Sign in to NCBI
Format

Send to:

Choose Destination

MPHOSPH8 M-phase phosphoprotein 8 [ Homo sapiens (human) ]

Gene ID: 54737, updated on 1-Jun-2020

Summary

Go to the top of the page Help
Official Symbol
MPHOSPH8provided by HGNC
Official Full Name
M-phase phosphoprotein 8provided by HGNC
Primary source
HGNC:HGNC:29810
See related
Ensembl:ENSG00000196199 MIM:611626
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
TWA3; mpp8; HSMPP8
Expression
Ubiquitous expression in ovary (RPKM 20.0), spleen (RPKM 15.8) and 25 other tissues See more
Orthologs
mouse all

Genomic context

Go to the top of the page Help
See MPHOSPH8 in Genome Data Viewer
Location:
13q12.11
Exon count:
16
Annotation release Status Assembly Chr Location
109.20200522 current GRCh38.p13 (GCF_000001405.39) 13 NC_000013.11 (19633648..19673441)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 13 NC_000013.10 (20207787..20247599)

Chromosome 13 - NC_000013.11Genomic Context describing neighboring genes Neighboring gene transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 Neighboring gene mitochondrial ribosomal protein S31 pseudogene 2 Neighboring gene cancer susceptibility 4 pseudogene 1 Neighboring gene estrogen-related receptor alpha pseudogene 1 Neighboring gene long intergenic non-protein coding RNA 350 Neighboring gene paraspeckle component 1 Neighboring gene RNA, 7SL, cytoplasmic 166, pseudogene Neighboring gene ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 4 pseudogene 1

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Humanin binds MPP8: mapping interaction sites of the peptide and protein. Maximov VV, et al. J Pept Sci, 2013 May. PMID 23532874
  2. Mitotic phosphorylation of MPP8 by cyclin-dependent kinases regulates chromatin dissociation. Nishigaki M, et al. Biochem Biophys Res Commun, 2013 Mar 22. PMID 23416073
  3. Structural basis for specific binding of human MPP8 chromodomain to histone H3 methylated at lysine 9. Li J, et al. PLoS One, 2011. PMID 22022377, Free PMC Article
  4. Structural insights for MPP8 chromodomain interaction with histone H3 lysine 9: potential effect of phosphorylation on methyl-lysine binding. Chang Y, et al. J Mol Biol, 2011 May 20. PMID 21419134, Free PMC Article
  5. Knockdown of MPP8 suppresses cell proliferation via regulation of HOXA5 in non-small cell lung cancer cells. Gao XY, et al. Cell Mol Biol (Noisy-le-grand), 2018 Jan 31. PMID 29412790

See all (37) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The study demonstrated that MPP8 was associated with non-small cell lung cancer cell proliferation through regulation of HOXA5.
    Title: Knockdown of MPP8 suppresses cell proliferation via regulation of HOXA5 in non-small cell lung cancer cells.
  2. The haploid screen identified HUSH, an epigenetic heterochromatin repressor complex composed of three subunits, TASOR, MPP8 and Periphilin. (Review)
    Title: Position-effect variegation revisited: HUSHing up heterochromatin in human cells.
  3. findings demonstrate MPP8 and SIRT1 reciprocally regulate each other's function at multiple molecular layers through physical interaction; disruption of MPP8-SIRT1 interaction de-represses E cadherin expression and reduces cell motility and invasiveness suggesting this interplay plays critical role in MPP8- and SIRT1-mediated epithelial-mesenchymal transition
    Title: MPP8 and SIRT1 crosstalk in E-cadherin gene silencing and epithelial-mesenchymal transition.
  4. this study identified the HUSH (human silencing hub) complex, comprising three poorly characterized proteins, TASOR, MPP8, and periphilin; this complex is absent from Drosophila but is conserved from fish to humans.
    Title: GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.
  5. Knockdown analysis of MPP8 in HeLa cells resulted in derepression of a set of genes that are normally expressed in spermatogonia, spermatids and mature sperm, thereby indicating a role for this molecule in silencing testis-related genes in somatic cells.
    Title: Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis.
  6. interaction of HN with MPP8 may play a role in oncogenesis because MPP8 increases tumor cell motility and invasion
    Title: Humanin binds MPP8: mapping interaction sites of the peptide and protein.
  7. these results indicate that the chromatin association of MPP8 is regulated by Cdk-dependent phosphorylation.
    Title: Mitotic phosphorylation of MPP8 by cyclin-dependent kinases regulates chromatin dissociation.
  8. These findings reveal the molecular mechanism of selective binding of human MPP8 chromodomain to methylated histone H3K9.
    Title: Structural basis for specific binding of human MPP8 chromodomain to histone H3 methylated at lysine 9.
  9. We determined the crystal structure of MPP8 chromodomain in complex with H3K9me3 peptide. MPP8 interacts with at least six histone H3 residues from glutamine 5 to serine 10.
    Title: Structural insights for MPP8 chromodomain interaction with histone H3 lysine 9: potential effect of phosphorylation on methyl-lysine binding.
  10. MPP8 mediates E-cadherin gene silencing and promotes breast tumour cell motility and invasion.
    Title: Methyl-H3K9-binding protein MPP8 mediates E-cadherin gene silencing and promotes tumour cell motility and invasion.
Submit: New GeneRIF Correction

