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MPHOSPH8 M-phase phosphoprotein 8 [ Homo sapiens (human) ]

Gene ID: 54737, updated on 23-Nov-2023

Summary

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Official Symbol
MPHOSPH8provided by HGNC
Official Full Name
M-phase phosphoprotein 8provided by HGNC
Primary source
HGNC:HGNC:29810
See related
Ensembl:ENSG00000196199 MIM:611626; AllianceGenome:HGNC:29810
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
TWA3; mpp8; HSMPP8
Summary
Enables chromatin binding activity and methylated histone binding activity. Involved in negative regulation of single stranded viral RNA replication via double stranded DNA intermediate; regulation of DNA methylation; and regulation of gene expression. Located in several cellular components, including cytosol; heterochromatin; and nuclear lumen. Part of nucleosome. [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in ovary (RPKM 20.0), spleen (RPKM 15.8) and 25 other tissues See more
Orthologs
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Genomic context

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See MPHOSPH8 in Genome Data Viewer
Location:
13q12.11
Exon count:
16
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 13 NC_000013.11 (19633659..19673441)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 13 NC_060937.1 (18797911..18869507)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 13 NC_000013.10 (20207799..20247581)

Chromosome 13 - NC_000013.11Genomic Context describing neighboring genes Neighboring gene transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 Neighboring gene uncharacterized LOC124903271 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5137 Neighboring gene mitochondrial ribosomal protein S31 pseudogene 2 Neighboring gene TPTE2 antisense RNA 1 Neighboring gene GOLM2 pseudogene 1 Neighboring gene estrogen-related receptor alpha pseudogene 1 Neighboring gene ReSE screen-validated silencer GRCh37_chr13:20161162-20161342 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7403 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7404 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7405 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr13:20173929-20174918 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5138 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5139 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7406 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7407 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7408 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr13:20219053-20219554 Neighboring gene paraspeckle component 1 Neighboring gene H3K27ac hESC enhancer GRCh37_chr13:20316454-20317223 Neighboring gene RNA, 7SL, cytoplasmic 166, pseudogene Neighboring gene H3K27ac hESC enhancer GRCh37_chr13:20340248-20340748 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr13:20343088-20343647 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7409 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr13:20392071-20392598 Neighboring gene ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 4 pseudogene 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. MPP8 Promotes Proliferation and Restrains Apoptosis in Osteosarcoma by Regulating p38αMAPK Pathway. Li T, et al. Technol Cancer Res Treat, 2021 Jan-Dec. PMID 33596786, Free PMC Article
  2. M-phase phosphoprotein 8 promotes gastric cancer growth and metastasis via p53/Bcl-2 and EMT-related signaling pathways. Wang Y, et al. J Cell Biochem, 2020 Mar. PMID 31692032
  3. Humanin binds MPP8: mapping interaction sites of the peptide and protein. Maximov VV, et al. J Pept Sci, 2013 May. PMID 23532874
  4. Mitotic phosphorylation of MPP8 by cyclin-dependent kinases regulates chromatin dissociation. Nishigaki M, et al. Biochem Biophys Res Commun, 2013 Mar 22. PMID 23416073
  5. Structural basis for specific binding of human MPP8 chromodomain to histone H3 methylated at lysine 9. Li J, et al. PLoS One, 2011. PMID 22022377, Free PMC Article

See all (52) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. MPP8 Promotes Proliferation and Restrains Apoptosis in Osteosarcoma by Regulating p38alphaMAPK Pathway.
    Title: MPP8 Promotes Proliferation and Restrains Apoptosis in Osteosarcoma by Regulating p38αMAPK Pathway.
  2. M-phase phosphoprotein 8 promotes gastric cancer growth and metastasis via p53/Bcl-2 and EMT-related signaling pathways.
    Title: M-phase phosphoprotein 8 promotes gastric cancer growth and metastasis via p53/Bcl-2 and EMT-related signaling pathways.
  3. The study demonstrated that MPP8 was associated with non-small cell lung cancer cell proliferation through regulation of HOXA5.
    Title: Knockdown of MPP8 suppresses cell proliferation via regulation of HOXA5 in non-small cell lung cancer cells.
  4. The haploid screen identified HUSH, an epigenetic heterochromatin repressor complex composed of three subunits, TASOR, MPP8 and Periphilin. (Review)
    Title: Position-effect variegation revisited: HUSHing up heterochromatin in human cells.
  5. findings demonstrate MPP8 and SIRT1 reciprocally regulate each other's function at multiple molecular layers through physical interaction; disruption of MPP8-SIRT1 interaction de-represses E cadherin expression and reduces cell motility and invasiveness suggesting this interplay plays critical role in MPP8- and SIRT1-mediated epithelial-mesenchymal transition
    Title: MPP8 and SIRT1 crosstalk in E-cadherin gene silencing and epithelial-mesenchymal transition.
  6. this study identified the HUSH (human silencing hub) complex, comprising three poorly characterized proteins, TASOR, MPP8, and periphilin; this complex is absent from Drosophila but is conserved from fish to humans.
    Title: GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.
  7. Knockdown analysis of MPP8 in HeLa cells resulted in derepression of a set of genes that are normally expressed in spermatogonia, spermatids and mature sperm, thereby indicating a role for this molecule in silencing testis-related genes in somatic cells.
    Title: Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis.
  8. interaction of HN with MPP8 may play a role in oncogenesis because MPP8 increases tumor cell motility and invasion
    Title: Humanin binds MPP8: mapping interaction sites of the peptide and protein.
  9. these results indicate that the chromatin association of MPP8 is regulated by Cdk-dependent phosphorylation.
    Title: Mitotic phosphorylation of MPP8 by cyclin-dependent kinases regulates chromatin dissociation.
  10. These findings reveal the molecular mechanism of selective binding of human MPP8 chromodomain to methylated histone H3K9.
    Title: Structural basis for specific binding of human MPP8 chromodomain to histone H3 methylated at lysine 9.
Submit: New GeneRIF Correction See all GeneRIFs (12)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • FLJ35237

