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Anpep alanyl aminopeptidase, membrane [ Rattus norvegicus (Norway rat) ]

Gene ID: 81641, updated on 11-Apr-2024

Summary

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Official Symbol
Anpepprovided by RGD
Official Full Name
alanyl aminopeptidase, membraneprovided by RGD
Primary source
RGD:2991
See related
Ensembl:ENSRNOG00000014610 AllianceGenome:RGD:2991
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
Apm; Apn; KZP; AP-M; AP-N; Lap1; rAPN
Summary
Enables aminopeptidase activity and peptide binding activity. Involved in negative regulation of renal sodium excretion; peptide catabolic process; and proteolysis. Located in brush border membrane. Orthologous to human ANPEP (alanyl aminopeptidase, membrane). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Kidney (RPKM 747.7), Lung (RPKM 169.0) and 2 other tissues See more
Orthologs
human mouse all
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Genomic context

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Location:
1q31
Exon count:
23
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 1 NC_086019.1 (143176645..143219447, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 1 NC_051336.1 (133767332..133810137, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 1 NC_005100.4 (141561364..141604249, complement)

Chromosome 1 - NC_086019.1Genomic Context describing neighboring genes Neighboring gene mesoderm posterior bHLH transcription factor 1 Neighboring gene mesoderm posterior bHLH transcription factor 2 Neighboring gene adaptor related protein complex 3 subunit sigma 2 Neighboring gene uncharacterized LOC134483742 Neighboring gene actin-related protein 2/3 complex inhibitor

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. APM/CD13 and FOS in the hypothalamus of monosodium glutamate obese and food deprived rats. Alponti RF, et al. Regul Pept, 2011 Jan 17. PMID 20851150
  2. Intrarenal aminopeptidase N inhibition restores defective angiontesin II type 2-mediated natriuresis in spontaneously hypertensive rats. Padia SH, et al. Hypertension, 2010 Feb. PMID 19996063, Free PMC Article
  3. Functional polymorphism of the Anpep gene increases promoter activity in the Dahl salt-resistant rat. Kotlo K, et al. Hypertension, 2007 Mar. PMID 17242304
  4. First discrete autoradiographic distribution of aminopeptidase N in various structures of rat brain and spinal cord using the selective iodinated inhibitor [125I]RB 129. Noble F, et al. Neuroscience, 2001. PMID 11672613, Free PMC Article
  5. The major APN transcript of the alveolar type II epithelial cell originates from a unique upstream promoter region. Gillis LD, et al. Biochim Biophys Acta, 1998 Aug 20. PMID 9765589

See all (45) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The effect of APN, hs-CRP and APN/hs-CRP in periodontitis with DAA.
    Title: The effect of APN, hs-CRP and APN/hs-CRP in periodontitis with DAA.
  2. In standard light/dark conditions, the results demonstrated an opposite daily rhythm in hypothalamus and pituitary. While the highest levels of AlaAP or enkephalinase activities were measured in hypothalamus during the dark period, they were the highest in the pituitary during the light one.
    Title: Light-dark influence on enkephalinase activity in hypothalamus and pituitary.
  3. Data indicate that fluorogenic substrates can be successfully used to identify aminopeptidase N and to measure their activity in cell lysates.
    Title: Activity profiling of aminopeptidases in cell lysates using a fluorogenic substrate library.
  4. the LAP1 gene (TOR1AIP1) undergoes alternative splicing to originate three LAP1 isoforms (LAP1A, B and C).
    Title: Identification of a novel human LAP1 isoform that is regulated by protein phosphorylation.
  5. the anti-oxidant potential of APN in oxidative stress-associated cardiovascular diseases, such as hypertension-induced HF-preserved EF.
    Title: Adiponectin modulates oxidative stress-induced autophagy in cardiomyocytes.
  6. This study demonistrated that aminopeptidase N were up-regulated ipsilaterally from 6 h to 7 days post ischemia
    Title: Dipeptidyl peptidase IV, aminopeptidase N and DPIV/APN-like proteases in cerebral ischemia.
  7. APN and DPPIV activity levels are related to the development of arthritis, being protective or inducer of the susceptibility
    Title: Neutral aminopeptidase and dipeptidyl peptidase IV in the development of collagen II-induced arthritis.
  8. Results suggest that the upregulation of serotonergic activity may be related to a lowered brain dipeptidyl peptidase III, alanyl- and arginyl aminopeptidase activity which may influence the cleavage of their substrates.
    Title: Central aminopeptidase and serotonin system activities: possible relationship.
  9. hypothalamic CD13 plays a role in the regulation of energy metabolism not by means of puromycin-insensitive membrane-bound neutral aminopeptidase enzyme activity.
    Title: APM/CD13 and FOS in the hypothalamus of monosodium glutamate obese and food deprived rats.
  10. APM and DPPIV-DI are respectively related to the downregulation of somatostatin in food-deprived rats, and to the recovery of energy balance in monosodium glutamate obese rats during food deprivation
    Title: Neutral aminopeptidase and dipeptidyl peptidase IV activities in plasma of monosodium glutamate obese and food-deprived rats.
Submit: New GeneRIF Correction See all GeneRIFs (15)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables aminopeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metalloaminopeptidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables peptide binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables peptide binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in angiogenesis IEA
Inferred from Electronic Annotation
more info
  
