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Zfp516 zinc finger protein 516 [ Mus musculus (house mouse) ]

Gene ID: 329003, updated on 26-Sep-2022

Summary

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Official Symbol
Zfp516provided by MGI
Official Full Name
zinc finger protein 516provided by MGI
Primary source
MGI:MGI:2443957
See related
Ensembl:ENSMUSG00000058881 AllianceGenome:MGI:2443957
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Zfp26l; Znf516; mKIAA0222; D230016L03; C330029B10Rik
Summary
Enables DNA-binding transcription factor binding activity and cis-regulatory region sequence-specific DNA binding activity. Involved in several processes, including adipose tissue development; brown fat cell differentiation; and positive regulation of cold-induced thermogenesis. Located in nucleus. Is expressed in several structures, including alimentary system; brain; genitourinary system; respiratory system; and sensory organ. Orthologous to human ZNF516 (zinc finger protein 516). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in ovary adult (RPKM 8.7), limb E14.5 (RPKM 5.0) and 27 other tissues See more
Orthologs
human all
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Genomic context

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See Zfp516 in Genome Data Viewer
Location:
18; 18 E3
Exon count:
13
Annotation release Status Assembly Chr Location
109 current GRCm39 (GCF_000001635.27) 18 NC_000084.7 (82925324..83023439)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 18 NC_000084.6 (82909688..83005314)

Chromosome 18 - NC_000084.7Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA 4930445N18 gene Neighboring gene RIKEN cDNA 4930592I03 gene Neighboring gene predicted gene, 30889 Neighboring gene predicted gene, 41795 Neighboring gene predicted gene 10523 Neighboring gene RIKEN cDNA 1700095A13 gene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Zinc finger proteins orchestrate active gene silencing during embryonic stem cell differentiation. Kwak S, et al. Nucleic Acids Res, 2018 Jul 27. PMID 29846698, Free PMC Article
  2. LSD1 Interacts with Zfp516 to Promote UCP1 Transcription and Brown Fat Program. Sambeat A, et al. Cell Rep, 2016 Jun 14. PMID 27264172, Free PMC Article
  3. Cold-inducible Zfp516 activates UCP1 transcription to promote browning of white fat and development of brown fat. Dempersmier J, et al. Mol Cell, 2015 Jan 22. PMID 25578880, Free PMC Article
  4. PWWP2B Fine-Tunes Adipose Thermogenesis by Stabilizing HDACs in a NuRD Subcomplex. Yan L, et al. Adv Sci (Weinh), 2021 Aug. PMID 34180153, Free PMC Article
  5. Transcriptional Networks Controlled by NKX2-1 in the Development of Forebrain GABAergic Neurons. Sandberg M, et al. Neuron, 2016 Sep 21. PMID 27657450, Free PMC Article

See all (31) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Zfp217 and Zfp516 facilitate Ctbp2-mediated repression on active embryonic stem cell genes during differentiation.
    Title: Zinc finger proteins orchestrate active gene silencing during embryonic stem cell differentiation.
  2. through interaction with Zfp516, LSD1 is recruited to UCP1 and other brown adipose tissue-enriched genes.
    Title: LSD1 Interacts with Zfp516 to Promote UCP1 Transcription and Brown Fat Program.
  3. Zfp516 directly binds to the proximal region of the UCP1 promoter, not to the enhancer region where other transcription factors bind, and interacts with PRDM16 to activate the UCP1 promoter.
    Title: Cold-inducible Zfp516 activates UCP1 transcription to promote browning of white fat and development of brown fat.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (1) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
enables DNA-binding transcription factor binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
enables cis-regulatory region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in adipose tissue development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in brown fat cell differentiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of cold-induced thermogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of DNA-templated transcription IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
involved_in response to cold IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
zinc finger protein 516

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001177464.1 → NP_001170935.1  zinc finger protein 516

    See identical proteins and their annotated locations for NP_001170935.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.
    Source sequence(s)
    AC142100, BC053104, CJ101707
    Consensus CDS
    CCDS29380.1
    UniProtKB/Swiss-Prot
    Q7TSH3
    Related
    ENSMUSP00000071216.7, ENSMUST00000071233.7
    Conserved Domains (5) summary
    PHA03247
    Location:811 → 1036
    PHA03247; large tegument protein UL36; Provisional
    sd00017
    Location:36 → 56
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:34 → 56
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:48 → 73
    zf-H2C2_2; Zinc-finger double domain
    pfam13909
    Location:62 → 86
    zf-H2C2_5; C2H2-type zinc-finger domain

  2. NM_183033.2 → NP_898854.1  zinc finger protein 516

    See identical proteins and their annotated locations for NP_898854.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.
    Source sequence(s)
    AC142100, BC053104, CJ101868
    Consensus CDS
    CCDS29380.1
    UniProtKB/Swiss-Prot
    Q7TSH3
    Related
    ENSMUSP00000126629.2, ENSMUST00000171238.8
    Conserved Domains (5) summary
    PHA03247
    Location:811 → 1036
    PHA03247; large tegument protein UL36; Provisional
    sd00017
    Location:36 → 56
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:34 → 56
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:48 → 73
    zf-H2C2_2; Zinc-finger double domain
    pfam13909
    Location:62 → 86
    zf-H2C2_5; C2H2-type zinc-finger domain

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000084.7 Reference GRCm39 C57BL/6J

    Range
    82925324..83023439
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006526504.5 → XP_006526567.1  zinc finger protein 516 isoform X2

    Conserved Domains (4) summary
    PHA03247
    Location:811 → 1036
    PHA03247; large tegument protein UL36; Provisional
    sd00017
    Location:36 → 56
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:34 → 56
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:48 → 73
    zf-H2C2_2; Zinc-finger double domain

  2. XM_036161187.1 → XP_036017080.1  zinc finger protein 516 isoform X3

    Conserved Domains (3) summary
    sd00017
    Location:36 → 56
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:34 → 56
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:48 → 73
    zf-H2C2_2; Zinc-finger double domain

  3. XM_036161188.1 → XP_036017081.1  zinc finger protein 516 isoform X3

    Conserved Domains (3) summary
    sd00017
    Location:36 → 56
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:34 → 56
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:48 → 73
    zf-H2C2_2; Zinc-finger double domain

  4. XM_030250516.2 → XP_030106376.1  zinc finger protein 516 isoform X2

    Conserved Domains (4) summary
    PHA03247
    Location:811 → 1036
    PHA03247; large tegument protein UL36; Provisional
    sd00017
    Location:36 → 56
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:34 → 56
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:48 → 73
    zf-H2C2_2; Zinc-finger double domain

  5. XM_006526502.4 → XP_006526565.1  zinc finger protein 516 isoform X1

    See identical proteins and their annotated locations for XP_006526565.1

    UniProtKB/Swiss-Prot
    Q7TSH3
    Conserved Domains (5) summary
    PHA03247
    Location:811 → 1036
    PHA03247; large tegument protein UL36; Provisional
    sd00017
    Location:36 → 56
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:34 → 56
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:48 → 73
    zf-H2C2_2; Zinc-finger double domain
    pfam13909
    Location:62 → 86
    zf-H2C2_5; C2H2-type zinc-finger domain

  6. XM_006526500.4 → XP_006526563.1  zinc finger protein 516 isoform X1

    See identical proteins and their annotated locations for XP_006526563.1

    UniProtKB/Swiss-Prot
    Q7TSH3
    Conserved Domains (5) summary
    PHA03247
    Location:811 → 1036
    PHA03247; large tegument protein UL36; Provisional
    sd00017
    Location:36 → 56
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:34 → 56
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:48 → 73
    zf-H2C2_2; Zinc-finger double domain
    pfam13909
    Location:62 → 86
    zf-H2C2_5; C2H2-type zinc-finger domain

  7. XM_036161186.1 → XP_036017079.1  zinc finger protein 516 isoform X2

    Conserved Domains (4) summary
    PHA03247
    Location:811 → 1036
    PHA03247; large tegument protein UL36; Provisional
    sd00017
    Location:36 → 56
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:34 → 56
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:48 → 73
    zf-H2C2_2; Zinc-finger double domain

  8. XM_006526503.4 → XP_006526566.1  zinc finger protein 516 isoform X1

    See identical proteins and their annotated locations for XP_006526566.1

    UniProtKB/Swiss-Prot
    Q7TSH3
    Conserved Domains (5) summary
    PHA03247
    Location:811 → 1036
    PHA03247; large tegument protein UL36; Provisional
    sd00017
    Location:36 → 56
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:34 → 56
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:48 → 73
    zf-H2C2_2; Zinc-finger double domain
    pfam13909
    Location:62 → 86
    zf-H2C2_5; C2H2-type zinc-finger domain

  9. XM_006526501.5 → XP_006526564.1  zinc finger protein 516 isoform X1

    See identical proteins and their annotated locations for XP_006526564.1

    UniProtKB/Swiss-Prot
    Q7TSH3
    Conserved Domains (5) summary
    PHA03247
    Location:811 → 1036
    PHA03247; large tegument protein UL36; Provisional
    sd00017
    Location:36 → 56
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:34 → 56
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:48 → 73
    zf-H2C2_2; Zinc-finger double domain
    pfam13909
    Location:62 → 86
    zf-H2C2_5; C2H2-type zinc-finger domain

  10. XM_006526498.3 → XP_006526561.1  zinc finger protein 516 isoform X1

    See identical proteins and their annotated locations for XP_006526561.1

    UniProtKB/Swiss-Prot
    Q7TSH3
    Conserved Domains (5) summary
    PHA03247
    Location:811 → 1036
    PHA03247; large tegument protein UL36; Provisional
    sd00017
    Location:36 → 56
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:34 → 56
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:48 → 73
    zf-H2C2_2; Zinc-finger double domain
    pfam13909
    Location:62 → 86
    zf-H2C2_5; C2H2-type zinc-finger domain

  11. XM_006526499.5 → XP_006526562.1  zinc finger protein 516 isoform X1

    See identical proteins and their annotated locations for XP_006526562.1

    UniProtKB/Swiss-Prot
    Q7TSH3
    Conserved Domains (5) summary
    PHA03247
    Location:811 → 1036
    PHA03247; large tegument protein UL36; Provisional
    sd00017
    Location:36 → 56
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:34 → 56
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:48 → 73
    zf-H2C2_2; Zinc-finger double domain
    pfam13909
    Location:62 → 86
    zf-H2C2_5; C2H2-type zinc-finger domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q7TSH3.1 GenPept UniProtKB/Swiss-Prot:Q7TSH3

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