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CANX calnexin [ Homo sapiens (human) ]

Gene ID: 821, updated on 4-Nov-2018

Summary

Official Symbol
CANXprovided by HGNC
Official Full Name
calnexinprovided by HGNC
Primary source
HGNC:HGNC:1473
See related
Ensembl:ENSG00000127022 MIM:114217; Vega:OTTHUMG00000130910
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
CNX; P90; IP90
Summary
This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]
Expression
Ubiquitous expression in thyroid (RPKM 267.4), placenta (RPKM 108.5) and 25 other tissues See more
Orthologs

Genomic context

See CANX in Genome Data Viewer
Location:
5q35.3
Exon count:
18
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 5 NC_000005.10 (179678646..179731641)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 5 NC_000005.9 (179125019..179158642)

Chromosome 5 - NC_000005.10Genomic Context describing neighboring genes Neighboring gene chromosome 5 open reading frame 60 pseudogene Neighboring gene uncharacterized LOC105377763 Neighboring gene chibby family member 3 Neighboring gene high mobility group box 3 pseudogene 22 Neighboring gene mastermind like transcriptional coactivator 1 Neighboring gene NADH:ubiquinone oxidoreductase subunit A12 pseudogene

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Jun 15 11:32:44 2016

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify calnexin (CANX), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
env The signal-sequence-retained form of HIV-1 gp120 is associated with calnexin in the endoplasmic reticulum (ER), while the signal-sequence-cleaved form is disassociated from calnexin and undergoes folding to associate with the CD4 cellular receptor PubMed
Envelope surface glycoprotein gp160, precursor env HIV-1 gp160 interacts with CANX; predicted interaction to be within the endoplasmic reticulum and function as chaperone for endoplasmic reticulum-associated degradation PubMed
env HIV-1 Nef enhances interaction of calnexin with HIV-1 gp160, but inhibits physical interaction between calnexin and ABCA1 PubMed
env HIV-1 gp160 domains V1/V2, C2, and the C-terminus of V3 are required for its binding to calnexin PubMed
env HIV-1 gp160 is identified to have a physical interaction with calnexin (CANX) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
env The molecular chaperone calnexin binds to newly synthesized HIV-1 gp160; nonglycosylated gp160 is not associated with calnexin, suggesting a requirement for N-linked oligosaccharides on the newly synthesized protein for binding to occur PubMed
Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify calnexin (CANX), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Nef nef Knockdown of calnexin by siRNA mimics the effects of HIV-1 Nef on ABCA1 downregulation in cells PubMed
nef HIV-1 Nef physically interacts with calnexin and co-localizes with calnexin to ER membranes in cells PubMed
nef Tandem affinity purification and mass spectrometry analysis identify calnexin (CANX), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Pr55(Gag) gag Tandem affinity purification and mass spectrometry analysis identify calnexin (CANX), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Tat tat Expression of HIV-1 Tat upregulates the abundance of calnexin (CANX) in the nucleoli of Jurkat T-cells PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • Adaptive Immune System, organism-specific biosystem (from REACTOME)
    Adaptive Immune System, organism-specific biosystemAdaptive immunity refers to antigen-specific immune response efficiently involved in clearing the pathogens. The adaptive immune system is comprised of B and T lymphocytes that express receptors with...
  • Antigen Presentation: Folding, assembly and peptide loading of class I MHC, organism-specific biosystem (from REACTOME)
    Antigen Presentation: Folding, assembly and peptide loading of class I MHC, organism-specific biosystemUnlike other glycoproteins, correct folding of MHC class I molecules is not sufficient to trigger their exit from the ER, they exit only after peptide loading. Described here is the process of antige...
  • Antigen processing and presentation, organism-specific biosystem (from KEGG)
    Antigen processing and presentation, organism-specific biosystem
    Antigen processing and presentation
  • Antigen processing and presentation, conserved biosystem (from KEGG)
    Antigen processing and presentation, conserved biosystem
    Antigen processing and presentation
  • Asparagine N-linked glycosylation, organism-specific biosystem (from REACTOME)
    Asparagine N-linked glycosylation, organism-specific biosystemN-linked glycosylation is the most important form of post-translational modification for proteins synthesized and folded in the Endoplasmic Reticulum (Stanley et al. 2009). An early study in 1999 rev...
  • Assembly of Viral Components at the Budding Site, organism-specific biosystem (from REACTOME)
    Assembly of Viral Components at the Budding Site, organism-specific biosystemFollowing synthesis on membrane-bound ribosomes, the three viral integral membrane proteins, HA (hemagglutinin), NA (neuraminidase) and M2 (ion channel) enter the host endoplasmic reticulum (ER) wher...
  • Calnexin/calreticulin cycle, organism-specific biosystem (from REACTOME)
    Calnexin/calreticulin cycle, organism-specific biosystemThe unfolded protein is recognized by a chaperon protein (calnexin or calreticulin) and the folding process starts. The binding of these protein requires a mono-glucosylated glycan (Caramelo JJ and P...
  • Class I MHC mediated antigen processing & presentation, organism-specific biosystem (from REACTOME)
    Class I MHC mediated antigen processing & presentation, organism-specific biosystemMajor histocompatibility complex (MHC) class I molecules play an important role in cell mediated immunity by reporting on intracellular events such as viral infection, the presence of intracellular b...
