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PRKCA protein kinase C alpha [ Homo sapiens (human) ]

Gene ID: 5578, updated on 24-Nov-2020

Summary

Official Symbol
PRKCAprovided by HGNC
Official Full Name
protein kinase C alphaprovided by HGNC
Primary source
HGNC:HGNC:9393
See related
Ensembl:ENSG00000154229 MIM:176960
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha
Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in brain (RPKM 12.7), adrenal (RPKM 6.5) and 22 other tissues See more
Orthologs

Genomic context

See PRKCA in Genome Data Viewer
Location:
17q24.2
Exon count:
24
Annotation release Status Assembly Chr Location
109.20201120 current GRCh38.p13 (GCF_000001405.39) 17 NC_000017.11 (66302613..66810743)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (64298760..64806862)

Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene RNA, 5S ribosomal pseudogene 444 Neighboring gene CRISPRi-validated cis-regulatory element chr17.4440 Neighboring gene Sharpr-MPRA regulatory region 10613 Neighboring gene RNA, 7SL, cytoplasmic 735, pseudogene Neighboring gene Sharpr-MPRA regulatory region 12714 Neighboring gene PRKCA antisense RNA 1 Neighboring gene RNA, U6 small nuclear 928, pseudogene Neighboring gene RNA, 5S ribosomal pseudogene 445 Neighboring gene microRNA 634 Neighboring gene calcium voltage-gated channel auxiliary subunit gamma 5 Neighboring gene RNA, 5S ribosomal pseudogene 446

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phenotypes

Associated conditions

Description Tests
A genome-wide search for quantitative trait loci affecting the cortical surface area and thickness of Heschl's gyrus.
GeneReviews: Not available
Genetic association study of QT interval highlights role for calcium signaling pathways in myocardial repolarization.
GeneReviews: Not available
Genetics of coronary artery calcification among African Americans, a meta-analysis.
GeneReviews: Not available
Genome-wide association study identifies new susceptibility loci for posttraumatic stress disorder.
GeneReviews: Not available
Genome-wide association study of body height in African Americans: the Women's Health Initiative SNP Health Association Resource (SHARe).
GeneReviews: Not available

