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RPA1 replication protein A1 [ Homo sapiens (human) ]

Gene ID: 6117, updated on 5-Apr-2020

Summary

Official Symbol
RPA1provided by HGNC
Official Full Name
replication protein A1provided by HGNC
Primary source
HGNC:HGNC:10289
See related
Ensembl:ENSG00000132383 MIM:179835
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
HSSB; RF-A; RP-A; REPA1; RPA70; MST075
Summary
This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways. [provided by RefSeq, Sep 2017]
Expression
Ubiquitous expression in lymph node (RPKM 21.6), ovary (RPKM 19.7) and 25 other tissues See more
Orthologs

Genomic context

See RPA1 in Genome Data Viewer
Location:
17p13.3
Exon count:
17
Annotation release Status Assembly Chr Location
109.20200228 current GRCh38.p13 (GCF_000001405.39) 17 NC_000017.11 (1830005..1900082)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (1733273..1802848)

Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene serpin family F member 1 Neighboring gene Sharpr-MPRA regulatory region 4981 Neighboring gene SET and MYND domain containing 4 Neighboring gene succinate dehydrogenase complex subunit C pseudogene Neighboring gene reticulon 4 receptor like 1 Neighboring gene uncharacterized LOC105371486 Neighboring gene uncharacterized LOC105371485 Neighboring gene diphthamide biosynthesis 1 Neighboring gene OVCA2 serine hydrolase domain containing

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Tat tat HIV-1 Tat interacts with the RNA polymerase II holoenzyme, which includes RPA, during Tat-mediated transactivation of the HIV-1 LTR PubMed
Vpr vpr HIV-1 Vpr-induced activation of ATR increases the chromatin binding of replication protein A (RPA) PubMed
reverse transcriptase gag-pol Replication protein A and HIV-1 nucleocapsid protein interfere with the strand displacement DNA synthesis of HIV-1 reverse transcriptase by binding to the displaced strand and keeping it away from the newly synthesized strand PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
G-rich strand telomeric DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
G-rich strand telomeric DNA binding IMP
Inferred from Mutant Phenotype
more info
PubMed 
damaged DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
damaged DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
metal ion binding IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
single-stranded DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
single-stranded DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
single-stranded telomeric DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Process Evidence Code Pubs
DNA damage response, detection of DNA damage TAS
Traceable Author Statement
more info
 
DNA recombination TAS
Traceable Author Statement
more info
PubMed 
DNA repair IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
DNA repair IMP
Inferred from Mutant Phenotype
more info
PubMed 
DNA replication IMP
Inferred from Mutant Phenotype
more info
PubMed 
DNA replication TAS
Traceable Author Statement
more info
 
DNA unwinding involved in DNA replication IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
DNA-dependent DNA replication IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
G1/S transition of mitotic cell cycle TAS
Traceable Author Statement
more info
 
base-excision repair IDA
Inferred from Direct Assay
more info
PubMed 
cellular response to DNA damage stimulus IMP
Inferred from Mutant Phenotype
more info
PubMed 
double-strand break repair via homologous recombination IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
double-strand break repair via homologous recombination IMP
Inferred from Mutant Phenotype
more info
PubMed 
error-free translesion synthesis TAS
Traceable Author Statement
more info
 
error-prone translesion synthesis TAS
Traceable Author Statement
more info
 
interstrand cross-link repair TAS
Traceable Author Statement
more info
 
meiotic cell cycle IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
mismatch repair IMP
Inferred from Mutant Phenotype
more info
PubMed 
nucleotide-excision repair IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
nucleotide-excision repair IMP
Inferred from Mutant Phenotype
more info
PubMed 
nucleotide-excision repair, DNA gap filling TAS
Traceable Author Statement
more info
 
nucleotide-excision repair, DNA incision TAS
Traceable Author Statement
more info
 
nucleotide-excision repair, DNA incision, 3'-to lesion TAS
Traceable Author Statement
more info
 
nucleotide-excision repair, DNA incision, 5'-to lesion TAS
Traceable Author Statement
more info
 
nucleotide-excision repair, preincision complex assembly TAS
Traceable Author Statement
more info
 
