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Mid1 midline 1 [ Rattus norvegicus (Norway rat) ]

Gene ID: 54252, updated on 7-Apr-2025

Summary

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Official Symbol
Mid1provided by RGD
Official Full Name
midline 1provided by RGD
Primary source
RGD:2640
See related
EnsemblRapid:ENSRNOG00000003613 AllianceGenome:RGD:2640
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
Fxy; Midline1
Summary
Predicted to enable several functions, including microtubule binding activity; protein homodimerization activity; and ubiquitin protein ligase binding activity. Predicted to be involved in positive regulation of stress-activated MAPK cascade; protein localization to microtubule; and regulation of microtubule cytoskeleton organization. Predicted to act upstream of or within negative regulation of microtubule depolymerization. Predicted to be located in Golgi apparatus; cytosol; and microtubule cytoskeleton. Predicted to be active in cytoplasm. Human ortholog(s) of this gene implicated in Opitz GBBB syndrome. Orthologous to human MID1 (midline 1). [provided by Alliance of Genome Resources, Apr 2025]
Expression
Biased expression in Muscle (RPKM 71.3), Kidney (RPKM 61.8) and 9 other tissues See more
Orthologs
human mouse all
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Genomic context

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See Mid1 in Genome Data Viewer
Location:
Xq13
Exon count:
14
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) X NC_086039.1 (27678248..28053049, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) X NC_051356.1 (24116674..24491205, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) X NC_005120.4 (25458782..25839941, complement)

Chromosome X - NC_086039.1Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC120099331 Neighboring gene chloride voltage-gated channel 4 Neighboring gene uncharacterized LOC134484072 Neighboring gene capping actin protein of muscle Z-line subunit alpha 1, pseudogene 1 Neighboring gene Rho GTPase activating protein 6 Neighboring gene holocytochrome c synthase Neighboring gene amelogenin, X-linked

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. Alternative polyadenylation signals and promoters act in concert to control tissue-specific expression of the Opitz Syndrome gene MID1. Winter J, et al. BMC Mol Biol, 2007 Nov 15. PMID 18005432, Free PMC Article
  2. Evolutionary rate of a gene affected by chromosomal position. Perry J, et al. Curr Biol, 1999 Sep 9. PMID 10508587
  3. Active transport of the ubiquitin ligase MID1 along the microtubules is regulated by protein phosphatase 2A. Aranda-Orgillés B, et al. PLoS One, 2008. PMID 18949047, Free PMC Article
  4. Widely spaced alternative promoters, conserved between human and rodent, control expression of the Opitz syndrome gene MID1. Landry JR, et al. Genomics, 2002 Nov. PMID 12408967
  5. Mig12, a novel Opitz syndrome gene product partner, is expressed in the embryonic ventral midline and co-operates with Mid1 to bundle and stabilize microtubules. Berti C, et al. BMC Cell Biol, 2004 Feb 29. PMID 15070402, Free PMC Article

See all (11) citations in PubMed

GeneRIFs: Gene References Into Functions

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables enzyme binding ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables microtubule binding ISO
Inferred from Sequence Orthology
more info
  
enables phosphoprotein binding ISO
Inferred from Sequence Orthology
more info
  
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase binding ISO
Inferred from Sequence Orthology
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within negative regulation of microtubule depolymerization ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of stress-activated MAPK cascade ISO
Inferred from Sequence Orthology
more info
  
involved_in protein localization to microtubule ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of microtubule cytoskeleton organization IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in Golgi apparatus IEA
Inferred from Electronic Annotation
more info
  
located_in Golgi apparatus ISO
Inferred from Sequence Orthology
more info
  
located_in centriolar satellite IEA
Inferred from Electronic Annotation
more info
  
located_in centriolar satellite ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in cytoplasmic microtubule ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in microtubule IEA
Inferred from Electronic Annotation
more info
  
located_in microtubule ISO
Inferred from Sequence Orthology
more info
  
located_in microtubule cytoskeleton ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
E3 ubiquitin-protein ligase Midline-1
Names
Finger on X and Y (in rat only on X)
RING finger protein Midline-1
RING-type E3 ubiquitin transferase Midline-1
midline 1 (Opitz/BBB syndrome)
tripartite motif-containing protein 18
NP_075216.1
XP_006256887.1
XP_017457631.1
XP_017457632.1
XP_038955925.1
XP_038955926.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001431605.1 → NP_001418534.1  E3 ubiquitin-protein ligase Midline-1 isoform 1

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000021
    UniProtKB/Swiss-Prot
    P82458
    UniProtKB/TrEMBL
    A6K2D8

  2. NM_001431606.1 → NP_001418535.1  E3 ubiquitin-protein ligase Midline-1 isoform 1

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000021
    UniProtKB/Swiss-Prot
    P82458
    UniProtKB/TrEMBL
    A6K2D8

