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TOP2B DNA topoisomerase II beta [ Homo sapiens (human) ]

Gene ID: 7155, updated on 7-Jul-2019

Summary

Official Symbol
TOP2Bprovided by HGNC
Official Full Name
DNA topoisomerase II betaprovided by HGNC
Primary source
HGNC:HGNC:11990
See related
Ensembl:ENSG00000077097 MIM:126431
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
TOPIIB; top2beta
Summary
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Expression
Ubiquitous expression in urinary bladder (RPKM 32.3), brain (RPKM 31.7) and 25 other tissues See more
Orthologs

Genomic context

See TOP2B in Genome Data Viewer
Location:
3p24.2
Exon count:
36
Annotation release Status Assembly Chr Location
109.20190607 current GRCh38.p13 (GCF_000001405.39) 3 NC_000003.12 (25597905..25664891, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 3 NC_000003.11 (25639396..25706396, complement)

Chromosome 3 - NC_000003.12Genomic Context describing neighboring genes Neighboring gene retinoic acid receptor beta Neighboring gene RNA, 5S ribosomal pseudogene 126 Neighboring gene RARB antisense RNA 1 Neighboring gene NUP98-TOP2B recombination region Neighboring gene microRNA 4442 Neighboring gene cysteine rich protein 1 pseudogene 2 Neighboring gene ribosomal protein L32 pseudogene 11 Neighboring gene N-glycanase 1

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
HIV-1 replication is enhanced by over-expression of TOP2B (TopoisomeraseIIb) PubMed
HIV-1 replication requires TOP2B (TopoisomeraseIIb) as shown through inhibition by ICRF-193 or merbarone treatment or siRNA knockdown PubMed
Knockdown of TOP2A, TOP2B, or TOP2A/2B by siRNA significantly decreases HIV-1 replication in Sup T1 cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Tat tat HIV-1 Tat binds TOP2B (topoisomeraseIIb), XRCC6 (Ku70), and PARP1 during transactivation and viral replication PubMed
capsid gag Knockdown of TOP2A, TOP2B, or TOP2A/2B by siRNA significantly decreases p24 levels in HIV-1 infected cell culture supernatants when compared to control PubMed
reverse transcriptase gag-pol HIV-1 RT co-localizes with TOP2A and TOP2B in cytosol of HIV-1 infected Sup T1 and PBMC cells PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • Metabolism of proteins, organism-specific biosystem (from REACTOME)
    Metabolism of proteins, organism-specific biosystemProtein metabolism comprises the pathways of translation, post-translational modification and protein folding.
  • Platinum drug resistance, organism-specific biosystem (from KEGG)
    Platinum drug resistance, organism-specific biosystemPlatinum-based drugs cisplatin, carboplatin and oxaliplatin are widely used in the therapy of solid malignancies, including testicular, ovarian, head and neck, colorectal, bladder and lung cancers. T...
  • Platinum drug resistance, conserved biosystem (from KEGG)
    Platinum drug resistance, conserved biosystemPlatinum-based drugs cisplatin, carboplatin and oxaliplatin are widely used in the therapy of solid malignancies, including testicular, ovarian, head and neck, colorectal, bladder and lung cancers. T...
  • Post-translational protein modification, organism-specific biosystem (from REACTOME)
    Post-translational protein modification, organism-specific biosystemAfter translation, many newly formed proteins undergo further covalent modifications that alter their functional properties and that are essentially irreversible under physiological conditions in the...
  • SUMO E3 ligases SUMOylate target proteins, organism-specific biosystem (from REACTOME)
    SUMO E3 ligases SUMOylate target proteins, organism-specific biosystemSUMO proteins are conjugated to lysine residues of target proteins via an isopeptide bond with the C-terminal glycine of SUMO (reviewed in Zhao 2007, Gareau and Lima 2010, Hannoun et al. 2010, Citro ...
  • SUMOylation, organism-specific biosystem (from REACTOME)
    SUMOylation, organism-specific biosystemSmall Ubiquitin-like MOdifiers (SUMOs) are a family of 3 proteins (SUMO1,2,3) that are reversibly conjugated to lysine residues of target proteins via a glycine-lysine isopeptide bond (reviewed in Ha...
  • SUMOylation of DNA replication proteins, organism-specific biosystem (from REACTOME)
    SUMOylation of DNA replication proteins, organism-specific biosystemThe sliding clamp protein PCNA, Aurora-A, Aurora-B, Borealin, and various topoisomerases can be SUMOylated (reviewed in Wan et al. 2012). SUMOylation of PCNA appears to reduce formation of double-str...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
DNA binding IEA
Inferred from Electronic Annotation
more info
 
