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POLR2I RNA polymerase II subunit I [ Homo sapiens (human) ]

Gene ID: 5438, updated on 6-Sep-2022


Official Symbol
POLR2Iprovided by HGNC
Official Full Name
RNA polymerase II subunit Iprovided by HGNC
Primary source
See related
Ensembl:ENSG00000105258 MIM:180662; AllianceGenome:HGNC:9196
Gene type
protein coding
RefSeq status
Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
RPB9; hRPB14.5
This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq, Jul 2008]
Ubiquitous expression in testis (RPKM 23.3), brain (RPKM 15.2) and 25 other tissues See more
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Genomic context

See POLR2I in Genome Data Viewer
Exon count:
Annotation release Status Assembly Chr Location
110 current GRCh38.p14 (GCF_000001405.40) 19 NC_000019.10 (36113709..36114875, complement)
110 current T2T-CHM13v2.0 (GCF_009914755.1) 19 NC_060943.1 (38659527..38660693, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (36604611..36605777, complement)

Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC101927572 Neighboring gene THAP domain containing 8 Neighboring gene RNY5 pseudogene 10 Neighboring gene WD repeat domain 62 Neighboring gene ovo like zinc finger 3 Neighboring gene uncharacterized LOC105372385 Neighboring gene tubulin folding cofactor B

Genomic regions, transcripts, and products


  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of polymerase (RNA) II (DNA directed) polypeptide I (POLR2I, 14.5kDa) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env The transcription factors and kinases c-Jun, JNK, MEK, p38 MAPK, STAT-3, JAK-1, TFII D, TFII F, eIF-4E, and RNA polymerase II are induced by HIV-1 gp120 PubMed
Tat tat The interaction of Tip110 with HIV-1 Tat leads to a higher efficiency of elongation for RNAPII complexes formed on the LTR promoter PubMed
tat Expression of both Tip110 and Tat enhances phosphorylation of RNAPII at both serine 2 and serine 5, which account for Tip110 function in transcription activation PubMed
tat The interaction of Tip110 with HIV-1 Tat and the RNAPII C-terminal domain leads to the recruitment of increased CDK9/CycT1 to the transcription complex PubMed
tat HIV-1 Tat complexes with RNA polymerase II and cellular transcription factors as part of the HIV-1 transcription preinitiation and elongation complexes and stimulates RNA polymerase II processivity by inducing the phosphorylation of its C-terminal Domain PubMed
tat A small molecule compound C3 inhibits HIV-1 replication by suppressing HIV-1 Tat-mediated HIV-1 LTR-driven gene expression and phosphorylation of RNAPII through inhibition of Tat binding to CycT1 PubMed
tat JQ1, a small molecule inhibitor of Brd4, directly dissociates Brd4 from HIV-1 promoter and promotes subsequently HIV-1 Tat binding to HIV-1 promoter, resulting in increased phosphorylation of RNA polymerase II and viral transcription PubMed
tat HIV-1 Tat 41/44 peptide TAALS from the core domain of Tat inhibits Tat-mediated HIV-1 gene expression and replication by binding the Cdk2/Cyclin E complex and inhibiting the phosphorylation of serine 5 of RNAPII PubMed
Vpr vpr HIV-1 Vpr interacts with basal transcription complexes such as P-TEFb, TFIIH, TFIID (TBP) and TFIIB which activate RNA polymerase II gene transcription PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem


Products Interactant Other Gene Complex Source Pubs Description

General gene information



Gene Ontology Provided by GOA

Function Evidence Code Pubs
contributes_to DNA-directed 5'-3' RNA polymerase activity IBA
Inferred from Biological aspect of Ancestor
more info
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
enables protein binding IPI
Inferred from Physical Interaction
more info
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
Component Evidence Code Pubs
part_of RNA polymerase II, core complex IBA
Inferred from Biological aspect of Ancestor
more info
part_of RNA polymerase II, core complex IDA
Inferred from Direct Assay
more info
located_in nucleolus IEA
Inferred from Electronic Annotation
more info
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
located_in nucleoplasm TAS
Traceable Author Statement
more info
located_in nucleus IDA
Inferred from Direct Assay
more info

General protein information

Preferred Names
DNA-directed RNA polymerase II subunit RPB9
DNA-directed RNA polymerase II 14.5 kDa polypeptide
DNA-directed RNA polymerase II subunit I
RNA polymerase II 14.5 kDa subunit
RNA polymerase II subunit B9
polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
polymerase (RNA) II subunit I

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_006233.5NP_006224.1  DNA-directed RNA polymerase II subunit RPB9

    See identical proteins and their annotated locations for NP_006224.1

    Status: REVIEWED

    Source sequence(s)
    Consensus CDS
    P36954, Q6NW05
    ENSP00000221859.3, ENST00000221859.9
    Conserved Domains (2) summary
    RPB9; DNA-directed RNA polymerase, subunit M/Transcription elongation factor TFIIS [Transcription]
    Zn-ribbon_RPB9; C-terminal zinc ribbon domain of RPB9 subunit of RNA polymerase II

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 110 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly


  1. NC_000019.10 Reference GRCh38.p14 Primary Assembly

    36113709..36114875 complement
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0


  1. NC_060943.1 Alternate T2T-CHM13v2.0

    38659527..38660693 complement
    GenBank, FASTA, Sequence Viewer (Graphics)