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PSMD3 proteasome 26S subunit, non-ATPase 3 [ Homo sapiens (human) ]

Gene ID: 5709, updated on 7-Sep-2023


Official Symbol
PSMD3provided by HGNC
Official Full Name
proteasome 26S subunit, non-ATPase 3provided by HGNC
Primary source
See related
Ensembl:ENSG00000108344 MIM:617676; AllianceGenome:HGNC:9560
Gene type
protein coding
RefSeq status
Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
S3; P58; RPN3; TSTA2
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid. Single nucleotide polymorphisms in this gene are associated with neutrophil count. [provided by RefSeq, Jul 2012]
Ubiquitous expression in colon (RPKM 31.7), brain (RPKM 31.3) and 25 other tissues See more
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Genomic context

See PSMD3 in Genome Data Viewer
Exon count:
Annotation release Status Assembly Chr Location
RS_2023_03 current GRCh38.p14 (GCF_000001405.40) 17 NC_000017.11 (39980807..39997959)
RS_2023_03 current T2T-CHM13v2.0 (GCF_009914755.1) 17 NC_060941.1 (40844670..40861847)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (38137060..38154212)

Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr17:38083719-38084330 Neighboring gene leucine rich repeat containing 3C Neighboring gene gasdermin A Neighboring gene P300/CBP strongly-dependent group 1 enhancer GRCh37_chr17:38134870-38136069 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr17:38136971-38137948 Neighboring gene uncharacterized LOC124904000 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr17:38167559-38168498 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr17:38171603-38172102 Neighboring gene colony stimulating factor 3 Neighboring gene mediator complex subunit 24 Neighboring gene microRNA 6884

Genomic regions, transcripts, and products


  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018


GeneRIFs: Gene References Into Functions

What's a GeneRIF?


EBI GWAS Catalog

Common variations in PSMD3-CSF3 and PLCB4 are associated with neutrophil count.
EBI GWAS Catalog
Genetic variants associated with the white blood cell count in 13,923 subjects in the eMERGE Network.
EBI GWAS Catalog
Genome-wide association study of hematological and biochemical traits in a Japanese population.
EBI GWAS Catalog
Identification of nine novel loci associated with white blood cell subtypes in a Japanese population.
EBI GWAS Catalog
Multiple loci are associated with white blood cell phenotypes.
EBI GWAS Catalog

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Tat tat HIV-1 Tat slightly enhances the activity of the purified 26 S proteasome PubMed
tat Amino acids Lys51, Arg52, and Asp67 of HIV-1 Tat represent the proteasome binding site of Tat, and Tat amino acids 37-72 are necessary for proteasomal interaction and suppression of 11 S regulator-mediated antigen presentation PubMed
tat HIV-1 Tat inhibits the peptidase activity of the 20 S proteasome and interferes with the formation of the 20 S proteasome-11 S regulator complex PubMed
tat HIV-1 Tat binds to the alpha2, alpha4, alpha6, alpha7, beta1, beta2, beta3, beta5, beta6, beta7, LMP7/beta5i, and MECL1/beta2i subunits of the proteasome 20 S core structure and can inhibit cellular proteasome function PubMed
Vif vif HIV-1 Vif binds to the cellular cytidine deaminase APOBEC3G and targets it for degradation through an interaction with the proteasome, thereby inhibiting APOBEC3G mediated restriction of HIV-1 replication PubMed
integrase gag-pol Proteasomal degradation of HIV-1 integrase in mammalian cells occurs by the N-end rule pathway PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem


Products Interactant Other Gene Complex Source Pubs Description

General gene information


Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables enzyme regulator activity IEA
Inferred from Electronic Annotation
more info
enables protein binding IPI
Inferred from Physical Interaction
more info
Component Evidence Code Pubs
located_in cytosol TAS
Traceable Author Statement
more info
located_in extracellular exosome HDA PubMed 
located_in extracellular region TAS
Traceable Author Statement
more info
located_in ficolin-1-rich granule lumen TAS
Traceable Author Statement
more info
located_in membrane HDA PubMed 
located_in nucleoplasm TAS
Traceable Author Statement
more info
located_in nucleus HDA PubMed 
part_of proteasome accessory complex ISS
Inferred from Sequence or Structural Similarity
more info
part_of proteasome complex IDA
Inferred from Direct Assay
more info
part_of proteasome complex NAS
Non-traceable Author Statement
more info
part_of proteasome regulatory particle, lid subcomplex IBA
Inferred from Biological aspect of Ancestor
more info
located_in secretory granule lumen TAS
Traceable Author Statement
more info

General protein information

Preferred Names
26S proteasome non-ATPase regulatory subunit 3
26S proteasome regulatory subunit RPN3
26S proteasome regulatory subunit S3
proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
proteasome subunit p58
tissue specific transplantation antigen 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_002809.4NP_002800.2  26S proteasome non-ATPase regulatory subunit 3

    See identical proteins and their annotated locations for NP_002800.2

    Status: REVIEWED

    Source sequence(s)
    AA143716, D67025, HY034626
    Consensus CDS
    B3KMW9, B4DT72, O43242, Q96EI2, Q9BQA4
    ENSP00000264639.4, ENST00000264639.9
    Conserved Domains (2) summary
    PCI; PCI domain
    Rpn3_C; Proteasome regulatory subunit C-terminal

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_03

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly


  1. NC_000017.11 Reference GRCh38.p14 Primary Assembly

    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0


  1. NC_060941.1 Alternate T2T-CHM13v2.0

    GenBank, FASTA, Sequence Viewer (Graphics)