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PHB prohibitin [ Homo sapiens (human) ]

Gene ID: 5245, updated on 5-Jul-2020

Summary

Official Symbol
PHBprovided by HGNC
Official Full Name
prohibitinprovided by HGNC
Primary source
HGNC:HGNC:8912
See related
Ensembl:ENSG00000167085 MIM:176705
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
PHB1; HEL-215; HEL-S-54e
Summary
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Expression
Ubiquitous expression in kidney (RPKM 68.3), colon (RPKM 44.2) and 25 other tissues See more
Orthologs

Genomic context

See PHB in Genome Data Viewer
Location:
17q21.33
Exon count:
8
Annotation release Status Assembly Chr Location
109.20200522 current GRCh38.p13 (GCF_000001405.39) 17 NC_000017.11 (49404048..49414905, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (47481410..47492267, complement)

Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene zinc finger protein 652 Neighboring gene uncharacterized LOC102724596 Neighboring gene ribosomal protein L21 pseudogene 124 Neighboring gene eukaryotic translation initiation factor 4E pseudogene 2 Neighboring gene long intergenic non-protein coding RNA 2075

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phenotypes

NHGRI GWAS Catalog

Description
Genome-wide association study identifies eight loci associated with blood pressure.
NHGRI GWA Catalog
Identification of 23 new prostate cancer susceptibility loci using the iCOGS custom genotyping array.
NHGRI GWA Catalog

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify prohibitin (PHB), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
env HIV-1 gp120 upregulates the expression of prohibitin in umbilical cord blood mononuclear cells and T-cell lines PubMed
Envelope surface glycoprotein gp160, precursor env HIV-1 gp160 is identified to have a physical interaction with prohibitin 1 (PHB1) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
env The C-terminal cytoplasmic domain (Env-CT) of HIV-1 Env specifically binds to the Phb1/Phb2 complex. Strong binding is dependent on Env residues 790-800. L799R/L800Q double mutation in Env severely inhibits its binding to the Phb1/Phb2 complex PubMed
Envelope transmembrane glycoprotein gp41 env HIV-1 gp41 is identified to have a physical interaction with prohibitin (PHB) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify prohibitin (PHB), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Nef nef Tandem affinity purification and mass spectrometry analysis identify prohibitin (PHB), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Pr55(Gag) gag Tandem affinity purification and mass spectrometry analysis identify prohibitin (PHB), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Tat tat Expression of HIV-1 Tat upregulates the abundance of prohibitin (PHB) in the nucleoli of Jurkat T-cells PubMed
Vpr vpr A stable-isotope labeling by amino acids in cell culture coupled with mass spectrometry-based proteomics identifies downregulation of prohibitin (PHB) expression by HIV-1 Vpr in Vpr transduced macrophages PubMed
vpr The proteomic assay from Vpr-expressing HTLV-1 transformed cells reveals apoptosis related protein changes, such as mitochondrial protein prohibitin PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

  • Homologs of the PHB gene: The PHB gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, rice, and frog.
  • Orthologs from Annotation Pipeline: 322 organisms have orthologs with human gene PHB
  • Orthologs

Gene Ontology Provided by GOA

Function Evidence Code Pubs
DNA-binding transcription factor activity, RNA polymerase II-specific IEA
Inferred from Electronic Annotation
more info
 
complement component C3a binding IDA
Inferred from Direct Assay
more info
PubMed 
complement component C3b binding IDA
Inferred from Direct Assay
more info
PubMed 
enzyme binding IPI
Inferred from Physical Interaction
more info
PubMed 
histone deacetylase binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein C-terminus binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
proteinase activated receptor binding IPI
Inferred from Physical Interaction
more info
PubMed 
transcription regulatory region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
Process Evidence Code Pubs
DNA biosynthetic process IEA
Inferred from Electronic Annotation
more info
 
cellular response to interleukin-6 IDA
Inferred from Direct Assay
more info
PubMed 
histone deacetylation IDA
Inferred from Direct Assay
more info
PubMed 
mitochondrial calcium ion transmembrane transport TAS
Traceable Author Statement
more info
 
