Trim32 tripartite motif-containing 32 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Trim32provided by MGI
Official Full Name: tripartite motif-containing 32provided by MGI
Primary source: MGI:MGI:1917057
See related: Ensembl:ENSMUSG00000051675 AllianceGenome:MGI:1917057
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 3f3; BBS11; Zfp117; 1810045E12Rik
Summary: Enables several functions, including identical protein binding activity; translation initiation factor binding activity; and ubiquitin protein ligase activity. Involved in several processes, including positive regulation of NF-kappaB transcription factor activity; positive regulation of cell differentiation; and response to tumor necrosis factor. Acts upstream of or within several processes, including positive regulation of cytokine-mediated signaling pathway; positive regulation of macromolecule metabolic process; and protein ubiquitination. Located in nucleus and striated muscle myosin thick filament. Is expressed in genitourinary system. Used to study autosomal recessive limb-girdle muscular dystrophy type 2H. Human ortholog(s) of this gene implicated in Bardet-Biedl syndrome; Bardet-Biedl syndrome 11; autosomal recessive limb-girdle muscular dystrophy type 2H; and muscular dystrophy. Orthologous to human TRIM32 (tripartite motif containing 32). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in CNS E18 (RPKM 39.0), whole brain E14.5 (RPKM 30.7) and 23 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 C1; 4 34.43 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (65523223..65534477)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (65604986..65616240)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Tripartite motif-containing protein 32 regulates Ca[2+] movement in skeletal muscle.
Title: Tripartite motif-containing protein 32 regulates Ca(2+) movement in skeletal muscle.
TRIM32 Inhibition Attenuates Apoptosis, Oxidative Stress, and Inflammatory Injury in Podocytes Induced by High Glucose by Modulating the Akt/GSK-3beta/Nrf2 Pathway.
Title: TRIM32 Inhibition Attenuates Apoptosis, Oxidative Stress, and Inflammatory Injury in Podocytes Induced by High Glucose by Modulating the Akt/GSK-3β/Nrf2 Pathway.
TRIM32 Deficiency Impairs the Generation of Pyramidal Neurons in Developing Cerebral Cortex.
Title: TRIM32 Deficiency Impairs the Generation of Pyramidal Neurons in Developing Cerebral Cortex.
TRIM32 Deficiency Impairs Synaptic Plasticity by Excitatory-Inhibitory Imbalance via Notch Pathway.
Title: TRIM32 Deficiency Impairs Synaptic Plasticity by Excitatory-Inhibitory Imbalance via Notch Pathway.
Inverse Correlation of TRIM32 and Protein Kinase C zeta in T Helper Type 2-Biased Inflammation.
Title: Inverse Correlation of TRIM32 and Protein Kinase C ζ in T Helper Type 2-Biased Inflammation.
Trim32 suppresses cerebellar development and tumorigenesis by degrading Gli1/sonic hedgehog signaling.
Title: Trim32 suppresses cerebellar development and tumorigenesis by degrading Gli1/sonic hedgehog signaling.
These results suggest that TRIM32 sensitizes Streptococcus suis-induced infection via innate immune response regulation.
Title: TRIM32 Drives Pathogenesis in Streptococcal Toxic Shock-Like Syndrome and Streptococcus suis Meningitis by Regulating Innate Immune Responses.
TRIM32 associates with the deubiquitination enzyme USP7, which previously has been implicated in neural stem cell maintenance. USP7 and TRIM32 were found to exhibit a dynamic and partially overlapping expression pattern during neuronal differentiation both in vitro and in vivo.
Title: A complex of the ubiquitin ligase TRIM32 and the deubiquitinase USP7 balances the level of c-Myc ubiquitination and thereby determines neural stem cell fate specification.
The proautophagic function of TRIM32 relies on its ability to bind the autophagy proteins AMBRA1 and ULK1 and stimulate ULK1 activity via unanchored K63-linked polyubiquitin.
Title: Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains.
the miRNA activity inhibition function of pathogenic LRRK2 is directly antagonized by the neuronal cell fate determinant TRIM32.
Title: Parkinson's Disease-Associated Mutant LRRK2-Mediated Inhibition of miRNA Activity is Antagonized by TRIM32.

Submit: New GeneRIF Correction See all GeneRIFs (34)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1) 1 citation
Targeted (2) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Homologs of the Trim32 gene: The Trim32 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, zebrafish, and frog.
Orthologs from Annotation Pipeline: 488 organisms have orthologs with human gene Trim32
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables myosin binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein self-association	ISO Inferred from Sequence Orthology more info
enables transcription coactivator activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables translation initiation factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within actin ubiquitination	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within axon development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fat cell differentiation	IEP Inferred from Expression Pattern more info	PubMed
involved_in free ubiquitin chain polymerization	ISO Inferred from Sequence Orthology more info
involved_in innate immune response	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within muscle cell cellular homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of fibroblast proliferation	IEP Inferred from Expression Pattern more info	PubMed
involved_in negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of keratinocyte apoptotic process	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of viral transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of I-kappaB kinase/NF-kappaB signaling	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NF-kappaB transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of autophagy	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell cycle	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell growth	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell motility	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of chemokine (C-C motif) ligand 20 production	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of interleukin-17-mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of neurogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of neuron differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of proteolysis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of striated muscle cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of tumor necrosis factor-mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein K48-linked ubiquitination	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of_or_within protein polyubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein polyubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in response to UV	IDA Inferred from Direct Assay more info	PubMed
involved_in response to tumor necrosis factor	IDA Inferred from Direct Assay more info	PubMed
involved_in suppression of viral release by host	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within tissue homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in striated muscle myosin thick filament	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: E3 ubiquitin-protein ligase TRIM32

Names: RING-type E3 ubiquitin transferase TRIM32; bM468K2.2 (tripartite motif protein 32); tripartite motif protein 32; tripartite motif-containing protein 32; zinc finger protein 117

NP_001155254.1

EC 2.3.2.27

NP_444314.2

EC 2.3.2.27

XP_006538298.1

EC 2.3.2.27

XP_006538299.1

EC 2.3.2.27

XP_006538300.1

EC 2.3.2.27

XP_006538301.1

EC 2.3.2.27

XP_030109625.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001161782.1 → NP_001155254.1 E3 ubiquitin-protein ligase TRIM32

See identical proteins and their annotated locations for NP_001155254.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.

Source sequence(s)

BC034104, BE855341, BG073447, BY280460

Consensus CDS

CCDS18270.1

UniProtKB/Swiss-Prot

Q8CH72, Q8K055

UniProtKB/TrEMBL

A0A8C6H804, Q3TLR3

Related

ENSMUSP00000102989.2, ENSMUST00000107366.2

Conserved Domains (3) summary

cd14961
Location:364 → 646

NHL_TRIM32_like; NHL repeat domain of the tripartite motif-containing protein 32 (TRIM32) and related proteins

cd16587
Location:20 → 66

RING-HC_TRIM32_C-VII; RING finger, HC subclass, found in tripartite motif-containing protein 32 (TRIM32) and similar proteins

cd19806
Location:99 → 139

Bbox1_TRIM32_C-VII; B-box-type 1 zinc finger found in tripartite motif-containing protein 32 (TRIM32) and similar proteins
NM_053084.2 → NP_444314.2 E3 ubiquitin-protein ligase TRIM32

See identical proteins and their annotated locations for NP_444314.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.

Source sequence(s)

BC034104, BE855341, BG073447, BY280460

Consensus CDS

CCDS18270.1

UniProtKB/Swiss-Prot

Q8CH72, Q8K055

UniProtKB/TrEMBL

A0A8C6H804, Q3TLR3

Related

ENSMUSP00000062277.8, ENSMUST00000050850.14

Conserved Domains (3) summary

cd14961
Location:364 → 646

NHL_TRIM32_like; NHL repeat domain of the tripartite motif-containing protein 32 (TRIM32) and related proteins

cd16587
Location:20 → 66

RING-HC_TRIM32_C-VII; RING finger, HC subclass, found in tripartite motif-containing protein 32 (TRIM32) and similar proteins

cd19806
Location:99 → 139

Bbox1_TRIM32_C-VII; B-box-type 1 zinc finger found in tripartite motif-containing protein 32 (TRIM32) and similar proteins

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000070.7 Reference GRCm39 C57BL/6J

Range

65523223..65534477

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006538235.2 → XP_006538298.1 E3 ubiquitin-protein ligase TRIM32 isoform X1

