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TOP2A DNA topoisomerase II alpha [ Homo sapiens (human) ]

Gene ID: 7153, updated on 8-Jul-2018
Official Symbol
TOP2Aprovided by HGNC
Official Full Name
DNA topoisomerase II alphaprovided by HGNC
Primary source
HGNC:HGNC:11989
See related
Ensembl:ENSG00000131747 MIM:126430; Vega:OTTHUMG00000155008
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
TOP2; TP2A
Summary
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]
Expression
Biased expression in testis (RPKM 34.8), lymph node (RPKM 21.9) and 13 other tissues See more
Orthologs
See TOP2A in Genome Data Viewer
Location:
17q21.2
Exon count:
36
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 17 NC_000017.11 (40388521..40417950, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (38544773..38574202, complement)

Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene RNA, 5S ribosomal pseudogene 441 Neighboring gene kelch repeat and BTB domain containing 2 pseudogene Neighboring gene ribosomal protein L23a pseudogene 75 Neighboring gene insulin like growth factor binding protein 4

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Jun 15 11:32:44 2016

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Replication interactions

Interaction Pubs
Knockdown of TOP2A, TOP2B, or TOP2A/2B by siRNA significantly decreases HIV-1 replication in Sup T1 cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120 is identified to have a physical interaction with topoisomerase (DNA) II alpha 170kDa (TOP2A) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
Tat tat Interaction of HIV-1 Tat with TOP2A in T-cells is identified by a proteomic strategy based on affinity chromatography coupled with mass spectrometry PubMed
capsid gag Knockdown of TOP2A, TOP2B, or TOP2A/2B by siRNA significantly decreases p24 levels in HIV-1 infected cell culture supernatants when compared to control PubMed
reverse transcriptase gag-pol HIV-1 RT co-localizes with TOP2A and TOP2B in cytosol of HIV-1 infected Sup T1 and PBMC cells PubMed

