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RPA2 replication protein A2 [ Homo sapiens (human) ]

Gene ID: 6118, updated on 13-Mar-2020

Summary

Official Symbol
RPA2provided by HGNC
Official Full Name
replication protein A2provided by HGNC
Primary source
HGNC:HGNC:10290
See related
Ensembl:ENSG00000117748 MIM:179836
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
REPA2; RPA32; RP-A p32; RP-A p34
Summary
This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]
Expression
Ubiquitous expression in testis (RPKM 29.0), lymph node (RPKM 26.6) and 25 other tissues See more
Orthologs

Genomic context

See RPA2 in Genome Data Viewer
Location:
1p35.3
Exon count:
9
Annotation release Status Assembly Chr Location
109.20200228 current GRCh38.p13 (GCF_000001405.39) 1 NC_000001.11 (27891524..27914797, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (28218035..28241255, complement)

Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene CRISPRi-validated cis-regulatory element chr1.3986 Neighboring gene thymocyte selection associated family member 2 Neighboring gene sphingomyelin phosphodiesterase acid like 3B Neighboring gene XK related 8

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
HIV-1 replication is enhanced by RPA2 in monocyte derived macrophages PubMed
HIV-1 replication is enhanced by RPA2 in PBMCs by influencing the efficiency of reverse transcription PubMed
HIV-1 replication is enhanced by RPA2 (HeLa-CD4 cells and Jurkat T cells) as shown by shRNA-mediated knockdown of RPA2 PubMed
Depletion of RPA2 by shRNA in HIV-1-producing 293T cells inhibits viral infectivity and replication PubMed

Protein interactions

Protein Gene Interaction Pubs
Vif vif HIV-1 Vif-induced G2 delay is inhibited by caffeine and correlates with the activation of RPA-32 protein PubMed
Vpr vpr HIV-1 Vpr complexes with RPA2 via UNG2 to form a trimolecular complex (containing Vpr, UNG2 and RPA2) PubMed
vpr HIV-1 Vpr forms nuclear foci containing VPRBP and partially co-localizes with DNA repair foci components 53BP1 and phosphorylated RPA32 PubMed
vpr HIV-1 Vpr induces phosphorylation of replication protein A (RPA2) at serine 4 and 8 in an ATR-dependent manner PubMed
reverse transcriptase gag-pol Replication protein A and HIV-1 nucleocapsid protein interfere with the strand displacement DNA synthesis of HIV-1 reverse transcriptase by binding to the displaced strand and keeping it away from the newly synthesized strand PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
G-rich strand telomeric DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
damaged DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
enzyme binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein N-terminus binding IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein phosphatase binding IPI
Inferred from Physical Interaction
more info
PubMed 
single-stranded DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
single-stranded DNA binding IC
Inferred by Curator
more info
PubMed 
single-stranded DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
single-stranded DNA binding ISS
Inferred from Sequence or Structural Similarity
more info
 
ubiquitin protein ligase binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
DNA damage response, detection of DNA damage TAS
Traceable Author Statement
more info
 
DNA repair IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
DNA replication IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
DNA replication IDA
Inferred from Direct Assay
more info
PubMed 
DNA replication IMP
Inferred from Mutant Phenotype
more info
PubMed 
DNA replication TAS
Traceable Author Statement
more info
 
G1/S transition of mitotic cell cycle TAS
Traceable Author Statement
more info
 
base-excision repair IDA
Inferred from Direct Assay
more info
PubMed 
double-strand break repair via homologous recombination IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
double-strand break repair via homologous recombination IMP
Inferred from Mutant Phenotype
more info
PubMed 
error-free translesion synthesis TAS
Traceable Author Statement
more info
 
error-prone translesion synthesis TAS
Traceable Author Statement
more info
 
interstrand cross-link repair TAS
Traceable Author Statement
more info
 
mismatch repair IMP
Inferred from Mutant Phenotype
more info
PubMed 
mitotic G1 DNA damage checkpoint IMP
Inferred from Mutant Phenotype
more info
PubMed 
nucleotide-excision repair IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
nucleotide-excision repair IMP
Inferred from Mutant Phenotype
more info
PubMed 
nucleotide-excision repair, DNA gap filling TAS
Traceable Author Statement
more info
 
