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MCM3 minichromosome maintenance complex component 3 [ Homo sapiens (human) ]

Gene ID: 4172, updated on 9-Dec-2018

Summary

Official Symbol
MCM3provided by HGNC
Official Full Name
minichromosome maintenance complex component 3provided by HGNC
Primary source
HGNC:HGNC:6945
See related
Ensembl:ENSG00000112118 MIM:602693
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
HCC5; P1.h; RLFB; P1-MCM3
Summary
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018]
Expression
Ubiquitous expression in lymph node (RPKM 33.0), appendix (RPKM 23.5) and 24 other tissues See more
Orthologs

Genomic context

See MCM3 in Genome Data Viewer
Location:
6p12.2
Exon count:
17
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 6 NC_000006.12 (52264014..52284881, complement)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 6 NC_000006.11 (52128812..52149679, complement)

Chromosome 6 - NC_000006.12Genomic Context describing neighboring genes Neighboring gene solute carrier family 25 member 20 pseudogene 1 Neighboring gene interleukin 17F Neighboring gene progestin and adipoQ receptor family member 8 Neighboring gene VISTA enhancer hs2064 Neighboring gene EF-hand domain containing 1

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Jun 15 11:32:44 2016

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Rev rev HIV-1 Rev co-localizes with minichromosome maintenance complex component 3 (MCM3) in the cytoplasmic and nucleoplasmic compartments of cells PubMed
Tat tat HIV-1 Tat upregulates the expression of minichromosome maintenance complex component 3 (MCM3) in Jurkat cells PubMed
tat Interaction of HIV-1 Tat with MCM3 in T-cells is identified by a proteomic strategy based on affinity chromatography coupled with mass spectrometry PubMed
Vpr vpr HIV-1 Vpr downregulates MCM3 in HeLa cells within 12 hours of exposure PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • Activation of ATR in response to replication stress, organism-specific biosystem (from REACTOME)
    Activation of ATR in response to replication stress, organism-specific biosystemGenotoxic stress caused by DNA damage or stalled replication forks can lead to genomic instability. To guard against such instability, genotoxically-stressed cells activate checkpoint factors that ha...
  • Activation of the pre-replicative complex, organism-specific biosystem (from REACTOME)
    Activation of the pre-replicative complex, organism-specific biosystemIn S. cerevisiae, two ORC subunits, Orc1 and Orc5, both bind ATP, and Orc1 in addition has ATPase activity. Both ATP binding and ATP hydrolysis appear to be essential functions in vivo. ATP binding b...
  • Assembly of the pre-replicative complex, organism-specific biosystem (from REACTOME)
    Assembly of the pre-replicative complex, organism-specific biosystemDNA replication pre-initiation in eukaryotic cells begins with the formation of the pre-replicative complex (pre-RC) during the late M phase and continues in the G1 phase of the mitotic cell cycle, a...
  • Cell Cycle, organism-specific biosystem (from WikiPathways)
    Cell Cycle, organism-specific biosystemThe cell cycle is the series of events that takes place in a cell leading to its division and duplication (replication). Regulation of the cell cycle involves processes crucial to the survival of a c...
  • Cell Cycle, organism-specific biosystem (from REACTOME)
    Cell Cycle, organism-specific biosystem
    Cell Cycle
  • Cell Cycle Checkpoints, organism-specific biosystem (from REACTOME)
    Cell Cycle Checkpoints, organism-specific biosystemA hallmark of the human cell cycle in normal somatic cells is its precision. This remarkable fidelity is achieved by a number of signal transduction pathways, known as checkpoints, which monitor cell...
  • Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
    Cell Cycle, Mitotic, organism-specific biosystemThe replication of the genome and the subsequent segregation of chromosomes into daughter cells are controlled by a series of events collectively known as the cell cycle. DNA replication is carried o...
  • Cell cycle, organism-specific biosystem (from KEGG)
    Cell cycle, organism-specific biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cycli...
  • Cell cycle, conserved biosystem (from KEGG)
    Cell cycle, conserved biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cycli...
  • DNA Replication, organism-specific biosystem (from REACTOME)
    DNA Replication, organism-specific biosystemStudies in the past decade have suggested that the basic mechanism of DNA replication initiation is conserved in all kingdoms of life. Initiation in unicellular eukaryotes, in particular Saccharomyce...
  • DNA Replication, organism-specific biosystem (from WikiPathways)
    DNA Replication, organism-specific biosystemStudies in the past decade have suggested that the basic mechanism of DNA replication initiation is conserved in all kingdoms of life. Initiation in unicellular eukaryotes, in particular Saccharomyce...
  • DNA Replication Pre-Initiation, organism-specific biosystem (from REACTOME)
