PNP purine nucleoside phosphorylase [Bos taurus (domestic cattle)] - Gene

Summary

Gene symbol: PNP
Gene description: purine nucleoside phosphorylase
See related: EnsemblRapid:ENSBTAG00070037422
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Bos taurus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos
Also known as: NP
: Try the new Gene page
Try the new Transcripts and proteins table

Genomic context

Location:: chromosome: 10

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_12	current	ARS-UCD2.0 (GCF_002263795.3)	10	NC_037337.1 (26632021..26638316, complement)

Chromosome 10 - NC_037337.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_037337.1 Chromosome 10 Reference ARS-UCD2.0 Primary Assembly

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: Dominette sequence data
BioProject: PRJNA294306
Analysis date: Tue Dec 17 08:57:05 2024

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the biological role of packing of the PNP monomers into a trimeric structure
Title: Purine nucleoside phosphorylase activity decline is linked to the decay of the trimeric form of the enzyme.
The transition-state structure of triple mutant PNP is altered as a consequence of the amino acids in the second sphere from the catalytic site.
Title: Second-sphere amino acids contribute to transition-state structure in bovine purine nucleoside phosphorylase.
PCR amplification of the calf phosphorylase from the calf spleen library, cloning, overexpression of the recombinant PNP, its enzymatic activity and interactions with typical ligands of mammalian wild type PNP are described.
Title: Cloning, expression, purification, and some properties of calf purine nucleoside phosphorylase.
This result questions the involvement of the anionic forms in the reaction catalyzed by trimeric PNPs, and contradicts the hypothesis of a strong hydrogen bond formation between the enzyme Asn 243 residue and the purine N7 position.
Title: Fluorescence studies of calf spleen purine nucleoside phosphorylase (PNP) complexes with guanine and 9-deazaguanine.
crystal structure was refined to a resolution of 2.2 A
Title: Calf spleen purine-nucleoside phosphorylase: crystal structure of the binary complex with a potent multisubstrate analogue inhibitor.
The high-affinity binding of transition-state analogues to NP is not the result of single interactions, but results from a constellation of cooperative hydrogen bond interactions, all of which are required to form the tightly bound complex.
Title: Atomic dissection of the hydrogen bond network for transition-state analogue binding to purine nucleoside phosphorylase.
involvement of the neutral purine molecule, and not its monoanion, as the substrate in the reverse, synthetic reaction
Title: Interactions of calf spleen purine nucleoside phosphorylase with 8-azaguanine, and a bisubstrate analogue inhibitor: implications for the reaction mechanism.
alf spleen enzyme is a homotrimer and previous suggestions for dissociation of this enzyme into more active monomers, upon dilution of the enzyme or addition of phosphate, are incorrect.
Title: Oligomeric structure of mammalian purine nucleoside phosphorylase in solution determined by analytical ultracentrifugation.
PNP purine nucleoside phosphorylase was crystallized in space group P2(1)2(1)2(1) in the presence of (S)-PMPDAP and phosphate, and the resulting structure of the binary PNP/(S)-PMPDAP complex was refined at 2.05A resolution.
Title: Crystal structure of calf spleen purine nucleoside phosphorylase with two full trimers in the asymmetric unit: important implications for the mechanism of catalysis.
crystal structure complexed with analogs of the inhibitor 2-diaminopurine aglycone (purine nucleoside phosphorylase)
Title: Crystal structure of calf spleen purine nucleoside phosphorylase in a complex with multisubstrate analogue inhibitor with 2,6-diaminopurine aglycone.

Submit: New GeneRIF Correction

General gene information

Go to the top of the page Help

Markers

BP230011A10A9 (e-PCR)
- UniSTS:519195

NP (e-PCR), detects polymorphism
- UniSTS:278837

NP (e-PCR), detects polymorphism
- UniSTS:503175

MARC_7197-7198:992008282:1 (e-PCR)
- UniSTS:231308

MARC_7197-7198:996687624:1 (e-PCR)
- UniSTS:231308

Gene Ontology Provided by RefSeq

Function	Evidence Code	Pubs
enables purine-nucleoside phosphorylase activity	IEA Inferred from Electronic Annotation more info	PubMed

Process	Evidence Code	Pubs
involved_in nucleoside metabolic process	IEA Inferred from Electronic Annotation more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: purine nucleoside phosphorylase

Names: inosine phosphorylase; inosine-guanosine phosphorylase; nucleoside phosphorylase

NP_001007819.1

EC 2.4.2.1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001007818.1 → NP_001007819.1 purine nucleoside phosphorylase

See identical proteins and their annotated locations for NP_001007819.1

Status: PROVISIONAL

Source sequence(s)

AF529136

UniProtKB/TrEMBL

A0AAA9TPI6, Q687I9

Related

ENSBTAP00070050476.1, ENSBTAT00070083617.1

Conserved Domains (2) summary

COG0005
Location:20 → 282

XapA; Purine nucleoside phosphorylase [Nucleotide transport and metabolism]

TIGR01700
Location:26 → 280

PNPH; purine nucleoside phosphorylase I, inosine and guanosine-specific