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WEE1 WEE1 G2 checkpoint kinase [ Homo sapiens (human) ]

Gene ID: 7465, updated on 7-Jul-2019

Summary

Official Symbol
WEE1provided by HGNC
Official Full Name
WEE1 G2 checkpoint kinaseprovided by HGNC
Primary source
HGNC:HGNC:12761
See related
Ensembl:ENSG00000166483 MIM:193525
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
WEE1A; WEE1hu
Summary
This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in gall bladder (RPKM 23.7), esophagus (RPKM 19.4) and 22 other tissues See more
Orthologs

Genomic context

See WEE1 in Genome Data Viewer
Location:
11p15.4
Exon count:
12
Annotation release Status Assembly Chr Location
109.20190607 current GRCh38.p13 (GCF_000001405.39) 11 NC_000011.10 (9573670..9589985)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 11 NC_000011.9 (9595197..9615016)

Chromosome 11 - NC_000011.10Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC644656 Neighboring gene zinc finger protein 143 Neighboring gene ribosomal protein L23a pseudogene 65 Neighboring gene ribosomal protein L21 pseudogene 97

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Vpr vpr Prolonged cell cycle arrest in the G2 phase induced by HIV-1 Vpr results in the depletion of Wee1 kinase PubMed
vpr HIV-1 Vpr binds to Wee1. Amino acids 291-369 in Wee1 are required for the binding between Vpr and Wee1. Vpr stimulates Wee1 kinase activity PubMed
vpr HIV-1 Vpr-induced cell cycle arrest results in increased levels of Wee-1 kinase in synchronized HeLa cells; G2 arrest by Vpr is correlated with delayed degradation of Wee-1, and Cdc2 activity is reduced at G2/M in the presence of Vpr PubMed
vpr HIV-1 Vpr induced cell cycle arrest in the G2 phase is mediated predominately through the Wee1 kinase in fission yeast PubMed
vpr Overexpression of Wee-1 kinase suppresses HIV-1 Vpr-mediated apoptosis PubMed
vpr Rupture of HIV-1 Vpr-induced nuclear envelope herniations produces transient loss of the subcellular compartmentalization of Wee1, Cdc25C, cyclin B1, and presumably other soluble cellular components, resulting in their release into the cytoplasm PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • Cell Cycle, organism-specific biosystem (from WikiPathways)
    Cell Cycle, organism-specific biosystemThe cell cycle is the series of events that takes place in a cell leading to its division and duplication (replication). Regulation of the cell cycle involves processes crucial to the survival of a c...
  • Cell Cycle, organism-specific biosystem (from REACTOME)
    Cell Cycle, organism-specific biosystem
    Cell Cycle
  • Cell Cycle Checkpoints, organism-specific biosystem (from REACTOME)
    Cell Cycle Checkpoints, organism-specific biosystemA hallmark of the human cell cycle in normal somatic cells is its precision. This remarkable fidelity is achieved by a number of signal transduction pathways, known as checkpoints, which monitor cell...
  • Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
    Cell Cycle, Mitotic, organism-specific biosystemThe replication of the genome and the subsequent segregation of chromosomes into daughter cells are controlled by a series of events collectively known as the cell cycle. DNA replication is carried o...
  • Cell cycle, organism-specific biosystem (from KEGG)
    Cell cycle, organism-specific biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cycli...
  • Cell cycle, conserved biosystem (from KEGG)
    Cell cycle, conserved biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cycli...
  • Cell cycle - G2/M transition, organism-specific biosystem (from KEGG)
    Cell cycle - G2/M transition, organism-specific biosystemFunctional set; Cellular processes; Cell signaling
  • Cell cycle - G2/M transition, conserved biosystem (from KEGG)
    Cell cycle - G2/M transition, conserved biosystemFunctional set; Cellular processes; Cell signaling
  • Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex, organism-specific biosystem (from REACTOME)
    Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex, organism-specific biosystemDNA damage induced activation of the checkpoint kinases Chk1/Chk2(Cds1) results in the conversion and/or maintenance of CyclinB:Cdc2 complex in its Tyrosine 15 phosphorylated (inactive) state. Cdc2...
  • Cyclin A/B1 associated events during G2/M transition, organism-specific biosystem (from REACTOME)
    Cyclin A/B1 associated events during G2/M transition, organism-specific biosystemCell cycle progression is regulated by cyclin-dependent protein kinases at both the G1/S and the G2/M transitions. The G2/M transition is regulated through the phosphorylation of nuclear lamins and h...
  • Cyclin A:Cdk2-associated events at S phase entry, organism-specific biosystem (from REACTOME)
    Cyclin A:Cdk2-associated events at S phase entry, organism-specific biosystemCyclin A:Cdk2 plays a key role in S phase entry by phosphorylation of proteins including Cdh1, Rb, p21 and p27. During G1 phase of the cell cycle, cyclin A is synthesized and associates with Cdk2. Af...