mitochondrion organization IMP
Inferred from Mutant Phenotype
more info
PubMed 
negative regulation of ERK1 and ERK2 cascade IMP
Inferred from Mutant Phenotype
more info
PubMed 
negative regulation of androgen receptor signaling pathway IDA
Inferred from Direct Assay
more info
PubMed 
negative regulation of androgen receptor signaling pathway IMP
Inferred from Mutant Phenotype
more info
PubMed 
negative regulation of cell growth IMP
Inferred from Mutant Phenotype
more info
PubMed 
negative regulation of cell proliferation IMP
Inferred from Mutant Phenotype
more info
PubMed 
negative regulation of glucocorticoid receptor signaling pathway IDA
Inferred from Direct Assay
more info
PubMed 
negative regulation of protein catabolic process IMP
Inferred from Mutant Phenotype
more info
PubMed 
negative regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
PubMed 
negative regulation of transcription by competitive promoter binding IDA
Inferred from Direct Assay
more info
PubMed 
negative regulation of transcription, DNA-templated IDA
Inferred from Direct Assay
more info
PubMed 
negative regulation of transcription, DNA-templated IMP
Inferred from Mutant Phenotype
more info
PubMed 
osteoblast differentiation HDA PubMed 
positive regulation of ERK1 and ERK2 cascade IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of G protein-coupled receptor signaling pathway IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of cell death IGI
Inferred from Genetic Interaction
more info
PubMed 
positive regulation of complement activation IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of gene expression IGI
Inferred from Genetic Interaction
more info
PubMed 
positive regulation of transcription, DNA-templated IDA
Inferred from Direct Assay
more info
PubMed 
progesterone receptor signaling pathway IDA
Inferred from Direct Assay
more info
PubMed 
protein stabilization IMP
Inferred from Mutant Phenotype
more info
PubMed 
regulation of apoptotic process TAS
Traceable Author Statement
more info
PubMed 
regulation of transcription, DNA-templated IDA
Inferred from Direct Assay
more info
PubMed 
signal transduction TAS
Traceable Author Statement
more info
PubMed 
Component Evidence Code Pubs
cell surface IDA
Inferred from Direct Assay
more info
PubMed 
cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
early endosome IDA
Inferred from Direct Assay
more info
PubMed 
extracellular exosome HDA PubMed 
integral component of plasma membrane IDA
Inferred from Direct Assay
more info
PubMed 
membrane HDA PubMed 
mitochondrial inner membrane IDA
Inferred from Direct Assay
more info
PubMed 
mitochondrial inner membrane TAS
Traceable Author Statement
more info
PubMed 
mitochondrion HDA PubMed 
mitochondrion IDA
Inferred from Direct Assay
more info
PubMed 
nucleoplasm IDA
Inferred from Direct Assay
more info
PubMed 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
nucleus TAS
Traceable Author Statement
more info
PubMed 
plasma membrane IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
prohibitin
Names
epididymis luminal protein 215
epididymis secretory sperm binding protein Li 54e

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_023046.1 RefSeqGene

    Range
    4976..15833
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001281496.1NP_001268425.1  prohibitin isoform 1

    See identical proteins and their annotated locations for NP_001268425.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes isoform 1. Variants 1, 2 and 4 encode the same isoform (1).
    Source sequence(s)
    AA357151, AA709387, BE729762, BP267961, CX784018, DC360133
    Consensus CDS
    CCDS11548.1
    UniProtKB/Swiss-Prot
    P35232
    UniProtKB/TrEMBL
    A8K401
    Related
    ENSP00000484113.1, ENST00000617874.4
    Conserved Domains (1) summary
    cd03401
    Location:27221
    SPFH_prohibitin; Prohibitin family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily
  2. NM_001281497.1NP_001268426.1  prohibitin isoform 2

    See identical proteins and their annotated locations for NP_001268426.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) uses alternate in-frame splice sites and lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1.
    Source sequence(s)
    AA357151, AA709387, AC091180, AK302258, BP267961
    Consensus CDS
    CCDS62244.1
    UniProtKB/Swiss-Prot
    P35232
    Related
    ENSP00000425035.1, ENST00000511832.5
    Conserved Domains (1) summary
    cd03401
    Location:27141
    SPFH_prohibitin; Prohibitin family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily
  3. NM_001281715.1NP_001268644.1  prohibitin isoform 1

    See identical proteins and their annotated locations for NP_001268644.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 4 encode the same isoform (1).
    Source sequence(s)
    AA357151, AA709387, AC091180, BF038604, BG029858, BI871461, BP267961
    Consensus CDS
    CCDS11548.1
    UniProtKB/Swiss-Prot
    P35232
    UniProtKB/TrEMBL
    A8K401
    Related
    ENSP00000479488.1, ENST00000614445.4
    Conserved Domains (1) summary
    cd03401
    Location:27221
    SPFH_prohibitin; Prohibitin family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily
  4. NM_002634.4NP_002625.1  prohibitin isoform 1

    See identical proteins and their annotated locations for NP_002625.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 4 encode the same isoform (1).
    Source sequence(s)
    AC091180
    Consensus CDS
    CCDS11548.1
    UniProtKB/Swiss-Prot
    P35232
    UniProtKB/TrEMBL
    A8K401, Q53FV0
    Related
    ENSP00000300408.3, ENST00000300408.7
    Conserved Domains (1) summary
    cd03401
    Location:27221
    SPFH_prohibitin; Prohibitin family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000017.11 Reference GRCh38.p13 Primary Assembly

    Range
    49404048..49414905 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017024763.1XP_016880252.1  prohibitin isoform X1

    UniProtKB/Swiss-Prot
    P35232
    UniProtKB/TrEMBL
    A8K401
    Conserved Domains (1) summary
    cd03401
    Location:27221
    SPFH_prohibitin; Prohibitin family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily
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