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Gpx4 glutathione peroxidase 4 [ Mus musculus (house mouse) ]

Gene ID: 625249, updated on 24-Jan-2023

Summary

Official Symbol
Gpx4provided by MGI
Official Full Name
glutathione peroxidase 4provided by MGI
Primary source
MGI:MGI:104767
See related
Ensembl:ENSMUSG00000075706 AllianceGenome:MGI:104767
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
GPx-4; PHGPx; snGPx; GSHPx-4; mtPHGPx
Summary
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Disruption of this gene in mouse spermatocytes is associated with male infertility. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization. Pseudogenes of this locus have been identified on chromosomes 10 and 17. [provided by RefSeq, Jan 2019]
Expression
Ubiquitous expression in testis adult (RPKM 772.1), kidney adult (RPKM 308.6) and 28 other tissues See more
Orthologs
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Genomic context

See Gpx4 in Genome Data Viewer
Location:
10 C1; 10 39.72 cM
Exon count:
10
Annotation release Status Assembly Chr Location
109 current GRCm39 (GCF_000001635.27) 10 NC_000076.7 (79883000..79892273)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 10 NC_000076.6 (80047166..80056439)

Chromosome 10 - NC_000076.7Genomic Context describing neighboring genes Neighboring gene Rho GTPase activating protein 45 Neighboring gene polymerase (RNA) II (DNA directed) polypeptide E Neighboring gene strawberry notch 2 Neighboring gene serine/threonine kinase 11 Neighboring gene calcium channel, voltage-dependent, beta subunit associated regulatory protein

Genomic regions, transcripts, and products

Expression

  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Variation

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (13)  1 citation
  • Endonuclease-mediated (3) 

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • MGC103187, MGC118087, 1700027O09Rik

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables glutathione peroxidase activity IDA
Inferred from Direct Assay
more info
PubMed 
enables glutathione peroxidase activity ISO
Inferred from Sequence Orthology
more info
PubMed 
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
 
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
 
enables peroxidase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables phospholipid-hydroperoxide glutathione peroxidase activity IDA
Inferred from Direct Assay
more info
PubMed 
enables phospholipid-hydroperoxide glutathione peroxidase activity ISO
Inferred from Sequence Orthology
more info
 
enables selenium binding ISO
Inferred from Sequence Orthology
more info
 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
 
located_in cytosol IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytosol ISO
Inferred from Sequence Orthology
more info
 
located_in mitochondrial inner membrane HDA PubMed 
located_in mitochondrion HDA PubMed 
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
located_in mitochondrion IDA
Inferred from Direct Assay
more info
PubMed 
located_in nuclear envelope IDA
Inferred from Direct Assay
more info
PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
PubMed 
part_of protein-containing complex ISO
Inferred from Sequence Orthology
more info
 

General protein information

Preferred Names
phospholipid hydroperoxide glutathione peroxidase
Names
phospholipid hydroperoxide glutathione peroxidase, mitochondrial
phospholipid hydroperoxide glutathione peroxidase, nuclear
sperm nuclei glutathione peroxidase
NP_001032830.2
NP_001354924.1
NP_032188.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001037741.4NP_001032830.2  phospholipid hydroperoxide glutathione peroxidase isoform B

    See identical proteins and their annotated locations for NP_001032830.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1. The resulting isoform (B) has a longer and a distinct N-terminus compared to isoform A. A similar isoform in rat (snGPx) has been reported to be localized in the sperm nucleus (PMID:11344099).
    Source sequence(s)
    AC159999, AK006441, CX733872
    Consensus CDS
    CCDS24007.1
    UniProtKB/Swiss-Prot
    O70325
    UniProtKB/TrEMBL
    A0A6P5QCS3, Q76LV0
    Related
    ENSMUSP00000094863.5, ENSMUST00000097227.11
    Conserved Domains (1) summary
    cd00340
    Location:96249
    GSH_Peroxidase; Glutathione (GSH) peroxidase family; tetrameric selenoenzymes that catalyze the reduction of a variety of hydroperoxides including lipid peroxidases, using GSH as a specific electron donor substrate. GSH peroxidase contains one selenocysteine residue per ...
  2. NM_001367995.1NP_001354924.1  phospholipid hydroperoxide glutathione peroxidase isoform C

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) uses an alternate promoter, which results in translation initiation from an in-frame downstream AUG start codon compared to variant 1. The encoded isoform (C) has a shorter N-terminus compared to isoform A. A similar isoform in rat (S-form) has been reported to be localized to the cytoplasm (PMID:9988735).
    Source sequence(s)
    AK006441, CX733872
    UniProtKB/TrEMBL
    Q3TI34
    Related
    ENSMUSP00000138186.3, ENSMUST00000183037.3
  3. NM_008162.4NP_032188.3  phospholipid hydroperoxide glutathione peroxidase isoform A precursor

    See identical proteins and their annotated locations for NP_032188.3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the predominant transcript and encodes the canonical isoform A (also known as phGPx). A similar isoform in rat(L-form) has been shown to be localized to the mitochondria (PMID:9988735).
    Source sequence(s)
    AK006441, BY238083, CX733872
    Consensus CDS
    CCDS35973.1
    UniProtKB/Swiss-Prot
    O70325, Q9JK35
    UniProtKB/TrEMBL
    A0A6P5QJE1
    Related
    ENSMUSP00000101011.3, ENSMUST00000105372.9
    Conserved Domains (1) summary
    cd00340
    Location:40193
    GSH_Peroxidase; Glutathione (GSH) peroxidase family; tetrameric selenoenzymes that catalyze the reduction of a variety of hydroperoxides including lipid peroxidases, using GSH as a specific electron donor substrate. GSH peroxidase contains one selenocysteine residue per ...

RNA

  1. NR_110342.1 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) contains alternate 5' terminal exons and lacks an internal exon compared to variant 1. This variant is represented as non-coding because the predicted protein does not meet RefSeq quality criteria.
    Source sequence(s)
    AA510431, AK145770
    Related
    ENSMUST00000144698.8

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000076.7 Reference GRCm39 C57BL/6J

    Range
    79883000..79892273
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)