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables chromatin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables chromatin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables methylated histone binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methylated histone binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in negative regulation of DNA-templated transcription IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of gene expression, epigenetic IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of single stranded viral RNA replication via double stranded DNA intermediate IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of DNA methylation-dependent heterochromatin formation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of DNA methylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of DNA methylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
  
part_of heterochromatin IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of heterochromatin IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleolus IDA
Inferred from Direct Assay
more info
  
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
part_of nucleosome IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
  

General protein information

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Preferred Names
M-phase phosphoprotein 8
Names
M-phase phosphoprotein, mpp
M-phase phosphoprotein, mpp8
two hybrid-associated protein 3 with RanBPM
NP_059990.2
XP_047286351.1
XP_047286352.1
XP_047286353.1
XP_054230584.1
XP_054230585.1
XP_054230586.1
XP_054230587.1
XP_054230588.1
XP_054230589.1
XP_054230590.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_017520.4 → NP_059990.2  M-phase phosphoprotein 8

    See identical proteins and their annotated locations for NP_059990.2

    Status: VALIDATED

    Source sequence(s)
    AL354808, BC003542, DA672302, R60056
    Consensus CDS
    CCDS9287.1
    UniProtKB/Swiss-Prot
    B7Z6F9, Q5JPE5, Q5JTQ0, Q86TK3, Q96MK4, Q99549, Q9BTP1
    Related
    ENSP00000355388.4, ENST00000361479.10
    Conserved Domains (4) summary
    cd00024
    Location:57 → 109
    CHROMO; Chromatin organization modifier (chromo) domain is a conserved region of around 50 amino acids found in a variety of chromosomal proteins, which appear to play a role in the functional organization of the eukaryotic nucleus. Experimental evidence ...
    cd00204
    Location:595 → 719
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    pfam12796
    Location:606 → 695
    Ank_2; Ankyrin repeats (3 copies)
    sd00045
    Location:600 → 631
    ANK; ANK repeat [structural motif]

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000013.11 Reference GRCh38.p14 Primary Assembly

    Range
    19633659..19673441
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047430395.1 → XP_047286351.1  M-phase phosphoprotein 8 isoform X1

  2. XM_047430396.1 → XP_047286352.1  M-phase phosphoprotein 8 isoform X2

  3. XM_047430397.1 → XP_047286353.1  M-phase phosphoprotein 8 isoform X3

    UniProtKB/TrEMBL
    B3KS10

RNA

  1. XR_007063684.1 RNA Sequence

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060937.1 Alternate T2T-CHM13v2.0

    Range
    18797911..18869507
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054374614.1 → XP_054230589.1  M-phase phosphoprotein 8 isoform X4

    UniProtKB/Swiss-Prot
    B7Z6F9, Q5JPE5, Q5JTQ0, Q86TK3, Q96MK4, Q99549, Q9BTP1

  2. XM_054374612.1 → XP_054230587.1  M-phase phosphoprotein 8 isoform X1

  3. XM_054374615.1 → XP_054230590.1  M-phase phosphoprotein 8 isoform X3

    UniProtKB/TrEMBL
    B3KS10

  4. XM_054374609.1 → XP_054230584.1  M-phase phosphoprotein 8 isoform X1

  5. XM_054374610.1 → XP_054230585.1  M-phase phosphoprotein 8 isoform X1

  6. XM_054374611.1 → XP_054230586.1  M-phase phosphoprotein 8 isoform X1

  7. XM_054374613.1 → XP_054230588.1  M-phase phosphoprotein 8 isoform X2

RNA

  1. XR_008488767.1 RNA Sequence

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q99549.2 GenPept UniProtKB/Swiss-Prot:Q99549

Gene LinkOut

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