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Folh1 folate hydrolase 1 [ Mus musculus (house mouse) ]

Gene ID: 53320, updated on 5-Jul-2025
Official Symbol
Folh1provided by MGI
Official Full Name
folate hydrolase 1provided by MGI
Primary source
MGI:MGI:1858193
See related
Ensembl:ENSMUSG00000001773 AllianceGenome:MGI:1858193
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
GCP2; mopsm
Summary
Enables carboxypeptidase activity and dipeptidase activity. Acts upstream of or within folic acid-containing compound metabolic process and proteolysis. Located in plasma membrane. Is expressed in central nervous system; male reproductive gland or organ; metanephros; and submandibular gland. Human ortholog(s) of this gene implicated in abdominal aortic aneurysm and cerebral infarction. Orthologous to several human genes including FOLH1 (folate hydrolase 1). [provided by Alliance of Genome Resources, Jul 2025]
Expression
Biased expression in kidney adult (RPKM 9.1), genital fat pad adult (RPKM 4.5) and 4 other tissues See more
Orthologs
human all
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See Folh1 in Genome Data Viewer
Location:
7 D3; 7 48.51 cM
Exon count:
20
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 7 NC_000073.7 (86365733..86425171, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 7 NC_000073.6 (86716525..86776053, complement)

Chromosome 7 - NC_000073.7Genomic Context describing neighboring genes Neighboring gene olfactory receptor family 14 subfamily C member 40 Neighboring gene olfactory receptor family 14 subfamily C member 39 Neighboring gene vomeronasal 2, receptor 77 Neighboring gene STARR-seq mESC enhancer starr_19465 Neighboring gene vomeronasal 2, receptor, pseudogene 87 Neighboring gene STARR-seq mESC enhancer starr_19466

Go to nucleotide: Graphics FASTA GenBank

  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Related articles in PubMed

  1. GCPII Inhibition Promotes Remyelination after Peripheral Nerve Injury in Aged Mice. Su Y, et al. Int J Mol Sci, 2024 Jun 23. PMID 39000003, Free PMC Article
  2. Distinguishing Plasmodium falciparum treatment failures from reinfections by restrictions fragment length polymorphism and polymerase chain reaction genotyping. Ohrt C, et al. Am J Trop Med Hyg, 1997 Oct. PMID 9347959
  3. Metabolite differences between glutamate carboxypeptidase II gene knockout mice and their wild-type littermates after traumatic brain injury: a 7-tesla (1)H-MRS study. Wu W, et al. BMC Neurosci, 2018 Nov 20. PMID 30458729, Free PMC Article
  4. A novel PSMA/GCPII-deficient mouse model shows enlarged seminal vesicles upon aging. Vorlová B, et al. Prostate, 2019 Feb. PMID 30256431
  5. Deletion of the glutamate carboxypeptidase II gene in mice reveals a second enzyme activity that hydrolyzes N-acetylaspartylglutamate. Bacich DJ, et al. J Neurochem, 2002 Oct. PMID 12358725

See all (35) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. GCPII Inhibition Promotes Remyelination after Peripheral Nerve Injury in Aged Mice.
    Title: GCPII Inhibition Promotes Remyelination after Peripheral Nerve Injury in Aged Mice.
  2. Parallel Metabolomics and Lipidomics of a PSMA/GCPII Deficient Mouse Model Reveal Alteration of NAAG Levels and Brain Lipid Composition.
    Title: Parallel Metabolomics and Lipidomics of a PSMA/GCPII Deficient Mouse Model Reveal Alteration of NAAG Levels and Brain Lipid Composition.
  3. Deletion of glutamate carboxypeptidase II (GCPII), but not GCPIII, provided long-term benefits in mice with traumatic brain injury.
    Title: Deletion of glutamate carboxypeptidase II (GCPII), but not GCPIII, provided long-term benefits in mice with traumatic brain injury.
  4. decrease in both glutamate and N-acetylaspartate levels in the ipsilateral hippocampus at 24 h post-traumatic brain injury (TBI); however, the reduction in glutamate and N-acetylaspartate levels was less marked in GCP II-KO mice than in WT mice. T2 MR data and brain water-content analysis demonstrated that the extent of cortical edema and brain swelling was less in KO than in WT mice after TBI .
    Title: Metabolite differences between glutamate carboxypeptidase II gene knockout mice and their wild-type littermates after traumatic brain injury: a 7-tesla (1)H-MRS study.
  5. Data support the hypothesis that the neuroprotective efficacy of glutamate carboxypeptidase II knockout in traumatic brain injury is mediated via a reduction in oxidative stress.
    Title: Glutamate carboxypeptidase II gene knockout attenuates oxidative stress and cortical apoptosis after traumatic brain injury.
  6. Knockout of the GCPII gene is not embryonic lethal and affords histopathological protection with improved long-term behavioral outcomes after traumatic brain injury
    Title: Mice lacking glutamate carboxypeptidase II develop normally, but are less susceptible to traumatic brain injury.
  7. Inhibition of glutamate carboxypeptidase II (GCPII) activity as a treatment for cognitive impairment in multiple sclerosis.
    Title: Inhibition of glutamate carboxypeptidase II (GCPII) activity as a treatment for cognitive impairment in multiple sclerosis.
  8. PSMA plays a VEGF-independent role in retinal angiogenesis
    Title: Prostate specific membrane antigen (PSMA) regulates angiogenesis independently of VEGF during ocular neovascularization.
  9. The combined effects of a haplo-insufficiency of glutamate carboxypeptidase II and dietary folic acid deficiency esult in reciprocal protection against cognitive and social deficits.
    Title: Glutamate carboxypeptidase II and folate deficiencies result in reciprocal protection against cognitive and social deficits in mice: implications for neurodevelopmental disorders.
  10. analysis of GCP2 and GCP3; beta-citrylglutamate hydrolase is glutamate carboxypeptidase 3
    Title: Molecular identification of β-citrylglutamate hydrolase as glutamate carboxypeptidase 3.
Submit: New GeneRIF Correction See all GeneRIFs (12)

