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NONO non-POU domain containing octamer binding [ Homo sapiens (human) ]

Gene ID: 4841, updated on 4-Nov-2018

Summary

Official Symbol
NONOprovided by HGNC
Official Full Name
non-POU domain containing octamer bindingprovided by HGNC
Primary source
HGNC:HGNC:7871
See related
Ensembl:ENSG00000147140 MIM:300084; Vega:OTTHUMG00000021798
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114
Summary
This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]
Expression
Ubiquitous expression in ovary (RPKM 81.7), lymph node (RPKM 64.3) and 25 other tissues See more
Orthologs

Genomic context

See NONO in Genome Data Viewer
Location:
Xq13.1
Exon count:
13
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) X NC_000023.11 (71283192..71301168)
105 previous assembly GRCh37.p13 (GCF_000001405.25) X NC_000023.10 (70503042..70521018)

Chromosome X - NC_000023.11Genomic Context describing neighboring genes Neighboring gene gap junction protein beta 1 Neighboring gene zinc finger MYM-type containing 3 Neighboring gene uncharacterized LOC105373247 Neighboring gene integrin subunit beta 1 binding protein 2 Neighboring gene zinc finger CCHC-type and RNA binding motif containing 1 pseudogene

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Jun 15 11:32:44 2016

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of NONO by shRNA enhances HIV-1 replication in both Jurkat cells and primary CD4+ T-cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Nef nef Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Pr55(Gag) gag Knockdown of NONO expression by shRNA enhances both HIV-1 Gag expression in Jurkat cells and the released p24 levels from these cells PubMed
gag Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Rev rev Co-immunoprecipitation analysis demonstrates that endogenous RBM14 associates with HIV-1 Rev, XPO1, and p54/NRB in cells PubMed
rev HIV-1 Rev interacting protein, non-POU domain containing, octamer-binding (NONO), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells. The interaction of Rev with NONO is increased by RRE PubMed
Vpr vpr A stable-isotope labeling by amino acids in cell culture coupled with mass spectrometry-based proteomics identifies upregulation of non-POU domain containing octamer-binding protein (NONO) expression by HIV-1 Vpr in Vpr transduced macrophages PubMed
capsid gag Knockdown of NONO expression by shRNA enhances both HIV-1 Gag expression in Jurkat cells and the released p24 levels from these cells PubMed
retropepsin gag-pol Positional proteomics analysis identifies the cleavage of human non-POU domain containing, octamer-binding (NONO) at amino acid residues 54-55, 274-275, and 277-278 by the HIV-1 protease PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • Circadian rhythm pathway, organism-specific biosystem (from Pathway Interaction Database)
    Circadian rhythm pathway, organism-specific biosystem
    Circadian rhythm pathway
  • Circadian rythm related genes, organism-specific biosystem (from WikiPathways)
    Circadian rythm related genes, organism-specific biosystemThis is currently not a pathway but a list of circadian rhythm related genes and proteins. The source for this information is the gene ontology. The genes and proteins were filtered for "circadian rh...
  • mRNA Processing, organism-specific biosystem (from WikiPathways)
    mRNA Processing, organism-specific biosystemThis process describes the conversion of precursor messenger RNA into mature messenger RNA (mRNA). The pre-mRNA molecule undergoes three main modifications. These modifications are 5' capping, 3' po...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
DNA-binding transcription factor activity, RNA polymerase II-specific ISA
Inferred from Sequence Alignment
more info
 
E-box binding ISS
Inferred from Sequence or Structural Similarity
more info
 
RNA binding HDA PubMed 
RNA polymerase II distal enhancer sequence-specific DNA binding ISS
Inferred from Sequence or Structural Similarity
more info
 
chromatin binding ISS
Inferred from Sequence or Structural Similarity
more info
 
identical protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
transcription regulatory region sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Process Evidence Code Pubs
DNA recombination IEA
Inferred from Electronic Annotation
more info
 