nucleotide-excision repair, preincision complex stabilization TAS
Traceable Author Statement
more info
 
protein localization to chromosome IDA
Inferred from Direct Assay
more info
PubMed 
regulation of cellular response to heat TAS
Traceable Author Statement
more info
 
regulation of signal transduction by p53 class mediator TAS
Traceable Author Statement
more info
 
telomere maintenance IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
telomere maintenance IC
Inferred by Curator
more info
PubMed 
telomere maintenance IMP
Inferred from Mutant Phenotype
more info
PubMed 
telomere maintenance via semi-conservative replication TAS
Traceable Author Statement
more info
 
telomere maintenance via telomerase IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
transcription-coupled nucleotide-excision repair TAS
Traceable Author Statement
more info
 
translesion synthesis TAS
Traceable Author Statement
more info
 
Component Evidence Code Pubs
DNA replication factor A complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
DNA replication factor A complex IDA
Inferred from Direct Assay
more info
PubMed 
DNA replication factor A complex IPI
Inferred from Physical Interaction
more info
PubMed 
PML body IDA
Inferred from Direct Assay
more info
PubMed 
colocalizes_with PML body IDA
Inferred from Direct Assay
more info
PubMed 
nuclear chromosome, telomeric region HDA PubMed 
nuclear chromosome, telomeric region IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
nucleoplasm IDA
Inferred from Direct Assay
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
site of DNA damage IMP
Inferred from Mutant Phenotype
more info
PubMed 

General protein information

Preferred Names
replication protein A 70 kDa DNA-binding subunit
Names
MSTP075
RF-A protein 1
RP-A p70
replication factor A protein 1
replication protein A1, 70kDa
single-stranded DNA-binding protein

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001355120.2NP_001342049.1  replication protein A 70 kDa DNA-binding subunit isoform 2

    Status: REVIEWED

    Source sequence(s)
    AC130689, AW966691, BC018126, BM666146, BP364363, BP432249, BQ004759, BX110398, M63488, R56356
    Conserved Domains (1) summary
    TIGR00617
    Location:1599
    rpa1; replication factor-a protein 1 (rpa1)
  2. NM_001355121.2NP_001342050.1  replication protein A 70 kDa DNA-binding subunit isoform 3

    Status: REVIEWED

    Source sequence(s)
    AC130689, AW966691, BC018126, BM666146, BP432249, BQ004759, BX110398, CX871238, M63488, R56356
    Conserved Domains (1) summary
    cl28407
    Location:2553
    RFA1; ssDNA-binding replication factor A, large subunit [Replication, recombination and repair]
  3. NM_002945.5NP_002936.1  replication protein A 70 kDa DNA-binding subunit isoform 1

    See identical proteins and their annotated locations for NP_002936.1

    Status: REVIEWED

    Source sequence(s)
    AC130689, AW966691, BC018126, BM666146, BP432249, BQ004759, BX110398, M63488, R56356
    Consensus CDS
    CCDS11014.1
    UniProtKB/Swiss-Prot
    P27694
    Related
    ENSP00000254719.4, ENST00000254719.10
    Conserved Domains (5) summary
    cd04474
    Location:186288
    RPA1_DBD_A; RPA1_DBD_A: A subfamily of OB folds corresponding to the second OB fold, the ssDNA-binding domain (DBD)-A, of human RPA1 (also called RPA70). RPA1 is the large subunit of Replication protein A (RPA). RPA is a nuclear ssDNA-binding protein (SSB) which ...
    TIGR00617
    Location:2612
    rpa1; replication factor-a protein 1 (rpa1)
    pfam04057
    Location:5104
    Rep-A_N; Replication factor-A protein 1, N-terminal domain
    pfam08646
    Location:461606
    Rep_fac-A_C; Replication factor-A C terminal domain
    pfam16900
    Location:305402
    REPA_OB_2; Replication protein A OB domain

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000017.11 Reference GRCh38.p13 Primary Assembly

    Range
    1830005..1900082
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_024450863.1XP_024306631.1  replication protein A 70 kDa DNA-binding subunit isoform X1

    Conserved Domains (1) summary
    TIGR00617
    Location:48614
    rpa1; replication factor-a protein 1 (rpa1)
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