  • Disease, organism-specific biosystem (from REACTOME)
    Disease, organism-specific biosystemBiological processes are captured in Reactome by identifying the molecules (DNA, RNA, protein, small molecules) involved in them and describing the details of their interactions. From this molecular ...
  • HTLV-I infection, organism-specific biosystem (from KEGG)
    HTLV-I infection, organism-specific biosystemHuman T-lymphotropic virus type 1 (HTLV-1) is a pathogenic retrovirus that is associated with adult T-cell leukemia/lymphoma (ATL). It is also strongly implicated in non-neoplastic chronic inflammato...
  • HTLV-I infection, conserved biosystem (from KEGG)
    HTLV-I infection, conserved biosystemHuman T-lymphotropic virus type 1 (HTLV-1) is a pathogenic retrovirus that is associated with adult T-cell leukemia/lymphoma (ATL). It is also strongly implicated in non-neoplastic chronic inflammato...
  • Immune System, organism-specific biosystem (from REACTOME)
    Immune System, organism-specific biosystemHumans are exposed to millions of potential pathogens daily, through contact, ingestion, and inhalation. Our ability to avoid infection depends on the adaptive immune system and during the first crit...
  • Infectious disease, organism-specific biosystem (from REACTOME)
    Infectious disease, organism-specific biosystem
    Infectious disease
  • Influenza Infection, organism-specific biosystem (from REACTOME)
    Influenza Infection, organism-specific biosystemFor centuries influenza epidemics have plagued man, and influenza was probably the disease described by Hippocrates in 412 BC. Today it remains a major cause of morbidity and mortality worldwide with...
  • Influenza Life Cycle, organism-specific biosystem (from REACTOME)
    Influenza Life Cycle, organism-specific biosystemThe virus particle initially associates with a human host cell by binding to sialic acid-containing receptors on the host cell surface. The bound virus is endocytosed by one of four distinct mechanis...
  • MHC class II antigen presentation, organism-specific biosystem (from REACTOME)
    MHC class II antigen presentation, organism-specific biosystemAntigen presenting cells (APCs) such as B cells, dendritic cells (DCs) and monocytes/macrophages express major histocompatibility complex class II molecules (MHC II) at their surface and present exog...
  • Metabolism of proteins, organism-specific biosystem (from REACTOME)
    Metabolism of proteins, organism-specific biosystemProtein metabolism comprises the pathways of translation, post-translational modification and protein folding.
  • N-glycan trimming in the ER and Calnexin/Calreticulin cycle, organism-specific biosystem (from REACTOME)
    N-glycan trimming in the ER and Calnexin/Calreticulin cycle, organism-specific biosystemAfter being synthesized in the ER membrane the 14-sugars lipid-linked oligosaccharide is co-translationally transferred to an unfolded protein, as described in the previous steps. After this point th...
  • Phagosome, organism-specific biosystem (from KEGG)
    Phagosome, organism-specific biosystemPhagocytosis is the process of taking in relatively large particles by a cell, and is a central mechanism in the tissue remodeling, inflammation, and defense against infectious agents. A phagosome is...
  • Phagosome, conserved biosystem (from KEGG)
    Phagosome, conserved biosystemPhagocytosis is the process of taking in relatively large particles by a cell, and is a central mechanism in the tissue remodeling, inflammation, and defense against infectious agents. A phagosome is...
  • Post-translational protein modification, organism-specific biosystem (from REACTOME)
    Post-translational protein modification, organism-specific biosystemAfter translation, many newly formed proteins undergo further covalent modifications that alter their functional properties and that are essentially irreversible under physiological conditions in the...
  • Protein processing in endoplasmic reticulum, organism-specific biosystem (from KEGG)
    Protein processing in endoplasmic reticulum, organism-specific biosystemThe endoplasmic reticulum (ER) is a subcellular organelle where proteins are folded with the help of lumenal chaperones. Newly synthesized peptides enter the ER via the sec61 pore and are glycosylate...
  • Protein processing in endoplasmic reticulum, conserved biosystem (from KEGG)
    Protein processing in endoplasmic reticulum, conserved biosystemThe endoplasmic reticulum (ER) is a subcellular organelle where proteins are folded with the help of lumenal chaperones. Newly synthesized peptides enter the ER via the sec61 pore and are glycosylate...
  • Thyroid hormone synthesis, organism-specific biosystem (from KEGG)
    Thyroid hormone synthesis, organism-specific biosystemThyroid hormones triiodothyronine (T3) and thyroxine (T4) are essential for normal development, growth and metabolic homeostasis in all vertebrates, and synthesized in the thyroid gland. The function...
  • Thyroid hormone synthesis, conserved biosystem (from KEGG)
    Thyroid hormone synthesis, conserved biosystemThyroid hormones triiodothyronine (T3) and thyroxine (T4) are essential for normal development, growth and metabolic homeostasis in all vertebrates, and synthesized in the thyroid gland. The function...
  • Virus Assembly and Release, organism-specific biosystem (from REACTOME)
    Virus Assembly and Release, organism-specific biosystemInfluenza viruses assemble and bud from the apical plasma membrane of polarized cells e.g. lung epithelial cells of the infected host. This asymmetrical process (i.e. apical [Influenza virus] or baso...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • FLJ26570

