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Robo4 roundabout guidance receptor 4 [ Mus musculus (house mouse) ]

Gene ID: 74144, updated on 12-Feb-2023

Summary

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Official Symbol
Robo4provided by MGI
Official Full Name
roundabout guidance receptor 4provided by MGI
Primary source
MGI:MGI:1921394
See related
Ensembl:ENSMUSG00000032125 AllianceGenome:MGI:1921394
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
1200012D01Rik
Summary
Enables signaling receptor activity. Acts upstream of or within negative regulation of cell migration. Located in external side of plasma membrane. Is expressed in several structures, including forebrain; genitourinary system; neural retina; submandibular gland; and vascular system. Human ortholog(s) of this gene implicated in aortic valve disease 3. Orthologous to human ROBO4 (roundabout guidance receptor 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in lung adult (RPKM 31.0), subcutaneous fat pad adult (RPKM 26.6) and 19 other tissues See more
Orthologs
human all
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Genomic context

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See Robo4 in Genome Data Viewer
Location:
9; 9 A4
Exon count:
17
Annotation release Status Assembly Chr Location
109 current GRCm39 (GCF_000001635.27) 9 NC_000075.7 (37313198..37325319)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 9 NC_000075.6 (37401902..37414023)

Chromosome 9 - NC_000075.7Genomic Context describing neighboring genes Neighboring gene coiled-coil domain containing 15 Neighboring gene predicted gene, 34885 Neighboring gene hepatocyte cell adhesion molecule Neighboring gene roundabout guidance receptor 3 Neighboring gene Myb/SANT-like DNA-binding domain containing 2

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions. Adzraku SY, et al. Cell Mol Biol Lett, 2023 Jan 16. PMID 36647012, Free PMC Article
  2. Upregulation of Robo4 expression by SMAD signaling suppresses vascular permeability and mortality in endotoxemia and COVID-19 models. Morita M, et al. Proc Natl Acad Sci U S A, 2023 Jan 17. PMID 36634143, Free PMC Article
  3. [The destructive role of soluble Robo4 secreted by the M1-polarized-microglia during cerebral ischemia-reperfusion in blood-brain barrier integrity]. Huang JL, et al. Sheng Li Xue Bao, 2022 Aug 25. PMID 35993202
  4. Robo4-mediated pancreatic endothelial integrity decreases inflammation and islet destruction in autoimmune diabetes. Troullinaki M, et al. FASEB J, 2020 Feb. PMID 31916652
  5. Deletion of Robo4 prevents high-fat diet-induced adipose artery and systemic metabolic dysfunction. Phuong TTT, et al. Microcirculation, 2019 Jul. PMID 30825241, Free PMC Article

See all (62) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Upregulation of Robo4 expression by SMAD signaling suppresses vascular permeability and mortality in endotoxemia and COVID-19 models.
    Title: Upregulation of Robo4 expression by SMAD signaling suppresses vascular permeability and mortality in endotoxemia and COVID-19 models.
  2. Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions.
    Title: Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions.
  3. [The destructive role of soluble Robo4 secreted by the M1-polarized-microglia during cerebral ischemia-reperfusion in blood-brain barrier integrity].
    Title: [The destructive role of soluble Robo4 secreted by the M1-polarized-microglia during cerebral ischemia-reperfusion in blood-brain barrier integrity].
  4. ROBO4 deletion ameliorates PAF-mediated skin inflammation via regulating the mRNA translation efficiency of LPCAT1/LPCAT2 and the expression of PAF receptor.
    Title: ROBO4 deletion ameliorates PAF-mediated skin inflammation via regulating the mRNA translation efficiency of LPCAT1/LPCAT2 and the expression of PAF receptor.
  5. Robo4-mediated pancreatic endothelial integrity decreases inflammation and islet destruction in autoimmune diabetes.
    Title: Robo4-mediated pancreatic endothelial integrity decreases inflammation and islet destruction in autoimmune diabetes.
  6. a novel functional role for Robo4 in endothelial cell function and metabolic homeostasis in white adipose tissue, with Robo4 deletion protecting against endothelial and metabolic dysfunction associated with a HFD.
    Title: Deletion of Robo4 prevents high-fat diet-induced adipose artery and systemic metabolic dysfunction.
  7. Robo4 inhibits angiogenesis and vessel permeability independently of its cytoplasmic domain. Robo4 deletion enhances permeability and revascularization in oxygen-induced retinopathy (OIR) and accelerates cutaneous wound healing.
    Title: The Robo4 cytoplasmic domain is dispensable for vascular permeability and neovascularization.
  8. endothelial Robo4 signaling is important for suppressing breast cancer growth and metastasis, and it can be targeted (enhanced) by administrating a small molecular drug.
    Title: Endothelial Robo4 suppresses breast cancer growth and metastasis through regulation of tumor angiogenesis.
  9. Robo4 vaccines induce antibodies that retard tumor growth.
    Title: Robo4 vaccines induce antibodies that retard tumor growth.
  10. Robo4 and Src family kinases are involved in the mechanisms of embryonic vascular defects caused by the Hrt/Hey deficiency.
    Title: An important role of endothelial hairy-related transcription factors in mouse vascular development.
Submit: New GeneRIF Correction See all GeneRIFs (20)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (8)  1 citation
  • Endonuclease-mediated (2) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables signaling receptor activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in angiogenesis ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of blood vessel endothelial cell migration IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within cell differentiation IEA
Inferred from Electronic Annotation
more info
  
