Agpat2 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Agpat2provided by MGI
Official Full Name: 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)provided by MGI
Primary source: MGI:MGI:1914762
See related: Ensembl:ENSMUSG00000026922 AllianceGenome:MGI:1914762
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: BSCL; BSCL1; LPAAB; 1-AGPAT 2; LPAAT-beta; 2510002J07Rik
Summary: Enables 1-acylglycerol-3-phosphate O-acyltransferase activity. Predicted to be involved in phosphatidic acid biosynthetic process and positive regulation of cytokine production. Predicted to act upstream of or within lipid metabolic process. Located in endoplasmic reticulum. Is expressed in several structures, including alimentary system; integumental system; nervous system; respiratory system; and urinary system. Used to study congenital generalized lipodystrophy type 1. Human ortholog(s) of this gene implicated in congenital generalized lipodystrophy type 1 and lipodystrophy. Orthologous to human AGPAT2 (1-acylglycerol-3-phosphate O-acyltransferase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in subcutaneous fat pad adult (RPKM 450.2), mammary gland adult (RPKM 425.2) and 15 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2; 2 A3

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (26483069..26494429, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (26593057..26604417, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

AGPAT2 interaction with CDP-diacylglycerol synthases promotes the flux of fatty acids through the CDP-diacylglycerol pathway.
Title: AGPAT2 interaction with CDP-diacylglycerol synthases promotes the flux of fatty acids through the CDP-diacylglycerol pathway.
Adipogenically induced Agpat2(-/-) preadipocytes had fewer lipid-loaded cells and lower levels of adipocyte markers than wild type preadipocytes.
Title: AGPAT2 deficiency impairs adipogenic differentiation in primary cultured preadipocytes in a non-autophagy or apoptosis dependent mechanism.
Leptin ameliorates insulin resistance and hepatic steatosis in Agpat2-/- lipodystrophic mice independent of hepatocyte leptin receptors
Title: Leptin ameliorates insulin resistance and hepatic steatosis in Agpat2-/- lipodystrophic mice independent of hepatocyte leptin receptors.
The lack of normal insulin signaling in Agpat2(-/-) livers allows unrestricted phosphatidic acid-induced gluconeogenesis.
Title: Hepatic gluconeogenesis is enhanced by phosphatidic acid which remains uninhibited by insulin in lipodystrophic Agpat2-/- mice.
Data suggest that Agpat2 regulates adipogenesis through modulation of lipid metabolism/signal transduction, altering normal activation of phosphatidylinositol 3-kinase (PI3K)/protooncogene c-Akt and PPARgamma signaling in early stage of adipogenesis.
Title: Alterations in lipid signaling underlie lipodystrophy secondary to AGPAT2 mutations.
the role of AGPAT1 or AGPAT2 in liver lipogenesis is minimal and that accumulation of liver fat is primarily a consequence of insulin resistance
Title: Human 1-acylglycerol-3-phosphate O-acyltransferase isoforms 1 and 2: biochemical characterization and inability to rescue hepatic steatosis in Agpat2(-/-) gene lipodystrophic mice.
Loss of adipocytes by necrosis and apoptosis in Agpat2 null mice
Title: Pathology of congenital generalized lipodystrophy in Agpat2-/- mice.
The alternative monoacylglycerol pathway for triglyceride biosynthesis is activated in the absence of 1-acylglycerol-3-phosphate O-acyltransferase 2.
Title: Molecular mechanisms of hepatic steatosis and insulin resistance in the AGPAT2-deficient mouse model of congenital generalized lipodystrophy.
There is a prevalence of mutations in this enzymes among human lipodystrophies
Title: Prevalence of mutations in AGPAT2 among human lipodystrophies.
impaired AGPAT2 activity affects availability of phosphatidic acid (PA) for triacylglycerol synthesis but not overall PA synthesis nor utilization of PA for phospholipid synthesis
Title: A regulatory role for 1-acylglycerol-3-phosphate-O-acyltransferase 2 in adipocyte differentiation.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (1) 1 citation
Endonuclease-mediated (3)

General gene information

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Markers

D2Mit417 (e-PCR), detects polymorphism
- UniSTS:129324

Agpat2 (e-PCR), detects polymorphism
- UniSTS:506719

AV000834 (e-PCR)
- UniSTS:178590

NoName (e-PCR)
- UniSTS:491468

Homology

Homologs of the Agpat2 gene: The Agpat2 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, and frog.
Orthologs from Annotation Pipeline: 340 organisms have orthologs with human gene Agpat2
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 1-acylglycerol-3-phosphate O-acyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables 1-acylglycerol-3-phosphate O-acyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables 1-acylglycerol-3-phosphate O-acyltransferase activity	ISO Inferred from Sequence Orthology more info
enables acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidic acid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in phosphatidic acid biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in phospholipid biosynthetic process	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of cytokine production	ISO Inferred from Sequence Orthology more info
involved_in triglyceride biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
is_active_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: 1-acyl-sn-glycerol-3-phosphate acyltransferase beta

Names: 1-AGP acyltransferase 2

NP_080488.1

EC 2.3.1.51

XP_017174711.1

EC 2.3.1.51

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_026212.2 → NP_080488.1 1-acyl-sn-glycerol-3-phosphate acyltransferase beta precursor

See identical proteins and their annotated locations for NP_080488.1

Status: VALIDATED

Source sequence(s)

AK002422, AL732311, BB871777, BC125530

Consensus CDS

CCDS15809.1

UniProtKB/Swiss-Prot

Q8K3K7

UniProtKB/TrEMBL

Q059U0, Q9CWA8

Related

ENSMUSP00000028286.6, ENSMUST00000028286.12

Conserved Domains (2) summary

cd07989
Location:67 → 240

LPLAT_AGPAT-like; Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: AGPAT-like

COG0204
Location:30 → 259

PlsC; 1-acyl-sn-glycerol-3-phosphate acyltransferase [Lipid transport and metabolism]

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

26483069..26494429 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017319222.3 → XP_017174711.1 1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform X1

Related

ENSMUST00000131940.8

Conserved Domains (1) summary

cd07989
Location:4 → 137

LPLAT_AGPAT-like; Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: AGPAT-like