Phenotypes

Go to the top of the page Help

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

Go to the top of the page Help

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Clone Names

  • FLJ35237

Gene Ontology Provided by GOA

Function Evidence Code Pubs
chromatin binding IDA
Inferred from Direct Assay
more info
 PubMed 
methylated histone binding IDA
Inferred from Direct Assay
more info
 PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
negative regulation of gene expression, epigenetic IDA
Inferred from Direct Assay
more info
 PubMed 
negative regulation of single stranded viral RNA replication via double stranded DNA intermediate IMP
Inferred from Mutant Phenotype
more info
 PubMed 
negative regulation of transcription, DNA-templated IMP
Inferred from Mutant Phenotype
more info
 PubMed 
positive regulation of methylation-dependent chromatin silencing IDA
Inferred from Direct Assay
more info
 PubMed 
regulation of DNA methylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
cytosol IDA
Inferred from Direct Assay
more info
  
heterochromatin IDA
Inferred from Direct Assay
more info
 PubMed 
nuclear heterochromatin IDA
Inferred from Direct Assay
more info
 PubMed 
nuclear nucleosome IDA
Inferred from Direct Assay
more info
 PubMed 
nucleolus IDA
Inferred from Direct Assay
more info
  
nucleoplasm IDA
Inferred from Direct Assay
more info
  
nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
plasma membrane IDA
Inferred from Direct Assay
more info
  

General protein information

Go to the top of the page Help
Preferred Names
M-phase phosphoprotein 8
Names
M-phase phosphoprotein, mpp
M-phase phosphoprotein, mpp8
two hybrid-associated protein 3 with RanBPM
NP_059990.2
XP_011533426.1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_017520.4 → NP_059990.2  M-phase phosphoprotein 8

    See identical proteins and their annotated locations for NP_059990.2

    Status: VALIDATED

    Source sequence(s)
    AL354808, BC003542, DA672302, R60056
    Consensus CDS
    CCDS9287.1
    UniProtKB/Swiss-Prot
    Q99549
    Related
    ENSP00000355388.4, ENST00000361479.10
    Conserved Domains (4) summary
    cd00024
    Location:57 → 109
    CHROMO; Chromatin organization modifier (chromo) domain is a conserved region of around 50 amino acids found in a variety of chromosomal proteins, which appear to play a role in the functional organization of the eukaryotic nucleus. Experimental evidence ...
    cd00204
    Location:595 → 719
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    pfam12796
    Location:606 → 695
    Ank_2; Ankyrin repeats (3 copies)
    sd00045
    Location:600 → 631
    ANK; ANK repeat [structural motif]

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000013.11 Reference GRCh38.p13 Primary Assembly

    Range
    19633648..19673441
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011535124.3 → XP_011533426.1  M-phase phosphoprotein 8 isoform X1

    Conserved Domains (4) summary
    cd00024
    Location:57 → 109
    CHROMO; Chromatin organization modifier (chromo) domain is a conserved region of around 50 amino acids found in a variety of chromosomal proteins, which appear to play a role in the functional organization of the eukaryotic nucleus. Experimental evidence ...
    cd00204
    Location:595 → 719
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    pfam12796
    Location:606 → 695
    Ank_2; Ankyrin repeats (3 copies)
    sd00045
    Location:600 → 631
    ANK; ANK repeat [structural motif]

RNA

  1. XR_001749586.2 RNA Sequence

  2. XR_001749585.2 RNA Sequence

  3. XR_001749584.2 RNA Sequence

  4. XR_002957453.1 RNA Sequence

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q99549.2 GenPept UniProtKB/Swiss-Prot:Q99549

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous
You are here: NCBI > Genes & Expression > Gene
Support Center