nucleotide-excision repair, DNA incision TAS
Traceable Author Statement
more info
 
nucleotide-excision repair, DNA incision, 3'-to lesion TAS
Traceable Author Statement
more info
 
nucleotide-excision repair, DNA incision, 5'-to lesion TAS
Traceable Author Statement
more info
 
nucleotide-excision repair, preincision complex assembly TAS
Traceable Author Statement
more info
 
nucleotide-excision repair, preincision complex stabilization TAS
Traceable Author Statement
more info
 
protein localization to chromosome IDA
Inferred from Direct Assay
more info
PubMed 
regulation of DNA damage checkpoint IMP
Inferred from Mutant Phenotype
more info
PubMed 
regulation of cellular response to heat TAS
Traceable Author Statement
more info
 
regulation of double-strand break repair via homologous recombination IMP
Inferred from Mutant Phenotype
more info
PubMed 
regulation of signal transduction by p53 class mediator TAS
Traceable Author Statement
more info
 
telomere maintenance IC
Inferred by Curator
more info
PubMed 
telomere maintenance IMP
Inferred from Mutant Phenotype
more info
PubMed 
telomere maintenance via semi-conservative replication TAS
Traceable Author Statement
more info
 
transcription-coupled nucleotide-excision repair TAS
Traceable Author Statement
more info
 
translesion synthesis TAS
Traceable Author Statement
more info
 
Component Evidence Code Pubs
DNA replication factor A complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
DNA replication factor A complex IDA
Inferred from Direct Assay
more info
PubMed 
DNA replication factor A complex IPI
Inferred from Physical Interaction
more info
PubMed 
PML body IDA
Inferred from Direct Assay
more info
PubMed 
colocalizes_with PML body IDA
Inferred from Direct Assay
more info
PubMed 
chromatin IDA
Inferred from Direct Assay
more info
PubMed 
colocalizes_with chromosome, telomeric region IDA
Inferred from Direct Assay
more info
PubMed 
nuclear body IDA
Inferred from Direct Assay
more info
 
nuclear chromosome, telomeric region HDA PubMed 
nucleoplasm IDA
Inferred from Direct Assay
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
colocalizes_with site of double-strand break IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
replication protein A 32 kDa subunit
Names
RF-A protein 2
replication factor A protein 2
replication protein A 34 kDa subunit

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001286076.1NP_001273005.1  replication protein A 32 kDa subunit isoform 2

    See identical proteins and their annotated locations for NP_001273005.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1.
    Source sequence(s)
    AK300691, AL109927, BC001630, BG107669, DB025488
    UniProtKB/Swiss-Prot
    P15927
    UniProtKB/TrEMBL
    B4DUL2
    Conserved Domains (2) summary
    cd04478
    Location:471
    RPA2_DBD_D; RPA2_DBD_D: A subfamily of OB folds corresponding to the OB fold of the central ssDNA-binding domain (DBD)-D of human RPA2 (also called RPA32). RPA2 is a subunit of Replication protein A (RPA). RPA is a nuclear ssDNA-binding protein (SSB) which appears ...
    pfam08784
    Location:70166
    RPA_C; Replication protein A C terminal
  2. NM_001297558.1NP_001284487.1  replication protein A 32 kDa subunit isoform 3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) uses an alternate splice site in the 5' region and initiates translation at an alternate upstream start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is longer than isoform 1.
    Source sequence(s)
    BC001630, BQ006360, BX333932, DB025488
    Consensus CDS
    CCDS72740.1
    UniProtKB/Swiss-Prot
    P15927
    Related
    ENSP00000363017.3, ENST00000373909.7
    Conserved Domains (3) summary
    COG5235
    Location:33275
    RFA2; Single-stranded DNA-binding replication protein A (RPA), medium (30 kD) subunit [DNA replication, recombination, and repair]
    cd04478
    Location:81175
    RPA2_DBD_D; RPA2_DBD_D: A subfamily of OB folds corresponding to the OB fold of the central ssDNA-binding domain (DBD)-D of human RPA2 (also called RPA32). RPA2 is a subunit of Replication protein A (RPA). RPA is a nuclear ssDNA-binding protein (SSB) which appears ...
    pfam08784
    Location:174270
    RPA_C; Replication protein A C terminal
  3. NM_001355128.1NP_001342057.1  replication protein A 32 kDa subunit isoform 2

