutamate synthetase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within benzoate metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to hypoxia	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within defense response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within defense response to bacterium	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within detection of fungus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of plant-type hypersensitive response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of systemic acquired resistance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within salicylic acid mediated signaling pathway	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: Auxin-responsive GH3 family protein

NP_196836.1

AVRPPHB SUSCEPTIBLE 3 (PBS3); CONTAINS InterPro DOMAIN/s: GH3 auxin-responsive promoter (InterPro:IPR004993); BEST Arabidopsis thaliana protein match is: Auxin-responsive GH3 family protein (TAIR:AT1G23160.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

4267456..4271064

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_121335.4 → NP_196836.1 Auxin-responsive GH3 family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_196836.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9LYU4

UniProtKB/TrEMBL

A0A178UEE5

Conserved Domains (1) summary

cl21606
Location:1 → 574

GH3; GH3 auxin-responsive promoter
NM_001343268.1 → NP_001330075.1 Auxin-responsive GH3 family protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A178UEE5, A0A1P8B9P8
NM_001343269.1 → NP_001330076.1 Auxin-responsive GH3 family protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9LYU4

UniProtKB/TrEMBL

A0A178UEE5

Conserved Domains (1) summary

cl21606
Location:1 → 574

GH3; GH3 auxin-responsive promoter