Go to the HIV-1, Human Interaction Database

  • Cell Cycle, organism-specific biosystem (from REACTOME)
    Cell Cycle, organism-specific biosystem
    Cell Cycle
  • Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
    Cell Cycle, Mitotic, organism-specific biosystemThe replication of the genome and the subsequent segregation of chromosomes into daughter cells are controlled by a series of events collectively known as the cell cycle. DNA replication is carried o...
  • Circadian rythm related genes, organism-specific biosystem (from WikiPathways)
    Circadian rythm related genes, organism-specific biosystemThis is currently not a pathway but a list of circadian rhythm related genes and proteins. The source for this information is the gene ontology. The genes and proteins were filtered for "circadian rh...
  • G0 and Early G1, organism-specific biosystem (from REACTOME)
    G0 and Early G1, organism-specific biosystemIn G0 and early G1 in quiescent cells, p130 (RBL2) bound to E2F4 or E2F5 and either DP1 or DP2, associates with the MuvB complex, forming an evolutionarily conserved DREAM complex, that represses tra...
  • Gastric Cancer Network 1, organism-specific biosystem (from WikiPathways)
    Gastric Cancer Network 1, organism-specific biosystemNetwork generated by mapping candidate oncogenes and tumor suppressor genes identified by integrated analysis of expression array and aCGH data. Networks generated by Ingenuity Pathway Analysis.
  • Gastric Cancer Network 2, organism-specific biosystem (from WikiPathways)
    Gastric Cancer Network 2, organism-specific biosystemNetwork generated by mapping candidate oncogenes and tumor suppressor genes identified by integrated analysis of expression array and aCGH data. Network generated by Ingenuity Pathway Analysis.
  • Integrated Pancreatic Cancer Pathway, organism-specific biosystem (from WikiPathways)
    Integrated Pancreatic Cancer Pathway, organism-specific biosystemAn integrated pathway model which displays the protein-protein interactions (PPIs) among the relevant proteins for pancreatic cancer. This pathway is a collection of different mechanistic protein pat...
  • Metabolism of proteins, organism-specific biosystem (from REACTOME)
    Metabolism of proteins, organism-specific biosystemProtein metabolism comprises the pathways of translation, post-translational modification and protein folding.
  • Mitotic G1-G1/S phases, organism-specific biosystem (from REACTOME)
    Mitotic G1-G1/S phases, organism-specific biosystem
    Mitotic G1-G1/S phases
  • Platinum drug resistance, organism-specific biosystem (from KEGG)
    Platinum drug resistance, organism-specific biosystemPlatinum-based drugs cisplatin, carboplatin and oxaliplatin are widely used in the therapy of solid malignancies, including testicular, ovarian, head and neck, colorectal, bladder and lung cancers. T...
  • Platinum drug resistance, conserved biosystem (from KEGG)
    Platinum drug resistance, conserved biosystemPlatinum-based drugs cisplatin, carboplatin and oxaliplatin are widely used in the therapy of solid malignancies, including testicular, ovarian, head and neck, colorectal, bladder and lung cancers. T...
  • Post-translational protein modification, organism-specific biosystem (from REACTOME)
    Post-translational protein modification, organism-specific biosystemAfter translation, many newly formed proteins undergo further covalent modifications that alter their functional properties and that are essentially irreversible under physiological conditions in the...
  • Retinoblastoma (RB) in Cancer, organism-specific biosystem (from WikiPathways)
    Retinoblastoma (RB) in Cancer, organism-specific biosystemDescribes the role of retinoblastoma (RB) gene in cancer.
  • SUMO E3 ligases SUMOylate target proteins, organism-specific biosystem (from REACTOME)
    SUMO E3 ligases SUMOylate target proteins, organism-specific biosystemSUMO proteins are conjugated to lysine residues of target proteins via an isopeptide bond with the C-terminal glycine of SUMO (reviewed in Zhao 2007, Gareau and Lima 2010, Hannoun et al. 2010, Citro ...
  • SUMOylation, organism-specific biosystem (from REACTOME)
    SUMOylation, organism-specific biosystemSmall Ubiquitin-like MOdifiers (SUMOs) are a family of 3 proteins (SUMO1,2,3) that are reversibly conjugated to lysine residues of target proteins via a glycine-lysine isopeptide bond (reviewed in Ha...
  • SUMOylation of DNA replication proteins, organism-specific biosystem (from REACTOME)
    SUMOylation of DNA replication proteins, organism-specific biosystemThe sliding clamp protein PCNA, Aurora-A, Aurora-B, Borealin, and various topoisomerases can be SUMOylated (reviewed in Wan et al. 2012). SUMOylation of PCNA appears to reduce formation of double-str...
Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
DNA binding, bending IDA
Inferred from Direct Assay
more info
PubMed 
DNA topoisomerase type II (ATP-hydrolyzing) activity IDA
Inferred from Direct Assay
more info
PubMed 
DNA topoisomerase type II (ATP-hydrolyzing) activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
DNA-dependent ATPase activity IDA
Inferred from Direct Assay
more info
PubMed 
RNA binding HDA PubMed 
chromatin binding IDA
Inferred from Direct Assay
more info
PubMed 
drug binding IDA
Inferred from Direct Assay
more info
PubMed 
enzyme binding IPI
Inferred from Physical Interaction
more info
PubMed 
histone deacetylase binding IPI
Inferred from Physical Interaction
more info
PubMed 
magnesium ion binding IDA
Inferred from Direct Assay
more info
PubMed 
protein C-terminus binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein heterodimerization activity IPI
Inferred from Physical Interaction
more info
PubMed 
protein homodimerization activity IPI
Inferred from Physical Interaction
more info
PubMed 
protein kinase C binding IPI
Inferred from Physical Interaction
more info
PubMed 
ubiquitin binding IMP
Inferred from Mutant Phenotype
more info
PubMed 
Process Evidence Code Pubs
DNA ligation IDA
Inferred from Direct Assay
more info
PubMed 
DNA topological change IDA
Inferred from Direct Assay
more info
PubMed 
DNA topological change IMP
Inferred from Mutant Phenotype
more info
PubMed 
apoptotic chromosome condensation IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
apoptotic chromosome condensation IDA
Inferred from Direct Assay
more info
PubMed 
cellular response to DNA damage stimulus IDA
Inferred from Direct Assay
more info
PubMed 
chromosome segregation IMP
Inferred from Mutant Phenotype
more info
PubMed 
embryonic cleavage IEA
Inferred from Electronic Annotation
more info
 
hematopoietic progenitor cell differentiation IEA
Inferred from Electronic Annotation
more info
 
