Format

Send to:

Choose Destination

PPP1R8 protein phosphatase 1 regulatory subunit 8 [ Homo sapiens (human) ]

Gene ID: 5511, updated on 6-Jan-2019

Summary

Official Symbol
PPP1R8provided by HGNC
Official Full Name
protein phosphatase 1 regulatory subunit 8provided by HGNC
Primary source
HGNC:HGNC:9296
See related
Ensembl:ENSG00000117751 MIM:602636
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
Summary
This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in endometrium (RPKM 13.4), lymph node (RPKM 13.2) and 25 other tissues See more
Orthologs

Genomic context

See PPP1R8 in Genome Data Viewer
Location:
1p35.3
Exon count:
7
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 1 NC_000001.11 (27830741..27851672)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (28157252..28178183)

Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene syntaxin 12 Neighboring gene RNA, U6 small nuclear 1245, pseudogene Neighboring gene small Cajal body-specific RNA 1 Neighboring gene thymocyte selection associated family member 2 Neighboring gene replication protein A2

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of protein phosphatase 1, regulatory subunit 8 (PPP1R8) by shRNA library screening inhibits HIV-1 replication in cultured Jurkat T-cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Tat tat PP1 interacts with Tat in part through the binding of Val(36) and Phe(38) of Tat to PP1, and Tat is involved in the nuclear and subnuclear targeting of PP1 PubMed
tat Nuclear inhibitor of PP1 (NIPP1) inhibits HIV-1 Tat-induced transcription, an effect that can be reversed by co-expression of PP1, indicating Tat function is regulated by PP1/NIPP1 PubMed

Go to the HIV-1, Human Interaction Database

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
DNA binding IEA
Inferred from Electronic Annotation
more info
 
RNA binding TAS
Traceable Author Statement
more info
PubMed 
endonuclease activity IEA
Inferred from Electronic Annotation
more info
 
mRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein serine/threonine phosphatase inhibitor activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
ribonuclease E activity TAS
Traceable Author Statement
more info
PubMed 
Process Evidence Code Pubs
RNA catabolic process TAS
Traceable Author Statement
more info
PubMed 
RNA phosphodiester bond hydrolysis IEA
Inferred from Electronic Annotation
more info
 
RNA splicing IEA
Inferred from Electronic Annotation
more info
 
cell proliferation IEA
Inferred from Electronic Annotation
more info
 
mRNA processing IEA
Inferred from Electronic Annotation
more info
 
negative regulation of phosphoprotein phosphatase activity IEA
Inferred from Electronic Annotation
more info
 
negative regulation of protein dephosphorylation IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
production of miRNAs involved in gene silencing by miRNA IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Component Evidence Code Pubs
cytoplasm IEA
Inferred from Electronic Annotation
more info
 
nuclear speck IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
nuclear speck IDA
Inferred from Direct Assay
more info
PubMed 
nucleoplasm IDA
Inferred from Direct Assay
more info
 
nucleus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
spliceosomal complex IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
nuclear inhibitor of protein phosphatase 1
Names
RNase E
activator of RNA decay
nuclear inhibitor of protein phosphatase-1 alpha
nuclear inhibitor of protein phosphatase-1 beta
nuclear subunit of PP-1
protein phosphatase 1, regulatory (inhibitor) subunit 8

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_002713.3NP_002704.1  nuclear inhibitor of protein phosphatase 1 isoform gamma

    See identical proteins and their annotated locations for NP_002704.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) encodes the shortest isoform (gamma, also called ARD-1). It has its first exon longer at the 3' end and lacks an internal exon as compared to that of transcript variant 1. The gamma isoform uses a different translation start site and is N-terminally truncated as compared to isoform alpha encoded by variant 1. Isoform gamma contains ribonuclease activity.
    Source sequence(s)
    AF061959, DA077576, U14575
    Consensus CDS
    CCDS313.1
    UniProtKB/Swiss-Prot
    Q12972
    UniProtKB/TrEMBL
    Q6ICT4
    Related
    ENSP00000236412.7, ENST00000236412.11
  2. NM_014110.5NP_054829.2  nuclear inhibitor of protein phosphatase 1 isoform alpha

    See identical proteins and their annotated locations for NP_054829.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) is the shortest transcript but encodes the predominant and longest isoform (alpha). Isoform alpha contains protein phosphatase inhibitory actvity.
    Source sequence(s)
    AF061959, AK292077, AL109927, DA167043
    Consensus CDS
    CCDS311.1
    UniProtKB/Swiss-Prot
    Q12972
    Related
    ENSP00000311677.5, ENST00000311772.9
    Conserved Domains (2) summary
    cd00060
    Location:27119
    FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
    pfam11945
    Location:98212
    WASH_WAHD; WAHD domain of WASH complex
  3. NM_138558.2NP_612568.1  nuclear inhibitor of protein phosphatase 1 isoform beta/delta

    See identical proteins and their annotated locations for NP_612568.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) is the longest transcript and has its first exon longer at the 3' end as compared to that of transcript variant 1. The resulting isoform (beta/delta) uses a different translation start site and is N-terminally truncated as compared to isoform alpha encoded by variant 1. Isoform beta/delta contains protein phosphatase inhibitory activity.
    Source sequence(s)
    AF061959, DA693246
    Consensus CDS
    CCDS312.1
    UniProtKB/Swiss-Prot
    Q12972
    Related
    ENSP00000363042.4, ENST00000373931.8

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000001.11 Reference GRCh38.p12 Primary Assembly

    Range
    27830741..27851672
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017001594.2XP_016857083.1  nuclear inhibitor of protein phosphatase 1 isoform X1

    UniProtKB/Swiss-Prot
    Q12972
    UniProtKB/TrEMBL
    Q6ICT4
Support Center