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120-treated vaginal epithelial cells show downregulation of protein kinase C, alpha (PRKCA) expression as compared to untreated control PubMed
env HIV-1 gp120 activates forward trafficking and surface clustering of NMDA receptors in membrane microdomains by a PKA-dependent phosphorylation of the NR1 C-terminal Ser897, followed by a PKC-dependent phosphorylation of Ser896 PubMed
env Down modulation of the interaction between HIV-1 gp120 and CD4 by TPA is blocked by protein kinase C (PKC) inhibitors, suggesting PKC may play an important role in HIV-1 infection PubMed
env PKC-dependent pathway, particularly PKCalpha and PKCbeta1, requires HIV-1 gp120-mediated Rac-1 activation and membrane fusion PubMed
env c-FLIPL inhibits Bax activation via modulating PKC expression at the transcriptional level involving AP-2 during gp120 treatment PubMed
env Pre-treatment of endothelial cells with fibroblast growth factor 2 (FGF2) protects cells from HIV-1 gp120 angiotoxicity; this protection is regulated by crosstalk among the ERK, PI3K-AKT and PKC signaling pathways PubMed
env Induction of apoptosis in cell cultures through binding of HIV-1 gp120 or gp160 to CXCR4 involves protein kinase C, basic fibroblast growth factor, caspase-3, and the pro-apoptotic molecule Bax PubMed
env HIV-1 gp120 increases the levels of calcium-dependent and -independent PKC isozymes; the most striking change is observed in PKC-zeta isozyme levels PubMed
env A specific interaction between CD4 and HIV-1 gp120 is required for phosphorylation of CD4, which could involve protein kinase C PubMed
env IL-16 induces rapid translocation of PKC from the cytosol to the membrane in CD4+ cells; PKC inhibitors completely block IL-16-induced lymphocyte migration as well as the motile response induced by HIV-1 gp120 and anti-CD4 antibody binding to CD4 PubMed
Envelope surface glycoprotein gp160, precursor env HIV-1 gp160-induced Ca(2+) influx reduction is antagonized by an inhibitor acting especially on PKC alpha and PKC beta I; Western blotting analyses show that the cellular distribution of PKC alpha and -beta I are significantly modified by gp160 PubMed
env HIV-1 gp160-induced AP-1 complex formation is dependent upon protein tyrosine phosphorylation and is abolished by inhibitors of protein kinase C, but it is unaffected by calcium channel blockers or cyclosporine A PubMed
Envelope transmembrane glycoprotein gp41 env A synthetic peptide corresponding to cytoplasmic domain residues 828-848 of HIV-1 gp41 inhibits pKC-catalysed phosphorylation of a protein substrate PubMed
env A synthetic peptide containing residues 581-597 from HIV-1 gp41 inhibits protein kinase C (pkC)-mediated phosphorylation of the CD3 gamma-chain in intact cells and directly inhibits partially purified pkC PubMed
Nef nef PKC activity and serine 6 in HIV-1 Nef are required for Nef-supported transcription, infectivity, and replication PubMed
nef Treatment of cells with protein kinase C (PKC) inhibitors reduces HIV-1 Nef phosphorylation in vivo, and phosphorylation of Nef increases 8- to 12-fold after treatment with phorbol myristate acetate; these data indicate PKC phosphorylates Nef PubMed
nef Protein kinase C phosphorylates HIV-1 Nef at threonine 15 PubMed
Rev rev PKC has been shown to phosphorylate Rev in vitro, an effect associated with accelerated formation of an efficient RNA-binding conformation for Rev PubMed
Tat tat The gene expression of protein kinase C alpha subunit is significantly upregulated in both clade B and clade C Tat treated SK-N-MC neuroblastoma cells PubMed
tat HIV-1 Tat downregulates the expression of protein kinase C, alpha (PRKCA) in human primary T cells PubMed
tat Phospholipase C/protein kinase C signaling pathway-dependent phosphorylation of p44/42 and JNK MAP kinases participates partially in IL-1beta induction by TAT PubMed
tat HIV-1 Tat-peptide(48-60) inhibits protein kinase C alpha and protein kinase A in a concentration-dependent manner PubMed
tat Protein kinase C phosphorylates HIV-1 Tat on serine residue 46 PubMed
tat Protein kinase C is required for HIV-1 Tat-mediated transactivation of the viral LTR promoter, indicating protein kinase C regulates the process of HIV-1 transactivation and may play a role in the transition of HIV from latency to productive growth PubMed
tat HIV-1 Tat activates protein kinase C, resulting in the induction of TNF-alpha, IL-6 and IL-10 expression and the secretion of MCP-1 PubMed
matrix gag Protein kinase C (PKC) phosphorylates HIV-1 Gag and Matrix on serine residue 111 resulting in a shift in localization of Matrix from the cytosol to the cellular membrane, suggesting a myristoyl-protein switch regulated by PKC phosphorylation PubMed
retropepsin gag-pol Phosphorylation of human recombinant vimentin by PKC inhibits the proteolytic processing of the vimentin head domain by HIV-1 protease PubMed
reverse transcriptase gag-pol HIV-1 RT heterodimer expressed in bacteria can be phosphorylated in vitro by several purified mammalian protein kinases including auto-activated protein kinase (PK), CKII, cytosolic protamine kinase (CPK), myelin basic protein kinase 1 (MBPK1), and PRKC PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • MGC129900, MGC129901

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
calcium-dependent protein kinase C activity IEA
Inferred from Electronic Annotation
more info
 
enzyme binding IDA
Inferred from Direct Assay
more info
PubMed 
histone kinase activity (H3-T6 specific) IDA
Inferred from Direct Assay
more info
PubMed 
integrin binding ISS
Inferred from Sequence or Structural Similarity
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein kinase C activity ISS
Inferred from Sequence or Structural Similarity
more info
 
protein kinase activity IDA
Inferred from Direct Assay
more info
PubMed 
protein serine/threonine kinase activity EXP
Inferred from Experiment
more info
PubMed 
protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
protein serine/threonine kinase activity TAS
Traceable Author Statement
more info
 
zinc ion binding IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
ERBB2 signaling pathway TAS
Traceable Author Statement
more info
 
angiogenesis IEA
Inferred from Electronic Annotation
more info
 
apoptotic signaling pathway TAS
Traceable Author Statement
more info
PubMed 
axon guidance TAS
Traceable Author Statement
more info
 
cell adhesion IEA
Inferred from Electronic Annotation
more info
 
desmosome assembly IMP
Inferred from Mutant Phenotype
more info
PubMed 
histone H3-T6 phosphorylation IDA
Inferred from Direct Assay
more info
PubMed 
intracellular signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
mitotic nuclear envelope disassembly TAS
Traceable Author Statement
more info
 
