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LARS1 leucyl-tRNA synthetase 1 [ Homo sapiens (human) ]

Gene ID: 51520, updated on 1-Jun-2020

Summary

Official Symbol
LARS1provided by HGNC
Official Full Name
leucyl-tRNA synthetase 1provided by HGNC
Primary source
HGNC:HGNC:6512
See related
Ensembl:ENSG00000133706 MIM:151350
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
LRS; LARS; LEUS; LFIS; ILFS1; LEURS; PIG44; RNTLS; HSPC192; hr025Cl
Summary
This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. A mutation in this gene was found in affected individuals with infantile liver failure syndrome 1. Alternatively spliced transcript variants of this gene have been observed. [provided by RefSeq, Dec 2015]
Expression
Ubiquitous expression in thyroid (RPKM 24.0), brain (RPKM 19.6) and 25 other tissues See more
Orthologs

Genomic context

See LARS1 in Genome Data Viewer
Location:
5q32
Exon count:
32
Annotation release Status Assembly Chr Location
109.20200522 current GRCh38.p13 (GCF_000001405.39) 5 NC_000005.10 (146113026..146182787, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 5 NC_000005.9 (145492589..145562294, complement)

Chromosome 5 - NC_000005.10Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC107986458 Neighboring gene SH3 domain containing ring finger 2 Neighboring gene uncharacterized LOC107986459 Neighboring gene PLAC8 like 1 Neighboring gene ribosomal protein L35a pseudogene 16 Neighboring gene ribosomal protein L35a pseudogene 17 Neighboring gene RNA binding motif protein 27 Neighboring gene RNA, U7 small nuclear 180 pseudogene Neighboring gene POU class 4 homeobox 3

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of leucyl-tRNA synthetase (LARS) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify leucyl-Trna synthetase (LARS), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify leucyl-Trna synthetase (LARS), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Nef nef Tandem affinity purification and mass spectrometry analysis identify leucyl-Trna synthetase (LARS), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Pr55(Gag) gag Tandem affinity purification and mass spectrometry analysis identify leucyl-Trna synthetase (LARS), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
matrix gag HIV-1 MA is identified to have a physical interaction with leucyl-tRNA synthetase (LARS) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • FLJ10595, FLJ21788, KIAA1352

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
GTPase activator activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
aminoacyl-tRNA editing activity IEA
Inferred from Electronic Annotation
more info
 
glutamine-tRNA ligase activity IDA
Inferred from Direct Assay
more info
PubMed 
leucine-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
leucine-tRNA ligase activity TAS
Traceable Author Statement
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
aminoacyl-tRNA metabolism involved in translational fidelity IEA
Inferred from Electronic Annotation
more info
 
cellular response to amino acid starvation IMP
Inferred from Mutant Phenotype
more info
PubMed 
cellular response to amino acid stimulus IMP
Inferred from Mutant Phenotype
more info
PubMed 
cellular response to leucine IMP
Inferred from Mutant Phenotype
more info
PubMed 
cellular response to leucine starvation IMP
Inferred from Mutant Phenotype
more info
PubMed 
glutaminyl-tRNA aminoacylation IDA
Inferred from Direct Assay
more info
PubMed 
leucyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
positive regulation of GTPase activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of TOR signaling IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of TORC1 signaling IMP
Inferred from Mutant Phenotype
more info
PubMed 
regulation of cell size IMP
Inferred from Mutant Phenotype
more info
PubMed 
tRNA aminoacylation for protein translation TAS
Traceable Author Statement
more info
 
Component Evidence Code Pubs
aminoacyl-tRNA synthetase multienzyme complex IDA
Inferred from Direct Assay
more info
PubMed 
cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
cytosol IDA
Inferred from Direct Assay
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
endomembrane system IDA
Inferred from Direct Assay
more info
PubMed 
endoplasmic reticulum IDA
Inferred from Direct Assay
more info
PubMed 
lysosome IMP
Inferred from Mutant Phenotype
more info
PubMed 
nuclear body IDA
Inferred from Direct Assay
more info
 

General protein information

Preferred Names
leucine--tRNA ligase, cytoplasmic
Names
cytoplasmic leucyl-tRNA synthetase
cytosolic leucyl-tRNA synthetase
leucine tRNA ligase 1, cytoplasmic
leucine translase
proliferation-inducing gene 44
NP_001304893.1
NP_001304894.1
NP_057544.2
NP_064502.9
XP_011535958.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_042294.1 RefSeqGene

