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ACX4 acyl-CoA oxidase 4 [ Arabidopsis thaliana (thale cress) ]

Gene ID: 824347, updated on 4-Feb-2026
Official Symbol
ACX4
Official Full Name
acyl-CoA oxidase 4
Primary source
TAIR:AT3G51840
Locus tag
AT3G51840
See related
Araport:AT3G51840
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
acyl-CoA oxidase 4; ATG6; ATSCX; SHORT-CHAIN ACYL COA OXIDASE
Summary
Encodes a short-chain acyl-CoA oxidase, which catalyzes the first step of peroxisomal fatty acid beta-oxidation during early, post-germinative growth in oilseed species. Null mutants virtually lack short-chain acyl-CoA and are resistant to 2,4-dichlorophenoxybutyric acid, which is converted to the herbicide and auxin analogue 2,4-dichlorophenoxyacetic acid by beta-oxidation. Despite the almost complete loss of short-chain activity, lipid catabolism and seedling growth and establishment was unaltered in the acx4 mutant. However, double mutants in acx3acx4 (acx3 encodes medium chain acyl CoA oxidase) were not viable and arrested during embryogenesis.
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Location:
chromosome: 3
Exon count:
13
Sequence:
Chromosome: 3; NC_003074.8 (19225360..19229341, complement)

Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene UbiA prenyltransferase family protein Neighboring gene SAC domain-containing protein 8 Neighboring gene calcium-dependent protein kinase 13 Neighboring gene cation exchanger 3

Genomic Sequence:
NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Arabidopsis ATG6 is required to limit the pathogen-associated cell death response. Patel S, et al. Autophagy, 2008 Jan. PMID 17932459
  2. Controlling electron transfer in Acyl-CoA oxidases and dehydrogenases: a structural view. Mackenzie J, et al. J Biol Chem, 2006 Oct 13. PMID 16887802
  3. Arabidopsis mutants in short- and medium-chain acyl-CoA oxidase activities accumulate acyl-CoAs and reveal that fatty acid beta-oxidation is essential for embryo development. Rylott EL, et al. J Biol Chem, 2003 Jun 13. PMID 12682048
  4. A novel acyl-CoA oxidase that can oxidize short-chain acyl-CoA in plant peroxisomes. Hayashi H, et al. J Biol Chem, 1999 Apr 30. PMID 10212254
  5. Mutations in Arabidopsis acyl-CoA oxidase genes reveal distinct and overlapping roles in beta-oxidation. Adham AR, et al. Plant J, 2005 Mar. PMID 15743450

See all (13) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that acx3acx4 Columbia (Col) and acx1acx3acx4 (Col) mutants are viable and that enzyme activity in these mutants is significantly reduced on a range of substrates compared to wild type.
    Title: Peroxisomal Acyl-CoA oxidase 4 activity differs between Arabidopsis accessions.
  2. Data suggest that ATG6 is required to limit the pathogen-associated cell death response
    Title: Arabidopsis ATG6 is required to limit the pathogen-associated cell death response.
  3. analysis of electron transfer in Arabidopsis thaliana ACX4
    Title: Controlling electron transfer in Acyl-CoA oxidases and dehydrogenases: a structural view.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description
NM_115043.3

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables acyl-CoA oxidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables acyl-CoA oxidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables salicylic acid binding HDA PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within embryo development ending in seed dormancy IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within fatty acid beta-oxidation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within fatty acid beta-oxidation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within short-chain fatty acid metabolic process TAS
Traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
located_in chloroplast ISM
Inferred from Sequence Model
more info
  
located_in cytosol HDA PubMed 
NOT located_in cytosol RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
located_in peroxisome HDA PubMed 
located_in peroxisome IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
acyl-CoA oxidase 4
NP_190752.1
  • acyl-CoA oxidase 4 (ACX4); FUNCTIONS IN: oxidoreductase activity, acyl-CoA oxidase activity; INVOLVED IN: fatty acid beta-oxidation, short-chain fatty acid metabolic process, embryo development ending in seed dormancy; LOCATED IN: peroxisome; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Acyl-CoA oxidase/dehydrogenase, type1/2, C-terminal (InterPro:IPR013764), Acyl-CoA oxidase/dehydrogenase, type 1 (InterPro:IPR006090), Acyl-CoA dehydrogenase/oxidase (InterPro:IPR009100), Acyl-CoA dehydrogenase/oxidase, N-terminal (InterPro:IPR013786), Acyl-CoA oxidase/dehydrogenase, central domain (InterPro:IPR006091), Acyl-CoA dehydrogenase, conserved site (InterPro:IPR006089), Acyl-CoA dehydrogenase/oxidase C-terminal (InterPro:IPR009075), Acyl-CoA dehydrogenase, N-terminal (InterPro:IPR006092); BEST Arabidopsis thaliana protein match is: isovaleryl-CoA-dehydrogenase (TAIR:AT3G45300.1); Has 36828 Blast hits to 36765 proteins in 1995 species: Archae - 537; Bacteria - 24188; Metazoa - 1639; Fungi - 822; Plants - 341; Viruses - 0; Other Eukaryotes - 9301 (source: NCBI BLink).

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003074.8 Reference assembly

    Range
    19225360..19229341 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_115043.3 → NP_190752.1  acyl-CoA oxidase 4 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_190752.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    Q96329
    UniProtKB/TrEMBL
    A0A5S9XKS0, A0A654FFV7
    Conserved Domains (1) summary
    PLN02526
    Location:27 → 436
    PLN02526; acyl-coenzyme A oxidase
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q96329.1

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