negative regulation of glial cell apoptotic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
peptidyl-serine phosphorylation IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
peptidyl-serine phosphorylation TAS
Traceable Author Statement
more info
PubMed 
peptidyl-threonine phosphorylation ISS
Inferred from Sequence or Structural Similarity
more info
PubMed 
platelet activation TAS
Traceable Author Statement
more info
 
positive regulation of ERK1 and ERK2 cascade ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of angiogenesis IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of blood vessel endothelial cell migration IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of bone resorption ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of cardiac muscle hypertrophy ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of cell adhesion IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of cell migration IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of dense core granule biogenesis ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of endothelial cell migration IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of endothelial cell proliferation IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of lipopolysaccharide-mediated signaling pathway IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of macrophage differentiation ISS
Inferred from Sequence or Structural Similarity
more info
 
positive regulation of mitotic cell cycle IMP
Inferred from Mutant Phenotype
more info
PubMed 
protein phosphorylation IDA
Inferred from Direct Assay
more info
PubMed 
regulation of insulin secretion TAS
Traceable Author Statement
more info
 
regulation of mRNA stability TAS
Traceable Author Statement
more info
 
regulation of platelet aggregation IDA
Inferred from Direct Assay
more info
PubMed 
response to interleukin-1 IMP
Inferred from Mutant Phenotype
more info
PubMed 
Component Evidence Code Pubs
alphav-beta3 integrin-PKCalpha complex ISS
Inferred from Sequence or Structural Similarity
more info
 
cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
cytosol IDA
Inferred from Direct Assay
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
endoplasmic reticulum IDA
Inferred from Direct Assay
more info
PubMed 
extracellular exosome HDA PubMed 
mitochondrial membrane IEA
Inferred from Electronic Annotation
more info
 
mitochondrion HDA PubMed 
nucleoplasm TAS
Traceable Author Statement
more info
 
perinuclear region of cytoplasm ISS
Inferred from Sequence or Structural Similarity
more info
 
plasma membrane IDA
Inferred from Direct Assay
more info
PubMed 
plasma membrane TAS
Traceable Author Statement
more info
 

General protein information

Preferred Names
protein kinase C alpha type
Names
PKC-A
aging-associated gene 6
NP_002728.2
XP_016880325.1
XP_016880326.1
XP_016880330.1
XP_024306597.1
XP_024306598.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_012206.1 RefSeqGene

    Range
    5001..512937
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_002737.3NP_002728.2  protein kinase C alpha type

    Status: REVIEWED

    Source sequence(s)
    AC005918, AC005988, AC006263, AC006947, AC009452
    Consensus CDS
    CCDS11664.1
    Related
    ENSP00000408695.3, ENST00000413366.8
    Conserved Domains (3) summary
    cd04026
    Location:159289
    C2_PKC_alpha_gamma; C2 domain in Protein Kinase C (PKC) alpha and gamma
    cd05615
    Location:328668
    STKc_cPKC_alpha; Catalytic domain of the Serine/Threonine Kinase, Classical Protein Kinase C alpha
    pfam00130
    Location:3789
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.20201120

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000017.11 Reference GRCh38.p13 Primary Assembly

    Range
    66302613..66810743
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017024836.2XP_016880325.1  protein kinase C alpha type isoform X1

  2. XM_017024841.1XP_016880330.1  protein kinase C alpha type isoform X4

  3. XM_017024837.1XP_016880326.1  protein kinase C alpha type isoform X2

  4. XM_024450830.1XP_024306598.1  protein kinase C alpha type isoform X3

    Conserved Domains (3) summary
    cd04026
    Location:73203
    C2_PKC_alpha_gamma; C2 domain in Protein Kinase C (PKC) alpha and gamma
    cd05615
    Location:242582
    STKc_cPKC_alpha; Catalytic domain of the Serine/Threonine Kinase, Classical Protein Kinase C alpha
    pfam00130
    Location:1668
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  5. XM_024450829.1XP_024306597.1  protein kinase C alpha type isoform X3

    Conserved Domains (3) summary
    cd04026
    Location:73203
    C2_PKC_alpha_gamma; C2 domain in Protein Kinase C (PKC) alpha and gamma
    cd05615
    Location:242582
    STKc_cPKC_alpha; Catalytic domain of the Serine/Threonine Kinase, Classical Protein Kinase C alpha
    pfam00130
    Location:1668
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

RNA

  1. XR_001752558.1 RNA Sequence

    Related
    ENST00000578063.5
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