Nsf N-ethylmaleimide sensitive factor, vesicle fusing ATPase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Nsfprovided by RGD
Official Full Name: N-ethylmaleimide sensitive factor, vesicle fusing ATPaseprovided by RGD
Primary source: RGD:621594
See related: Ensembl:ENSRNOG00000003905 AllianceGenome:RGD:621594
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables several functions, including ATP hydrolysis activity; enzyme binding activity; and signaling receptor binding activity. Involved in protein-containing complex disassembly; regulation of vesicle-mediated transport; and vesicle-mediated transport. Located in Golgi stack and dendritic shaft. Is active in GABA-ergic synapse and postsynaptic specialization, intracellular component. Biomarker of temporal lobe epilepsy. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 96. Orthologous to human NSF (N-ethylmaleimide sensitive factor, vesicle fusing ATPase). [provided by Alliance of Genome Resources, Jul 2025]
Expression: Biased expression in Brain (RPKM 771.6), Adrenal (RPKM 258.6) and 6 other tissues See more
Orthologs: all
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Genomic context

Location:: 10q32.1

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (89227937..89357383, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (88727912..88857375, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (91879608..91986242, complement)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Once trans-SNARE complexes that form large, stable pores by Ca2+-synaptotagmin 1, they can only be closed by the action of the N-ethylmaleimide sensitive factor(NSF). Time-resolved measurements reveal that NSF-mediated pore closure occurs via a complex 'stuttering' mechanism.
Title: Resolving kinetic intermediates during the regulated assembly and disassembly of fusion pores.
Results indicate that glucocorticoid hormones increase AMPA receptor mediated synaptic transmission and synaptic insertion of AMPA receptors via a mechanism that requires NSF/GluA2 interaction. This may contribute to the memory enhancing effects of glucocorticoid hormones.
Title: Interactions between N-Ethylmaleimide-sensitive factor and GluA2 contribute to effects of glucocorticoid hormones on AMPA receptor function in the rodent hippocampus.
We have found increased expression of NSF gene in basolateral amygdala and perirhinal cortex in groups of rats undergoing taste habituation.
Title: Increased N-Ethylmaleimide-Sensitive Factor Expression in Amygdala and Perirhinal Cortex during Habituation of Taste Neophobia.
Results indicate that the persistence of long-term memory depends on the maintenance of a steady-state level of synaptic GluA2/AMPARs, which requires the interaction of NSF with GluA2
Title: The maintenance of long-term memory in the hippocampus depends on the interaction between N-ethylmaleimide-sensitive factor and GluA2.
TCST increases the expression of NSF in the hippocampus of adult rats with focal cerebral ischemia, which may contribute to the protection of the injured brain.
Title: Identification of differentially expressed proteins and validation of the changes of N-ethylmaleimide-sensitive factor in rats with focal cerebral ischemia after transection of the cervical sympathetic trunk.
NSF-GluR2 interactions increase in the nucleus accumbens after cocaine withdrawal.
Title: Interactions between N-ethylmaleimide-sensitive factor and GluR2 in the nucleus accumbens contribute to the expression of locomotor sensitization to cocaine.
a model of NSF-mediated disassembly in which the reaction is initiated by a 1:1 interaction between alpha-SNAP and the ternary SNARE complex, followed by NSF binding.
Title: Disassembly of all SNARE complexes by N-ethylmaleimide-sensitive factor (NSF) is initiated by a conserved 1:1 interaction between α-soluble NSF attachment protein (SNAP) and SNARE complex.
The interaction of N-ethylmaleimide-sensitive factor (NSF) with glutamate receptor 2 (GluR2) is necessary for fear memory consolidation.
Title: Interaction between N-ethylmaleimide-sensitive factor and GluR2 is essential for fear memory formation in lateral amygdala.
Concomitant with the decrease in NSF, GluR2 and GluR3 were decreased and GluR1 was conversely increased in the postsynaptic density fraction 24 h after complete Freund adjuvant injection
Title: N-ethylmaleimide-sensitive fusion protein (NSF) is involved in central sensitization in the spinal cord through GluR2 subunit composition switch after inflammation.
Synaptic stabilization of AMPA receptors by NSF involves disruption of GluR2-PICK1 interactions.
Title: NSF ATPase and alpha-/beta-SNAPs disassemble the AMPA receptor-PICK1 complex.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Nsf (e-PCR), detects polymorphism
- UniSTS:497472

RH134933 (e-PCR)
- UniSTS:218210

NSF-1 (e-PCR)
- UniSTS:512128

AU049932 (e-PCR)
- UniSTS:146374

AW531537 (e-PCR)
- UniSTS:261835

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	ISO Inferred from Sequence Orthology more info
enables ATP-dependent protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ATP-dependent protein disaggregase activity	ISS Inferred from Sequence or Structural Similarity more info
enables D1 dopamine receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables PDZ domain binding	ISO Inferred from Sequence Orthology more info
enables SNARE binding	ISS Inferred from Sequence or Structural Similarity more info
enables calcium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ionotropic glutamate receptor binding	IDA Inferred from Direct Assay more info	PubMed
enables ionotropic glutamate receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables ionotropic glutamate receptor binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	ISS Inferred from Sequence or Structural Similarity more info
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi to plasma membrane protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in SNARE complex disassembly	IEA Inferred from Electronic Annotation more info
involved_in SNARE complex disassembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in intra-Golgi vesicle-mediated transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular protein transport	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of AMPA glutamate receptor clustering	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of excitatory postsynaptic potential	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of filopodium assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of receptor internalization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of receptor recycling	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of receptor-mediated endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within potassium ion transport	ISO Inferred from Sequence Orthology more info
involved_in protein homooligomerization	IDA Inferred from Direct Assay more info	PubMed
involved_in protein-containing complex disassembly	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of receptor internalization	IDA Inferred from Direct Assay more info	PubMed
involved_in response to hyperoxia	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to morphine	IEP Inferred from Expression Pattern more info	PubMed
involved_in surfactant secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle-mediated transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in GABA-ergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in Golgi stack	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi stack	IDA Inferred from Direct Assay more info	PubMed
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in axon terminus	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in dendritic filopodium	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic shaft	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine neck	IDA Inferred from Direct Assay more info	PubMed
located_in lamellar body	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
located_in postsynaptic density	TAS Traceable Author Statement more info	PubMed
is_active_in postsynaptic specialization, intracellular component	IDA Inferred from Direct Assay more info	PubMed
located_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: vesicle-fusing ATPase

Names: N-ethylmaleimide-sensitive factor; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; vesicular-fusion protein NSF

NP_068516.2

EC 3.6.4.6

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_021748.2 → NP_068516.2 vesicle-fusing ATPase

Status: VALIDATED

Source sequence(s)

JAXUCZ010000010

UniProtKB/Swiss-Prot

Q9QUL6

UniProtKB/TrEMBL

A6HJT3, F1LQ81

Related

ENSRNOP00000006361.6, ENSRNOT00000006361.8

Conserved Domains (4) summary

smart01072
Location:111 → 183

CDC48_2; Cell division protein 48 (CDC48) domain 2

smart01073
Location:6 → 83

CDC48_N; Cell division protein 48 (CDC48) N-terminal domain

TIGR01243
Location:159 → 606

CDC48; AAA family ATPase, CDC48 subfamily

cd19504
Location:219 → 395

RecA-like_NSF-SEC18_r1-like; first of two ATPase domains of NSF and SEC18, and similar ATPase domains