Lis-1 Lissencephaly-1 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Lis-1provided by FlyBase
Official Full Name: Lissencephaly-1provided by FlyBase
Primary source: FLYBASE:FBgn0015754
Locus tag: Dmel_CG8440
See related: AllianceGenome:FB:FBgn0015754
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG8440; D-Lis1; Dlis-1; DLis-1; DLIS-1; dLis1; Dlis1; DLis1; DLIS1; Dmel\CG8440; DmLIS1; l(2)k13209; l(2R)W8; Lis; Lis 1; lis-1; LIS-1; lis1; Lis1; n(2)k11702
Summary: Enables dynein complex binding activity. Involved in several processes, including generation of neurons; oogenesis; and transport along microtubule. Located in several cellular components, including growth cone; microtubule cytoskeleton; and neuronal cell body. Part of dynactin complex and dynein complex. Colocalizes with kinetochore; kinetochore microtubule; and spindle pole centrosome. Is expressed in several structures, including anterior-posterior subdivision of organism; embryonic/larval midgut primordium; ganglia; germline cell; and gut section. Human ortholog(s) of this gene implicated in lissencephaly and lissencephaly 1. Orthologous to human PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: 52F4-52F5; 2-78 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (16178826..16185103)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (12066331..12072608)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

lowering levels of Lissencephaly-1 (Lis1), a dynein motor co-factor, augments axonal mitochondrial transport in ageing wing neurons.
Title: Reducing Lissencephaly-1 levels augments mitochondrial transport and has a protective effect in adult Drosophila neurons.
The data reveal a critical role for Lis1 within the mRNA localization machinery and suggest a model in which Lis1 facilitates motor complex association with cargos by promoting the interaction of dynein with dynactin.
Title: Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs.
A general role of Lis-1/NudE, Dynein and microtubules, in cell-on-cell migration, is reported.
Title: Microtubules and Lis-1/NudE/dynein regulate invasive cell-on-cell migration in Drosophila.
present a model in which Lis-1 and asun cooperate to regulate dynein localization and centrosome positioning during spermatogenesis
Title: Regulation of dynein localization and centrosome positioning by Lis-1 and asunder during Drosophila spermatogenesis.
Lis1, which is widely expressed in adult mammalian tissues, could regulate adult tissue stem cells through modulating niche signaling and adhesion.
Title: Lissencephaly-1 controls germline stem cell self-renewal through modulating bone morphogenetic protein signaling and niche adhesion.
Neuroglian regulates the localisation of the nucleokinesis complex protein lissencephaly-1.
Title: The Drosophila cell adhesion molecule Neuroglian regulates Lissencephaly-1 localisation in circulating immunosurveillance cells.
Mutants in the Lis1/dynactin complex strongly decrease maximum and average spindle velocity, consistent with this motor complex mediating spindle/cortex forces.
Title: Lis1/dynactin regulates metaphase spindle orientation in Drosophila neuroblasts.
We conclude that Lis1/dynactin act together to regulate multiple, independent functions in mitotic cells, including spindle formation and cell cycle checkpoint release.
Title: Live imaging of Drosophila brain neuroblasts reveals a role for Lis1/dynactin in spindle assembly and mitotic checkpoint control.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables dynein complex binding	IBA Inferred from Biological aspect of Ancestor more info
enables dynein complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables dynein complex binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables microtubule plus-end binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in axo-dendritic transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axonal transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in brain morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in centrosome localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centrosome separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chorion-containing eggshell pattern formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in clathrin-dependent endocytosis involved in vitellogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in dendrite development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle orientation	IBA Inferred from Biological aspect of Ancestor more info
involved_in female germ-line cyst formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fusome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ cell development	IBA Inferred from Biological aspect of Ancestor more info
involved_in germarium-derived cystoblast division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germarium-derived oocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germarium-derived oocyte fate determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in kinetochore organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule organizing center organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in muscle cell cellular homeostasis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in muscle cell cellular homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mushroom body development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuroblast proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in nuclear migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear migration along microtubule	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nucleus localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte fate determination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in oocyte nucleus migration involved in oocyte dorsal/ventral axis specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ovarian fusome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ovarian nurse cell to oocyte transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in photoreceptor cell axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of BMP signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitotic metaphase/anaphase transition	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of mitotic metaphase/anaphase transition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrograde axonal transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in retrograde axonal transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rhabdomere development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in stem cell population maintenance	IDA Inferred from Direct Assay more info	PubMed
involved_in transport along microtubule	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle transport along microtubule	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasmic microtubule	IBA Inferred from Biological aspect of Ancestor more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
part_of dynactin complex	IDA Inferred from Direct Assay more info	PubMed
part_of dynein complex	IDA Inferred from Direct Assay more info	PubMed
located_in growth cone	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with kinetochore microtubule	IDA Inferred from Direct Assay more info	PubMed
part_of microtubule associated complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
is_active_in neuronal cell body	IBA Inferred from Biological aspect of Ancestor more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
is_active_in nuclear envelope	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle pole	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with spindle pole centrosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Lissencephaly-1

Names: CG8440-PA; CG8440-PB; CG8440-PF; CG8440-PG; Lis-1-PA; Lis-1-PB; Lis-1-PF; Lis-1-PG; Paf-AHbeta; lissencephaly-1; platelet-activating factor acetylhydrolase Ib beta-subunit homolog

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

16178826..16185103

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057812.5 → NP_477160.1 Lissencephaly-1, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477160.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZJ3, O96698, Q5BI20, Q7KNS3, Q8SXJ6

UniProtKB/TrEMBL

A0A0B4K812

Related

FBpp0086375

Conserved Domains (3) summary

smart00667
Location:9 → 40

LisH; Lissencephaly type-1-like homology motif

cd00200
Location:100 → 409

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:111 → 148

7WD40; WD40 repeat [structural motif]
NM_001259432.2 → NP_001246361.1 Lissencephaly-1, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246361.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZJ3, O96698, Q5BI20, Q7KNS3, Q8SXJ6

UniProtKB/TrEMBL

A0A0B4K812

Conserved Domains (3) summary

smart00667
Location:9 → 40

LisH; Lissencephaly type-1-like homology motif

cd00200
Location:100 → 409

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:111 → 148

7WD40; WD40 repeat [structural motif]
NM_176190.2 → NP_788370.1 Lissencephaly-1, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_788370.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZJ3, O96698, Q5BI20, Q7KNS3, Q8SXJ6

UniProtKB/TrEMBL

A0A0B4K812

Related

FBpp0086379

Conserved Domains (3) summary

smart00667
Location:9 → 40

LisH; Lissencephaly type-1-like homology motif

cd00200
Location:100 → 409

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:111 → 148

7WD40; WD40 repeat [structural motif]
NM_206130.2 → NP_995852.1 Lissencephaly-1, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_995852.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZJ3, O96698, Q5BI20, Q7KNS3, Q8SXJ6

UniProtKB/TrEMBL

A0A0B4K812

Conserved Domains (3) summary

smart00667
Location:9 → 40

LisH; Lissencephaly type-1-like homology motif

cd00200
Location:100 → 409

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:111 → 148

7WD40; WD40 repeat [structural motif]