ush u-shaped [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: ushprovided by FlyBase
Official Full Name: u-shapedprovided by FlyBase
Primary source: FLYBASE:FBgn0003963
Locus tag: Dmel_CG2762
See related: AllianceGenome:FB:FBgn0003963
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG2762; dFOG; Dmel\CG2762; FOG; l(2)19; u-sh; Ush; USH; ushaped; ZFPM2
Summary: Enables DNA-binding transcription repressor activity and RNA polymerase II-specific DNA-binding transcription factor binding activity. Involved in several processes, including embryonic development via the syncytial blastoderm; larval lymph gland hemocyte differentiation; and regulation of gene expression. Located in nucleus. Is expressed in several structures, including embryonic/larval midgut primordium; epithelium; hemocyte; imaginal disc; and nephrocyte. Human ortholog(s) of this gene implicated in 46,XY sex reversal 9; congenital diaphragmatic hernia; myelodysplastic syndrome; and tetralogy of Fallot. Orthologous to several human genes including ZFPM1 (zinc finger protein, FOG family member 1). [provided by Alliance of Genome Resources, Apr 2025]
Orthologs: all
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Genomic context

Location:: 21D1-21E2; 2-1 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (476220..540560)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (476220..540560)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ush regulates hemocyte-specific gene expression, fatty acid metabolism and cell cycle progression and cooperates with dNuRD to orchestrate hematopoiesis.
Title: Ush regulates hemocyte-specific gene expression, fatty acid metabolism and cell cycle progression and cooperates with dNuRD to orchestrate hematopoiesis.
It has been shown that Dorsal repressed Ush expression levels to promote hemocytes differentiation, whereas Cactus maintained Ush levels to block differentiation.
Title: The Friend of GATA Transcriptional Co-Regulator, U-Shaped, Is a Downstream Antagonist of Dorsal-Driven Prohemocyte Differentiation in Drosophila.
This study uncovers complex transcriptional interactions between the pnr isoforms and the cofactor Ush that may be important for regulation of proneural expression and thorax patterning.
Title: Transcriptional interactions between the pannier isoforms and the cofactor U-shaped during neural development in Drosophila.
Mechanistic insights into hh transcriptional regulation in hematopoietic progenitor niche cells, demonstrate the requirement of the Srp, Su(H) and Ush proteins in the control of niche cell differentiation and blood cell precursor maintenance.
Title: Serpent, suppressor of hairless and U-shaped are crucial regulators of hedgehog niche expression and prohemocyte maintenance during Drosophila larval hematopoiesis.
a conserved microRNA miR-8 and its target, USH, regulate body size in Drosophila. miR-8 null flies are smaller in size and defective in insulin signaling in fat body that is the fly counterpart of liver and adipose tissue.
Title: Conserved MicroRNA miR-8/miR-200 and its target USH/FOG2 control growth by regulating PI3K.
The Friend of GATA protein U-shaped is an important regulator of lymph gland prohemocyte potency and differentiation.
Title: Upregulation of the Drosophila Friend of GATA gene U-shaped by JAK/STAT signaling maintains lymph gland prohemocyte potency.
Results demonstrate that U-shaped acts to control larval hemocyte proliferation and suppress lamellocyte differentiation, likely regulating hematopoietic events downstream of Hop kinase activity.
Title: The Friend of GATA protein U-shaped functions as a hematopoietic tumor suppressor in Drosophila.
Results show that the GATA factor Serpent cross-regulates lozenge and u-shaped expression during Drosophila blood cell development.
Title: The GATA factor Serpent cross-regulates lozenge and u-shaped expression during Drosophila blood cell development.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm0099 (e-PCR)
- UniSTS:46461

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA-binding transcription repressor activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in amnioserosa maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in chaeta development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in compound eye development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ-band shortening	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heart development	IBA Inferred from Biological aspect of Ancestor more info
involved_in heart development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in larval lymph gland hemopoiesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lymph gland crystal cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lymph gland development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lymph gland plasmatocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of hemocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of insulin receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of antibacterial peptide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: u-shaped

Names: CG2762-PA; CG2762-PB; CG2762-PC; CG2762-PD; CG2762-PE; U-shaped; drosophila friend of GATA; friend of GATA; ush-PA; ush-PB; ush-PC; ush-PD; ush-PE; ushaped

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

476220..540560

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001272902.1 → NP_001259831.1 u-shaped, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259831.1

Status: REVIEWED

UniProtKB/TrEMBL

C1C573, M9PC12

Conserved Domains (2) summary

sd00019
Location:891 → 911

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam13909
Location:990 → 1014

zf-H2C2_5; C2H2-type zinc-finger domain
NM_001272901.1 → NP_001259830.1 u-shaped, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259830.1

Status: REVIEWED

UniProtKB/TrEMBL

C1C573, M9PDU3

Conserved Domains (2) summary

sd00019
Location:868 → 888

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam13909
Location:967 → 991

zf-H2C2_5; C2H2-type zinc-finger domain
NM_001272899.1 → NP_001259828.1 u-shaped, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259828.1

Status: REVIEWED

UniProtKB/TrEMBL

C1C573, M9PC12

Conserved Domains (2) summary

sd00019
Location:891 → 911

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam13909
Location:990 → 1014

zf-H2C2_5; C2H2-type zinc-finger domain
NM_057432.3 → NP_476780.1 u-shaped, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476780.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O18414, Q6AWH0, Q9VPQ6

UniProtKB/TrEMBL

C1C573

Related

FBpp0077723

Conserved Domains (2) summary

sd00019
Location:884 → 904

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam13909
Location:983 → 1007

zf-H2C2_5; C2H2-type zinc-finger domain
NM_001272900.1 → NP_001259829.1 u-shaped, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259829.1

Status: REVIEWED

UniProtKB/TrEMBL

C1C573, M9PBN1

Conserved Domains (2) summary

sd00019
Location:884 → 904

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam13909
Location:983 → 1007

zf-H2C2_5; C2H2-type zinc-finger domain