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POLR2H RNA polymerase II subunit H [ Homo sapiens (human) ]

Gene ID: 5437, updated on 6-Jan-2019

Summary

Official Symbol
POLR2Hprovided by HGNC
Official Full Name
RNA polymerase II subunit Hprovided by HGNC
Primary source
HGNC:HGNC:9195
See related
Ensembl:ENSG00000163882 MIM:606023
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
RPB8; RPB17; RPABC3
Summary
The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
Expression
Ubiquitous expression in duodenum (RPKM 14.6), small intestine (RPKM 13.6) and 25 other tissues See more
Orthologs

Genomic context

See POLR2H in Genome Data Viewer
Location:
3q27.1
Exon count:
6
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 3 NC_000003.12 (184361710..184368595)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 3 NC_000003.11 (184079502..184086383)

Chromosome 3 - NC_000003.12Genomic Context describing neighboring genes Neighboring gene eukaryotic translation initiation factor 4 gamma 1 Neighboring gene small nucleolar RNA, C/D box 66 Neighboring gene family with sequence similarity 131 member A Neighboring gene chloride voltage-gated channel 2 Neighboring gene thrombopoietin Neighboring gene chordin

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of polymerase (RNA) II (DNA directed) polypeptide H (POLR2H) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env The transcription factors and kinases c-Jun, JNK, MEK, p38 MAPK, STAT-3, JAK-1, TFII D, TFII F, eIF-4E, and RNA polymerase II are induced by HIV-1 gp120 PubMed
Tat tat The interaction of Tip110 with HIV-1 Tat leads to a higher efficiency of elongation for RNAPII complexes formed on the LTR promoter PubMed
tat Expression of both Tip110 and Tat enhances phosphorylation of RNAPII at both serine 2 and serine 5, which account for Tip110 function in transcription activation PubMed
tat The interaction of Tip110 with HIV-1 Tat and the RNAPII C-terminal domain leads to the recruitment of increased CDK9/CycT1 to the transcription complex PubMed
tat HIV-1 Tat complexes with RNA polymerase II and cellular transcription factors as part of the HIV-1 transcription preinitiation and elongation complexes and stimulates RNA polymerase II processivity by inducing the phosphorylation of its C-terminal Domain PubMed
tat A small molecule compound C3 inhibits HIV-1 replication by suppressing HIV-1 Tat-mediated HIV-1 LTR-driven gene expression and phosphorylation of RNAPII through inhibition of Tat binding to CycT1 PubMed
tat JQ1, a small molecule inhibitor of Brd4, directly dissociates Brd4 from HIV-1 promoter and promotes subsequently HIV-1 Tat binding to HIV-1 promoter, resulting in increased phosphorylation of RNA polymerase II and viral transcription PubMed
tat HIV-1 Tat upregulates the transcription by RNA polymerase III of co-transfected or endogenous cellular Alu-repeated sequences by activating transcription factor TFIIIC PubMed
Vpr vpr HIV-1 Vpr interacts with basal transcription complexes such as P-TEFb, TFIIH, TFIID (TBP) and TFIIB which activate RNA polymerase II gene transcription PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
contributes_to RNA polymerase I activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
contributes_to RNA polymerase II activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
contributes_to RNA polymerase III activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
single-stranded DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
Component Evidence Code Pubs
RNA polymerase I complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
RNA polymerase II, core complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
RNA polymerase II, core complex IDA
Inferred from Direct Assay
more info
PubMed 
RNA polymerase III complex IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
protein-DNA complex IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Names
DNA-directed RNA polymerase II subunit H
DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide
RPB8 homolog
polymerase (RNA) II (DNA directed) polypeptide H
polymerase (RNA) II subunit H
NP_001265627.1
NP_001265628.1
NP_001265629.1
NP_001265643.1
NP_001265644.1
NP_001333808.1
NP_006223.2
XP_006713729.1
XP_006713730.1
XP_016862125.1
XP_016862126.1
XP_024309370.1
XP_024309371.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001278698.1NP_001265627.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 1

    See identical proteins and their annotated locations for NP_001265627.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
    Source sequence(s)
    AA431357, BG529732, BX283032, CN368789, Z49199
    Consensus CDS
    CCDS63859.1
    UniProtKB/Swiss-Prot
    P52434
    Related
    ENSP00000415536.1, ENST00000429568.1
    Conserved Domains (1) summary
    smart00658
    Location:2106
    RPOL8c; RNA polymerase subunit 8
  2. NM_001278699.2NP_001265628.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 3

