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ARAF A-Raf proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]

Gene ID: 369, updated on 29-Mar-2020

Summary

Official Symbol
ARAFprovided by HGNC
Official Full Name
A-Raf proto-oncogene, serine/threonine kinaseprovided by HGNC
Primary source
HGNC:HGNC:646
See related
Ensembl:ENSG00000078061 MIM:311010
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
PKS2; A-RAF; ARAF1; RAFA1
Summary
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]
Expression
Ubiquitous expression in bone marrow (RPKM 16.5), spleen (RPKM 14.0) and 25 other tissues See more
Orthologs

Genomic context

See ARAF in Genome Data Viewer
Location:
Xp11.3
Exon count:
16
Annotation release Status Assembly Chr Location
109.20200228 current GRCh38.p13 (GCF_000001405.39) X NC_000023.11 (47561100..47571921)
105 previous assembly GRCh37.p13 (GCF_000001405.25) X NC_000023.10 (47420499..47431320)

Chromosome X - NC_000023.11Genomic Context describing neighboring genes Neighboring gene zinc finger protein 41 Neighboring gene long intergenic non-protein coding RNA 1560 Neighboring gene NUS1 pseudogene 1 Neighboring gene Sharpr-MPRA regulatory region 4668 Neighboring gene synapsin I Neighboring gene TIMP metallopeptidase inhibitor 1 Neighboring gene microRNA 4769

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Pr55(Gag) gag The amplified luminescent proximity homogeneous assay (AlphaScreen) identifies the interaction of HIV-1 Gag with ARAF PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
metal ion binding IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein kinase activity TAS
Traceable Author Statement
more info
PubMed 
protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
protein serine/threonine kinase activity IDA
Inferred from Direct Assay
more info
PubMed 
Component Evidence Code Pubs
cellular_component ND
No biological Data available
more info
 
cytosol IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
cytosol TAS
Traceable Author Statement
more info
 
mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 

General protein information

Preferred Names
serine/threonine-protein kinase A-Raf
Names
A-Raf proto-oncogene serine/threonine-protein kinase
Oncogene ARAF1
Ras-binding protein DA-Raf
proto-oncogene A-Raf-1
proto-oncogene Pks
v-raf murine sarcoma 3611 viral oncogene homolog 1
v-raf murine sarcoma 3611 viral oncogene-like protein
NP_001243125.1
NP_001243126.1
NP_001645.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_016339.2 RefSeqGene

    Range
    5089..15792
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001256196.1NP_001243125.1  serine/threonine-protein kinase A-Raf isoform 2

    See identical proteins and their annotated locations for NP_001243125.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) has an alternate splice site in the coding region, which does not affect the reading frame, compared to variant 1. The resulting isoform (2) is 3 aa longer than isoform 1.
    Source sequence(s)
    BC002466, BC007514, DC362427
    Consensus CDS
    CCDS75970.1
    UniProtKB/Swiss-Prot
    P10398
    UniProtKB/TrEMBL
    Q96II5
    Related
    ENSP00000290277.7, ENST00000290277.10
    Conserved Domains (3) summary
    cd01816
    Location:2091
    Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases
    cd14150
    Location:312576
    STKc_A-Raf; Catalytic domain of the Serine/Threonine Kinase, A-Raf (Rapidly Accelerated Fibrosarcoma) kinase
    pfam00130
    Location:99146
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  2. NM_001256197.1NP_001243126.1  serine/threonine-protein kinase A-Raf isoform 3

    See identical proteins and their annotated locations for NP_001243126.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks several exons and its transcription extends past a splice site that is used in variant 1, resulting in an immediate translation termination and a novel 3' UTR compared to variant 1. The resulting isoform (3) has a shorter C-terminus, compared to isoform 1.
    Source sequence(s)
    AB158254, CA424869, DC362427
    Consensus CDS
    CCDS59164.1
    UniProtKB/Swiss-Prot
    P10398
    Related
    ENSP00000366238.1, ENST00000377039.2
    Conserved Domains (2) summary
    cd01816
    Location:2091
    Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases
    pfam00130
    Location:99146
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  3. NM_001654.5NP_001645.1  serine/threonine-protein kinase A-Raf isoform 1

    See identical proteins and their annotated locations for NP_001645.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the predominant isoform (1).
    Source sequence(s)
    BC002466, DC362427
    Consensus CDS
    CCDS35232.1
    UniProtKB/Swiss-Prot
    P10398
    UniProtKB/TrEMBL
    A0A024R178
    Related
    ENSP00000366244.4, ENST00000377045.9
    Conserved Domains (3) summary
    cd01816
    Location:2091
    Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases
    cd14150
    Location:309573
    STKc_A-Raf; Catalytic domain of the Serine/Threonine Kinase, A-Raf (Rapidly Accelerated Fibrosarcoma) kinase
    pfam00130
    Location:99146
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000023.11 Reference GRCh38.p13 Primary Assembly

    Range
    47561100..47571921
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
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