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PRKCQ protein kinase C theta [ Homo sapiens (human) ]

Gene ID: 5588, updated on 21-Dec-2019

Summary

Official Symbol
PRKCQprovided by HGNC
Official Full Name
protein kinase C thetaprovided by HGNC
Primary source
HGNC:HGNC:9410
See related
Ensembl:ENSG00000065675 MIM:600448
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
PRKCT; nPKC-theta
Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]
Expression
Broad expression in thyroid (RPKM 4.0), lymph node (RPKM 2.9) and 17 other tissues See more
Orthologs

Genomic context

See PRKCQ in Genome Data Viewer
Location:
10p15.1
Exon count:
23
Annotation release Status Assembly Chr Location
109.20191205 current GRCh38.p13 (GCF_000001405.39) 10 NC_000010.11 (6393038..6580646, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 10 NC_000010.10 (6435558..6622254, complement)

Chromosome 10 - NC_000010.11Genomic Context describing neighboring genes Neighboring gene long intergenic non-protein coding RNA 2649 Neighboring gene developmental pluripotency associated 5 pseudogene 3 Neighboring gene long intergenic non-protein coding RNA 2656 Neighboring gene uncharacterized LOC107984202 Neighboring gene Sharpr-MPRA regulatory region 12587 Neighboring gene PRKCQ antisense RNA 1 Neighboring gene long intergenic non-protein coding RNA 2648

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phenotypes

NHGRI GWAS Catalog

Description
A genome-wide association study of bronchodilator response in asthmatics.
NHGRI GWA Catalog
Common variants at CD40 and other loci confer risk of rheumatoid arthritis.
NHGRI GWA Catalog
Genetics of rheumatoid arthritis contributes to biology and drug discovery.
NHGRI GWA Catalog
Genome-wide association study and meta-analysis find that over 40 loci affect risk of type 1 diabetes.
NHGRI GWA Catalog
Genome-wide association study meta-analysis identifies seven new rheumatoid arthritis risk loci.
NHGRI GWA Catalog
Genome-wide association study of antibody response to smallpox vaccine.
NHGRI GWA Catalog
Genome-wide association study of body mass index in 23 000 individuals with and without asthma.
NHGRI GWA Catalog
Genome-wide association study of celiac disease in North America confirms FRMD4B as new celiac locus.
NHGRI GWA Catalog
Meta-analysis of genome-wide association study data identifies additional type 1 diabetes risk loci.
NHGRI GWA Catalog