involved_in cell-cell adhesion IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
involved_in establishment of endothelial barrier ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within multicellular organism development IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within negative regulation of blood vessel endothelial cell migration IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in external side of plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane TAS
Traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
roundabout homolog 4
Names
Magic roundabout

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001309390.2 → NP_001296319.2  roundabout homolog 4 isoform 1 precursor

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AC138284
    Consensus CDS
    CCDS80976.1
    UniProtKB/Swiss-Prot
    Q9DBW1
    Related
    ENSMUSP00000150722.3, ENSMUST00000214185.3
    Conserved Domains (2) summary
    cd00063
    Location:351 → 436
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cl11960
    Location:142 → 226
    Ig; Immunoglobulin domain

  2. NM_028783.4 → NP_083059.3  roundabout homolog 4 isoform 2 precursor

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
    Source sequence(s)
    AC138284
    Consensus CDS
    CCDS22977.1
    Related
    ENSMUSP00000159318.2, ENSMUST00000239486.2
    Conserved Domains (2) summary
    cd00063
    Location:351 → 436
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cl11960
    Location:142 → 226
    Ig; Immunoglobulin domain

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000075.7 Reference GRCm39 C57BL/6J

    Range
    37313198..37325319
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006510644.1 → XP_006510707.1  roundabout homolog 4 isoform X1

    UniProtKB/TrEMBL
    D3Z4M4
    Related
    ENSMUSP00000110698.3, ENSMUST00000115046.9
    Conserved Domains (2) summary
    cd00063
    Location:361 → 446
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cl11960
    Location:152 → 236
    Ig; Immunoglobulin domain

  2. XM_006510645.2 → XP_006510708.1  roundabout homolog 4 isoform X2

    Conserved Domains (2) summary
    cd00063
    Location:361 → 446
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cl11960
    Location:152 → 236
    Ig; Immunoglobulin domain

  3. XM_036155356.1 → XP_036011249.1  roundabout homolog 4 isoform X3

    Conserved Domains (3) summary
    pfam00041
    Location:348 → 430
    fn3; Fibronectin type III domain
    cd00096
    Location:138 → 141
    Ig; Ig strand A [structural motif]
    cl11960
    Location:138 → 223
    Ig; Immunoglobulin domain

  4. XM_036155358.1 → XP_036011251.1  roundabout homolog 4 isoform X5

    Conserved Domains (3) summary
    pfam00041
    Location:158 → 240
    fn3; Fibronectin type III domain
    cd00096
    Location:11 → 19
    Ig; Ig strand F [structural motif]
    cl11960
    Location:1 → 33
    Ig; Immunoglobulin domain

  5. XM_011242615.2 → XP_011240917.1  roundabout homolog 4 isoform X4

    Conserved Domains (3) summary
    pfam00041
    Location:158 → 240
    fn3; Fibronectin type III domain
    cd00096
    Location:11 → 19
    Ig; Ig strand F [structural motif]
    cl11960
    Location:1 → 33
    Ig; Immunoglobulin domain

  6. XM_036155357.1 → XP_036011250.1  roundabout homolog 4 isoform X4

    Conserved Domains (3) summary
    pfam00041
    Location:158 → 240
    fn3; Fibronectin type III domain
    cd00096
    Location:11 → 19
    Ig; Ig strand F [structural motif]
    cl11960
    Location:1 → 33
    Ig; Immunoglobulin domain

  7. XM_006510647.3 → XP_006510710.1  roundabout homolog 4 isoform X6

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8C310.2 GenPept UniProtKB/Swiss-Prot:Q8C310

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