  • Cyclin E associated events during G1/S transition, organism-specific biosystem (from REACTOME)
    Cyclin E associated events during G1/S transition, organism-specific biosystemThe transition from the G1 to S phase is controlled by the Cyclin E:Cdk2 complexes. As the Cyclin E:Cdk2 complexes are formed, the Cdk2 is phosphorylated by the Wee1 and Myt1 kinases. This phosphoryl...
  • Factors involved in megakaryocyte development and platelet production, organism-specific biosystem (from REACTOME)
    Factors involved in megakaryocyte development and platelet production, organism-specific biosystemMegakaryocytes (MKs) give rise to circulating platelets (thrombocytes) through terminal differentiation of MKs which release cytoplasmic fragments as circulating platelets. As MKs mature they underg...
  • G1 to S cell cycle control, organism-specific biosystem (from WikiPathways)
    G1 to S cell cycle control, organism-specific biosystemIn the G1 phase there are two types of DNA damage responses, the p53-dependent and the p53-independent pathways. The p53-dependent responses inhibit CDKs through the up-regulation of genes encoding C...
  • G1/S Transition, organism-specific biosystem (from REACTOME)
    G1/S Transition, organism-specific biosystemCyclin E - Cdk2 complexes control the transition from G1 into S-phase. In this case, the binding of p21Cip1/Waf1 or p27kip1 is inhibitory. Important substrates for Cyclin E - Cdk2 complexes include p...
  • G2/M Checkpoints, organism-specific biosystem (from REACTOME)
    G2/M Checkpoints, organism-specific biosystemG2/M checkpoints include the checks for damaged DNA, unreplicated DNA, and checks that ensure that the genome is replicated once and only once per cell cycle. If cells pass these checkpoints, they f...
  • G2/M DNA damage checkpoint, organism-specific biosystem (from REACTOME)
    G2/M DNA damage checkpoint, organism-specific biosystemThroughout the cell cycle, the genome is constantly monitored for damage, resulting either from errors of replication, by-products of metabolism or through extrinsic sources such as ultra-violet or i...
  • G2/M DNA replication checkpoint, organism-specific biosystem (from REACTOME)
    G2/M DNA replication checkpoint, organism-specific biosystemThe G2/M DNA replication checkpoint ensures that mitosis is not initiated until DNA replication is complete. If replication is blocked, the DNA replication checkpoint signals to maintain Cyclin B - C...
  • G2/M Transition, organism-specific biosystem (from REACTOME)
    G2/M Transition, organism-specific biosystemCyclin A can also form complexes with Cdc2 (Cdk1). Together with three B-type cyclins, Cdc2 (Cdk1) regulates the transition from G2 into mitosis. These complexes are activated by dephosphorylation of...
  • Hemostasis, organism-specific biosystem (from REACTOME)
    Hemostasis, organism-specific biosystemHemostasis is a physiological response that culminates in the arrest of bleeding from an injured vessel. Under normal conditions the vascular endothelium supports vasodilation, inhibits platelet adhe...
  • Integrated Breast Cancer Pathway, organism-specific biosystem (from WikiPathways)
    Integrated Breast Cancer Pathway, organism-specific biosystemThis pathway incorporates the most important proteins for Breast Cancer. The Rp score from the Connectivity-Maps (C-Maps) webserver was used to determine the rank of the most important proteins in Br...
  • Mitotic G1-G1/S phases, organism-specific biosystem (from REACTOME)
    Mitotic G1-G1/S phases, organism-specific biosystem
    Mitotic G1-G1/S phases
  • Mitotic G2-G2/M phases, organism-specific biosystem (from REACTOME)
    Mitotic G2-G2/M phases, organism-specific biosystem
    Mitotic G2-G2/M phases
  • PLK1 signaling events, organism-specific biosystem (from Pathway Interaction Database)
    PLK1 signaling events, organism-specific biosystem
    PLK1 signaling events
  • Polo-like kinase mediated events, organism-specific biosystem (from REACTOME)
    Polo-like kinase mediated events, organism-specific biosystemAt mitotic entry, Plk1 phosphorylates and activates Cdc25C phosphatase, whereas it phosphorylates and down-regulates Wee1A (Watanabe et al. 2004). Plk1 also phosphorylates and inhibits Myt1 activit...
  • Retinoblastoma (RB) in Cancer, organism-specific biosystem (from WikiPathways)
    Retinoblastoma (RB) in Cancer, organism-specific biosystemDescribes the role of retinoblastoma (RB) gene in cancer.
  • S Phase, organism-specific biosystem (from REACTOME)
    S Phase, organism-specific biosystemDNA synthesis occurs in the S phase, or the synthesis phase, of the cell cycle. The cell duplicates its hereditary material, and two copies of the chromosome are formed. As DNA replication continues,...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • FLJ16446, DKFZp686I18166

