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LOC541812 chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic [ Zea mays ]

Gene ID: 541812, updated on 23-Feb-2026
Gene symbol
LOC541812
Gene description
chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic
Locus tag
ZEAMMB73_Zm00001d008559
See related
EnsemblRapid:Zm00001eb335300
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Zea mays
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea
Also known as
CRS2; GRMZM2G132021
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Try the new Transcripts and proteins table
See LOC541812 in Genome Data Viewer
Location:
chromosome: 8
Exon count:
10
Annotation release Status Assembly Chr Location
RS_2025_02 current Zm-B73-REFERENCE-NAM-5.0 (GCF_902167145.1) 8 NC_050103.1 (13011116..13019358)

Chromosome 8 - NC_050103.1Genomic Context describing neighboring genes Neighboring gene CDP-diacylglycerol--serine O-phosphatidyltransferase 2 Neighboring gene uncharacterized LOC103634796 Neighboring gene putative cinnamyl-alcohol dehydrogenase family protein pseudogene Neighboring gene zinc finger protein GIS3

Genomic Sequence:
NC_050103.1 Chromosome 8 Reference Zm-B73-REFERENCE-NAM-5.0 Primary Assembly

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Structural analysis of the group II intron splicing factor CRS2 yields insights into its protein and RNA interaction surfaces. Ostheimer GJ, et al. J Mol Biol, 2005 Jan 7. PMID 15567410
  2. Recruitment of a peptidyl-tRNA hydrolase as a facilitator of group II intron splicing in chloroplasts. Jenkins BD, et al. EMBO J, 2001 Feb 15. PMID 11179231, Free PMC Article
  3. Nuclear mutations that block group II RNA splicing in maize chloroplasts reveal several intron classes with distinct requirements for splicing factors. Jenkins BD, et al. Plant Cell, 1997 Mar. PMID 9090875, Free PMC Article
  4. Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants. Vogel J, et al. Nucleic Acids Res, 1999 Oct 1. PMID 10481026, Free PMC Article
  5. Formation of the CRS2-CAF2 group II intron splicing complex is mediated by a 22-amino acid motif in the COOH-terminal region of CAF2. Ostheimer GJ, et al. J Biol Chem, 2006 Feb 24. PMID 16379013

See all (10) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. a 22-amino acid motif in the COOH-terminal region of CAF2 mediates formation of the CRS2-CAF2 group II intron splicing complex
    Title: Formation of the CRS2-CAF2 group II intron splicing complex is mediated by a 22-amino acid motif in the COOH-terminal region of CAF2.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Gene Ontology Provided by RefSeq

Function Evidence Code Pubs
enables peptidyl-tRNA hydrolase activity IEA
Inferred from Electronic Annotation
more info
 PubMed 
Preferred Names
chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic
Names
chloroplast RNA splicing2
chloroplastic RNA splicing factor 2
protein CHLOROPLAST RNA SPLICING 2

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001111493.2 → NP_001104963.2  chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic precursor

    Status: VALIDATED

    Source sequence(s)
    BT036053, BT038431
    UniProtKB/Swiss-Prot
    Q9M5P4
    UniProtKB/TrEMBL
    B4FMU3
    Related
    Zm00001eb335300_P003, Zm00001eb335300_T003
    Conserved Domains (1) summary
    cd02406
    Location:60 → 250
    CRS2; Chloroplast RNA splicing 2 (CRS2) is a nuclear-encoded protein required for the splicing of group II introns in the chloroplast. CRS2 forms stable complexes with two CRS2-associated factors, CAF1 and CAF2, which are required for the splicing of distinct ...

RefSeqs of Annotated Genomes: GCF_902167145.1-RS_2025_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference Zm-B73-REFERENCE-NAM-5.0 Primary Assembly

Genomic

  1. NC_050103.1 Reference Zm-B73-REFERENCE-NAM-5.0 Primary Assembly

    Range
    13011116..13019358
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_020542318.2 → XP_020397907.1  chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X2

    UniProtKB/Swiss-Prot
    Q9M5P4
    UniProtKB/TrEMBL
    A0A804QK29
    Related
    Zm00001eb335300_P002, Zm00001eb335300_T002
    Conserved Domains (1) summary
    cl00352
    Location:60 → 218
    PTH; Peptidyl-tRNA hydrolase (PTH) is a monomeric protein that cleaves the ester bond linking the nascent peptide and tRNA when peptidyl-tRNA is released prematurely from the ribosome. This ensures the recycling of peptidyl-tRNAs into tRNAs produced through ...

  2. XM_020542320.2 → XP_020397909.1  chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X3

    UniProtKB/Swiss-Prot
    Q9M5P4
    Conserved Domains (1) summary
    cl00352
    Location:60 → 219
    PTH; Peptidyl-tRNA hydrolase (PTH) is a monomeric protein that cleaves the ester bond linking the nascent peptide and tRNA when peptidyl-tRNA is released prematurely from the ribosome. This ensures the recycling of peptidyl-tRNAs into tRNAs produced through ...

  3. XM_020542317.2 → XP_020397906.1  chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic isoform X1

    UniProtKB/Swiss-Prot
    Q9M5P4
    UniProtKB/TrEMBL
    B4FMU3
    Conserved Domains (1) summary
    cd02406
    Location:60 → 250
    CRS2; Chloroplast RNA splicing 2 (CRS2) is a nuclear-encoded protein required for the splicing of group II introns in the chloroplast. CRS2 forms stable complexes with two CRS2-associated factors, CAF1 and CAF2, which are required for the splicing of distinct ...

RNA

  1. XR_004851812.1 RNA Sequence

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9M5P4.1 GenPept UniProtKB/Swiss-Prot:Q9M5P4

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