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Alkbh8 alkB homolog 8, tRNA methyltransferase [ Mus musculus (house mouse) ]

Gene ID: 67667, updated on 7-Apr-2025

Summary

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Official Symbol
Alkbh8provided by MGI
Official Full Name
alkB homolog 8, tRNA methyltransferaseprovided by MGI
Primary source
MGI:MGI:1914917
See related
Ensembl:ENSMUSG00000025899 AllianceGenome:MGI:1914917
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Abh8; 4930562C03Rik; 8030431D03Rik; 9430088N01Rik
Summary
Enables iron ion binding activity and tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity. Involved in tRNA methylation and tRNA wobble uridine modification. Predicted to be located in cytosol and nucleoplasm. Predicted to be active in cytoplasm and nucleus. Is expressed in several structures, including blastocyst; early embryo; nervous system; secondary oocyte; and sensory organ. Human ortholog(s) of this gene implicated in autosomal recessive intellectual developmental disorder 71. Orthologous to human ALKBH8 (alkB homolog 8, tRNA methyltransferase). [provided by Alliance of Genome Resources, Apr 2025]
Expression
Broad expression in CNS E18 (RPKM 3.8), whole brain E14.5 (RPKM 3.5) and 23 other tissues See more
Orthologs
human all
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Genomic context

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See Alkbh8 in Genome Data Viewer
Location:
9 2.02 cM; 9 A1
Exon count:
14
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 9 NC_000075.7 (3335151..3391154)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 9 NC_000075.6 (3335231..3391154)

Chromosome 9 - NC_000075.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_23119 Neighboring gene predicted gene, 31138 Neighboring gene olfactory receptor family 2 subfamily N member 2, pseudogene 1 Neighboring gene STARR-positive B cell enhancer mm9_chr9:3258734-3259035 Neighboring gene STARR-seq mESC enhancer starr_23120 Neighboring gene STARR-seq mESC enhancer starr_23121 Neighboring gene STARR-positive B cell enhancer ABC_E9751 Neighboring gene predicted gene, 24918 Neighboring gene STARR-seq mESC enhancer starr_23124 Neighboring gene STARR-positive B cell enhancer mm9_chr9:3403367-3403668 Neighboring gene predicted gene, 23275 Neighboring gene CWF19 like cell cycle control factor 2 Neighboring gene STARR-seq mESC enhancer starr_23127

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. ALKBH8 contributes to neurological function through oxidative stress regulation. Honda K, et al. PNAS Nexus, 2024 Mar. PMID 38550277, Free PMC Article
  2. RNA-modifying enzyme Alkbh8 is involved in mouse embryonic development. Nakai M, et al. iScience, 2024 Sep 20. PMID 39280612, Free PMC Article
  3. The epitranscriptomic writer ALKBH8 drives tolerance and protects mouse lungs from the environmental pollutant naphthalene. Leonardi A, et al. Epigenetics, 2020 Oct. PMID 32303148, Free PMC Article
  4. Alkbh8 Regulates Selenocysteine-Protein Expression to Protect against Reactive Oxygen Species Damage. Endres L, et al. PLoS One, 2015. PMID 26147969, Free PMC Article
  5. Loss of epitranscriptomic control of selenocysteine utilization engages senescence and mitochondrial reprogramming(☆). Lee MY, et al. Redox Biol, 2020 Jan. PMID 31765888, Free PMC Article

See all (26) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The epitranscriptomic writer ALKBH8 drives tolerance and protects mouse lungs from the environmental pollutant naphthalene.
    Title: The epitranscriptomic writer ALKBH8 drives tolerance and protects mouse lungs from the environmental pollutant naphthalene.
  2. Loss of epitranscriptomic control of selenocysteine utilization engages senescence and mitochondrial reprogramming().
    Title: Loss of epitranscriptomic control of selenocysteine utilization engages senescence and mitochondrial reprogramming(☆).
  3. Alkbh8 and tRNA modifications as central regulators of cellular oxidative stress responses in mammalian systems.
    Title: Alkbh8 Regulates Selenocysteine-Protein Expression to Protect against Reactive Oxygen Species Damage.
  4. Many eukaryotic tRNAs contain the wobble modification 5-methoxycarbonylmethyl-uridine (mcm5U). It is demonstrated that (R)- and (S)-5-methoxycarbonylhydroxymethyluridine (mchm5U), hydroxylated forms of mcm(5)U, are present in mammalian tRNA-Arg(UCG), and tRNA-Gly(UCC), respectively. It is shown that the hydroxylation reaction leading to the formation of (S)-mchm5U is catalyzed by the oxygenase (AlkB) domain of ALKBH8.
    Title: ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA.
  5. The methyltransferase domain of ALKBH8 is demonstrated to be a functional homologue of the Saccharomyces cerevisiae Trm9 protein, mediating the last step in the formation of the wobble uridine modification 5-methoxycarbonylmethyl-uridine in tRNA. This modification is shown to be important for efficient selenoprotein synthesis. ALKBH8 knock-out mice are generated and described.
    Title: Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding.
  6. The AlkB domain of ALKBH8 specifically hydroxylates mcm(5)U into (S)-mchm(5)U in tRNA-Gly(UCC).
    Title: ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA.
  7. Data show that ALKBH8 is a tRNA methyltransferase required for the final step in the biogenesis of mcm5U.
    Title: Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding.
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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (3) 
  • Gene trapped (1) 
  • Targeted (3)  1 citation

