Ctsb cathepsin B [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Ctsbprovided by RGD
Official Full Name: cathepsin Bprovided by RGD
Primary source: RGD:621509
See related: Ensembl:ENSRNOG00000010331 AllianceGenome:RGD:621509
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables several functions, including cysteine-type endopeptidase activity; identical protein binding activity; and kininogen binding activity. Involved in several processes, including cellular response to mechanical stimulus; response to dexamethasone; and response to kainic acid. Located in several cellular components, including caveola; external side of plasma membrane; and sarcolemma. Used to study hepatocellular carcinoma and high grade glioma. Biomarker of several diseases, including acute necrotizing pancreatitis; cerebrovascular disease (multiple); fascioliasis; renal tubular acidosis; and steatotic liver disease (multiple). Human ortholog(s) of this gene implicated in diabetes mellitus. Orthologous to human CTSB (cathepsin B). [provided by Alliance of Genome Resources, Jul 2025]
Expression: Biased expression in Kidney (RPKM 4906.1), Spleen (RPKM 2256.0) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 15p12

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	15	NC_086033.1 (41565607..41586479)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	15	NC_051350.1 (37389636..37410508)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	15	NC_005114.4 (46316741..46337613)

Chromosome 15 - NC_086033.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cystatin C Attenuates Perihematomal Secondary Brain Injury by Inhibiting the Cathepsin B/NLRP3 Signaling Pathway in a Rat Model of Intracerebral Hemorrhage.
Title: Cystatin C Attenuates Perihematomal Secondary Brain Injury by Inhibiting the Cathepsin B/NLRP3 Signaling Pathway in a Rat Model of Intracerebral Hemorrhage.
Chitosan-coated nanostructured lipid carriers for effective brain delivery of Tanshinone IIA in Parkinson's disease: interplay between nuclear factor-kappa beta and cathepsin B.
Title: Chitosan-coated nanostructured lipid carriers for effective brain delivery of Tanshinone IIA in Parkinson's disease: interplay between nuclear factor-kappa β and cathepsin B.
The secretory ability of newly formed secretory granules is regulated by pro-cathepsin B and amylase in parotid glands.
Title: The secretory ability of newly formed secretory granules is regulated by pro-cathepsin B and amylase in parotid glands.
Penetrating Traumatic Brain Injury Triggers Dysregulation of Cathepsin B Protein Levels Independent of Cysteine Protease Activity in Brain and Cerebral Spinal Fluid.
Title: Penetrating Traumatic Brain Injury Triggers Dysregulation of Cathepsin B Protein Levels Independent of Cysteine Protease Activity in Brain and Cerebral Spinal Fluid.
Cathepsin B aggravated doxorubicininduced myocardial injury via NFkappaB signalling.
Title: Cathepsin B aggravated doxorubicin‑induced myocardial injury via NF‑κB signalling.
The results revealed that cathepsin B and L activity is required for maintenance of a fully differentiated state of thyrocytes featuring Taar1-bearing primary cilia.
Title: Treatment of rat thyrocytes in vitro with cathepsin B and L inhibitors results in disruption of primary cilia leading to redistribution of the trace amine associated receptor 1 to the endoplasmic reticulum.
metabolic stress causes an increase in cathepsin B in Wistar rats
Title: Activity of Cathepsin B and Proteome in the Serum and Brain of Rats with Different Behavioral Activity under Conditions of Metabolic Stress.
In untrained animals, cathepsin B activity decreased in liver after one session of exercise. In trained animals, cathepsin B activity in the liver increased immediately after one session of exercise.
Title: One session of exercise regulates cathepsin B activity in the livers of trained and untrained rats.
These results suggest exosomal cathepsin B regulates RAGE in type 1 alveolar cells under conditions of oxidative stress.
Title: Hydrogen Peroxide Stimulates Exosomal Cathepsin B Regulation of the Receptor for Advanced Glycation End-Products (RAGE).
CTSB protein functions as a necessary modulator of hypertrophic response by regulating TNF-alpha/ASK1/JNK signaling pathway involved in cardiac remodeling.
Title: Cathepsin B deficiency attenuates cardiac remodeling in response to pressure overload via TNF-α/ASK1/JNK pathway.

Submit: New GeneRIF Correction See all GeneRIFs (31)

General gene information

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Markers

RH129236 (e-PCR)
- UniSTS:212544

Ctsb (e-PCR), detects polymorphism
- UniSTS:498755

RH130291 (e-PCR)
- UniSTS:213577

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables collagen binding	ISO Inferred from Sequence Orthology more info
enables cysteine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables cysteine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info
enables cysteine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables cysteine-type peptidase activity	IEA Inferred from Electronic Annotation more info
enables cysteine-type peptidase activity	ISO Inferred from Sequence Orthology more info
enables endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables kininogen binding	IPI Inferred from Physical Interaction more info	PubMed
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	ISO Inferred from Sequence Orthology more info
enables peptide binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables proteoglycan binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in autophagy	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to mechanical stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to thyroid hormone stimulus	ISO Inferred from Sequence Orthology more info
involved_in collagen catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within decidualization	ISO Inferred from Sequence Orthology more info
involved_in epithelial cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in neuron apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in proteolysis	IDA Inferred from Direct Assay more info	PubMed
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in proteolysis	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	TAS Traceable Author Statement more info	PubMed
involved_in proteolysis involved in protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis involved in protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in response to amine	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to cytokine	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to dexamethasone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to ethanol	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to glucose	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to interleukin-4	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to kainic acid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to mechanical stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to peptide hormone	IEP Inferred from Expression Pattern more info	PubMed
involved_in skeletal muscle tissue development	IEP Inferred from Expression Pattern more info	PubMed
involved_in spermatogenesis	IEP Inferred from Expression Pattern more info	PubMed
involved_in symbiont entry into host cell	ISO Inferred from Sequence Orthology more info
involved_in thyroid hormone generation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
located_in caveola	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in extracellular region	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IEA Inferred from Electronic Annotation more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular space	ISO Inferred from Sequence Orthology more info
is_active_in lysosome	IBA Inferred from Biological aspect of Ancestor more info
located_in lysosome	IEA Inferred from Electronic Annotation more info
located_in lysosome	ISO Inferred from Sequence Orthology more info
located_in melanosome	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
part_of peptidase inhibitor complex	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in sarcolemma	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: cathepsin B

Names: RSG-2; cathepsin B1

NP_072119.2

EC 3.4.22.1

XP_063130686.1

EC 3.4.22.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_022597.2 → NP_072119.2 cathepsin B preproprotein

See identical proteins and their annotated locations for NP_072119.2

Status: VALIDATED

Source sequence(s)

BC072490, CB697852

UniProtKB/Swiss-Prot

P00787

UniProtKB/TrEMBL

F7FAT8, Q6IN22

Related

ENSRNOP00000014178.4, ENSRNOT00000014177.7

Conserved Domains (2) summary

cd02620
Location:81 → 328

Peptidase_C1A_CathepsinB; Cathepsin B group; composed of cathepsin B and similar proteins, including tubulointerstitial nephritis antigen (TIN-Ag). Cathepsin B is a lysosomal papain-like cysteine peptidase which is expressed in all tissues and functions primarily as an ...

pfam08127
Location:26 → 65

Propeptide_C1; Peptidase family C1 propeptide