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Hsp22 Heat shock protein 22 [ Drosophila melanogaster (fruit fly) ]

Gene ID: 3772576, updated on 6-Jul-2026
Official Symbol
Hsp22provided by FlyBase
Official Full Name
Heat shock protein 22provided by FlyBase
Primary source
FLYBASE:FBgn0001223
Locus tag
Dmel_CG4460
See related
AllianceGenome:FB:FBgn0001223
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
22; anon-WO0118547.50; anon-WO0140519.138; CG32041; CG4460; dHsp22; dHSP22; Dmel20.8; Dmel\CG4460; DmHsp22; EP(3)3247; hsp22; HSP22; small hsp locus 67B
Summary
Enables identical protein binding activity and unfolded protein binding activity. Involved in several processes, including chaperone-mediated protein folding; determination of adult lifespan; and response to heat. Located in mitochondrial matrix. Is expressed in ectoderm. Human ortholog(s) of this gene implicated in cataract 16 multiple types; cataract 9 multiple types; dilated cardiomyopathy 1II; fatal infantile hypertonic myofibrillar myopathy; and myofibrillar myopathy 2. Orthologous to human CRYAA (crystallin alpha A); CRYAB (crystallin alpha B); and HSPB2 (heat shock protein family B (small) member 2). [provided by Alliance of Genome Resources, Apr 2025]
Orthologs
all
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See Hsp22 in Genome Data Viewer
Location:
67B2-67B2; 3-29 cM
Exon count:
3
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3L NT_037436.4 (9372931..9374964)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3L NT_037436.3 (9366031..9368064)

Chromosome 3L - NT_037436.4Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene Ferredoxin 1 Neighboring gene Heat shock gene 67Bc Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene Heat shock protein 26

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Association of an S1 nuclease-sensitive structure with short direct repeats 5' of Drosophila heat shock genes. Mace HA, et al. Nature, 1983 Aug 11-17. PMID 6308469
  2. Structural and functional properties of proteins interacting with small heat shock proteins. Dabbaghizadeh A, et al. Cell Stress Chaperones, 2020 Jul. PMID 32314314, Free PMC Article
  3. Oligomeric structure and chaperone-like activity of Drosophila melanogaster mitochondrial small heat shock protein Hsp22 and arginine mutants in the alpha-crystallin domain. Dabbaghizadeh A, et al. Cell Stress Chaperones, 2017 Jul. PMID 28389817, Free PMC Article
  4. The impact of green tea polyphenols on development and reproduction in Drosophila melanogaster. Lopez TE, et al. J Funct Foods, 2016 Jan 1. PMID 26693252, Free PMC Article
  5. Regulatory elements near the Drosophila hsp 22 gene required for ecdysterone and heat shock induction. Rudolph K, et al. Dev Genet, 1991. PMID 1868625