DNA topoisomerase type II (ATP-hydrolyzing) activity IDA
Inferred from Direct Assay
more info
PubMed 
chromatin binding IDA
Inferred from Direct Assay
more info
PubMed 
enzyme binding IPI
Inferred from Physical Interaction
more info
PubMed 
histone deacetylase binding IPI
Inferred from Physical Interaction
more info
PubMed 
metal ion binding IEA
Inferred from Electronic Annotation
more info
 
protein C-terminus binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein heterodimerization activity IPI
Inferred from Physical Interaction
more info
PubMed 
protein kinase C binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
DNA topological change IDA
Inferred from Direct Assay
more info
PubMed 
axonogenesis IEA
Inferred from Electronic Annotation
more info
 
forebrain development IEA
Inferred from Electronic Annotation
more info
 
mitotic DNA integrity checkpoint IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
neuron migration IEA
Inferred from Electronic Annotation
more info
 
positive regulation of single stranded viral RNA replication via double stranded DNA intermediate IMP
Inferred from Mutant Phenotype
more info
PubMed 
resolution of meiotic recombination intermediates IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
sister chromatid segregation IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Component Evidence Code Pubs
cytosol IDA
Inferred from Direct Assay
more info
PubMed 
colocalizes_with heterochromatin IDA
Inferred from Direct Assay
more info
PubMed 
NOT nucleolus IDA
Inferred from Direct Assay
more info
PubMed 
nucleoplasm IDA
Inferred from Direct Assay
more info
PubMed 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
ribonucleoprotein complex ISS
Inferred from Sequence or Structural Similarity
more info
 
viral integration complex IMP
Inferred from Mutant Phenotype
more info
PubMed 

General protein information

Preferred Names
DNA topoisomerase 2-beta
Names
DNA topoisomerase II, 180 kD
DNA topoisomerase II, beta isozyme
U937 associated antigen
antigen MLAA-44
topo II beta
topoisomerase (DNA) II beta 180kDa
topoisomerase II beta
topoisomerase IIb
NP_001059.2
NP_001317629.1
XP_011532359.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_052961.1 RefSeqGene

    Range
    4874..71468
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001068.3NP_001059.2  DNA topoisomerase 2-beta isoform 2

    See identical proteins and their annotated locations for NP_001059.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) contains an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.
    Source sequence(s)
    AB208879, BF514624, BM150930, X68060
    Consensus CDS
    CCDS46776.1
    UniProtKB/Swiss-Prot
    Q02880
    UniProtKB/TrEMBL
    Q59H80
    Related
    ENSP00000396704.2, ENST00000435706.6
    Conserved Domains (7) summary
    cd03365
    Location:471591
    TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...
    cd00187
    Location:7271187
    TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes in DNA ...
    cd03481
    Location:281433
    TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...
    PTZ00108
    Location:411466
    PTZ00108; DNA topoisomerase 2-like protein; Provisional
    pfam02518
    Location:96193
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
    pfam08070
    Location:15031604
    DTHCT; DTHCT (NUC029) region
    pfam16898
    Location:589727
    TOPRIM_C; C-terminal associated domain of TOPRIM
  2. NM_001330700.1NP_001317629.1  DNA topoisomerase 2-beta isoform 1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AC093416
    Consensus CDS
    CCDS82746.1
    UniProtKB/Swiss-Prot
    Q02880
    Related
    ENSP00000264331.4, ENST00000264331.8

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000003.12 Reference GRCh38.p13 Primary Assembly

    Range
    25597905..25664891 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011534057.3XP_011532359.1  DNA topoisomerase 2-beta isoform X1

    Conserved Domains (7) summary
    cd03365
    Location:476596
    TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...
    cd00187
    Location:7321192
    TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes in DNA ...
    cd03481
    Location:286438
    TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...
    PTZ00108
    Location:461466
    PTZ00108; DNA topoisomerase 2-like protein; Provisional
    pfam02518
    Location:101198
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
    pfam08070
    Location:14711572
    DTHCT; DTHCT (NUC029) region
    pfam16898
    Location:594732
    TOPRIM_C; C-terminal associated domain of TOPRIM

RNA

  1. XR_001740241.2 RNA Sequence

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