Tspan5 tetraspanin 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tspan5provided by MGI
Official Full Name: tetraspanin 5provided by MGI
Primary source: MGI:MGI:1928096
See related: Ensembl:ENSMUSG00000028152 AllianceGenome:MGI:1928096
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: NET-4; Tm4sf9; 2810455A09Rik; 4930505M03Rik
Summary: Enables enzyme binding activity. Involved in protein maturation. Predicted to be located in plasma membrane. Predicted to be integral component of plasma membrane. Is expressed in several structures, including brain; heart; inner ear; limb; and physiological umbilical hernia. Orthologous to human TSPAN5 (tetraspanin 5). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in cortex adult (RPKM 21.1), frontal lobe adult (RPKM 20.6) and 23 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3; 3 H1

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (138447609..138610194)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (138741848..138904433)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Tetraspanin-5-mediated MHC class I clustering is required for optimal CD8 T cell activation.
Title: Tetraspanin-5-mediated MHC class I clustering is required for optimal CD8 T cell activation.
two TspanC8-specific motifs in the large extracellular domain of Tspan5 that are important for ADAM10 interaction and exit from the endoplasmic reticulum. One of the anti-Tspan5 mAbs does not recognize Tspan5 associated with ADAM10, providing a convenient way to measure the fraction of Tspan5 not associated with ADAM10.
Title: New insights into the tetraspanin Tspan5 using novel monoclonal antibodies.
Tspan-5 and Tspan-10, are up-regulated during osteoclast differentiation and knockdown of their expression dramatically inhibits osteoclastogenesis
Title: Downregulation of Notch modulators, tetraspanin 5 and 10, inhibits osteoclastogenesis in vitro.
Up-regulated in pre-osteoclasts during cell differentiation.
Title: Expression and function of transmembrane-4 superfamily (tetraspanin) proteins in osteoclasts: reciprocal roles of Tspan-5 and NET-6 during osteoclastogenesis.
The level of Tspan-5 expression might be important for distinct phases of neuronal maturation.
Title: Tetraspanin-5 (Tm4sf9) mRNA expression parallels neuronal maturation in the cerebellum of normal and L7En-2 transgenic mice.
This protein is expressed during brain development from embryonic stages to adulthood.
Title: Pattern of expression of the tetraspanin Tspan-5 during brain development in the mouse.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Endonuclease-mediated (1)

General gene information

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Markers

08.MMHAP28FRC8.seq (e-PCR)
- UniSTS:122927

D3Mit194 (e-PCR), detects polymorphism
- UniSTS:129615

AU024142 (e-PCR)
- UniSTS:178942

Homology

Homologs of the Tspan5 gene: The Tspan5 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, zebrafish, C.elegans, and frog.
Orthologs from Annotation Pipeline: 342 organisms have orthologs with human gene Tspan5
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables enzyme binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in positive regulation of Notch signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in protein maturation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein maturation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: tetraspanin-5

Names: tetraspan NET-4; tetraspanin Tspan-5; transmembrane 4 superfamily member 9

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_019571.5 → NP_062517.1 tetraspanin-5

See identical proteins and their annotated locations for NP_062517.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein.

Source sequence(s)

AC110574, AC114621, AI447898, BC058695

Consensus CDS

CCDS17870.1

UniProtKB/Swiss-Prot

P62080

UniProtKB/TrEMBL

A0A6P5P905

Related

ENSMUSP00000029800.3, ENSMUST00000029800.9

Conserved Domains (2) summary

cd03159
Location:113 → 233

TM4SF9_like_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), TM4SF9_like subfamily. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment ...

pfam00335
Location:15 → 257

Tetraspannin; Tetraspanin family

RNA

NR_121195.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in an internal exon compared to variant 1. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC114621, AI447898, BC046784

Related

ENSMUST00000142890.8

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

138447609..138610194

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030252697.1 → XP_030108557.1 tetraspanin-5 isoform X2

UniProtKB/TrEMBL

A0A8C6GWG8, D3Z641

Related

ENSMUSP00000113230.2, ENSMUST00000119993.8

Conserved Domains (1) summary

cd03159
Location:42 → 162

TM4SF9_like_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), TM4SF9_like subfamily. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment ...
XM_006501737.5 → XP_006501800.1 tetraspanin-5 isoform X2

See identical proteins and their annotated locations for XP_006501800.1

UniProtKB/TrEMBL

A0A8C6GWG8, D3Z641

Conserved Domains (1) summary

cd03159
Location:42 → 162

TM4SF9_like_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), TM4SF9_like subfamily. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment ...
XM_006501736.5 → XP_006501799.1 tetraspanin-5 isoform X1

Conserved Domains (1) summary

cd03159
Location:156 → 276

TM4SF9_like_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), TM4SF9_like subfamily. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment ...