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Chek2 checkpoint kinase 2 [ Mus musculus (house mouse) ]

Gene ID: 50883, updated on 9-Mar-2023

Summary

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Official Symbol
Chek2provided by MGI
Official Full Name
checkpoint kinase 2provided by MGI
Primary source
MGI:MGI:1355321
See related
Ensembl:ENSMUSG00000029521 AllianceGenome:MGI:1355321
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
CHK2; Cds1; Rad53; HUCDS1
Summary
Enables protein kinase activity. Acts upstream of or within cellular response to gamma radiation; peptidyl-serine phosphorylation; and signal transduction by p53 class mediator. Predicted to be located in Golgi apparatus and PML body. Predicted to be active in cytoplasm and nucleus. Predicted to colocalize with chromosome, telomeric region. Is expressed in several structures, including central nervous system; genitourinary system; and liver. Human ortholog(s) of this gene implicated in several diseases, including Li-Fraumeni syndrome 2; breast cancer (multiple); colorectal cancer; osteosarcoma; and prostate cancer. Orthologous to human CHEK2 (checkpoint kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in liver E14 (RPKM 9.9), liver E14.5 (RPKM 8.7) and 23 other tissues See more
Orthologs
human all
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Genomic context

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See Chek2 in Genome Data Viewer
Location:
5; 5 F
Exon count:
18
Annotation release Status Assembly Chr Location
109 current GRCm39 (GCF_000001635.27) 5 NC_000071.7 (110987668..111022006)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 5 NC_000071.6 (110839800..110874140)

Chromosome 5 - NC_000071.7Genomic Context describing neighboring genes Neighboring gene ES cell cis-regulatory motif 10b Neighboring gene unc-51 like kinase 1 Neighboring gene HscB iron-sulfur cluster co-chaperone Neighboring gene tetratricopeptide repeat domain 28 Neighboring gene microRNA 701 Neighboring gene predicted gene, 35628

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. CHK2 is essential for spindle assembly and DNA repair during the first cleavage of mouse embryos. Li XH, et al. Aging (Albany NY), 2020 Jun 2. PMID 32484784, Free PMC Article
  2. Checkpoint kinase Chk2 controls renal Cyp27b1 expression, calcitriol formation, and calcium-phosphate metabolism. Fahkri H, et al. Pflugers Arch, 2015 Sep. PMID 25319519
  3. Chk2 regulates cell cycle progression during mouse oocyte maturation and early embryo development. Dai XX, et al. Mol Cells, 2014 Feb. PMID 24598997, Free PMC Article
  4. Global phosphoproteome profiling reveals unanticipated networks responsive to cisplatin treatment of embryonic stem cells. Pines A, et al. Mol Cell Biol, 2011 Dec. PMID 22006019, Free PMC Article
  5. Mice with the CHEK2*1100delC SNP are predisposed to cancer with a strong gender bias. Bahassi el M, et al. Proc Natl Acad Sci U S A, 2009 Oct 6. PMID 19805189, Free PMC Article