  3. NM_001431607.1 → NP_001418536.1  E3 ubiquitin-protein ligase Midline-1 isoform 1

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000021
    UniProtKB/Swiss-Prot
    P82458
    UniProtKB/TrEMBL
    A6K2D8

  4. NM_001431608.1 → NP_001418537.1  E3 ubiquitin-protein ligase Midline-1 isoform 1

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000021
    UniProtKB/Swiss-Prot
    P82458
    UniProtKB/TrEMBL
    A6K2D8

  5. NM_001431609.1 → NP_001418538.1  E3 ubiquitin-protein ligase Midline-1 isoform 2

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000021

  6. NM_022927.2 → NP_075216.1  E3 ubiquitin-protein ligase Midline-1 isoform 1

    See identical proteins and their annotated locations for NP_075216.1

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000021
    UniProtKB/Swiss-Prot
    P82458
    UniProtKB/TrEMBL
    A6K2D8
    Related
    ENSRNOP00000004873.5, ENSRNOT00000004873.8
    Conserved Domains (7) summary
    smart00502
    Location:219 → 344
    BBC; B-Box C-terminal domain
    cd00063
    Location:381 → 481
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cd16753
    Location:8 → 61
    RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins
    cd19822
    Location:168 → 214
    Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins
    cd19836
    Location:115 → 164
    Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins
    pfam18568
    Location:323 → 374
    COS; TRIM C-terminal subgroup One Signature domain
    cl02614
    Location:485 → 661
    SPRY; SPRY domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086039.1 Reference GRCr8

    Range
    27678248..28053049 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017602142.3 → XP_017457631.1  E3 ubiquitin-protein ligase Midline-1 isoform X1

    Conserved Domains (7) summary
    smart00502
    Location:219 → 344
    BBC; B-Box C-terminal domain
    cd00063
    Location:381 → 481
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cd16753
    Location:8 → 61
    RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins
    cd19822
    Location:168 → 214
    Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins
    cd19836
    Location:115 → 164
    Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins
    pfam18568
    Location:323 → 374
    COS; TRIM C-terminal subgroup One Signature domain
    cl02614
    Location:485 → 661
    SPRY; SPRY domain

  2. XM_039099997.2 → XP_038955925.1  E3 ubiquitin-protein ligase Midline-1 isoform X1

    Conserved Domains (7) summary
    smart00502
    Location:219 → 344
    BBC; B-Box C-terminal domain
    cd00063
    Location:381 → 481
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cd16753
    Location:8 → 61
    RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins
    cd19822
    Location:168 → 214
    Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins
    cd19836
    Location:115 → 164
    Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins
    pfam18568
    Location:323 → 374
    COS; TRIM C-terminal subgroup One Signature domain
    cl02614
    Location:485 → 661
    SPRY; SPRY domain

  3. XM_017602143.3 → XP_017457632.1  E3 ubiquitin-protein ligase Midline-1 isoform X1

    Conserved Domains (7) summary
    smart00502
    Location:219 → 344
    BBC; B-Box C-terminal domain
    cd00063
    Location:381 → 481
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cd16753
    Location:8 → 61
    RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins
    cd19822
    Location:168 → 214
    Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins
    cd19836
    Location:115 → 164
    Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins
    pfam18568
    Location:323 → 374
    COS; TRIM C-terminal subgroup One Signature domain
    cl02614
    Location:485 → 661
    SPRY; SPRY domain

  4. XM_039099998.1 → XP_038955926.1  E3 ubiquitin-protein ligase Midline-1 isoform X2

    Related
    ENSRNOP00000101804.1, ENSRNOT00000141079.1
    Conserved Domains (6) summary
    smart00502
    Location:182 → 306
    BBC; B-Box C-terminal domain
    cd00063
    Location:343 → 443
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cd16753
    Location:8 → 61
    RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins
    cd19836
    Location:115 → 164
    Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins
    pfam18568
    Location:285 → 336
    COS; TRIM C-terminal subgroup One Signature domain
    cl02614
    Location:447 → 623
    SPRY; SPRY domain

  5. XM_006256825.5 → XP_006256887.1  E3 ubiquitin-protein ligase Midline-1 isoform X1

    Conserved Domains (7) summary
    smart00502
    Location:219 → 344
    BBC; B-Box C-terminal domain
    cd00063
    Location:381 → 481
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cd16753
    Location:8 → 61
    RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins
    cd19822
    Location:168 → 214
    Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins
    cd19836
    Location:115 → 164
    Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins
    pfam18568
    Location:323 → 374
    COS; TRIM C-terminal subgroup One Signature domain
    cl02614
    Location:485 → 661
    SPRY; SPRY domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P82458.1 GenPept UniProtKB/Swiss-Prot:P82458

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