    Range
    5082..74698
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001317964.1NP_001304893.1  leucine--tRNA ligase, cytoplasmic isoform 3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (3) is shorter than isoform 1.
    Source sequence(s)
    AB037773, AC091887, AK304134, CK902545, DB091795
    UniProtKB/Swiss-Prot
    Q9P2J5
    UniProtKB/TrEMBL
    B4E266
    Related
    ENSP00000501365.1, ENST00000674270.1
    Conserved Domains (5) summary
    cd07959
    Location:709828
    Anticodon_Ia_Leu_AEc; Anticodon-binding domain of archaeal and eukaryotic cytoplasmic leucyl tRNA synthetases
    cd00812
    Location:479711
    LeuRS_core; catalytic core domain of leucyl-tRNA synthetases
    PLN02959
    Location:51016
    PLN02959; aminoacyl-tRNA ligase
    cl00015
    Location:45183
    nt_trans; nucleotidyl transferase superfamily
    cl12020
    Location:810859
    Anticodon_Ia_like; Anticodon-binding domain of class Ia aminoacyl tRNA synthetases and similar domains
  2. NM_001317965.1NP_001304894.1  leucine--tRNA ligase, cytoplasmic isoform 4

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) lacks an alternate exon in the 5' region and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (4) has a shorter N-terminus than isoform 1.
    Source sequence(s)
    AB037773, AC091887, AK295874, CK902545, DB091795
    Consensus CDS
    CCDS83029.1
    UniProtKB/Swiss-Prot
    Q9P2J5
    Related
    ENSP00000426005.1, ENST00000510191.5
    Conserved Domains (5) summary
    cd07959
    Location:701820
    Anticodon_Ia_Leu_AEc; Anticodon-binding domain of archaeal and eukaryotic cytoplasmic leucyl tRNA synthetases
    cd00812
    Location:471703
    LeuRS_core; catalytic core domain of leucyl-tRNA synthetases
    PLN02959
    Location:11008
    PLN02959; aminoacyl-tRNA ligase
    cl00015
    Location:1175
    nt_trans; nucleotidyl transferase superfamily
    cl12020
    Location:802851
    Anticodon_Ia_like; Anticodon-binding domain of class Ia aminoacyl tRNA synthetases and similar domains
  3. NM_016460.3NP_057544.2  leucine--tRNA ligase, cytoplasmic isoform 2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
    Source sequence(s)
    AB037773, AC091887, AK293750, CK902545, DB091795
    UniProtKB/Swiss-Prot
    Q9P2J5
    UniProtKB/TrEMBL
    B4DER1
    Related
    ENSP00000501376.1, ENST00000674447.1
    Conserved Domains (5) summary
    cd07959
    Location:728847
    Anticodon_Ia_Leu_AEc; Anticodon-binding domain of archaeal and eukaryotic cytoplasmic leucyl tRNA synthetases
    cd00812
    Location:498730
    LeuRS_core; catalytic core domain of leucyl-tRNA synthetases
    PLN02959
    Location:51035
    PLN02959; aminoacyl-tRNA ligase
    cl00015
    Location:45202
    nt_trans; nucleotidyl transferase superfamily
    cl12020
    Location:829878
    Anticodon_Ia_like; Anticodon-binding domain of class Ia aminoacyl tRNA synthetases and similar domains
  4. NM_020117.11NP_064502.9  leucine--tRNA ligase, cytoplasmic isoform 1

    See identical proteins and their annotated locations for NP_064502.9

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
    Source sequence(s)
    AB037773, AC091887, CK902545
    Consensus CDS
    CCDS34265.1
    UniProtKB/Swiss-Prot
    Q9P2J5
    Related
    ENSP00000377954.2, ENST00000394434.7
    Conserved Domains (1) summary
    cl26128
    Location:51062
    tRNA-synt_1g; tRNA synthetases class I (M)

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000005.10 Reference GRCh38.p13 Primary Assembly

    Range
    146113026..146182787 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011537656.3XP_011535958.1  leucine--tRNA ligase, cytoplasmic isoform X1

    See identical proteins and their annotated locations for XP_011535958.1

    UniProtKB/Swiss-Prot
    Q9P2J5
    Related
    ENSP00000501434.1, ENST00000674174.1
    Conserved Domains (5) summary
    cd07959
    Location:701820
    Anticodon_Ia_Leu_AEc; Anticodon-binding domain of archaeal and eukaryotic cytoplasmic leucyl tRNA synthetases
    cd00812
    Location:471703
    LeuRS_core; catalytic core domain of leucyl-tRNA synthetases
    PLN02959
    Location:11008
    PLN02959; aminoacyl-tRNA ligase
    cl00015
    Location:1175
    nt_trans; nucleotidyl transferase superfamily
    cl12020
    Location:802851
    Anticodon_Ia_like; Anticodon-binding domain of class Ia aminoacyl tRNA synthetases and similar domains
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