Enpp3 ectonucleotide pyrophosphatase/phosphodiesterase 3 [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Enpp3provided by RGD
Full Name: ectonucleotide pyrophosphatase/phosphodiesterase 3provided by RGD
Primary source: RGD:708511
See related: Ensembl:ENSRNOG00000013791 AllianceGenome:RGD:708511
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: B10; Npp3; RB13-6; E-NPP 3; PD-Ibeta
Summary: Predicted to enable metal ion binding activity; phosphodiesterase I activity; and pyrophosphatase activity. Predicted to be involved in several processes, including negative regulation of mast cell activation involved in immune response; negative regulation of mast cell proliferation; and nucleoside phosphate metabolic process. Predicted to be located in external side of plasma membrane and perinuclear region of cytoplasm. Predicted to be active in plasma membrane. Orthologous to human ENPP3 (ectonucleotide pyrophosphatase/phosphodiesterase 3). [provided by Alliance of Genome Resources, Jul 2025]
Expression: Biased expression in Liver (RPKM 270.4), Lung (RPKM 111.6) and 7 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1p12

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (22382717..22454324)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (20563700..20635044)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (21613148..21684483)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

NPP3 crystall structure at 2.77 A resolution reveals that the active-site zinc ions are missing and a large part of the active site and the surrounding residues are flexible.
Title: Crystallization of ectonucleotide phosphodiesterase/pyrophosphatase-3 and orientation of the SMB domains in the full-length ectodomain.
ecto-nucleotide pyrophosphatase/phosphodiesterase 1 and 3 are expressed on podocytes, but changes in expression of these enzymes are most likely not involved in etiology of insulin resistance in podocytes.
Title: Expression of membrane-bound NPP-type ecto-phosphodiesterases in rat podocytes cultured at normal and high glucose concentrations.
Lubrol resistance is an intrinsic property of NPP3 but not NPP1, which is acquired early during the biosynthetic process irrespective of its final destination, and depends on positively charged residues in its cytoplasmic tail.
Title: Differential detergent resistance of the apical and basolateral NPPases: relationship with polarized targeting.
The expression of NPP3 decreased during the aging mainly on cerebellum, hippocampus, and olfactory bulb.
Title: Expression mapping of ectonucleotide pyrophosphatase/phosphodiesterase 1-3 (E-NPP1-3) in different brain structures during rat development.

Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP diphosphatase activity	IEA Inferred from Electronic Annotation more info
enables GTP diphosphatase activity	IEA Inferred from Electronic Annotation more info
enables UTP diphosphatase activity	IEA Inferred from Electronic Annotation more info
enables bis(5'-adenosyl)-pentaphosphatase activity	ISO Inferred from Sequence Orthology more info
enables bis(5'-adenosyl)-pentaphosphatase activity	ISS Inferred from Sequence or Structural Similarity more info
enables bis(5'-adenosyl)-triphosphatase activity	IEA Inferred from Electronic Annotation more info
enables bis(5'-adenosyl)-triphosphatase activity	ISO Inferred from Sequence Orthology more info
enables bis(5'-adenosyl)-triphosphatase activity	ISS Inferred from Sequence or Structural Similarity more info
enables bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity	IEA Inferred from Electronic Annotation more info
enables bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity	ISO Inferred from Sequence Orthology more info
enables bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity	ISS Inferred from Sequence or Structural Similarity more info
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables cyclic-GMP-AMP hydrolase activity	ISS Inferred from Sequence or Structural Similarity more info
enables dinucleotide phosphatase activity	TAS Traceable Author Statement more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables nucleoside triphosphate diphosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables nucleoside triphosphate diphosphatase activity	IEA Inferred from Electronic Annotation more info
enables nucleoside triphosphate diphosphatase activity	ISO Inferred from Sequence Orthology more info
enables nucleoside triphosphate diphosphatase activity	ISS Inferred from Sequence or Structural Similarity more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables phosphodiesterase I activity	IEA Inferred from Electronic Annotation more info
enables phosphodiesterase I activity	ISO Inferred from Sequence Orthology more info
enables phosphodiesterase I activity	ISS Inferred from Sequence or Structural Similarity more info
enables phosphodiesterase I activity	TAS Traceable Author Statement more info	PubMed
enables pyrophosphatase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in ATP metabolic process	ISO Inferred from Sequence Orthology more info
involved_in ATP metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in basophil activation involved in immune response	ISO Inferred from Sequence Orthology more info
involved_in basophil activation involved in immune response	ISS Inferred from Sequence or Structural Similarity more info
NOT involved_in inorganic diphosphate transport	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cGAS/STING signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of inflammatory response	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of mast cell activation involved in immune response	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of mast cell activation involved in immune response	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of mast cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of mast cell proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in nucleoside triphosphate catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleoside triphosphate catabolic process	ISO Inferred from Sequence Orthology more info
involved_in phosphate ion homeostasis	ISO Inferred from Sequence Orthology more info
involved_in phosphate-containing compound metabolic process	ISO Inferred from Sequence Orthology more info
involved_in pyrimidine nucleotide metabolic process	ISO Inferred from Sequence Orthology more info
involved_in pyrimidine nucleotide metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of smooth muscle cell differentiation	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
located_in cell surface	IEA Inferred from Electronic Annotation more info
located_in cell surface	TAS Traceable Author Statement more info	PubMed
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in external side of plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: ectonucleotide pyrophosphatase/phosphodiesterase family member 3

Names: NPPase; RB13-6 antigen; alkaline phosphodiesterase; alkaline phosphodiesterase I; dinucleoside polyphosphatase; ectonucleotide pyrophosphatase/phosphodiesterase family member 3-like; nucleotide diphosphatase; nucleotide pyrophosphatase; phosphodiesterase I beta; phosphodiesterase I/nucleotide pyrophosphatase 3

NP_062243.2

EC 3.1.4.1

EC 3.6.1.9

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_019370.2 → NP_062243.2 ectonucleotide pyrophosphatase/phosphodiesterase family member 3

See identical proteins and their annotated locations for NP_062243.2

Status: PROVISIONAL

Source sequence(s)

BC097326

UniProtKB/Swiss-Prot

P70641, P97675, P97676, Q4V8L6, Q63490

UniProtKB/TrEMBL

A0A8L2QAC8, A6JUK4

Related

ENSRNOP00000018695.5, ENSRNOT00000018695.7

Conserved Domains (3) summary

smart00201
Location:95 → 138

SO; Somatomedin B -like domains

cd00091
Location:608 → 867

NUC; DNA/RNA non-specific endonuclease; prokaryotic and eukaryotic double- and single-stranded DNA and RNA endonucleases also present in phosphodiesterases. They exists as monomers and homodimers.

pfam01663
Location:162 → 486

Phosphodiest; Type I phosphodiesterase / nucleotide pyrophosphatase