See all (120) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Gluconeogenic enzyme PCK1 deficiency promotes CHK2 O-GlcNAcylation and hepatocellular carcinoma growth upon glucose deprivation.
    Title: Gluconeogenic enzyme PCK1 deficiency promotes CHK2 O-GlcNAcylation and hepatocellular carcinoma growth upon glucose deprivation.
  2. SIRT1 modulates cell cycle progression by regulating CHK2 acetylation-phosphorylation.
    Title: SIRT1 modulates cell cycle progression by regulating CHK2 acetylation-phosphorylation.
  3. FadA promotes DNA damage and progression of Fusobacterium nucleatum-induced colorectal cancer through up-regulation of chk2.
    Title: FadA promotes DNA damage and progression of Fusobacterium nucleatum-induced colorectal cancer through up-regulation of chk2.
  4. CHK2 is essential for spindle assembly and DNA repair during the first cleavage of mouse embryos.
    Title: CHK2 is essential for spindle assembly and DNA repair during the first cleavage of mouse embryos.
  5. this study defines a role for Chk2 in suppressing UV-induced skin carcinogenesis
    Title: Differential Roles of Rad18 and Chk2 in Genome Maintenance and Skin Carcinogenesis Following UV Exposure.
  6. Chk2 deletion in Pthrp KI mice can somewhat rescue defects in osteoblastic bone formation and skeletal growth by enhancing endochondral bone formation and osteogenesis
    Title: DNA damage checkpoint pathway modulates the regulation of skeletal growth and osteoblastic bone formation by parathyroid hormone-related peptide.
  7. work reveals that simulated microgravity promotes the apoptotic response through a combined modulation of the Uev1A/TICAM/TRAF/NF-kappaB-regulated apoptosis and the p53/PCNA- and ATM/ATR-Chk1/2-controlled DNA-damage response pathways.
    Title: Simulated Microgravity Promotes Cell Apoptosis Through Suppressing Uev1A/TICAM/TRAF/NF-κB-Regulated Anti-Apoptosis and p53/PCNA- and ATM/ATR-Chk1/2-Controlled DNA-Damage Response Pathways.
  8. Together, this study described the regulation of Chk2 expression through promoter methylation by Dnmt3b and also presented a novel role of Chk2 during neuronal differentiation, which is independent of its previously known function in DNA damage response.
    Title: RA-Induced Transcriptional Silencing of Checkpoint Kinase-2 through Promoter Methylation by Dnmt3b Is Required for Neuronal Differentiation of P19 Cells.
  9. We conclude that Chk2 regulates renal 25-hydroxyvitamin D 1alpha-hydroxylase expression thereby impacting on calcium and phosphate metabolism.
    Title: Checkpoint kinase Chk2 controls renal Cyp27b1 expression, calcitriol formation, and calcium-phosphate metabolism.
  10. TRIP13-deficient spermatocytes also progress to an H1t-positive stage if ATM activity is attenuated by hypomorphic mutations in Mre11 or Nbs1 or by elimination of the ATM-effector kinase CHK2
    Title: The ATM signaling cascade promotes recombination-dependent pachytene arrest in mouse spermatocytes.
Submit: New GeneRIF Correction See all GeneRIFs (44)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (5)  1 citation
  • Endonuclease-mediated (4) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables protein kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables protein kinase binding ISO
Inferred from Sequence Orthology
more info
  
enables protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
enables protein serine/threonine kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in DNA damage checkpoint signaling ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within DNA damage response ISO
Inferred from Sequence Orthology
more info
 PubMed 
involved_in DNA damage response ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in G2/M transition of mitotic cell cycle ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within apoptotic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cell cycle IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cell division IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cellular response to gamma radiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in double-strand break repair ISO
Inferred from Sequence Orthology
more info
  
involved_in intrinsic apoptotic signaling pathway in response to DNA damage ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitotic DNA damage checkpoint signaling IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
acts_upstream_of_or_within mitotic G2 phase IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitotic intra-S DNA damage checkpoint signaling ISO
Inferred from Sequence Orthology
more info
  
involved_in mitotic spindle assembly ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of DNA damage checkpoint ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within peptidyl-serine phosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in peptidyl-serine phosphorylation ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within phosphorylation IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of DNA-templated transcription ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of anoikis ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of protein phosphorylation ISO
Inferred from Sequence Orthology
more info
  
involved_in protein autophosphorylation ISO
Inferred from Sequence Orthology
more info
  
involved_in protein catabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in protein phosphorylation ISO
Inferred from Sequence Orthology
more info
  
involved_in protein stabilization ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of DNA-templated transcription ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of cell cycle ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of protein catabolic process ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within response to gamma radiation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within signal transduction in response to DNA damage IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within signal transduction in response to DNA damage ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within thymocyte apoptotic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in Golgi apparatus ISO
Inferred from Sequence Orthology
more info
  