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Homology

Clone Names

  • MGC141397

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables Ac-Asp-Glu binding IEA
Inferred from Electronic Annotation
more info
  
enables Ac-Asp-Glu binding ISO
Inferred from Sequence Orthology
more info
  
enables carboxypeptidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables carboxypeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables carboxypeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables dipeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables dipeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metallocarboxypeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables metallocarboxypeptidase activity ISO
Inferred from Sequence Orthology
more info
  
enables metallopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables peptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables peptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables tetrahydrofolyl-poly(glutamate) polymer binding IEA
Inferred from Electronic Annotation
more info
  
enables tetrahydrofolyl-poly(glutamate) polymer binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within folic acid-containing compound metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of apoptotic process ISO
Inferred from Sequence Orthology
more info
  
involved_in protein catabolic process ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within proteolysis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cell surface IEA
Inferred from Electronic Annotation
more info
  
located_in cell surface ISO
Inferred from Sequence Orthology
more info
  
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
  
Preferred Names
glutamate carboxypeptidase 2
Names
FGCP
GCPII
N-acetylated-alpha-linked acidic dipeptidase I
NAALADase I
folylpoly-gamma-glutamate carboxypeptidase
glutamate carboxypeptidase II
mGCP
membrane glutamate carboxypeptidase
prostate-specific membrane antigen homolog
pteroylpoly-gamma-glutamate carboxypeptidase
NP_001153178.1
NP_058050.3
XP_006508086.1
XP_011240163.1

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001159706.1 → NP_001153178.1  glutamate carboxypeptidase 2 isoform 2

    See identical proteins and their annotated locations for NP_001153178.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks an exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1.
    Source sequence(s)
    AK002920, BB179784, BB872570, BC119604, BC119605
    Consensus CDS
    CCDS52300.1
    UniProtKB/TrEMBL
    Q0VDM6
    Related
    ENSMUSP00000102892.4, ENSMUST00000107271.10
    Conserved Domains (3) summary
    cd08022
    Location:275 → 564
    M28_PSMA_like; M28 Zn-peptidase prostate-specific membrane antigen
    cd02121
    Location:122 → 365
    PA_GCPII_like; PA_GCPII_like: Protease-associated domain containing protein, glutamate carboxypeptidase II (GCPII)-like. This group contains various PA domain-containing proteins similar to GCPII including, GCPIII (NAALADase2) and NAALADase L. These proteins belong to ...
    pfam04253
    Location:601 → 716
    TFR_dimer; Transferrin receptor-like dimerization domain

  2. NM_016770.3 → NP_058050.3  glutamate carboxypeptidase 2 isoform 1

    See identical proteins and their annotated locations for NP_058050.3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AK002920, BB179784, BB872570, BC119605
    Consensus CDS
    CCDS21436.1
    UniProtKB/Swiss-Prot
    O35409, Q0VDM5, Q9DCC2
    Related
    ENSMUSP00000001824.6, ENSMUST00000001824.7
    Conserved Domains (4) summary
    cd08022
    Location:356 → 597
    M28_PSMA_like; M28 Zn-peptidase prostate-specific membrane antigen
    cd02121
    Location:122 → 348
    PA_GCPII_like; PA_GCPII_like: Protease-associated domain containing protein, glutamate carboxypeptidase II (GCPII)-like. This group contains various PA domain-containing proteins similar to GCPII including, GCPIII (NAALADase2) and NAALADase L. These proteins belong to ...
    pfam04253
    Location:634 → 749
    TFR_dimer; Transferrin receptor-like dimerization domain
    cl14876
    Location:70 → 116
    Zinc_peptidase_like; Zinc peptidases M18, M20, M28, and M42

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000073.7 Reference GRCm39 C57BL/6J

    Range
    86365733..86425171 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006508023.5 → XP_006508086.1  glutamate carboxypeptidase 2 isoform X1

    Conserved Domains (3) summary
    cd02121
    Location:122 → 306
    PA_GCPII_like; Protease-associated domain containing protein, glutamate carboxypeptidase II (GCPII)-like. This group contains various PA domain-containing proteins similar to GCPII including, GCPIII (NAALADase2) and NAALADase L. These proteins belong to the peptidase ...
    cd08022
    Location:314 → 555
    M28_PSMA_like; M28 Zn-peptidase prostate-specific membrane antigen
    pfam04253
    Location:587 → 707
    TFR_dimer; Transferrin receptor-like dimerization domain

  2. XM_011241861.4 → XP_011240163.1  glutamate carboxypeptidase 2 isoform X2

    Conserved Domains (2) summary
    cd02121
    Location:122 → 348
    PA_GCPII_like; Protease-associated domain containing protein, glutamate carboxypeptidase II (GCPII)-like. This group contains various PA domain-containing proteins similar to GCPII including, GCPIII (NAALADase2) and NAALADase L. These proteins belong to the peptidase ...
    cl14876
    Location:356 → 438
    Zinc_peptidase_like; Zinc peptidases M18, M20, M28, and M42

RNA

  1. XR_004934143.1 RNA Sequence

  2. XR_003946512.2 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O35409.2 GenPept UniProtKB/Swiss-Prot:O35409

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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