See identical proteins and their annotated locations for XP_006538298.1

UniProtKB/Swiss-Prot

Q8CH72, Q8K055

UniProtKB/TrEMBL

A0A8C6H804, Q3TLR3

Conserved Domains (3) summary

cd14961
Location:364 → 646

NHL_TRIM32_like; NHL repeat domain of the tripartite motif-containing protein 32 (TRIM32) and related proteins

cd16587
Location:20 → 66

RING-HC_TRIM32_C-VII; RING finger, HC subclass, found in tripartite motif-containing protein 32 (TRIM32) and similar proteins

cd19806
Location:99 → 139

Bbox1_TRIM32_C-VII; B-box-type 1 zinc finger found in tripartite motif-containing protein 32 (TRIM32) and similar proteins
XM_006538238.3 → XP_006538301.1 E3 ubiquitin-protein ligase TRIM32 isoform X1

See identical proteins and their annotated locations for XP_006538301.1

UniProtKB/Swiss-Prot

Q8CH72, Q8K055

UniProtKB/TrEMBL

A0A8C6H804, Q3TLR3

Conserved Domains (3) summary

cd14961
Location:364 → 646

NHL_TRIM32_like; NHL repeat domain of the tripartite motif-containing protein 32 (TRIM32) and related proteins

cd16587
Location:20 → 66

RING-HC_TRIM32_C-VII; RING finger, HC subclass, found in tripartite motif-containing protein 32 (TRIM32) and similar proteins

cd19806
Location:99 → 139

Bbox1_TRIM32_C-VII; B-box-type 1 zinc finger found in tripartite motif-containing protein 32 (TRIM32) and similar proteins
XM_006538236.5 → XP_006538299.1 E3 ubiquitin-protein ligase TRIM32 isoform X1

See identical proteins and their annotated locations for XP_006538299.1

UniProtKB/Swiss-Prot

Q8CH72, Q8K055

UniProtKB/TrEMBL

A0A8C6H804, Q3TLR3

Conserved Domains (3) summary

cd14961
Location:364 → 646

NHL_TRIM32_like; NHL repeat domain of the tripartite motif-containing protein 32 (TRIM32) and related proteins

cd16587
Location:20 → 66

RING-HC_TRIM32_C-VII; RING finger, HC subclass, found in tripartite motif-containing protein 32 (TRIM32) and similar proteins

cd19806
Location:99 → 139

Bbox1_TRIM32_C-VII; B-box-type 1 zinc finger found in tripartite motif-containing protein 32 (TRIM32) and similar proteins
XM_006538237.2 → XP_006538300.1 E3 ubiquitin-protein ligase TRIM32 isoform X1

See identical proteins and their annotated locations for XP_006538300.1

UniProtKB/Swiss-Prot

Q8CH72, Q8K055

UniProtKB/TrEMBL

A0A8C6H804, Q3TLR3

Conserved Domains (3) summary

cd14961
Location:364 → 646

NHL_TRIM32_like; NHL repeat domain of the tripartite motif-containing protein 32 (TRIM32) and related proteins

cd16587
Location:20 → 66

RING-HC_TRIM32_C-VII; RING finger, HC subclass, found in tripartite motif-containing protein 32 (TRIM32) and similar proteins

cd19806
Location:99 → 139

Bbox1_TRIM32_C-VII; B-box-type 1 zinc finger found in tripartite motif-containing protein 32 (TRIM32) and similar proteins
XM_030253765.2 → XP_030109625.1 E3 ubiquitin-protein ligase TRIM32 isoform X1

UniProtKB/Swiss-Prot

Q8K055

UniProtKB/TrEMBL

A0A8C6H804, Q3TLR3

Conserved Domains (3) summary

cd14961
Location:364 → 646

NHL_TRIM32_like; NHL repeat domain of the tripartite motif-containing protein 32 (TRIM32) and related proteins

cd16587
Location:20 → 66

RING-HC_TRIM32_C-VII; RING finger, HC subclass, found in tripartite motif-containing protein 32 (TRIM32) and similar proteins

cd19806
Location:99 → 139

Bbox1_TRIM32_C-VII; B-box-type 1 zinc finger found in tripartite motif-containing protein 32 (TRIM32) and similar proteins