located_in PML body ISO
Inferred from Sequence Orthology
more info
  
colocalizes_with chromosome, telomeric region ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
serine/threonine-protein kinase Chk2
Names
CHK2 checkpoint homolog
Cds1 homolog
Rad53 homolog
NP_001350237.1
NP_057890.1
XP_006535131.3
XP_006535132.1
XP_006535133.1
XP_006535134.3
XP_030110519.1
XP_030110520.1
XP_036021156.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001363308.1 → NP_001350237.1  serine/threonine-protein kinase Chk2 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC121934
    Conserved Domains (1) summary
    cd14084
    Location:1 → 251
    STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

  2. NM_016681.4 → NP_057890.1  serine/threonine-protein kinase Chk2 isoform 1

    See identical proteins and their annotated locations for NP_057890.1

    Status: VALIDATED

    Source sequence(s)
    AK044913, BX512104, CJ079787
    Consensus CDS
    CCDS19533.1
    UniProtKB/Swiss-Prot
    Q9Z265
    UniProtKB/TrEMBL
    Q543W6
    Related
    ENSMUSP00000066679.2, ENSMUST00000066160.3
    Conserved Domains (2) summary
    cd00060
    Location:97 → 205
    FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
    cd14084
    Location:217 → 490
    STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000071.7 Reference GRCm39 C57BL/6J

    Range
    110987668..111022006
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006535071.5 → XP_006535134.3  serine/threonine-protein kinase Chk2 isoform X4

    Conserved Domains (2) summary
    cd00060
    Location:157 → 265
    FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
    cl21453
    Location:277 → 484
    PKc_like; Protein Kinases, catalytic domain

  2. XM_006535068.5 → XP_006535131.3  serine/threonine-protein kinase Chk2 isoform X2

    Conserved Domains (2) summary
    cd00060
    Location:157 → 265
    FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
    cd14084
    Location:277 → 550
    STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

  3. XM_030254659.2 → XP_030110519.1  serine/threonine-protein kinase Chk2 isoform X1

    Conserved Domains (2) summary
    cd00060
    Location:157 → 265
    FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
    cd14084
    Location:277 → 550
    STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

  4. XM_006535069.2 → XP_006535132.1  serine/threonine-protein kinase Chk2 isoform X3

    See identical proteins and their annotated locations for XP_006535132.1

    UniProtKB/Swiss-Prot
    Q9Z265
    UniProtKB/TrEMBL
    Q543W6
    Conserved Domains (2) summary
    cd00060
    Location:97 → 205
    FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
    cd14084
    Location:217 → 490
    STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

  5. XM_006535070.2 → XP_006535133.1  serine/threonine-protein kinase Chk2 isoform X3

    See identical proteins and their annotated locations for XP_006535133.1

    UniProtKB/Swiss-Prot
    Q9Z265
    UniProtKB/TrEMBL
    Q543W6
    Conserved Domains (2) summary
    cd00060
    Location:97 → 205
    FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
    cd14084
    Location:217 → 490
    STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

  6. XM_036165263.1 → XP_036021156.1  serine/threonine-protein kinase Chk2 isoform X5

    Conserved Domains (2) summary
    cd00060
    Location:97 → 205
    FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
    cl21453
    Location:217 → 424
    PKc_like; Protein Kinases, catalytic domain

  7. XM_030254660.1 → XP_030110520.1  serine/threonine-protein kinase Chk2 isoform X6

    Conserved Domains (1) summary
    cd14084
    Location:1 → 251
    STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

RNA

  1. XR_004942533.1 RNA Sequence

  2. XR_003955670.2 RNA Sequence

  3. XR_003955671.1 RNA Sequence

  4. XR_001784718.1 RNA Sequence

    Related
    ENSMUST00000199937.5
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9Z265.1 GenPept UniProtKB/Swiss-Prot:Q9Z265

Gene LinkOut

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Chemical Information
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