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120 activates forward trafficking and surface clustering of NMDA receptors in membrane micro domains by a PKA-dependent phosphorylation of the NR1 C-terminal Ser897, followed by a PKC-dependent phosphorylation of Ser896 PubMed
env Down modulation of the interaction between HIV-1 gp120 and CD4 by TPA is blocked by protein kinase C (PKC) inhibitors, suggesting PKC may play an important role in HIV-1 infection PubMed
env c-FLIPL inhibits Bax activation via modulating PKC expression at the transcriptional level involving AP-2 during gp120 treatment PubMed
env Pre-treatment of endothelial cells with fibroblast growth factor 2 (FGF2) protects cells from HIV-1 gp120 angiotoxicity; this protection is regulated by crosstalk among the ERK, PI3K-AKT and PKC signaling pathways PubMed
env Induction of apoptosis in cell cultures through binding of HIV-1 gp120 or gp160 to CXCR4 involves protein kinase C, basic fibroblast growth factor, caspase-3, and the pro-apoptotic molecule Bax PubMed
env HIV-1 gp120 increases the levels of calcium-dependent and -independent PKC isozymes; the most striking change is observed in PKC-zeta isozyme levels PubMed
env A specific interaction between CD4 and HIV-1 gp120 is required for phosphorylation of CD4, which could involve protein kinase C PubMed
env IL-16 induces rapid translocation of PKC from the cytosol to the membrane in CD4+ cells; PKC inhibitors completely block IL-16-induced lymphocyte migration as well as the motile response induced by HIV-1 gp120 and anti-CD4 antibody binding to CD4 PubMed
Envelope surface glycoprotein gp160, precursor env HIV-1 gp160-induced AP-1 complex formation is dependent upon protein tyrosine phosphorylation and is abolished by inhibitors of protein kinase C, but it is unaffected by calcium channel blockers or cyclosporine A PubMed
Envelope transmembrane glycoprotein gp41 env A synthetic peptide corresponding to cytoplasmic domain residues 828-848 of HIV-1 gp41 inhibits pKC-catalysed phosphorylation of a protein substrate PubMed
env A synthetic peptide containing residues 581-597 from HIV-1 gp41 inhibits protein kinase C (pkC)-mediated phosphorylation of the CD3 gamma-chain in intact cells and directly inhibits partially purified pkC PubMed
Nef nef PKCtheta is a downstream effector of LCK and is recruited by HIV-1 Nef to form the Nef/LCK/PKCtheta complex, which promotes HIV transcription PubMed
nef The formation of Nef/LCK/PKCtheta complex activates the ERK MAPK signaling pathway PubMed
nef Interactions between HIV-1 Nef and PKC isozymes in Jurkat cells show that Nef interacts preferentially with PKCtheta; the interaction of Nef and PKCtheta is independent of calcium, but enhanced by phospholipid activators of PKC PubMed
Tat tat HIV-1 Tat relocalizes PRKCQ (PKC-theta) and RELA (NFkB) to the nucleus of Jurkat T cells that stably express Tat PubMed
tat HIV-1 Tat induces phosphorylation of Threonine538 in PRKCQ (PKC-theta) in Jurkat T cells stably expressing Tat PubMed
tat HIV-1 Tat activates PRKCQ (PKC-theta) kinase activity, which leads to RELA (NFkB), NRAS, RAF1, MAP2K1 (MEK1), MAP2K2 (MEK2), MAPK3 (ERK1), and MAPK1 (ERK2) activation, in Jurkat T cells that stably express Tat PubMed
tat HIV-1 Tat upregulates PRKCQ (PKC-theta) expression in Jurkat T cells stably expressing Tat PubMed
tat HIV-1 Tat activates protein kinase C, resulting in the induction of TNF-alpha, IL-6 and IL-10 expression and the secretion of MCP-1 PubMed
tat Tat-triggered PKCdelta and PKCtheta activation results in the downstream signaling through the apoptosis pathways mediated by both ERK1/2 and caspase-3 PubMed
tat Phospholipase C/protein kinase C signaling pathway-dependent phosphorylation of p44/42 and JNK MAP kinases participates partially in IL-1beta induction by TAT PubMed
tat Protein kinase C is required for HIV-1 Tat-mediated transactivation of the viral LTR promoter, indicating protein kinase C regulates the process of HIV-1 transactivation and may play a role in the transition of HIV from latency to productive growth PubMed
tat Protein kinase C phosphorylates HIV-1 Tat on serine residue 46 PubMed
matrix gag Protein kinase C (PKC) phosphorylates HIV-1 Matrix on serine residue 111 resulting in a shift in localization of Matrix from the cytosol to the cellular membrane, suggesting a myristoyl-protein switch regulated by PKC phosphorylation PubMed
retropepsin gag-pol Phosphorylation of human recombinant vimentin by PKC inhibits the proteolytic processing of the vimentin head domain by HIV-1 protease PubMed
reverse transcriptase gag-pol HIV-1 RT heterodimer expressed in bacteria can be phosphorylated in vitro by several purified mammalian protein kinases including auto-activated protein kinase (PK), CKII, cytosolic protamine kinase (CPK), myelin basic protein kinase 1 (MBPK1), and PRKC PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • MGC126514, MGC141919

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
metal ion binding IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein kinase C activity IEA
Inferred from Electronic Annotation
more info
 
protein kinase activity TAS
Traceable Author Statement
more info
 
protein serine/threonine kinase activity EXP
Inferred from Experiment
more info
PubMed 
protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
protein serine/threonine kinase activity NAS
Non-traceable Author Statement
more info
PubMed 
Process Evidence Code Pubs
Fc-epsilon receptor signaling pathway TAS
Traceable Author Statement
more info
 
T cell receptor signaling pathway TAS
Traceable Author Statement
more info
 
axon guidance TAS
Traceable Author Statement
more info
 
cell chemotaxis IMP
Inferred from Mutant Phenotype
more info
PubMed 
inflammatory response IEA
Inferred from Electronic Annotation
more info
 
intracellular signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
intracellular signal transduction NAS
Non-traceable Author Statement
more info
PubMed 
membrane protein ectodomain proteolysis ISS
Inferred from Sequence or Structural Similarity
more info
 
negative regulation of T cell apoptotic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
negative regulation of insulin receptor signaling pathway IMP
Inferred from Mutant Phenotype
more info
PubMed 
peptidyl-serine phosphorylation IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
platelet activation TAS
Traceable Author Statement
more info
 
positive regulation of NF-kappaB transcription factor activity IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of T cell activation ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of T cell proliferation IEA
Inferred from Electronic Annotation
more info
 
positive regulation of T-helper 17 type immune response ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of T-helper 2 cell activation ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of interleukin-17 production ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of interleukin-2 biosynthetic process IEA
Inferred from Electronic Annotation
more info
 
positive regulation of interleukin-4 production ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of telomerase activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of telomere capping IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of telomere maintenance via telomerase IMP
Inferred from Mutant Phenotype
more info
PubMed 
regulation of cell growth NAS
Non-traceable Author Statement
more info
PubMed 
regulation of megakaryocyte differentiation TAS
Traceable Author Statement
more info
 
regulation of platelet aggregation ISS
Inferred from Sequence or Structural Similarity
more info
 
regulation of transcription, DNA-templated ISS
Inferred from Sequence or Structural Similarity
more info
 