Pathways from PubChem

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General gene information

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Markers

Homology

Clone Names

  • MGC10235

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables 2-oxoglutarate-dependent dioxygenase activity IEA
Inferred from Electronic Annotation
more info
  
enables S-adenosylmethionine-dependent methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables iron ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA (uridine) methyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables tRNA (uridine) methyltransferase activity ISO
Inferred from Sequence Orthology
more info
  
enables tRNA (uridine) methyltransferase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables tRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables tRNA binding ISO
Inferred from Sequence Orthology
more info
  
enables tRNA binding ISS
Inferred from Sequence or Structural Similarity
more info
  
enables zinc ion binding ISO
Inferred from Sequence Orthology
more info
  
enables zinc ion binding ISS
Inferred from Sequence or Structural Similarity
more info
  
Process Evidence Code Pubs
involved_in DNA damage response ISO
Inferred from Sequence Orthology
more info
  
involved_in DNA damage response ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in tRNA methylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tRNA methylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA methylation ISO
Inferred from Sequence Orthology
more info
  
involved_in tRNA methylation ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in tRNA wobble uridine modification IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tRNA wobble uridine modification IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tRNA wobble uridine modification ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISS
Inferred from Sequence or Structural Similarity
more info
  
located_in nucleoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus ISS
Inferred from Sequence or Structural Similarity
more info
  

General protein information

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Preferred Names
tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ALKBH8; alkylated DNA repair protein alkB homolog 8
Names
S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8
S-adenosyl-L-methionine-dependent tRNA methyltransferase ALKBH8
alkB, alkylation repair homolog 8
alpha-ketoglutarate-dependent dioxygenase ALKBH8
probable alpha-ketoglutarate-dependent dioxygenase ABH8
tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8
NP_001391965.1
NP_001391966.1
NP_001391967.1
NP_080579.1
XP_006509944.1
XP_006509946.1
XP_036011094.1
XP_036011095.1
XP_036011096.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001405036.1 → NP_001391965.1  tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ALKBH8 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AC162921

  2. NM_001405037.1 → NP_001391966.1  tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ALKBH8 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC162921
    UniProtKB/Swiss-Prot
    Q3TUG4, Q80Y20, Q8BV08, Q9CX44
    Related
    ENSMUSP00000061511.6, ENSMUST00000053407.13

  3. NM_001405038.1 → NP_001391967.1  tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ALKBH8 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC162921

  4. NM_026303.2 → NP_080579.1  tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ALKBH8 isoform 2

    See identical proteins and their annotated locations for NP_080579.1

    Status: VALIDATED

    Source sequence(s)
    AC162921
    Consensus CDS
    CCDS22792.1
    UniProtKB/Swiss-Prot
    Q3TUG4, Q80Y20, Q8BV08, Q9CX44
    Related
    ENSMUSP00000125996.2, ENSMUST00000165105.2
    Conserved Domains (4) summary
    pfam09004
    Location:1 → 37
    DUF1891; Domain of unknown function (DUF1891)
    cd12431
    Location:42 → 122
    RRM_ALKBH8; RNA recognition motif in alkylated DNA repair protein alkB homolog 8 (ALKBH8) and similar proteins
    pfam13649
    Location:410 → 497
    Methyltransf_25; Methyltransferase domain
    cl21496
    Location:152 → 334
    2OG-FeII_Oxy; 2OG-Fe(II) oxygenase superfamily

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000075.7 Reference GRCm39 C57BL/6J

    Range
    3335151..3391154
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006509883.3 → XP_006509946.1  alkylated DNA repair protein alkB homolog 8 isoform X4

    Conserved Domains (2) summary
    pfam13649
    Location:151 → 238
    Methyltransf_25; Methyltransferase domain
    cl21496
    Location:1 → 44
    2OG-FeII_Oxy; 2OG-Fe(II) oxygenase superfamily

  2. XM_036155201.1 → XP_036011094.1  alkylated DNA repair protein alkB homolog 8 isoform X4

    Conserved Domains (2) summary
    pfam13649
    Location:151 → 238
    Methyltransf_25; Methyltransferase domain
    cl21496
    Location:1 → 44
    2OG-FeII_Oxy; 2OG-Fe(II) oxygenase superfamily

  3. XM_036155203.1 → XP_036011096.1  alkylated DNA repair protein alkB homolog 8 isoform X4

    Conserved Domains (2) summary
    pfam13649
    Location:151 → 238
    Methyltransf_25; Methyltransferase domain
    cl21496
    Location:1 → 44
    2OG-FeII_Oxy; 2OG-Fe(II) oxygenase superfamily

  4. XM_006509881.3 → XP_006509944.1  alkylated DNA repair protein alkB homolog 8 isoform X3

    See identical proteins and their annotated locations for XP_006509944.1

    Conserved Domains (3) summary
    pfam13649
    Location:335 → 422
    Methyltransf_25; Methyltransferase domain
    cl17169
    Location:1 → 47
    RRM_SF; RNA recognition motif (RRM) superfamily
    cl21496
    Location:77 → 259
    2OG-FeII_Oxy; 2OG-Fe(II) oxygenase superfamily

  5. XM_036155202.1 → XP_036011095.1  alkylated DNA repair protein alkB homolog 8 isoform X4

    Conserved Domains (2) summary
    pfam13649
    Location:151 → 238
    Methyltransf_25; Methyltransferase domain
    cl21496
    Location:1 → 44
    2OG-FeII_Oxy; 2OG-Fe(II) oxygenase superfamily
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q80Y20.1 GenPept UniProtKB/Swiss-Prot:Q80Y20

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