Ripk3 receptor-interacting serine-threonine kinase 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ripk3provided by MGI
Official Full Name: receptor-interacting serine-threonine kinase 3provided by MGI
Primary source: MGI:MGI:2154952
See related: Ensembl:ENSMUSG00000022221 AllianceGenome:MGI:2154952
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Rip3; 2610528K09Rik
Summary: Enables protein serine/threonine kinase activity. Involved in several processes, including execution phase of necroptosis; hematopoietic or lymphoid organ development; and positive regulation of necroptotic process. Acts upstream of or within with a positive effect on non-canonical NF-kappaB signal transduction. Acts upstream of or within several processes, including positive regulation of intrinsic apoptotic signaling pathway; positive regulation of ligase activity; and positive regulation of oxidoreductase activity. Located in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; central nervous system; immune system; male reproductive gland or organ; and turbinate bone primordium. Orthologous to human RIPK3 (receptor interacting serine/threonine kinase 3). [provided by Alliance of Genome Resources, Jul 2025]
Annotation information: Note: Mprip (Gene ID: 26936) and Ripk3 (Gene ID: 56532) share the RIP3 symbol/alias in common. RIP3 is a widely used alternative name for Ripk3 (Gene ID: 56532), which can be confused with Mprip (Gene ID: 26936), which also uses RIP3 as an alternative name. [05 Mar 2019]
Expression: Broad expression in large intestine adult (RPKM 17.7), thymus adult (RPKM 10.3) and 18 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 C3; 14 28.19 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (56022452..56026314, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (55784995..55788857, complement)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Activation of receptor-interacting protein 3-mediated necroptosis accelerates periodontitis in mice.
Title: Activation of receptor-interacting protein 3-mediated necroptosis accelerates periodontitis in mice.
Influenza virus infection activates TAK1 to suppress RIPK3-independent apoptosis and RIPK1-dependent necroptosis.
Title: Influenza virus infection activates TAK1 to suppress RIPK3-independent apoptosis and RIPK1-dependent necroptosis.
Expression of HIF1alpha in intestinal epithelium restricts arthritis inflammation by inhibiting RIPK3-induced cell death machinery.
Title: Expression of HIF1α in intestinal epithelium restricts arthritis inflammation by inhibiting RIPK3-induced cell death machinery.
Sensing of mitochondrial DNA by ZBP1 promotes RIPK3-mediated necroptosis and ferroptosis in response to diquat poisoning.
Title: Sensing of mitochondrial DNA by ZBP1 promotes RIPK3-mediated necroptosis and ferroptosis in response to diquat poisoning.
Derlin-1 promotes diet-induced non-alcoholic fatty liver disease via increasing RIPK3-mediated necroptosis.
Title: Derlin-1 promotes diet-induced non-alcoholic fatty liver disease via increasing RIPK3-mediated necroptosis.
Receptor interacting protein kinase-3 mediates both myopathy and cardiomyopathy in preclinical animal models of Duchenne muscular dystrophy.
Title: Receptor interacting protein kinase-3 mediates both myopathy and cardiomyopathy in preclinical animal models of Duchenne muscular dystrophy.
RIPK3 and kidney disease.
Title: RIPK3 and kidney disease.
RIPK3 promotes islet amyloid-induced beta-cell loss and glucose intolerance in a humanized mouse model of type 2 diabetes.
Title: RIPK3 promotes islet amyloid-induced β-cell loss and glucose intolerance in a humanized mouse model of type 2 diabetes.
Caspase cleavage of RIPK3 after Asp[333] is dispensable for mouse embryogenesis.
Title: Caspase cleavage of RIPK3 after Asp(333) is dispensable for mouse embryogenesis.
RIPK3 activation promotes DAXX-dependent neuronal necroptosis after intracerebral hemorrhage in mice.
Title: RIPK3 activation promotes DAXX-dependent neuronal necroptosis after intracerebral hemorrhage in mice.