Gene Ontology Provided by GOA

Function Evidence Code Pubs
RNA binding HDA PubMed 
apolipoprotein binding IEA
Inferred from Electronic Annotation
more info
 
calcium ion binding IEA
Inferred from Electronic Annotation
more info
 
carbohydrate binding IEA
Inferred from Electronic Annotation
more info
 
ionotropic glutamate receptor binding IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
unfolded protein binding IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
aging IEA
Inferred from Electronic Annotation
more info
 
antigen processing and presentation of exogenous peptide antigen via MHC class II TAS
Traceable Author Statement
more info
 
antigen processing and presentation of peptide antigen via MHC class I TAS
Traceable Author Statement
more info
 
chaperone-mediated protein folding IEA
Inferred from Electronic Annotation
more info
 
clathrin-dependent endocytosis ISS
Inferred from Sequence or Structural Similarity
more info
 
interleukin-27-mediated signaling pathway TAS
Traceable Author Statement
more info
 
interleukin-35-mediated signaling pathway TAS
Traceable Author Statement
more info
 
protein folding TAS
Traceable Author Statement
more info
 
protein folding in endoplasmic reticulum TAS
Traceable Author Statement
more info
PubMed 
protein secretion TAS
Traceable Author Statement
more info
PubMed 
synaptic vesicle endocytosis ISS
Inferred from Sequence or Structural Similarity
more info
 
Component Evidence Code Pubs
Mitochondria-associated ER Membrane IDA
Inferred from Direct Assay
more info
PubMed 
axon IEA
Inferred from Electronic Annotation
more info
 
dendrite cytoplasm IEA
Inferred from Electronic Annotation
more info
 
dendritic spine IEA
Inferred from Electronic Annotation
more info
 
endoplasmic reticulum IDA
Inferred from Direct Assay
more info
PubMed 
endoplasmic reticulum lumen TAS
Traceable Author Statement
more info
 
endoplasmic reticulum membrane IDA
Inferred from Direct Assay
more info
PubMed 
endoplasmic reticulum quality control compartment IEA
Inferred from Electronic Annotation
more info
 
extracellular exosome HDA PubMed 
NOT extracellular exosome IDA
Inferred from Direct Assay
more info
PubMed 
glutamatergic synapse IEA
Inferred from Electronic Annotation
more info
 
integral component of lumenal side of endoplasmic reticulum membrane TAS
Traceable Author Statement
more info
 
integral component of postsynaptic membrane IEA
Inferred from Electronic Annotation
more info
 
integral component of presynaptic active zone membrane IEA
Inferred from Electronic Annotation
more info
 
melanosome IEA
Inferred from Electronic Annotation
more info
 
membrane HDA PubMed 
myelin sheath IEA
Inferred from Electronic Annotation
more info
 
neuronal cell body IEA
Inferred from Electronic Annotation
more info
 
ribosome IEA
Inferred from Electronic Annotation
more info
 
rough endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
 
smooth endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
calnexin
Names
epididymis secretory sperm binding protein
major histocompatibility complex class I antigen-binding protein p88