    DNA Replication Pre-Initiation, organism-specific biosystemAlthough, DNA replication occurs in the S phase of the cell cycle, the formation of the DNA replication pre-initiation complex begins during G1 phase.
  • DNA replication, organism-specific biosystem (from KEGG)
    DNA replication, organism-specific biosystemA complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (D...
  • DNA replication, conserved biosystem (from KEGG)
    DNA replication, conserved biosystemA complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (D...
  • DNA strand elongation, organism-specific biosystem (from REACTOME)
    DNA strand elongation, organism-specific biosystemAccurate and efficient genome duplication requires coordinated processes to replicate two template strands at eucaryotic replication forks. Knowledge of the fundamental reactions involved in replicat...
  • E2F transcription factor network, organism-specific biosystem (from Pathway Interaction Database)
    E2F transcription factor network, organism-specific biosystem
    E2F transcription factor network
  • G1 to S cell cycle control, organism-specific biosystem (from WikiPathways)
    G1 to S cell cycle control, organism-specific biosystemIn the G1 phase there are two types of DNA damage responses, the p53-dependent and the p53-independent pathways. The p53-dependent responses inhibit CDKs through the up-regulation of genes encoding C...
  • G1/S Transition, organism-specific biosystem (from REACTOME)
    G1/S Transition, organism-specific biosystemCyclin E - Cdk2 complexes control the transition from G1 into S-phase. In this case, the binding of p21Cip1/Waf1 or p27kip1 is inhibitory. Important substrates for Cyclin E - Cdk2 complexes include p...
  • G2/M Checkpoints, organism-specific biosystem (from REACTOME)
    G2/M Checkpoints, organism-specific biosystemG2/M checkpoints include the checks for damaged DNA, unreplicated DNA, and checks that ensure that the genome is replicated once and only once per cell cycle. If cells pass these checkpoints, they f...
  • M/G1 Transition, organism-specific biosystem (from REACTOME)
    M/G1 Transition, organism-specific biosystemFinally, progression out of mitosis and division of the cell into two daughters (cytokinesis) requires the inactivation of Cyclin B - Cdc2 by ubiquitin-dependent proteolysis of Cyclin A and B, which ...
  • MCM complex, organism-specific biosystem (from KEGG)
    MCM complex, organism-specific biosystemStructural complex; Genetic information processing; Replication system
  • MCM complex, conserved biosystem (from KEGG)
    MCM complex, conserved biosystemStructural complex; Genetic information processing; Replication system
  • Mitotic G1-G1/S phases, organism-specific biosystem (from REACTOME)
    Mitotic G1-G1/S phases, organism-specific biosystem
    Mitotic G1-G1/S phases
  • Orc1 removal from chromatin, organism-specific biosystem (from REACTOME)
    Orc1 removal from chromatin, organism-specific biosystem
    Orc1 removal from chromatin
  • Regulation of DNA replication, organism-specific biosystem (from REACTOME)
    Regulation of DNA replication, organism-specific biosystemDNA replication is regulated at various levels via ORC proteins. This pathway includes annotation of individual events that lead to the regulation of replication.
  • Removal of licensing factors from origins, organism-specific biosystem (from REACTOME)
    Removal of licensing factors from origins, organism-specific biosystemLicensing factors are removed from the origin by various means like biochemical modification (phosphorylation) or by physical association with other proteins. This pathway includes the annotations of...
  • Retinoblastoma (RB) in Cancer, organism-specific biosystem (from WikiPathways)
    Retinoblastoma (RB) in Cancer, organism-specific biosystemDescribes the role of retinoblastoma (RB) gene in cancer.
  • S Phase, organism-specific biosystem (from REACTOME)
    S Phase, organism-specific biosystemDNA synthesis occurs in the S phase, or the synthesis phase, of the cell cycle. The cell duplicates its hereditary material, and two copies of the chromosome are formed. As DNA replication continues,...
  • Switching of origins to a post-replicative state, organism-specific biosystem (from REACTOME)
    Switching of origins to a post-replicative state, organism-specific biosystem
    Switching of origins to a post-replicative state
  • Synthesis of DNA, organism-specific biosystem (from REACTOME)
    Synthesis of DNA, organism-specific biosystemThe actual synthesis of DNA occurs in the S phase of the cell cycle. This includes the initiation of DNA replication, when the first nucleotide of the new strand is laid down during the synthesis of ...
  • Unwinding of DNA, organism-specific biosystem (from REACTOME)
    Unwinding of DNA, organism-specific biosystemDNA Replication is regulated accurately and precisely by various protein complexes. Many members of the MCM protein family are assembled into the pre-Replication Complexes (pre-RC) at the end of M ph...

