dhfr dihydrofolate reductase [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: dhfrprovided by ZNC
Official Full Name: dihydrofolate reductaseprovided by ZNC
Primary source: ZFIN:ZDB-GENE-010406-5
See related: Ensembl:ENSDARG00000004251 AllianceGenome:ZFIN:ZDB-GENE-010406-5
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: cb595; zgc:86637
Summary: Enables dihydrofolate reductase activity. Acts upstream of or within folic acid metabolic process; heart contraction; and heart development. Predicted to be active in mitochondrion. Is expressed in several structures, including anterior neural tube; hematopoietic system; immature eye; nervous system; and neural plate. Human ortholog(s) of this gene implicated in acute lymphoblastic leukemia. Orthologous to several human genes including DHFR (dihydrofolate reductase). [provided by Alliance of Genome Resources, May 2025]
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 5

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2025_04	current	GRCz12tu (GCF_049306965.1)	5	NC_133180.1 (26745864..26755714)
RS_2024_08	previous assembly	GRCz11 (GCF_000002035.6)	5	NC_007116.7 (26138313..26145917)

Chromosome 5 - NC_133180.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

In the current study, we observed the effect of chemical chaperones from four different classes on the stability and functionality of aggregation prone protein zebrafish dihydrofolate reductase
Title: Quantification of differential efficacy of chemical chaperones in ameliorating solubilization and folding of zebrafish dihydrofolate reductase.
The heat induced inactivation of DHFR follows first-order kinetics and Arrhenius law. The variation in the value of inactivation rate constant k with increasing temperatures depicts faster inactivation at elevated temperatures. Attempted to study the chaperoning ability of a shorter variant of GroEL (minichaperone) and compared it with that of conventional GroEL-GroES chaperone system.
Title: Kinetics and thermodynamics of the thermal inactivation and chaperone assisted folding of zebrafish dihydrofolate reductase.
A comparative study of various species of DHFR shows that zDHFR has comparable thermodynamic stability with human counterpart and thus proved to be a good in vitro model system for structure- function relationship studies.
Title: Investigation of folding unfolding process of a new variant of dihydrofolate reductase protein from Zebrafish.
Dihydrofolate reductase is required for the development of heart and outflow tract in zebrafish.
Title: Dihydrofolate reductase is required for the development of heart and outflow tract in zebrafish.

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General gene information

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Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables NADP binding	IBA Inferred from Biological aspect of Ancestor more info
enables NADP binding	IEA Inferred from Electronic Annotation more info
enables dihydrofolate reductase activity	IBA Inferred from Biological aspect of Ancestor more info
enables dihydrofolate reductase activity	IDA Inferred from Direct Assay more info	PubMed
enables dihydrofolate reductase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within bulbus arteriosus development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dihydrofolate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within embryonic morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in folic acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within folic acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heart contraction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heart looping	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heart morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within heart morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within one-carbon metabolic process	IEA Inferred from Electronic Annotation more info
involved_in tetrahydrofolate biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within tetrahydrofolate biosynthetic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: dihydrofolate reductase

NP_571850.1

EC 1.5.1.3

XP_073807243.1

EC 1.5.1.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_131775.1 → NP_571850.1 dihydrofolate reductase

See identical proteins and their annotated locations for NP_571850.1

Status: PROVISIONAL

Source sequence(s)

BC071330

UniProtKB/TrEMBL

Q6IQS4

Related

ENSDARP00000014124.6, ENSDART00000010041.8

Conserved Domains (1) summary

cd00209
Location:5 → 186

DHFR; Dihydrofolate reductase (DHFR). Reduces 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate with NADPH as a cofactor. This is an essential step in the biosynthesis of deoxythymidine phosphate since 5,6,7,8-tetrahydrofolate is required to regenerate 5, ...