    See identical proteins and their annotated locations for NP_001265628.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR, uses a downstream start codon and uses an alternate splice site in the 3' coding region, resulting in a frameshift, compared to variant 1. Variants 3 and 4 encode the same isoform (3), which is shorter and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AC078797
    Consensus CDS
    CCDS63861.1
    UniProtKB/Swiss-Prot
    P52434
    Conserved Domains (1) summary
    smart00658
    Location:1111
    RPOL8c; RNA polymerase subunit 8
  3. NM_001278700.1NP_001265629.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 3

    See identical proteins and their annotated locations for NP_001265629.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) contains an alternate 5' terminal exon, initiates translation at a downstream start codon and uses an alternate splice site in the 3' coding region, resulting in a frameshift, compared to variant 1. Variants 3 and 4 encode the same isoform (3), which is shorter and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AA431357, BM541682, BX283032, HY178178, Z49199
    Consensus CDS
    CCDS63861.1
    UniProtKB/Swiss-Prot
    P52434
    Related
    ENSP00000398622.1, ENST00000438240.5
    Conserved Domains (1) summary
    smart00658
    Location:1111
    RPOL8c; RNA polymerase subunit 8
  4. NM_001278714.1NP_001265643.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 4

    See identical proteins and their annotated locations for NP_001265643.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 5' coding region and uses an alternate splice site in the 3' coding region, resulting in a frameshift, compared to variant 1. It encodes isoform 4, which is shorter and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AI244238, BQ065952, BX283032, CN368789
    Consensus CDS
    CCDS63860.1
    UniProtKB/Swiss-Prot
    P52434
    Related
    ENSP00000411883.1, ENST00000430783.5
    Conserved Domains (1) summary
    smart00658
    Location:2119
    RPOL8c; RNA polymerase subunit 8
  5. NM_001278715.1NP_001265644.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 5

    See identical proteins and their annotated locations for NP_001265644.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (6) differs in the 5' UTR and has multiple coding region differences compared to variant 1, one of which results in a frameshift. It encodes isoform 5 which is shorter and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AA431357, BI603667, BQ065952, BX283032, CN368789, Z49199
    Consensus CDS
    CCDS63862.1
    UniProtKB/Swiss-Prot
    P52434
    Related
    ENSP00000393773.1, ENST00000443489.5
    Conserved Domains (1) summary
    cl02716
    Location:183
    RNA_pol_Rpb8; RNA polymerase Rpb8
  6. NM_001346879.1NP_001333808.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 6

    Status: REVIEWED

    Source sequence(s)
    AC078797
    Conserved Domains (1) summary
    cl02716
    Location:170
    RNA_pol_Rpb8; RNA polymerase Rpb8
  7. NM_006232.4NP_006223.2  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 2

    See identical proteins and their annotated locations for NP_006223.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AC078797
    Consensus CDS
    CCDS3264.1
    UniProtKB/Swiss-Prot
    P52434
    Related
    ENSP00000392913.1, ENST00000456318.5
    Conserved Domains (1) summary
    smart00658
    Location:2147
    RPOL8c; RNA polymerase subunit 8

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000003.12 Reference GRCh38.p12 Primary Assembly

    Range
    184361710..184368595
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006713666.3XP_006713729.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform X1

    See identical proteins and their annotated locations for XP_006713729.1

    UniProtKB/Swiss-Prot
    P52434
    Conserved Domains (1) summary
    smart00658
    Location:2106
    RPOL8c; RNA polymerase subunit 8
  2. XM_017006636.2XP_016862125.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform X3

    UniProtKB/Swiss-Prot
    P52434
    Conserved Domains (1) summary
    smart00658
    Location:2119
    RPOL8c; RNA polymerase subunit 8
  3. XM_017006637.1XP_016862126.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform X5

    UniProtKB/Swiss-Prot
    P52434
    Conserved Domains (1) summary
    cl02716
    Location:183
    RNA_pol_Rpb8; RNA polymerase Rpb8
  4. XM_006713667.2XP_006713730.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform X1

    See identical proteins and their annotated locations for XP_006713730.1

    UniProtKB/Swiss-Prot
    P52434
    Conserved Domains (1) summary
    smart00658
    Location:2106
    RPOL8c; RNA polymerase subunit 8
  5. XM_024453603.1XP_024309371.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform X4

    Related
    ENSP00000399882.1, ENST00000452961.5
    Conserved Domains (1) summary
    smart00658
    Location:1111
    RPOL8c; RNA polymerase subunit 8
  6. XM_024453602.1XP_024309370.1  DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform X2

    Conserved Domains (1) summary
    cl02716
    Location:170
    RNA_pol_Rpb8; RNA polymerase Rpb8
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