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • MGC1157

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
DNA binding IEA
Inferred from Electronic Annotation
more info
 
helicase activity IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
DNA replication TAS
Traceable Author Statement
more info
 
DNA replication initiation IEA
Inferred from Electronic Annotation
more info
 
G1/S transition of mitotic cell cycle TAS
Traceable Author Statement
more info
 
Component Evidence Code Pubs
MCM complex IDA
Inferred from Direct Assay
more info
PubMed 
alpha DNA polymerase:primase complex TAS
Traceable Author Statement
more info
PubMed 
centrosome IDA
Inferred from Direct Assay
more info
 
membrane HDA PubMed 
nuclear chromosome, telomeric region HDA PubMed 
NOT nucleolus IDA
Inferred from Direct Assay
more info
PubMed 
nucleoplasm IDA
Inferred from Direct Assay
more info
 
nucleoplasm TAS
Traceable Author Statement
more info
 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
perinuclear region of cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
DNA replication licensing factor MCM3
Names
DNA polymerase alpha holoenzyme-associated protein P1
DNA replication factor MCM3
MCM3 minichromosome maintenance deficient 3
RLF subunit beta
cervical cancer proto-oncogene 5
hRlf beta subunit
minichromosome maintenance deficient 3
p102
replication licensing factor, beta subunit

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001270472.2NP_001257401.2  DNA replication licensing factor MCM3 isoform 2

    Status: REVIEWED

    Source sequence(s)
    AL034343
    Consensus CDS
    CCDS75468.1
    Related
    ENSP00000388647.3, ENST00000419835.7
    Conserved Domains (1) summary
    smart00350
    Location:63608
    MCM; minichromosome maintenance proteins
  2. NM_001366369.1NP_001353298.1  DNA replication licensing factor MCM3 isoform 3

    Status: REVIEWED

    Source sequence(s)
    AL034343
    Conserved Domains (2) summary
    smart00350
    Location:109654
    MCM; minichromosome maintenance proteins
    pfam14551
    Location:19104
    MCM_N; MCM N-terminal domain
  3. NM_001366370.1NP_001353299.1  DNA replication licensing factor MCM3 isoform 4

    Status: REVIEWED

    Source sequence(s)
    AL034343
    Conserved Domains (2) summary
    smart00350
    Location:109671
    MCM; minichromosome maintenance proteins
    pfam14551
    Location:19104
    MCM_N; MCM N-terminal domain
  4. NM_001366371.1NP_001353300.1  DNA replication licensing factor MCM3 isoform 5

    Status: REVIEWED

    Source sequence(s)
    AL034343
    Conserved Domains (2) summary
    smart00350
    Location:109637
    MCM; minichromosome maintenance proteins
    pfam14551
    Location:19104
    MCM_N; MCM N-terminal domain
  5. NM_001366372.1NP_001353301.1  DNA replication licensing factor MCM3 isoform 6

    Status: REVIEWED

    Source sequence(s)
    AL034343
    Conserved Domains (2) summary
    smart00350
    Location:109637
    MCM; minichromosome maintenance proteins
    pfam14551
    Location:19104
    MCM_N; MCM N-terminal domain
  6. NM_001366373.1NP_001353302.1  DNA replication licensing factor MCM3 isoform 7

    Status: REVIEWED

    Source sequence(s)
    AL034343
    Conserved Domains (1) summary
    cl25443
    Location:6481
    MCM_OB; MCM OB domain
  7. NM_001366374.1NP_001353303.1  DNA replication licensing factor MCM3 isoform 8

    Status: REVIEWED

    Source sequence(s)
    AL034343
    Conserved Domains (1) summary
    cl25443
    Location:1451
    MCM_OB; MCM OB domain
  8. NM_001366375.1NP_001353304.1  DNA replication licensing factor MCM3 isoform 9

    Status: REVIEWED

    Source sequence(s)
    AL034343
    Conserved Domains (2) summary
    COG1241
    Location:17165
    Mcm2; DNA replicative helicase MCM subunit Mcm2, Cdc46/Mcm family [Replication, recombination and repair]
    cl25443
    Location:177373
    MCM_OB; MCM OB domain
  9. NM_002388.6NP_002379.4  DNA replication licensing factor MCM3 isoform 1

    Status: REVIEWED

    Source sequence(s)
    AL034343
    Consensus CDS
    CCDS4940.2
    Related
    ENSP00000480987.1, ENST00000616552.4
    Conserved Domains (2) summary
    smart00350
    Location:109654
    MCM; minichromosome maintenance proteins
    pfam14551
    Location:19104
    MCM_N; MCM N-terminal domain

RNA

  1. NR_158963.1 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AL034343
  2. NR_158964.1 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AL034343
  3. NR_158965.1 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AL034343
  4. NR_158966.1 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AL034343

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000006.12 Reference GRCh38.p12 Primary Assembly

    Range
    52264014..52284881 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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