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TSSK3 testis specific serine kinase 3 [ Homo sapiens (human) ]

Gene ID: 81629, updated on 25-Nov-2025
Official Symbol
TSSK3provided by HGNC
Official Full Name
testis specific serine kinase 3provided by HGNC
Primary source
HGNC:HGNC:15473
See related
Ensembl:ENSG00000162526 MIM:607660; AllianceGenome:HGNC:15473
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
TSK3; SPOGA3; STK22C; STK22D
Summary
This gene encodes a kinase expressed exclusively in the testis that is thought to play a role in either germ cell differentiation or mature sperm function. [provided by RefSeq, Jul 2008]
Expression
Broad expression in testis (RPKM 12.3), bone marrow (RPKM 7.8) and 23 other tissues See more
Orthologs
mouse all
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See TSSK3 in Genome Data Viewer
Location:
1p35.1
Exon count:
2
Annotation release Status Assembly Chr Location
RS_2025_08 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (32362539..32364312)
RS_2025_08 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (32220454..32222227)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (32828140..32829913)

Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC124903949 Neighboring gene uncharacterized LOC105378629 Neighboring gene Sharpr-MPRA regulatory region 14076 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 596 Neighboring gene uncharacterized LOC124903933 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:32818736-32819250 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 597 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 598 Neighboring gene family with sequence similarity 229 member A Neighboring gene BSD domain containing 1 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:32859083-32859780 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:32859781-32860476 Neighboring gene H3K27ac hESC enhancer GRCh37_chr1:32861175-32861870 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 689 Neighboring gene glyceraldehyde 3 phosphate dehydrogenase pseudogene 20

Go to nucleotide: Graphics FASTA GenBank

  • Project title: Tissue-specific circular RNA induction during human fetal development
  • Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
  • BioProject: PRJNA270632
  • Publication: PMID 26076956
  • Analysis date: Mon Apr 2 22:54:59 2018

Related articles in PubMed

  1. TSSK3, a novel target for male contraception, is required for spermiogenesis. Nayyab S, et al. Mol Reprod Dev, 2021 Nov. PMID 34623009, Free PMC Article
  2. Cloning and chromosomal localization of a gene encoding a novel serine/threonine kinase belonging to the subfamily of testis-specific kinases. Visconti PE, et al. Genomics, 2001 Oct. PMID 11597141
  3. Characterization of testis-specific serine-threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling. Bucko-Justyna M, et al. FEBS J, 2005 Dec. PMID 16336268
  4. Protein microarrays for the identification of praja1 e3 ubiquitin ligase substrates. Loch CM, et al. Cell Biochem Biophys, 2011 Jun. PMID 21461837
  5. Quantitative analysis of HSP90-client interactions reveals principles of substrate recognition. Taipale M, et al. Cell, 2012 Aug 31. PMID 22939624, Free PMC Article

See all (16) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. TSSK3, a novel target for male contraception, is required for spermiogenesis.
    Title: TSSK3, a novel target for male contraception, is required for spermiogenesis.
  2. PDK1-induced phosphorylation increased in vitro TSSK3 kinase activity, suggesting that TSSK3 can be regulated in the same way as AGC kinase family members. The peptide sequence RRSSSY containing Ser5 is identified as a target for TSSK3 phosphorylation
    Title: Characterization of testis-specific serine-threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling.
  3. human homolog of mouse stk22d; It was cloned and found it to be expressed exclusively in the testis.
    Title: Cloning and chromosomal localization of a gene encoding a novel serine/threonine kinase belonging to the subfamily of testis-specific kinases.
Submit: New GeneRIF Correction

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP binding ISS
Inferred from Sequence or Structural Similarity
more info
  
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding ISS
Inferred from Sequence or Structural Similarity
more info
  
enables manganese ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein serine/threonine kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein serine/threonine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine/threonine kinase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in cell differentiation IEA
Inferred from Electronic Annotation
more info
  
involved_in protein phosphorylation ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in spermatid development IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in spermatid development IEA
Inferred from Electronic Annotation
more info
  
involved_in spermatid development ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in spermatogenesis IEA
Inferred from Electronic Annotation
more info
  
involved_in spermatogenesis ISS
Inferred from Sequence or Structural Similarity
more info
  
Component Evidence Code Pubs
located_in motile cilium IEA
Inferred from Electronic Annotation
more info
  
is_active_in sperm flagellum IEA
Inferred from Electronic Annotation
more info
  
is_active_in sperm flagellum ISS
Inferred from Sequence or Structural Similarity
more info
  
Preferred Names
testis-specific serine/threonine-protein kinase 3
Names
TSK-3
TSSK-3
serine/threonine-protein kinase 22C
spermiogenesis associated 3
testis-specific kinase 3
testis-specific serine/threonine kinase 22C
NP_443073.1

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_052841.4 → NP_443073.1  testis-specific serine/threonine-protein kinase 3

    See identical proteins and their annotated locations for NP_443073.1

    Status: REVIEWED

    Source sequence(s)
    BC035354
    Consensus CDS
    CCDS362.1
    UniProtKB/Swiss-Prot
    Q5TEE5, Q96PN8
    Related
    ENSP00000362634.3, ENST00000373534.4
    Conserved Domains (2) summary
    smart00220
    Location:10 → 265
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd14163
    Location:9 → 265
    STKc_TSSK3-like; Catalytic domain of testis-specific serine/threonine kinase 3 and similar proteins

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2025_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

    Range
    32362539..32364312
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060925.1 Alternate T2T-CHM13v2.0

    Range
    32220454..32222227
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q96PN8.1 GenPept UniProtKB/Swiss-Prot:Q96PN8

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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