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PF4 platelet factor 4 [ Homo sapiens (human) ]

Gene ID: 5196, updated on 5-Aug-2022

Summary

Official Symbol
PF4provided by HGNC
Official Full Name
platelet factor 4provided by HGNC
Primary source
HGNC:HGNC:8861
See related
Ensembl:ENSG00000163737 MIM:173460; AllianceGenome:HGNC:8861
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
PF-4; CXCL4; SCYB4
Summary
This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. The protein also exhibits antimicrobial activity against Plasmodium falciparum. [provided by RefSeq, Oct 2014]
Expression
Biased expression in bone marrow (RPKM 14.3), spleen (RPKM 2.7) and 1 other tissue See more
Orthologs
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Genomic context

See PF4 in Genome Data Viewer
Location:
4q13.3
Exon count:
3
Annotation release Status Assembly Chr Location
110 current GRCh38.p14 (GCF_000001405.40) 4 NC_000004.12 (73980811..73982124, complement)
110 current T2T-CHM13v2.0 (GCF_009914755.1) 4 NC_060928.1 (77324459..77325772, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 4 NC_000004.11 (74846528..74847841, complement)

Chromosome 4 - NC_000004.12Genomic Context describing neighboring genes Neighboring gene heterogeneous nuclear ribonucleoprotein A1 pseudogene 55 Neighboring gene C-X-C motif chemokine ligand 1 pseudogene 1 Neighboring gene pro-platelet basic protein Neighboring gene uncharacterized LOC124900715 Neighboring gene C-X-C motif chemokine ligand 5

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phenotypes

EBI GWAS Catalog

Description
Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease.
EBI GWAS Catalog
Novel Loci for metabolic networks and multi-tissue expression studies reveal genes for atherosclerosis.
EBI GWAS Catalog

HIV-1 interactions

Replication interactions

Interaction Pubs
In single-round infection assays, HIV-1 NL4-3 psuedotyped with Env proteins from subtypes B and C entry is enhanced by high concentrations of tetrameric PF4 (CXCR4) in MAGI-R5 cells PubMed
HIV-1 CH077 virus entry is enhanced by high concentrations of PF4 (CXCR4) in MAGI-R5, SupT1-R5, Jurkat-R5, and primary CD4+ T cells PubMed
In single-round infection assays, HIV-1 NL4-3 pseudotyped with Env proteins from subtypes B and C entry is enhanced by high concentrations of tetrameric PF4 (CXCR4) in MAGI-R5 cells PubMed
HIV-1 CH077 virus entry is inhibited by low concentrations of PF4 (CXCR4) in MAGI-R5, SupT1-R5, Jurkat-R5, and primary CD4+ T cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 JRFL Env (gp120) binds specifically to polystyrene-immobilized PF4 (CXCL4) and CCL5 (RANTES) as measured through ELISA PubMed
env CXCL4 directly interacts with HIV-1 gp120 and the CXCL4-binding site is located within the gp120 outer domain (residues 350-455) proximal to the CD4-binding site PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • MGC138298

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables CXCR chemokine receptor binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables CXCR3 chemokine receptor binding IDA
Inferred from Direct Assay
more info
PubMed 
enables chemokine activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables chemokine activity TAS
Traceable Author Statement
more info
PubMed 
enables heparin binding IDA
Inferred from Direct Assay
more info
PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
involved_in adenylate cyclase-activating G protein-coupled receptor signaling pathway IDA
Inferred from Direct Assay
more info
PubMed 
involved_in antimicrobial humoral immune response mediated by antimicrobial peptide IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in antimicrobial humoral immune response mediated by antimicrobial peptide IDA
Inferred from Direct Assay
more info
PubMed 
involved_in cellular response to lipopolysaccharide IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in chemokine-mediated signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in cytokine-mediated signaling pathway IDA
Inferred from Direct Assay
more info
PubMed 
involved_in defense response to protozoan IDA
Inferred from Direct Assay
more info
PubMed 
involved_in inflammatory response IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in killing by host of symbiont cells IDA
Inferred from Direct Assay
more info
PubMed 
involved_in leukocyte chemotaxis IDA
Inferred from Direct Assay
more info
PubMed 
involved_in negative regulation of MHC class II biosynthetic process IDA
Inferred from Direct Assay
more info
PubMed 
involved_in negative regulation of angiogenesis IDA
Inferred from Direct Assay
more info
PubMed 
involved_in negative regulation of extrinsic apoptotic signaling pathway in absence of ligand IDA
Inferred from Direct Assay
more info
PubMed 
involved_in negative regulation of megakaryocyte differentiation IDA
Inferred from Direct Assay
more info
PubMed 
involved_in neutrophil chemotaxis IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in platelet activation IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of gene expression IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of macrophage derived foam cell differentiation IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of macrophage differentiation IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of tumor necrosis factor production IDA
Inferred from Direct Assay
more info
PubMed 
involved_in regulation of cell population proliferation IDA
Inferred from Direct Assay
more info
PubMed 
Component Evidence Code Pubs
located_in collagen-containing extracellular matrix HDA PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
located_in extracellular region NAS
Non-traceable Author Statement
more info
PubMed 
located_in extracellular region TAS
Traceable Author Statement
more info
 
is_active_in extracellular space IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
located_in platelet alpha granule lumen TAS
Traceable Author Statement
more info
 

General protein information

Preferred Names
platelet factor 4
Names
C-X-C motif chemokine 4
chemokine (C-X-C motif) ligand 4
iroplact
oncostatin-A

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_032897.1 RefSeqGene

    Range
    5098..6314
    Download
    GenBank, FASTA, Sequence Viewer (Graphics), LRG_636

mRNA and Protein(s)

  1. NM_001363352.1NP_001350281.1  platelet factor 4 isoform 2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site which results in the use of an alternate start codon, compared to variant 1. It encodes a longer protein (isoform 2) with a distinct N-terminus, compared to isoform 1.
    Source sequence(s)
    AC097709
    Conserved Domains (1) summary
    cd00273
    Location:47109
    Chemokine_CXC; Chemokine_CXC: 1 of 4 subgroup designations based on the arrangement of the two N-terminal cysteine residues; includes a number of secreted growth factors and interferons involved in mitogenic, chemotactic, and inflammatory activity; many members ...
  2. NM_002619.4NP_002610.1  platelet factor 4 isoform 1 precursor

    See identical proteins and their annotated locations for NP_002610.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1).
    Source sequence(s)
    AC097709, GD143506, M25897
    Consensus CDS
    CCDS3562.1
    UniProtKB/Swiss-Prot
    P02776, Q9UC65
    Related
    ENSP00000296029.3, ENST00000296029.4
    Conserved Domains (1) summary
    cd00273
    Location:38100
    Chemokine_CXC; Chemokine_CXC: 1 of 4 subgroup designations based on the arrangement of the two N-terminal cysteine residues; includes a number of secreted growth factors and interferons involved in mitogenic, chemotactic, and inflammatory activity; many members ...

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 110 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000004.12 Reference GRCh38.p14 Primary Assembly

    Range
    73980811..73982124 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060928.1 Alternate T2T-CHM13v2.0

    Range
    77324459..77325772 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)