involved_in cell differentiation IEA
Inferred from Electronic Annotation
more info
  
involved_in metanephric proximal tubule development IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in peptide catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in peptide catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein processing IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in proteolysis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in brush border membrane IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum-Golgi intermediate compartment IEA
Inferred from Electronic Annotation
more info
  
located_in endoplasmic reticulum-Golgi intermediate compartment ISO
Inferred from Sequence Orthology
more info
  
located_in external side of plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in external side of plasma membrane ISO
Inferred from Sequence Orthology
more info
  
located_in extracellular exosome IEA
Inferred from Electronic Annotation
more info
  
located_in extracellular exosome ISO
Inferred from Sequence Orthology
more info
  
is_active_in extracellular space IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in extracellular space ISO
Inferred from Sequence Orthology
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
  
located_in plasma membrane ISS
Inferred from Sequence or Structural Similarity
more info
  

General protein information

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Preferred Names
aminopeptidase N
Names
alanyl (membrane) aminopeptidase
aminopeptidase M
cluster of differentiation antigen 13 (CD13)
kidney Zn peptidase
kidney aminopeptidase M
leucine arylaminopeptidase 1
membrane alanine aminopeptidase
microsomal aminopeptidase
NP_112274.1
XP_006229469.1
XP_006229470.1
XP_006229471.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_031012.1 → NP_112274.1  aminopeptidase N precursor

    See identical proteins and their annotated locations for NP_112274.1

    Status: PROVISIONAL

    Source sequence(s)
    M25073
    UniProtKB/Swiss-Prot
    P15684, Q9JHP4
    UniProtKB/TrEMBL
    A0A0G2JVE6
    Related
    ENSRNOP00000020002.5, ENSRNOT00000020002.6
    Conserved Domains (3) summary
    cd09601
    Location:84 → 550
    M1_APN_2; Peptidase M1 Aminopeptidase N family incudes tricorn interacting factor F3, Endoplasmic reticulum aminopeptidase 1 (ERAP1), Aminopeptidase Q (APQ)
    pfam01433
    Location:75 → 479
    Peptidase_M1; Peptidase family M1
    pfam11838
    Location:619 → 942
    ERAP1_C; ERAP1-like C-terminal domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086019.1 Reference GRCr8

    Range
    143176645..143219447 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006229409.3 → XP_006229471.1  aminopeptidase N isoform X1

    See identical proteins and their annotated locations for XP_006229471.1

    UniProtKB/TrEMBL
    A0A0G2JVE6, A6JC72, G3V7W7
    Conserved Domains (3) summary
    cd09601
    Location:84 → 550
    M1_APN_2; Peptidase M1 Aminopeptidase N family incudes tricorn interacting factor F3, Endoplasmic reticulum aminopeptidase 1 (ERAP1), Aminopeptidase Q (APQ)
    pfam01433
    Location:75 → 479
    Peptidase_M1; Peptidase family M1
    pfam11838
    Location:619 → 942
    ERAP1_C; ERAP1-like C-terminal domain

  2. XM_006229407.5 → XP_006229469.1  aminopeptidase N isoform X1

    See identical proteins and their annotated locations for XP_006229469.1

    UniProtKB/TrEMBL
    A0A0G2JVE6, A6JC72, G3V7W7
    Conserved Domains (3) summary
    cd09601
    Location:84 → 550
    M1_APN_2; Peptidase M1 Aminopeptidase N family incudes tricorn interacting factor F3, Endoplasmic reticulum aminopeptidase 1 (ERAP1), Aminopeptidase Q (APQ)
    pfam01433
    Location:75 → 479
    Peptidase_M1; Peptidase family M1
    pfam11838
    Location:619 → 942
    ERAP1_C; ERAP1-like C-terminal domain

  3. XM_006229408.5 → XP_006229470.1  aminopeptidase N isoform X1

    See identical proteins and their annotated locations for XP_006229470.1

    UniProtKB/TrEMBL
    A0A0G2JVE6, A6JC72, G3V7W7
    Conserved Domains (3) summary
    cd09601
    Location:84 → 550
    M1_APN_2; Peptidase M1 Aminopeptidase N family incudes tricorn interacting factor F3, Endoplasmic reticulum aminopeptidase 1 (ERAP1), Aminopeptidase Q (APQ)
    pfam01433
    Location:75 → 479
    Peptidase_M1; Peptidase family M1
    pfam11838
    Location:619 → 942
    ERAP1_C; ERAP1-like C-terminal domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P15684.2 GenPept UniProtKB/Swiss-Prot:P15684

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