Stxbp2 syntaxin binding protein 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Stxbp2provided by RGD
Official Full Name: syntaxin binding protein 2provided by RGD
Primary source: RGD:619967
See related: Ensembl:ENSRNOG00000000994 AllianceGenome:RGD:619967
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Munc18b
Summary: Enables syntaxin-1 binding activity and syntaxin-3 binding activity. Involved in positive regulation of mast cell degranulation and response to acidic pH. Located in apical plasma membrane; lamellipodium; and secretory granule. Part of protein-containing complex. Human ortholog(s) of this gene implicated in familial hemophagocytic lymphohistiocytosis 5. Orthologous to human STXBP2 (syntaxin binding protein 2). [provided by Alliance of Genome Resources, May 2025]
Expression: Biased expression in Kidney (RPKM 123.3), Spleen (RPKM 113.1) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 12p12

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	12	NC_086030.1 (6487265..6498351)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	12	NC_051347.1 (1689364..1701145)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	12	NC_005111.4 (2180101..2191863)

Chromosome 12 - NC_086030.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Munc18-dependent and -independent clustering of syntaxin in the plasma membrane of cultured endocrine cells.
Title: Munc18-dependent and -independent clustering of syntaxin in the plasma membrane of cultured endocrine cells.
SNARE zippering requires activation by SNARE-like peptides in Sec1/Munc18 proteins
Title: SNARE zippering requires activation by SNARE-like peptides in Sec1/Munc18 proteins.
Data show that Munc18b overexpression increased fusion of not only newcomer secretory granule (SG), but also predocked SGs in type-2 diabetes (T2D) human and Goto-Kakizaki Rat Islets.
Title: Munc18b Increases Insulin Granule Fusion, Restoring Deficient Insulin Secretion in Type-2 Diabetes Human and Goto-Kakizaki Rat Islets with Improvement in Glucose Homeostasis.
Secretory vesicles dock by inducing syntaxin1/munc18 clustering in the target membrane.
Title: Contact-induced clustering of syntaxin and munc18 docks secretory granules at the exocytosis site.
Munc18-2 and STX3 perform distinct, but complementary, functions to support, respectively, SG translocation and membrane fusion in mast cells.
Title: Munc18-2 and syntaxin 3 control distinct essential steps in mast cell degranulation.
Munc18b-mediated exocytosis may be deployed to increase secretory efficiency of secretory granules in deeper cytosolic layers of beta-cells as well as additional primary exocytosis
Title: Munc18b is a major mediator of insulin exocytosis in rat pancreatic β-cells.
Double knockdown of Munc18-1 and Munc18-2 in mast cells eliminates both IgE-dependent and ionomycin-induced degranulation and causes a significant reduction in syntaxin-11 without altering expressions of the other syntaxin isoforms examined.
Title: Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation.
The results suggest a mechanism for Cab45b action that involves regulating the dynamic association of Munc18b with SNAREs to impact beta-cell granule exocytosis.
Title: Cab45b, a Munc18b-interacting partner, regulates exocytosis in pancreatic beta-cells.
Results indicate that Munc18-1 and -2 act to support the expression of syntaxins and to deliver syntaxin-1 to the plasma membrane, and that the binding to the closed conformation of syntaxin is essential for Munc18-1 stimulatory action.
Title: Rescue of Munc18-1 and -2 double knockdown reveals the essential functions of interaction between Munc18 and closed syntaxin in PC12 cells.
In a yeast two-hybrid screen the EF-hand Ca(2+)-binding protein Cab45 was identified as an interaction partner of Munc18b.
Title: A cytosolic splice variant of Cab45 interacts with Munc18b and impacts on amylase secretion by pancreatic acini.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables syntaxin binding	IPI Inferred from Physical Interaction more info	PubMed
enables syntaxin-1 binding	IBA Inferred from Biological aspect of Ancestor more info
enables syntaxin-1 binding	IPI Inferred from Physical Interaction more info	PubMed
enables syntaxin-1 binding	ISO Inferred from Sequence Orthology more info
enables syntaxin-3 binding	IBA Inferred from Biological aspect of Ancestor more info
enables syntaxin-3 binding	IEA Inferred from Electronic Annotation more info
enables syntaxin-3 binding	IPI Inferred from Physical Interaction more info	PubMed
enables syntaxin-3 binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cellular response to type II interferon	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within exocytosis	ISO Inferred from Sequence Orthology more info
involved_in intracellular protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in leukocyte mediated cytotoxicity	IEA Inferred from Electronic Annotation more info
involved_in leukocyte mediated cytotoxicity	ISO Inferred from Sequence Orthology more info
involved_in neutrophil degranulation	IEA Inferred from Electronic Annotation more info
involved_in neutrophil degranulation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mast cell degranulation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in presynaptic dense core vesicle exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of mast cell degranulation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to acidic pH	IEP Inferred from Expression Pattern more info	PubMed
involved_in synaptic vesicle exocytosis	TAS Traceable Author Statement more info	PubMed
involved_in vesicle docking involved in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in vesicle-mediated transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in azurophil granule	IEA Inferred from Electronic Annotation more info
located_in azurophil granule	ISO Inferred from Sequence Orthology more info
located_in cytolytic granule	IEA Inferred from Electronic Annotation more info
located_in cytolytic granule	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	IDA Inferred from Direct Assay more info	PubMed
located_in phagocytic vesicle	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in presynapse	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in secretory granule	IBA Inferred from Biological aspect of Ancestor more info
located_in secretory granule	IDA Inferred from Direct Assay more info	PubMed
located_in specific granule	IEA Inferred from Electronic Annotation more info
located_in specific granule	ISO Inferred from Sequence Orthology more info
located_in tertiary granule	IEA Inferred from Electronic Annotation more info
located_in tertiary granule	ISO Inferred from Sequence Orthology more info
is_active_in zymogen granule membrane	ISO Inferred from Sequence Orthology more info
located_in zymogen granule membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: syntaxin-binding protein 2

Names: munc18-2; protein unc-18 homolog 2; protein unc-18 homolog B; syntaxin binding protein Munc18-2; unc-18 homolog 2; unc-18 homolog B; unc-18B; unc18-2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001436421.1 → NP_001423350.1 syntaxin-binding protein 2 isoform 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000012

UniProtKB/Swiss-Prot

Q62753
NM_031126.2 → NP_112388.2 syntaxin-binding protein 2 isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000012

UniProtKB/Swiss-Prot

Q62753

UniProtKB/TrEMBL

A6KQ26

Related

ENSRNOP00000099330.1, ENSRNOT00000155007.1

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086030.1 Reference GRCr8

Range

6487265..6498351

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006248783.5 → XP_006248845.1 syntaxin-binding protein 2 isoform X1

Related

ENSRNOP00000081592.2, ENSRNOT00000115749.2

Conserved Domains (1) summary

pfam00995
Location:29 → 573

Sec1; Sec1 family
XM_063271697.1 → XP_063127767.1 syntaxin-binding protein 2 isoform X2

UniProtKB/Swiss-Prot

Q62753

UniProtKB/TrEMBL

A0A8I5ZT02