    Status: REVIEWED

    Source sequence(s)
    BC001630, BG107669, BM839986, DB025488
    Conserved Domains (2) summary
    cd04478
    Location:471
    RPA2_DBD_D; RPA2_DBD_D: A subfamily of OB folds corresponding to the OB fold of the central ssDNA-binding domain (DBD)-D of human RPA2 (also called RPA32). RPA2 is a subunit of Replication protein A (RPA). RPA is a nuclear ssDNA-binding protein (SSB) which appears ...
    pfam08784
    Location:70166
    RPA_C; Replication protein A C terminal
  4. NM_001355129.1NP_001342058.1  replication protein A 32 kDa subunit isoform 4

    Status: REVIEWED

    Source sequence(s)
    BC001630, BG107669, CB155053, DB025488
    Conserved Domains (2) summary
    cd04478
    Location:77171
    RPA2_DBD_D; A subfamily of OB folds corresponding to the OB fold of the central ssDNA-binding domain (DBD)-D of human RPA2 (also called RPA32). RPA2 is a subunit of Replication protein A (RPA). RPA is a nuclear ssDNA-binding protein (SSB) which appears to be ...
    cl27871
    Location:29271
    RPA_C; Replication protein A C terminal
  5. NM_002946.5NP_002937.1  replication protein A 32 kDa subunit isoform 1

    See identical proteins and their annotated locations for NP_002937.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes isoform 1.
    Source sequence(s)
    BC001630, BG107669, DB025488
    Consensus CDS
    CCDS314.1
    UniProtKB/Swiss-Prot
    P15927
    Related
    ENSP00000363021.3, ENST00000373912.8
    Conserved Domains (2) summary
    cd04478
    Location:73167
    RPA2_DBD_D; RPA2_DBD_D: A subfamily of OB folds corresponding to the OB fold of the central ssDNA-binding domain (DBD)-D of human RPA2 (also called RPA32). RPA2 is a subunit of Replication protein A (RPA). RPA is a nuclear ssDNA-binding protein (SSB) which appears ...
    cl27871
    Location:25267
    RPA_C; Replication protein A C terminal

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000001.11 Reference GRCh38.p13 Primary Assembly

    Range
    27891524..27914797 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_024448864.1XP_024304632.1  replication protein A 32 kDa subunit isoform X2

    Conserved Domains (2) summary
    cd04478
    Location:73142
    RPA2_DBD_D; A subfamily of OB folds corresponding to the OB fold of the central ssDNA-binding domain (DBD)-D of human RPA2 (also called RPA32). RPA2 is a subunit of Replication protein A (RPA). RPA is a nuclear ssDNA-binding protein (SSB) which appears to be ...
    pfam08784
    Location:141237
    RPA_C; Replication protein A C terminal
  2. XM_024448862.1XP_024304630.1  replication protein A 32 kDa subunit isoform X1

    Conserved Domains (2) summary
    cd04478
    Location:81150
    RPA2_DBD_D; A subfamily of OB folds corresponding to the OB fold of the central ssDNA-binding domain (DBD)-D of human RPA2 (also called RPA32). RPA2 is a subunit of Replication protein A (RPA). RPA is a nuclear ssDNA-binding protein (SSB) which appears to be ...
    pfam08784
    Location:149245
    RPA_C; Replication protein A C terminal
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