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
kinase activity TAS
Traceable Author Statement
more info
 
magnesium ion binding IEA
Inferred from Electronic Annotation
more info
 
non-membrane spanning protein tyrosine kinase activity IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
protein tyrosine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Process Evidence Code Pubs
G2/M transition of mitotic cell cycle TAS
Traceable Author Statement
more info
 
cell division IEA
Inferred from Electronic Annotation
more info
 
establishment of cell polarity IEA
Inferred from Electronic Annotation
more info
 
microtubule cytoskeleton organization IEA
Inferred from Electronic Annotation
more info
 
mitotic cell cycle checkpoint IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
negative regulation of G1/S transition of mitotic cell cycle TAS
Traceable Author Statement
more info
 
neuron projection morphogenesis IEA
Inferred from Electronic Annotation
more info
 
peptidyl-tyrosine phosphorylation IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
cytoplasm IEA
Inferred from Electronic Annotation
more info
 
nucleolus IDA
Inferred from Direct Assay
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 

General protein information

Preferred Names
wee1-like protein kinase
Names
WEE1 homolog
WEE1+ homolog
wee1A kinase
NP_001137448.1
NP_003381.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001143976.1NP_001137448.1  wee1-like protein kinase isoform 2

    See identical proteins and their annotated locations for NP_001137448.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) has a different first exon and 5' UTR, compared to variant 1. This difference causes translation initiation from an in-frame downstream AUG and an isoform (2) with a shorter N-terminus, compared to isoform 1.
    Source sequence(s)
    AK122837, BQ024511
    Consensus CDS
    CCDS44536.1
    UniProtKB/Swiss-Prot
    P30291
    Related
    ENSP00000299613.5, ENST00000299613.10
    Conserved Domains (2) summary
    cd14138
    Location:79354
    PTKc_Wee1a; Catalytic domain of the Protein Tyrosine Kinase, Wee1a
    pfam00069
    Location:85355
    Pkinase; Protein kinase domain
  2. NM_003390.4NP_003381.1  wee1-like protein kinase isoform 1

    See identical proteins and their annotated locations for NP_003381.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AC011979, AC122179, BC051831, BQ024511, U10564
    Consensus CDS
    CCDS7800.1
    UniProtKB/Swiss-Prot
    P30291
    UniProtKB/TrEMBL
    Q86V29
    Related
    ENSP00000402084.2, ENST00000450114.7
    Conserved Domains (2) summary
    cd14138
    Location:293568
    PTKc_Wee1a; Catalytic domain of the Protein Tyrosine Kinase, Wee1a
    pfam00069
    Location:299569
    Pkinase; Protein kinase domain

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000011.10 Reference GRCh38.p13 Primary Assembly

    Range
    9573670..9589985
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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