U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

araf A-Raf proto-oncogene, serine/threonine kinase [ Danio rerio (zebrafish) ]

Gene ID: 403063, updated on 9-Sep-2025
Official Symbol
arafprovided by ZNC
Official Full Name
A-Raf proto-oncogene, serine/threonine kinaseprovided by ZNC
Primary source
ZFIN:ZDB-GENE-050102-2
See related
Ensembl:ENSDARG00000054533 AllianceGenome:ZFIN:ZDB-GENE-050102-2
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Danio rerio
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as
sb:eu994; zgc:92074; wu:fk45h07
Summary
Enables SMAD binding activity. Acts upstream of or within negative regulation of SMAD protein signal transduction and negative regulation of nodal signaling pathway. Predicted to be active in cytosol and mitochondrion. Is expressed in head. Human ortholog(s) of this gene implicated in high grade glioma. Orthologous to human ARAF (A-Raf proto-oncogene, serine/threonine kinase). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
NEW
Try the new Gene table
Try the new Transcript table
See araf in Genome Data Viewer
Location:
chromosome: 5
Exon count:
22
Annotation release Status Assembly Chr Location
RS_2025_04 current GRCz12tu (GCF_049306965.1) 5 NC_133180.1 (27465243..27523137)
RS_2024_08 previous assembly GRCz11 (GCF_000002035.6) 5 NC_007116.7 (26853096..26866045)

Chromosome 5 - NC_133180.1Genomic Context describing neighboring genes Neighboring gene si:ch211-102c2.4 Neighboring gene IMP U3 small nucleolar ribonucleoprotein 4 Neighboring gene zgc:64051 Neighboring gene lectin, mannose-binding 2-like b Neighboring gene T-box transcription factor 3b Neighboring gene TM2 domain containing 2

Go to nucleotide: Graphics FASTA GenBank

  • Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
  • Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
  • BioProject: PRJEB1986
  • Analysis date: Fri Dec 8 19:48:10 2017

Related articles in PubMed

  1. The Kinase Activity-deficient Isoform of the Protein Araf Antagonizes Ras/Mitogen-activated Protein Kinase (Ras/MAPK) Signaling in the Zebrafish Embryo. Xiong C, et al. J Biol Chem, 2015 Oct 16. PMID 26306042, Free PMC Article
  2. miR-103/107 regulates left-right asymmetry in zebrafish by modulating Kupffer's vesicle development and ciliogenesis. Heigwer J, et al. Biochem Biophys Res Commun, 2020 Jun 25. PMID 32334837
  3. Araf kinase antagonizes Nodal-Smad2 activity in mesendoderm development by directly phosphorylating the Smad2 linker region. Liu X, et al. Nat Commun, 2013. PMID 23591895, Free PMC Article
  4. Temporal pattern of loss/persistence of duplicate genes involved in signal transduction and metabolic pathways after teleost-specific genome duplication. Sato Y, et al. BMC Evol Biol, 2009 Jun 5. PMID 19500364, Free PMC Article
  5. RFX transcription factors are essential for hearing in mice. Elkon R, et al. Nat Commun, 2015 Oct 15. PMID 26469318, Free PMC Article

See all (8) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. different isoforms of Araf may participate in similar developmental processes but by regulating different signaling pathways
    Title: The Kinase Activity-deficient Isoform of the Protein Araf Antagonizes Ras/Mitogen-activated Protein Kinase (Ras/MAPK) Signaling in the Zebrafish Embryo.
Submit: New GeneRIF Correction

Markers

Homology

Gene Ontology Provided by ZFIN

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables MAP kinase kinase kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables SMAD binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine/threonine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in MAPK cascade IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within negative regulation of SMAD protein signal transduction IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within negative regulation of nodal signaling pathway IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within negative regulation of nodal signaling pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within phosphorylation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within protein phosphorylation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within signal transduction IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
Preferred Names
serine/threonine-protein kinase A-Raf
Names
serine/threonine protein kinase ARAF alt1
serine/threonine protein kinase ARAF alt2
serine/threonine protein kinase ARAF alt3
serine/threonine protein kinase ARAF alt4
serine/threonine protein kinase ARAF alt5
serine/threonine protein kinase ARAF standard form
v-raf murine sarcoma 3611 viral oncogene homolog
NP_991306.1
XP_005171874.1
XP_005171875.1

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_205743.1 → NP_991306.1  serine/threonine-protein kinase A-Raf

    See identical proteins and their annotated locations for NP_991306.1

    Status: PROVISIONAL

    Source sequence(s)
    AB120005
    UniProtKB/TrEMBL
    Q75Y03
    Related
    ENSDARP00000118602.1, ENSDART00000138182.3
    Conserved Domains (4) summary
    cd01816
    Location:24 → 95
    Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases
    cd00029
    Location:103 → 148
    C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
    cd14150
    Location:311 → 575
    STKc_A-Raf; Catalytic domain of the Serine/Threonine Kinase, A-Raf (Rapidly Accelerated Fibrosarcoma) kinase
    pfam00069
    Location:315 → 566
    Pkinase; Protein kinase domain

RefSeqs of Annotated Genomes: GCF_049306965.1-RS_2025_04

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCz12tu Primary Assembly

Genomic

  1. NC_133180.1 Reference GRCz12tu Primary Assembly

    Range
    27465243..27523137
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_005171817.6 → XP_005171874.1  serine/threonine-protein kinase A-Raf isoform X1

    See identical proteins and their annotated locations for XP_005171874.1

    UniProtKB/TrEMBL
    A0A8M2BJ35, Q5FBD1
    Conserved Domains (4) summary
    cd01816
    Location:24 → 95
    Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases
    cd00029
    Location:103 → 148
    C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
    cd14150
    Location:311 → 575
    STKc_A-Raf; Catalytic domain of the Serine/Threonine Kinase, A-Raf (Rapidly Accelerated Fibrosarcoma) kinase
    pfam00069
    Location:315 → 566
    Pkinase; Protein kinase domain

  2. XM_005171818.6 → XP_005171875.1  serine/threonine-protein kinase A-Raf isoform X2

    See identical proteins and their annotated locations for XP_005171875.1

    UniProtKB/TrEMBL
    A0A8M2BJL6, Q5M7Y2
    Related
    ENSDARP00000118701.1
    Conserved Domains (4) summary
    cd01816
    Location:24 → 95
    Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases
    cd00029
    Location:103 → 148
    C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
    cd14150
    Location:307 → 571
    STKc_A-Raf; Catalytic domain of the Serine/Threonine Kinase, A-Raf (Rapidly Accelerated Fibrosarcoma) kinase
    pfam00069
    Location:311 → 562
    Pkinase; Protein kinase domain

RNA

  1. XR_012405825.1 RNA Sequence

  2. XR_012405827.1 RNA Sequence

  3. XR_012405824.1 RNA Sequence

  4. XR_012405826.1 RNA Sequence

  5. XR_012405810.1 RNA Sequence

  6. XR_012405811.1 RNA Sequence

  7. XR_012405812.1 RNA Sequence

  8. XR_012405813.1 RNA Sequence

  9. XR_012405814.1 RNA Sequence

  10. XR_012405815.1 RNA Sequence

  11. XR_012405816.1 RNA Sequence

  12. XR_012405817.1 RNA Sequence

  13. XR_012405818.1 RNA Sequence

  14. XR_012405819.1 RNA Sequence

  15. XR_012405820.1 RNA Sequence

  16. XR_012405821.1 RNA Sequence

  17. XR_012405822.1 RNA Sequence

  18. XR_012405823.1 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.