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001024649.1NP_001019820.1  calnexin isoform d precursor

    See identical proteins and their annotated locations for NP_001019820.1

    Status: REVIEWED

    Source sequence(s)
    AC113426, BC042843
    Consensus CDS
    CCDS4447.1
    UniProtKB/Swiss-Prot
    P27824
    Related
    ENSP00000391646.2, OTTHUMP00000223187, ENST00000452673.6, OTTHUMT00000371747
    Conserved Domains (1) summary
    pfam00262
    Location:71440
    Calreticulin; Calreticulin family
  2. NM_001363993.1NP_001350922.1  calnexin isoform a

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (a).
    Source sequence(s)
    AC113426
    Conserved Domains (1) summary
    pfam00262
    Location:125494
    Calreticulin; Calreticulin family
  3. NM_001363994.1NP_001350923.1  calnexin isoform b

    Status: REVIEWED

    Source sequence(s)
    AC113426, AK294702
    Conserved Domains (1) summary
    pfam00262
    Location:106475
    Calreticulin; Calreticulin family
  4. NM_001363995.1NP_001350924.1  calnexin isoform c precursor

    Status: REVIEWED

    Source sequence(s)
    AC113426
    Conserved Domains (1) summary
    pfam00262
    Location:71474
    Calreticulin; Calreticulin family
  5. NM_001363996.1NP_001350925.1  calnexin isoform d precursor

    Status: REVIEWED

    Source sequence(s)
    AC113426
    Conserved Domains (1) summary
    pfam00262
    Location:71440
    Calreticulin; Calreticulin family
  6. NM_001363997.1NP_001350926.1  calnexin isoform d precursor

    Status: REVIEWED

    Source sequence(s)
    AC113426
    Conserved Domains (1) summary
    pfam00262
    Location:71440
    Calreticulin; Calreticulin family
  7. NM_001363998.1NP_001350927.1  calnexin isoform e precursor

    Status: REVIEWED

    Source sequence(s)
    AC113426
    Conserved Domains (1) summary
    pfam00262
    Location:71440
    Calreticulin; Calreticulin family
  8. NM_001363999.1NP_001350928.1  calnexin isoform f precursor

    Status: REVIEWED

    Source sequence(s)
    AC113426
    Conserved Domains (1) summary
    pfam00262
    Location:71440
    Calreticulin; Calreticulin family
  9. NM_001364000.1NP_001350929.1  calnexin isoform g

    Status: REVIEWED

    Source sequence(s)
    AC113426, AK304420
    Conserved Domains (1) summary
    pfam00262
    Location:6332
    Calreticulin; Calreticulin family
  10. NM_001364001.1NP_001350930.1  calnexin isoform g

    Status: REVIEWED

    Source sequence(s)
    AC113426
    Conserved Domains (1) summary
    pfam00262
    Location:6332
    Calreticulin; Calreticulin family
  11. NM_001746.3NP_001737.1  calnexin isoform d precursor

    See identical proteins and their annotated locations for NP_001737.1

    Status: REVIEWED

    Source sequence(s)
    AC113426, BC003552
    Consensus CDS
    CCDS4447.1
    UniProtKB/Swiss-Prot
    P27824
    Related
    ENSP00000247461.4, OTTHUMP00000161516, ENST00000247461.8, OTTHUMT00000253500
    Conserved Domains (1) summary
    pfam00262
    Location:71440
    Calreticulin; Calreticulin family

RNA

  1. NR_157048.1 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AC113426

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000005.10 Reference GRCh38.p12 Primary Assembly

    Range
    179678646..179731641
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011534665.3XP_011532967.1  calnexin isoform X2

    See identical proteins and their annotated locations for XP_011532967.1

    UniProtKB/Swiss-Prot
    P27824
    Related
    ENSP00000424063.1, OTTHUMP00000223184, ENST00000504734.5
    Conserved Domains (1) summary
    pfam00262
    Location:71440
    Calreticulin; Calreticulin family
  2. XM_024446225.1XP_024301993.1  calnexin isoform X1

  3. XM_011534664.2XP_011532966.1  calnexin isoform X3

    Conserved Domains (1) summary
    pfam00262
    Location:125494
    Calreticulin; Calreticulin family

Reference GRCh38.p12 PATCHES

Genomic

  1. NW_016107298.1 Reference GRCh38.p12 PATCHES

    Range
    464483..497221
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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