Rhox8 reproductive homeobox 8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rhox8provided by MGI
Official Full Name: reproductive homeobox 8provided by MGI
Primary source: MGI:MGI:3579898
See related: Ensembl:ENSMUSG00000064137 AllianceGenome:MGI:3579898
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Tox
Summary: Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific. Acts upstream of or within regulation of gene expression and spermatogenesis. Located in nucleus. [provided by Alliance of Genome Resources, Jul 2025]
Expression: Restricted expression toward testis adult (RPKM 2.9) See more
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Genomic context

Location:: X A3.3; X 22.12 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	X	NC_000086.8 (36963657..36979643, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	X	NC_000086.7 (37874780..37890766, complement)

Chromosome X - NC_000086.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

male RHOX8-knockdown animals showed significantly reduced spermatogenic output, increased germ cell apoptosis, and compromised sperm motility, leading to impaired fertility.
Title: Rhox8 Ablation in the Sertoli Cells Using a Tissue-Specific RNAi Approach Results in Impaired Male Fertility in Mice.
Tox is regulated by calcineurin/Nfat signalling.
Title: Tox: a multifunctional transcription factor and novel regulator of mammalian corticogenesis.
suggests a model in which Rhox8 transcription is dependent upon RHOX5 during early folliculogenesis and upon progesterone during the periovulatory window when RHOX5 normally wanes.
Title: Regulated expression of Rhox8 in the mouse ovary: evidence for the role of progesterone and RHOX5 in granulosa cells.
TOX-dependent transition to the CD4(+)CD8(lo) stage is required for continued development of class II major histocompatibility complex-specific T cells, regardless of ultimate lineage fate
Title: Development of all CD4 T lineages requires nuclear factor TOX.
Tox transcripts were detected predominately in the testis and ovary of mice
Title: A novel PEPP homeobox gene, TOX, is highly glutamic acid rich and specifically expressed in murine testis and ovary.

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General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: reproductive homeobox on X chromosome, 8

Names: homeoprotein TOX

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001004193.3 → NP_001004193.1 reproductive homeobox on X chromosome, 8

See identical proteins and their annotated locations for NP_001004193.1

Status: VALIDATED

Source sequence(s)

AL590629, AY329370

Consensus CDS

CCDS30082.1

UniProtKB/TrEMBL

Q4TU85, Q6VSS7

Related

ENSMUSP00000071782.6, ENSMUST00000071885.7

Conserved Domains (1) summary

cd00086
Location:261 → 317

homeodomain; Homeodomain; DNA binding domains involved in the transcriptional regulation of key eukaryotic developmental processes; may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner.

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000086.8 Reference GRCm39 C57BL/6J

Range

36963657..36979643 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011251014.1 → XP_011249316.1 reproductive homeobox on X chromosome, 8 isoform X1

See identical proteins and their annotated locations for XP_011249316.1

UniProtKB/TrEMBL

Q4TU85

Conserved Domains (1) summary

cd00086
Location:237 → 293

homeodomain; Homeodomain; DNA binding domains involved in the transcriptional regulation of key eukaryotic developmental processes; may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner.