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ARAF A-Raf proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]

Gene ID: 369, updated on 27-Nov-2024

Summary

Official Symbol
ARAFprovided by HGNC
Official Full Name
A-Raf proto-oncogene, serine/threonine kinaseprovided by HGNC
Primary source
HGNC:HGNC:646
See related
Ensembl:ENSG00000078061 MIM:311010; AllianceGenome:HGNC:646
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
PKS2; A-RAF; ARAF1; RAFA1
Summary
Enables protein serine/threonine kinase activity. Involved in negative regulation of apoptotic process; regulation of TOR signaling; and regulation of protein metabolic process. Predicted to be active in cytosol and mitochondrion. Biomarker of high grade glioma. [provided by Alliance of Genome Resources, Nov 2024]
Expression
Ubiquitous expression in bone marrow (RPKM 16.5), spleen (RPKM 14.0) and 25 other tissues See more
Orthologs
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Genomic context

See ARAF in Genome Data Viewer
Location:
Xp11.3
Exon count:
16
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) X NC_000023.11 (47561205..47571908)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) X NC_060947.1 (46971095..46981798)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) X NC_000023.10 (47420604..47431307)

Chromosome X - NC_000023.11Genomic Context describing neighboring genes Neighboring gene zinc finger protein 41 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29593 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20804 Neighboring gene long intergenic non-protein coding RNA 1560 Neighboring gene uncharacterized LOC124905183 Neighboring gene H3K27ac hESC enhancer GRCh37_chrX:47372169-47372670 Neighboring gene H3K27ac hESC enhancer GRCh37_chrX:47372671-47373170 Neighboring gene MPRA-validated peak7378 silencer Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20805 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20806 Neighboring gene NUS1 pseudogene 1 Neighboring gene Sharpr-MPRA regulatory region 9461 Neighboring gene uncharacterized LOC124905184 Neighboring gene H3K27ac hESC enhancer GRCh37_chrX:47415445-47415946 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20807 Neighboring gene Sharpr-MPRA regulatory region 4668 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chrX:47434199-47434706 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chrX:47434707-47435213 Neighboring gene H3K4me1 hESC enhancer GRCh37_chrX:47435306-47435806 Neighboring gene Sharpr-MPRA regulatory region 5152 Neighboring gene CRISPRi-FlowFISH-validated HDAC6 regulatory element 2 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20808 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20809 Neighboring gene Sharpr-MPRA regulatory region 5754 Neighboring gene synapsin I Neighboring gene TIMP metallopeptidase inhibitor 1 Neighboring gene microRNA 4769

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Pr55(Gag) gag The amplified luminescent proximity homogeneous assay (AlphaScreen) identifies the interaction of HIV-1 Gag with ARAF PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables 3-phosphoinositide-dependent protein kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables AMP-activated protein kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables ATP binding IEA
Inferred from Electronic Annotation
more info
 
enables DNA-dependent protein kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables MAP kinase kinase kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
 
enables Rho-dependent protein serine/threonine kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables eukaryotic translation initiation factor 2alpha kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables histone H2AS1 kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables histone H2AS121 kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables histone H2AT120 kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables histone H2AXS139 kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables histone H2BS14 kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables histone H2BS36 kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables histone H3S10 kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables histone H3S28 kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables histone H3S57 kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables histone H3T11 kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables histone H3T3 kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables histone H3T45 kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables histone H3T6 kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables histone H4S1 kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein kinase activity TAS
Traceable Author Statement
more info
PubMed 
enables protein serine kinase activity IEA
Inferred from Electronic Annotation
more info
 
enables protein serine/threonine kinase activity IDA
Inferred from Direct Assay
more info
PubMed 
enables ribosomal protein S6 kinase activity IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in cytosol TAS
Traceable Author Statement
more info
 
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
 

General protein information

Preferred Names
serine/threonine-protein kinase A-Raf
Names
A-Raf proto-oncogene serine/threonine-protein kinase
Oncogene ARAF1
Ras-binding protein DA-Raf
proto-oncogene A-Raf-1
proto-oncogene Pks
v-raf murine sarcoma 3611 viral oncogene homolog 1
v-raf murine sarcoma 3611 viral oncogene-like protein
NP_001243125.1
NP_001243126.1
NP_001645.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_016339.2 RefSeqGene

    Range
    5089..15792
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001256196.2NP_001243125.1  serine/threonine-protein kinase A-Raf isoform 2

    See identical proteins and their annotated locations for NP_001243125.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) has an alternate splice site in the coding region, which does not affect the reading frame, compared to variant 1. The resulting isoform (2) is 3 aa longer than isoform 1.
    Source sequence(s)
    BC002466, BC007514, DC362427
    UniProtKB/TrEMBL
    A0A0S2Z3F2, A6NIT1, Q96II5
    Conserved Domains (3) summary
    cd01816
    Location:2091
    Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases
    cd14150
    Location:312576
    STKc_A-Raf; Catalytic domain of the Serine/Threonine Kinase, A-Raf (Rapidly Accelerated Fibrosarcoma) kinase
    pfam00130
    Location:99146
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  2. NM_001256197.2NP_001243126.1  serine/threonine-protein kinase A-Raf isoform 3

    See identical proteins and their annotated locations for NP_001243126.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks several exons and its transcription extends past a splice site that is used in variant 1, resulting in an immediate translation termination and a novel 3' UTR compared to variant 1. The resulting isoform (3) has a shorter C-terminus, compared to isoform 1.
    Source sequence(s)
    AB158254, CA424869, DC362427
    Consensus CDS
    CCDS59164.1
    UniProtKB/TrEMBL
    Q59HC8
    Related
    ENSP00000366238.1, ENST00000377039.2
    Conserved Domains (2) summary
    cd01816
    Location:2091
    Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases
    pfam00130
    Location:99146
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  3. NM_001654.5NP_001645.1  serine/threonine-protein kinase A-Raf isoform 1

    See identical proteins and their annotated locations for NP_001645.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the predominant isoform (1).
    Source sequence(s)
    BC002466, DC362427
    Consensus CDS
    CCDS35232.1
    UniProtKB/Swiss-Prot
    P07557, P10398, Q5H9B2, Q5H9B3
    UniProtKB/TrEMBL
    A0A024R178, A0A0S2Z3F2
    Related
    ENSP00000366244.4, ENST00000377045.9
    Conserved Domains (3) summary
    cd14150
    Location:309573
    STKc_A-Raf; Catalytic domain of the Serine/Threonine Kinase, A-Raf (Rapidly Accelerated Fibrosarcoma) kinase
    cd17133
    Location:1991
    RBD_ARAF; Ras-binding domain (RBD) found in serine/threonine-protein kinase ARAF
    pfam00130
    Location:99144
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000023.11 Reference GRCh38.p14 Primary Assembly

    Range
    47561205..47571908
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060947.1 Alternate T2T-CHM13v2.0

    Range
    46971095..46981798
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)