Submit: New GeneRIF Correction See all GeneRIFs (245)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1)
Endonuclease-mediated (14) 1 citation
Targeted (15) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:237189

AW107945 (e-PCR)
- UniSTS:180664

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IEA Inferred from Electronic Annotation more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in T cell differentiation in thymus	IGI Inferred from Genetic Interaction more info	PubMed
involved_in T cell homeostasis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in activation of protein kinase activity	ISO Inferred from Sequence Orthology more info
involved_in activation of protein kinase activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in amyloid fibril formation	IEA Inferred from Electronic Annotation more info
involved_in amyloid fibril formation	ISO Inferred from Sequence Orthology more info
involved_in amyloid fibril formation	ISS Inferred from Sequence or Structural Similarity more info
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in cellular response to hydrogen peroxide	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to virus	IDA Inferred from Direct Assay more info	PubMed
involved_in defense response to virus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in execution phase of necroptosis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within execution phase of necroptosis	ISO Inferred from Sequence Orthology more info
involved_in lymph node development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in necroptotic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within necroptotic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in necroptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in necroptotic process	ISO Inferred from Sequence Orthology more info
involved_in necroptotic signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in necroptotic signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within_positive_effect non-canonical NF-kappaB signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of intrinsic apoptotic signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of ligase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of metabolic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of necroptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of necroptotic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of necroptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of necroptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of oxidoreductase activity	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of reactive oxygen species metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of reactive oxygen species metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of transferase activity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in programmed cell death	IEA Inferred from Electronic Annotation more info
involved_in programmed necrotic cell death	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of reactive oxygen species metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of T cell mediated cytotoxicity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of activated T cell proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of activation-induced cell death of T cells	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of adaptive immune response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of gene expression	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of reactive oxygen species metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of type II interferon production	IGI Inferred from Genetic Interaction more info	PubMed
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in spleen development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in thymus development	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in intracellular anatomical structure	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: receptor-interacting serine/threonine-protein kinase 3

Names: RIP-3; RIP-like protein kinase 3; mRIP3; receptor interacting protein 3

NP_001157579.1

EC 2.7.11.1

NP_001157580.1

EC 2.7.11.1

NP_064339.2

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001164107.1 → NP_001157579.1 receptor-interacting serine/threonine-protein kinase 3 isoform 2

See identical proteins and their annotated locations for NP_001157579.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the 5' region, compared to variant 1.This difference results in a distinct 5' UTR and causes translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 2) has a shorter N-terminus compared to isoform 1. Variant 2 and 3 encode the same isoform (2).

Source sequence(s)

AA270784, AC098877, AK012177, BF658559

Consensus CDS

CCDS49499.1

UniProtKB/TrEMBL

E9PZP3

Related

ENSMUSP00000137278.2, ENSMUST00000178399.3

Conserved Domains (2) summary

pfam12721
Location:346 → 393

RHIM; RIP homotypic interaction motif

cl21453
Location:1 → 220

PKc_like; Protein Kinases, catalytic domain
NM_001164108.1 → NP_001157580.1 receptor-interacting serine/threonine-protein kinase 3 isoform 2

See identical proteins and their annotated locations for NP_001157580.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate splice site in the 5' region, compared to variant 1.This difference results in a distinct 5' UTR and causes translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 2) has a shorter N-terminus compared to isoform 1. Variant 2 and 3 encode the same isoform (2).

Source sequence(s)

AA270784, AC098877, AK012177, BF658559, BY206463

Consensus CDS

CCDS49499.1

UniProtKB/TrEMBL

E9PZP3

Related

ENSMUSP00000126306.2, ENSMUST00000168716.8

Conserved Domains (2) summary

pfam12721
Location:346 → 393

RHIM; RIP homotypic interaction motif

cl21453
Location:1 → 220

PKc_like; Protein Kinases, catalytic domain
NM_019955.2 → NP_064339.2 receptor-interacting serine/threonine-protein kinase 3 isoform 1

See identical proteins and their annotated locations for NP_064339.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AA270784, AC098877, AK012177, BF658559, BY209344

Consensus CDS

CCDS27131.1

UniProtKB/Swiss-Prot

G3X8V8, Q3U3Z9, Q8K2Y2, Q9QZL0

Related

ENSMUSP00000022830.7, ENSMUST00000022830.14

Conserved Domains (3) summary

pfam00069
Location:24 → 284

Pkinase; Protein kinase domain

pfam12721
Location:410 → 457

RHIM; RIP homotypic interaction motif

cl21453
Location:28 → 284

PKc_like; Protein Kinases, catalytic domain