See all (258) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. 60 common DmHsp22-binding mitochondrial partners were detected via immunoaffinity conjugation with mass spectroscopy analysis of mitochondria from HeLa cells transfected with DmHsp22 in non-heat shock condition and after heat shock. Among the partners of DmHsp22, several ATP synthase subunits were found.
    Title: Identification of proteins interacting with the mitochondrial small heat shock protein Hsp22 of Drosophila melanogaster: Implication in mitochondrial homeostasis.
  2. This study shows that mutations of arginine to glycine in Hsp22 alpha-crystallin domain induce a number of structural changes, some of which differ from those described in mammalian small heat shock proteins.
    Title: Oligomeric structure and chaperone-like activity of Drosophila melanogaster mitochondrial small heat shock protein Hsp22 and arginine mutants in the alpha-crystallin domain.
  3. Together the results are consistent with a role of Hsp22 in the UPR(MT) and in mitochondrial proteostasis.
    Title: Changes in Drosophila mitochondrial proteins following chaperone-mediated lifespan extension confirm a role of Hsp22 in mitochondrial UPR and reveal a mitochondrial localization for cathepsin D.
  4. hsp22 could play an important role in relation to stress resistance and adaptation.
    Title: Downregulation of hsp22 gene expression in Drosophila melanogaster from sites located near chemical plants.
  5. Hsp22 and Hsp23 play important roles in the recovery from chill coma in adult males, and suggest that these contribute to adaptive responses to fluctuating thermal conditions.
    Title: Knocking down expression of Hsp22 and Hsp23 by RNA interference affects recovery from chill coma in Drosophila melanogaster.
  6. The up-regulation of genes of the OXPHOS system in Hsp22+ flies suggest that mitochondrial homeostasis is at the center of Hsp22 beneficial effects on lifespan.
    Title: Gene expression profiling implicates OXPHOS complexes in lifespan extension of flies over-expressing a small mitochondrial chaperone, Hsp22.
  7. Data show that the DmHsp22 interacts and inactivates wild type tumor suppressor protein p53, which may be one possible way of its functioning in human cells.
    Title: Proproliferative functions of Drosophila small mitochondrial heat shock protein 22 in human cells.
  8. Life span alteration after irradiation in Drosophila melanogaster strains with mutations of Hsf and Hsps.
    Title: Life span alteration after irradiation in Drosophila melanogaster strains with mutations of Hsf and Hsps.
  9. Expression of hsp22 and hsp70 transgenes is partially predictive of drosophila survival under normal and stress conditions.
    Title: Expression of hsp22 and hsp70 transgenes is partially predictive of drosophila survival under normal and stress conditions.
  10. Drosophila that are not expressing this mitochondrial small Hsp22 have a 40% decrease in lifespan.
    Title: Decreased lifespan in the absence of expression of the mitochondrial small heat shock protein Hsp22 in Drosophila.
Submit: New GeneRIF Correction See all GeneRIFs (11)
Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables unfolded protein holdase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables unfolded protein holdase activity ISM
Inferred from Sequence Model
more info
 PubMed 
Process Evidence Code Pubs
involved_in determination of adult lifespan IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein refolding IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein refolding IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein refolding ISM
Inferred from Sequence Model
more info
 PubMed 
involved_in response to heat IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in response to heat IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in response to heat IEA
Inferred from Electronic Annotation
more info
  
involved_in response to heat IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to oxidative stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in mitochondrial matrix IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
Preferred Names
heat shock protein 22
Names
CG4460-PA
CG4460-PB
Hsp22-PA
Hsp22-PB
heat shock protein hsp22
heat-shock protein 22
protein 22

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_037436.4 Reference assembly

    Range
    9372931..9374964
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001031944.2 → NP_001027115.1  heat shock protein 22, isoform A

    See identical proteins and their annotated locations for NP_001027115.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    A4V1Q5, P02515, Q95SZ4, Q9VSX1
    Conserved Domains (2) summary
    cd06526
    Location:61 → 138
    metazoan_ACD; Alpha-crystallin domain (ACD) of metazoan alpha-crystallin-type small(s) heat shock proteins (Hsps). sHsps are small stress induced proteins with monomeric masses between 12 -43 kDa, whose common feature is the Alpha-crystallin domain (ACD). sHsps are ...
    COG0071
    Location:17 → 154
    IbpA; Molecular chaperone IbpA, HSP20 family [Posttranslational modification, protein turnover, chaperones]

  2. NM_001031943.3 → NP_001027114.1  heat shock protein 22, isoform B

    See identical proteins and their annotated locations for NP_001027114.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    A4V1Q5, P02515, Q95SZ4, Q9VSX1
    Conserved Domains (2) summary
    cd06526
    Location:61 → 138
    metazoan_ACD; Alpha-crystallin domain (ACD) of metazoan alpha-crystallin-type small(s) heat shock proteins (Hsps). sHsps are small stress induced proteins with monomeric masses between 12 -43 kDa, whose common feature is the Alpha-crystallin domain (ACD). sHsps are ...
    COG0071
    Location:17 → 154
    IbpA; Molecular chaperone IbpA, HSP20 family [Posttranslational modification, protein turnover, chaperones]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P02515.4 P02515

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