mitotic DNA integrity checkpoint IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
negative regulation of DNA duplex unwinding IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of apoptotic process IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of single stranded viral RNA replication via double stranded DNA intermediate IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of transcription by RNA polymerase II IEA
Inferred from Electronic Annotation
more info
 
positive regulation of viral genome replication IMP
Inferred from Mutant Phenotype
more info
PubMed 
regulation of circadian rhythm ISS
Inferred from Sequence or Structural Similarity
more info
 
resolution of meiotic recombination intermediates IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
rhythmic process IEA
Inferred from Electronic Annotation
more info
 
sister chromatid segregation IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Component Evidence Code Pubs
DNA topoisomerase complex (ATP-hydrolyzing) IDA
Inferred from Direct Assay
more info
PubMed 
colocalizes_with centriole IDA
Inferred from Direct Assay
more info
PubMed 
condensed chromosome IEA
Inferred from Electronic Annotation
more info
 
colocalizes_with nuclear chromosome IDA
Inferred from Direct Assay
more info
PubMed 
nucleolus IDA
Inferred from Direct Assay
more info
PubMed 
NOT nucleolus IDA
Inferred from Direct Assay
more info
PubMed 
nucleoplasm IDA
Inferred from Direct Assay
more info
PubMed 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
protein-containing complex IDA
Inferred from Direct Assay
more info
PubMed 
ribonucleoprotein complex IDA
Inferred from Direct Assay
more info
PubMed 
colocalizes_with viral integration complex NAS
Non-traceable Author Statement
more info
PubMed 
Preferred Names
DNA topoisomerase 2-alpha
Names
DNA gyrase
DNA topoisomerase (ATP-hydrolyzing)
DNA topoisomerase II, 170 kD
DNA topoisomerase II, alpha isozyme
topoisomerase (DNA) II alpha 170kDa
NP_001058.2
XP_005257689.1
XP_011523467.1

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_027678.1 RefSeqGene

    Range
    5001..34430
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001067.3NP_001058.2  DNA topoisomerase 2-alpha

    See identical proteins and their annotated locations for NP_001058.2

    Status: REVIEWED

    Source sequence(s)
    AC080112, BC140791, DB069825
    Consensus CDS
    CCDS45672.1
    UniProtKB/Swiss-Prot
    P11388
    Related
    ENSP00000411532.1, OTTHUMP00000207108, ENST00000423485.5, OTTHUMT00000338035
    Conserved Domains (8) summary
    cd03365
    Location:455575
    TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...
    cd00187
    Location:7111174
    TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes in DNA ...
    cd03481
    Location:265417
    TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...
    PTZ00108
    Location:251497
    PTZ00108; DNA topoisomerase 2-like protein; Provisional
    pfam02518
    Location:80185
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
    pfam08070
    Location:14351521
    DTHCT; DTHCT (NUC029) region
    pfam15323
    Location:13571461
    Ashwin; Developmental protein
    pfam16898
    Location:573711
    TOPRIM_C; C-terminal associated domain of TOPRIM

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000017.11 Reference GRCh38.p12 Primary Assembly

    Range
    40388521..40417950 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_005257632.1XP_005257689.1  DNA topoisomerase 2-alpha isoform X1

    Conserved Domains (8) summary
    cd03365
    Location:443563
    TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...
    cd00187
    Location:6991162
    TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes in DNA ...
    cd03481
    Location:253405
    TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...
    PTZ00108
    Location:131485
    PTZ00108; DNA topoisomerase 2-like protein; Provisional
    pfam02518
    Location:68173
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
    pfam08070
    Location:14231509
    DTHCT; DTHCT (NUC029) region
    pfam15323
    Location:13451449
    Ashwin; Developmental protein
    pfam16898
    Location:561699
    TOPRIM_C; C-terminal associated domain of TOPRIM
  2. XM_011525165.2XP_011523467.1  DNA topoisomerase 2-alpha isoform X2

    Conserved Domains (6) summary
    cd03365
    Location:455575
    TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...
    cd00187
    Location:7111174
    TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes in DNA ...
    cd03481
    Location:265417
    TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...
    PTZ00108
    Location:251364
    PTZ00108; DNA topoisomerase 2-like protein; Provisional
    pfam02518
    Location:80185
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
    pfam16898
    Location:573711
    TOPRIM_C; C-terminal associated domain of TOPRIM
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