DNA repair IEA
Inferred from Electronic Annotation
more info
 
RNA splicing IEA
Inferred from Electronic Annotation
more info
 
activation of innate immune response IDA
Inferred from Direct Assay
more info
PubMed 
circadian rhythm ISS
Inferred from Sequence or Structural Similarity
more info
 
innate immune response IEA
Inferred from Electronic Annotation
more info
 
mRNA processing IEA
Inferred from Electronic Annotation
more info
 
negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway IDA
Inferred from Direct Assay
more info
PubMed 
negative regulation of transcription, DNA-templated ISS
Inferred from Sequence or Structural Similarity
more info
 
regulation of circadian rhythm ISS
Inferred from Sequence or Structural Similarity
more info
 
regulation of transcription by RNA polymerase II IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
RNA polymerase II transcription factor complex ISS
Inferred from Sequence or Structural Similarity
more info
 
membrane HDA PubMed 
nuclear matrix IDA
Inferred from Direct Assay
more info
PubMed 
nuclear speck IEA
Inferred from Electronic Annotation
more info
 
nucleolus IEA
Inferred from Electronic Annotation
more info
 
nucleoplasm IDA
Inferred from Direct Assay
more info
 
nucleus IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
nucleus ISS
Inferred from Sequence or Structural Similarity
more info
 
paraspeckles IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
non-POU domain-containing octamer-binding protein
Names
54 kDa nuclear RNA- and DNA-binding protein
55 kDa nuclear protein
DNA-binding p52/p100 complex, 52 kDa subunit
non-POU domain-containing octamer (ATGCAAAT) binding protein
p54(nrb)
protein phosphatase 1, regulatory subunit 114

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_046742.1 RefSeqGene

    Range
    5001..22977
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001145408.1NP_001138880.1  non-POU domain-containing octamer-binding protein isoform 1

    See identical proteins and their annotated locations for NP_001138880.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
    Source sequence(s)
    BC002364, BC003129, DA116840, DA779425
    Consensus CDS
    CCDS14410.1
    UniProtKB/Swiss-Prot
    Q15233
    UniProtKB/TrEMBL
    A0A0S2Z4Z9
    Related
    ENSP00000362963.3, OTTHUMP00000023507, ENST00000373856.7, OTTHUMT00000057139
    Conserved Domains (3) summary
    cd12946
    Location:219311
    NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins
    cd12588
    Location:73143
    RRM1_p54nrb; RNA recognition motif 1 in vertebrate 54 kDa nuclear RNA- and DNA-binding protein (p54nrb)
    cl17169
    Location:149228
    RRM_SF; RNA recognition motif (RRM) superfamily
  2. NM_001145409.1NP_001138881.1  non-POU domain-containing octamer-binding protein isoform 1

    See identical proteins and their annotated locations for NP_001138881.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1).
    Source sequence(s)
    BC002364, BC012141, DA116840, DA779425
    Consensus CDS
    CCDS14410.1
    UniProtKB/Swiss-Prot
    Q15233
    UniProtKB/TrEMBL
    A0A0S2Z4Z9
    Related
    ENSP00000362947.1, OTTHUMP00000023508, ENST00000373841.5, OTTHUMT00000057140
    Conserved Domains (3) summary
    cd12946
    Location:219311
    NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins
    cd12588
    Location:73143
    RRM1_p54nrb; RNA recognition motif 1 in vertebrate 54 kDa nuclear RNA- and DNA-binding protein (p54nrb)
    cl17169
    Location:149228
    RRM_SF; RNA recognition motif (RRM) superfamily
  3. NM_001145410.1NP_001138882.1  non-POU domain-containing octamer-binding protein isoform 2

    See identical proteins and their annotated locations for NP_001138882.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
    Source sequence(s)
    AK297144, BC002364, DA116840, DA779425
    Consensus CDS
    CCDS55445.1
    UniProtKB/Swiss-Prot
    Q15233
    Related
    ENSP00000441364.1, ENST00000535149.5
    Conserved Domains (2) summary
    cd12946
    Location:130222
    NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins
    cl17169
    Location:60139
    RRM_SF; RNA recognition motif (RRM) superfamily
  4. NM_007363.4NP_031389.3  non-POU domain-containing octamer-binding protein isoform 1

    See identical proteins and their annotated locations for NP_031389.3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1).
    Source sequence(s)
    BC002364, BC028299, DA116840, DA779425
    Consensus CDS
    CCDS14410.1
    UniProtKB/Swiss-Prot
    Q15233
    UniProtKB/TrEMBL
    A0A0S2Z4Z9
    Related
    ENSP00000276079.8, OTTHUMP00000023506, ENST00000276079.12, OTTHUMT00000057138
    Conserved Domains (3) summary
    cd12946
    Location:219311
    NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins
    cd12588
    Location:73143
    RRM1_p54nrb; RNA recognition motif 1 in vertebrate 54 kDa nuclear RNA- and DNA-binding protein (p54nrb)
    cl17169
    Location:149228
    RRM_SF; RNA recognition motif (RRM) superfamily

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000023.11 Reference GRCh38.p12 Primary Assembly

    Range
    71283192..71301168
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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