Component Evidence Code Pubs
aggresome IDA
Inferred from Direct Assay
more info
 
centriolar satellite IDA
Inferred from Direct Assay
more info
 
cytosol TAS
Traceable Author Statement
more info
 
immunological synapse IEA
Inferred from Electronic Annotation
more info
 
plasma membrane TAS
Traceable Author Statement
more info
 

General protein information

Preferred Names
protein kinase C theta type
NP_001229342.1
NP_001269573.1
NP_001269574.1
NP_001310194.1
NP_001310195.1
NP_001310196.1
NP_006248.1
XP_005252553.1
XP_005252554.1
XP_024303844.1
XP_024303845.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001242413.2NP_001229342.1  protein kinase C theta type isoform 2

    See identical proteins and their annotated locations for NP_001229342.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.
    Source sequence(s)
    AA234440, AK293935, AK308099, AK312411, AY702977, BX647657, L01087, R40214
    Consensus CDS
    CCDS55701.1
    UniProtKB/Swiss-Prot
    Q04759
    Related
    ENSP00000380361.2, ENST00000397176.6
    Conserved Domains (3) summary
    PTZ00263
    Location:376631
    PTZ00263; protein kinase A catalytic subunit; Provisional
    pfam00130
    Location:232284
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
    cl21453
    Location:374641
    PKc_like; Protein Kinases, catalytic domain
  2. NM_001282644.2NP_001269573.1  protein kinase C theta type isoform 3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) uses an alternate splice site in the 5' region. This difference results in translation initiation at an alternate AUG and results in an isoform (3) with a shorter and distinct N-terminus, compared to isoform 1.
    Source sequence(s)
    AA234440, AK308099, BX647657, R40214
    UniProtKB/Swiss-Prot
    Q04759
    Conserved Domains (3) summary
    smart00220
    Location:344598
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd05619
    Location:338668
    STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta
    pfam00130
    Location:196248
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  3. NM_001282645.1NP_001269574.1  protein kinase C theta type isoform 4

    See identical proteins and their annotated locations for NP_001269574.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) lacks an exon in the 5' region. This difference results in translation initiation at an alternate AUG and results in an isoform (4) with a shorter and distinct N-terminus, compared to isoform 1.
    Source sequence(s)
    AA234440, AK293935, AK308099, BX647657, L01087, R40214
    Consensus CDS
    CCDS60482.1
    UniProtKB/Swiss-Prot
    Q04759
    Related
    ENSP00000441752.1, ENST00000539722.5
    Conserved Domains (2) summary
    cd05619
    Location:249579
    STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta
    pfam00130
    Location:107159
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  4. NM_001323265.1NP_001310194.1  protein kinase C theta type isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (5) and variant 1 both encode isoform 1.
    Source sequence(s)
    AL137145, AL158043
    Consensus CDS
    CCDS7079.1
    UniProtKB/Swiss-Prot
    Q04759
    Conserved Domains (2) summary
    cd05619
    Location:374704
    STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta
    pfam00130
    Location:232284
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  5. NM_001323266.2NP_001310195.1  protein kinase C theta type isoform 4

    Status: VALIDATED

    Description
    Transcript Variant: This variant (6) and variant 4 both encode isoform 4.
    Source sequence(s)
    AL137145, AL158043
    Consensus CDS
    CCDS60482.1
    UniProtKB/Swiss-Prot
    Q04759
    Conserved Domains (2) summary
    cd05619
    Location:249579
    STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta
    pfam00130
    Location:107159
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  6. NM_001323267.1NP_001310196.1  protein kinase C theta type isoform 5

    Status: VALIDATED

    Source sequence(s)
    AL137145, AL158043
  7. NM_006257.5NP_006248.1  protein kinase C theta type isoform 1

    See identical proteins and their annotated locations for NP_006248.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 5 both encode the same isoform (1).
    Source sequence(s)
    AA234440, AK293935, AK308099, AK312411, BX647657, R40214
    Consensus CDS
    CCDS7079.1
    UniProtKB/Swiss-Prot
    Q04759
    Related
    ENSP00000263125.5, ENST00000263125.10
    Conserved Domains (2) summary
    cd05619
    Location:374704
    STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta
    pfam00130
    Location:232284
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.20191205

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000010.11 Reference GRCh38.p13 Primary Assembly

    Range
    6393038..6580646 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_005252497.4XP_005252554.1  protein kinase C theta type isoform X3

    Conserved Domains (3) summary
    smart00220
    Location:414668
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    pfam00130
    Location:266318
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
    cl21453
    Location:408695
    PKc_like; Protein Kinases, catalytic domain
  2. XM_024448077.1XP_024303845.1  protein kinase C theta type isoform X4

    Conserved Domains (2) summary
    cd05619
    Location:249579
    STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta
    pfam00130
    Location:107159
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  3. XM_024448076.1XP_024303844.1  protein kinase C theta type isoform X2

    Conserved Domains (2) summary
    cd05619
    Location:374704
    STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta
    pfam00130
    Location:232284
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  4. XM_005252496.4XP_005252553.1  protein kinase C theta type isoform X1

    Conserved Domains (3) summary
    smart00220
    Location:414668
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd05619
    Location:408738
    STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta
    pfam00130
    Location:266318
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
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