Marf Mitochondrial assembly regulatory factor [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Marfprovided by FlyBase
Official Full Name: Mitochondrial assembly regulatory factorprovided by FlyBase
Primary source: FLYBASE:FBgn0029870
Locus tag: Dmel_CG3869
See related: AllianceGenome:FB:FBgn0029870
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-WO0125274.3; CG3869; CG38869; dMarf; dMARF; Dmel\CG3869; dmfn; dMfn; dMFN; dMfn2; marf; MARF; Marf-1; mfn; Mfn; MFN; Mfn-2; mfn2; Mfn2; MFN2
Summary: Predicted to enable GTPase activity. Involved in several processes, including ecdysone biosynthetic process; mitochondrion organization; and regulation of mitochondrion organization. Located in mitochondrial outer membrane. Is expressed in several structures, including embryonic/larval gut; embryonic/larval midgut primordium; extended germ band embryo; germline cell; and pharyngeal muscle cell. Used to study Charcot-Marie-Tooth disease; Charcot-Marie-Tooth disease type 2; and Charcot-Marie-Tooth disease type 2A2A. Human ortholog(s) of this gene implicated in Charcot-Marie-Tooth disease; Charcot-Marie-Tooth disease type 2A2A; Charcot-Marie-Tooth disease type 2A2B; and Charcot-Marie-Tooth disease type 6. Orthologous to several human genes including MFN2 (mitofusin 2). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 5F4-5F4; 1-16 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (6365949..6370924)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (6259982..6264957)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A Novel Interaction between MFN2/Marf and MARK4/PAR-1 Is Implicated in Synaptic Defects and Mitochondrial Dysfunction.
Title: A Novel Interaction between MFN2/Marf and MARK4/PAR-1 Is Implicated in Synaptic Defects and Mitochondrial Dysfunction.
Marf-mediated mitochondrial fusion is imperative for the development and functioning of indirect flight muscles (IFMs) in drosophila.
Title: Marf-mediated mitochondrial fusion is imperative for the development and functioning of indirect flight muscles (IFMs) in drosophila.
Depletion of Mitofusin (dMfn) or Opa1 led to dysfunctional mitochondria, activation of Target of rapamycin (TOR) and a marked accumulation of lipid droplets. Enhancement of lipid utilization by the mitochondria attenuated TOR activation and rescued the loss of GSCs that was caused by inhibition of mitochondrial fusion.
Title: Mitochondrial fusion regulates lipid homeostasis and stem cell maintenance in the Drosophila testis.
both dominant negative and dominant active forms of mitofusin can cause Charcot-Marie-Tooth disease type 2A-associated defects.
Title: Mitofusin gain and loss of function drive pathogenesis in Drosophila models of CMT2A neuropathy.
binds cyclin A mRNA and shortens its poly-A tail to reduce Cyclin A protein level during oocyte maturation
Title: LOTUS domain protein MARF1 binds CCR4-NOT deadenylase complex to post-transcriptionally regulate gene expression in oocytes.
Enhancing the profusion gene mitofusin/marf is beneficial in an in vivo model of TDP-43 proteinopathies, serving as a potential therapeutic target.
Title: Enhancing Mitofusin/Marf ameliorates neuromuscular dysfunction in Drosophila models of TDP-43 proteinopathies.
activation of endoplasmic reticulum stress by defective mitochondria is neurotoxic in pink1 and parkin flies and that the reduction of this signalling is neuroprotective, independently of defective mitochondria.
Title: Mitofusin-mediated ER stress triggers neurodegeneration in pink1/parkin models of Parkinson's disease.
Clu is upstream of and binds to VCP in vivo and promotes VCP-dependent Marf degradation in vitro Marf accumulates in whole muscle lysates of clu-deficient flies and is destabilized upon Clu overexpression. Thus, Clu is essential for mitochondrial homeostasis and functions in concert with Parkin and VCP for Marf degradation to promote damaged mitochondrial clearance.
Title: Drosophila clueless is involved in Parkin-dependent mitophagy by promoting VCP-mediated Marf degradation.
lack of ChChd3 leads to inactivation of Hippo activity under normal development, which is also dependent on the transcriptional coactivator Yorkie (Yki). Furthermore, loss of ChChd3 induces oxidative stress and activates the JNK pathway. In addition, depletion of other mitochondrial fusion components, Opa1 or Marf, inactivates the Hippo pathway as well.
Title: Cross-Talk Between Mitochondrial Fusion and the Hippo Pathway in Controlling Cell Proliferation During Drosophila Development.
Marf is required for mitochondrial fusion and transport in long axons.
Title: Mitochondrial fusion but not fission regulates larval growth and synaptic development through steroid hormone production.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables GTPase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
involved_in adult locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axonal transport of mitochondrion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ecdysone biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endoplasmic reticulum organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid droplet organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_negative_effect lymph gland crystal cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_negative_effect lymph gland crystal cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial fusion	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrial fusion	IEA Inferred from Electronic Annotation more info
involved_in mitochondrial fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial fusion	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in mitochondrion localization	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrion organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitochondrial fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitochondrial fission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to endoplasmic reticulum stress	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrial envelope	ISS Inferred from Sequence or Structural Similarity more info	PubMed
is_active_in mitochondrial outer membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: mitochondrial assembly regulatory factor

Names: CG3869-PB; CG3869-PC; CG3869-PD; Marf-PB; Marf-PC; Marf-PD; drosophila mitofusin; mitochondria assembly regulatory factor; mitochondrial assembly; mitofusin; mitofusin/MARF; regulatory factor

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004354.4 Reference assembly

Range

6365949..6370924

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_132092.3 → NP_572320.2 mitochondrial assembly regulatory factor, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_572320.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q7YU24

Conserved Domains (3) summary

cd09912
Location:138 → 393

DLP_2; Dynamin-like protein including dynamins, mitofusins, and guanylate-binding proteins

pfam04799
Location:652 → 811

Fzo_mitofusin; fzo-like conserved region

cl21455
Location:92 → 172

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_206634.2 → NP_996357.1 mitochondrial assembly regulatory factor, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_996357.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0KHV7, Q7YU24, Q86DQ3, Q8T3J4, Q9W3Z9

Conserved Domains (3) summary

cd09912
Location:138 → 389

DLP_2; Dynamin-like protein including dynamins, mitofusins, and guanylate-binding proteins

pfam04799
Location:648 → 807

Fzo_mitofusin; fzo-like conserved region

cl21455
Location:92 → 172

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_206635.3 → NP_996358.2 mitochondrial assembly regulatory factor, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_996358.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0KHV7, Q7YU24, Q86DQ3, Q8T3J4, Q9W3Z9

Conserved Domains (3) summary

cd09912
Location:138 → 389

DLP_2; Dynamin-like protein including dynamins, mitofusins, and guanylate-binding proteins

pfam04799
Location:648 → 807

Fzo_mitofusin; fzo-like conserved region

cl21455
Location:92 → 172

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases