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Eps15 epidermal growth factor receptor pathway substrate 15 [ Rattus norvegicus (Norway rat) ]

Gene ID: 313474, updated on 15-May-2025
Official Symbol
Eps15provided by RGD
Official Full Name
epidermal growth factor receptor pathway substrate 15provided by RGD
Primary source
RGD:1305550
See related
Ensembl:ENSRNOG00000010299 AllianceGenome:RGD:1305550
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
RGD1307641
Summary
Predicted to enable identical protein binding activity; polyubiquitin modification-dependent protein binding activity; and ubiquitin binding activity. Involved in several processes, including endocytic recycling; positive regulation of receptor recycling; and postsynaptic neurotransmitter receptor internalization. Located in apical plasma membrane; basal plasma membrane; and early endosome. Is active in glutamatergic synapse and postsynaptic endocytic zone. Orthologous to human EPS15 (epidermal growth factor receptor pathway substrate 15). [provided by Alliance of Genome Resources, May 2025]
Expression
Biased expression in Spleen (RPKM 300.5), Brain (RPKM 275.3) and 9 other tissues See more
Orthologs
human mouse all
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See Eps15 in Genome Data Viewer
Location:
5q34-q35
Exon count:
25
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 5 NC_086023.1 (129274487..129374856)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 5 NC_051340.1 (124045865..124146221)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 5 NC_005104.4 (128923809..129022268)

Chromosome 5 - NC_086023.1Genomic Context describing neighboring genes Neighboring gene oxysterol binding protein-like 9 Neighboring gene uncharacterized LOC134486963 Neighboring gene uncharacterized LOC108351012 Neighboring gene calreticulin 4 Neighboring gene tetratricopeptide repeat domain 39A Neighboring gene ring finger protein 11 Neighboring gene RNA, U4 small nuclear 2

Go to nucleotide: Graphics FASTA GenBank

  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Related articles in PubMed

  1. Endocytic adaptor epidermal growth factor receptor substrate 15 (Eps15) is involved in the trafficking of ubiquitinated α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors. Lin A, et al. J Biol Chem, 2014 Aug 29. PMID 25023288, Free PMC Article
  2. Clathrin and clathrin-accessory proteins in rat kidney cortex epithelia. Hasse S, et al. Histochem Cell Biol, 2006 Aug. PMID 16625367
  3. Eps15 homology domain-NPF motif interactions regulate clathrin coat assembly during synaptic vesicle recycling. Morgan JR, et al. J Biol Chem, 2003 Aug 29. PMID 12807910
  4. Binding to DPF-motif by the POB1 EH domain is responsible for POB1-Eps15 interaction. Santonico E, et al. BMC Biochem, 2007 Dec 21. PMID 18154663, Free PMC Article
  5. Eps15 is constitutively oligomerized due to homophilic interaction of its coiled-coil region. Tebar F, et al. J Biol Chem, 1997 Jun 13. PMID 9182572

See all (37) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Knockdown of Eps15 suppresses the internalization of GluA1 but not the mutant GluA1 that lacks ubiquitination sites, indicating a role of Eps15 for the internalization of ubiquitinated AMPARs
    Title: Endocytic adaptor epidermal growth factor receptor substrate 15 (Eps15) is involved in the trafficking of ubiquitinated α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors.
  2. distinct forms of Eps15 direct EGFR to either the late endosome/lysosome for degradation (Eps15) or to the recycling endosome for transit back to the cell surface (Eps15S)
    Title: Recycling of the epidermal growth factor receptor is mediated by a novel form of the clathrin adaptor protein Eps15.
  3. FCHO2 regulates clathrin-mediated endocytosis through its interactions with membranes and Eps15.
    Title: Characterization of the EFC/F-BAR domain protein, FCHO2.
  4. Nedd4-mediated ubiquitination of Cx43 is required to recruit Eps15, through its ubiquitin-interacting motif, and targets ubiquitinated Cx43 to the endocytic pathway.
    Title: Eps15 interacts with ubiquitinated Cx43 and mediates its internalization.
  5. Eps15 plays an important role in clathrin-coated vesicle formation not only at the plasma membrane but also at the TGN during the secretory process
    Title: Eps15 mediates vesicle trafficking from the trans-Golgi network via an interaction with the clathrin adaptor AP-1.
  6. Binds Stonin2 at the second EH domain.
    Title: Structure of the Eps15-stonin2 complex provides a molecular explanation for EH-domain ligand specificity.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables calcium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables polyubiquitin modification-dependent protein binding ISO
Inferred from Sequence Orthology
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ubiquitin binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in Golgi to endosome transport ISO
Inferred from Sequence Orthology
more info
  
involved_in clathrin coat assembly ISO
Inferred from Sequence Orthology
more info
  
involved_in endocytic recycling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in endocytosis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in endosomal transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of receptor recycling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in postsynaptic neurotransmitter receptor internalization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in postsynaptic neurotransmitter receptor internalization IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in postsynaptic neurotransmitter receptor internalization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in receptor-mediated endocytosis of virus by host cell ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of cell population proliferation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within regulation of protein localization ISO
Inferred from Sequence Orthology
more info
  
involved_in symbiont entry into host cell ISO
Inferred from Sequence Orthology
more info
  
involved_in ubiquitin-dependent endocytosis ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
part_of AP-2 adaptor complex ISO
Inferred from Sequence Orthology
more info
  
located_in aggresome ISO
Inferred from Sequence Orthology
more info
  
located_in apical plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in basal plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in ciliary membrane ISO
Inferred from Sequence Orthology
more info
  
part_of clathrin coat of coated pit IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of clathrin coat of coated pit ISO
Inferred from Sequence Orthology
more info
  
located_in clathrin-coated pit ISO
Inferred from Sequence Orthology
more info
  
located_in clathrin-coated vesicle ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in early endosome IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in glutamatergic synapse IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in glutamatergic synapse IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in intracellular membrane-bounded organelle ISO
Inferred from Sequence Orthology
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
  
is_active_in postsynaptic endocytic zone IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in postsynaptic endocytic zone IMP
Inferred from Mutant Phenotype
more info
 PubMed 
is_active_in synapse IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
epidermal growth factor receptor substrate 15

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001365390.1 → NP_001352319.1  epidermal growth factor receptor substrate 15

    Status: VALIDATED

    Source sequence(s)
    AB262963, JAXUCZ010000005
    UniProtKB/TrEMBL
    A0A8I5YC99, A7BFV9
    Related
    ENSRNOP00000084473.1, ENSRNOT00000116370.2
    Conserved Domains (2) summary
    smart00027
    Location:121 → 215
    EH; Eps15 homology domain
    cl25732
    Location:333 → 504
    SMC_N; RecF/RecN/SMC N terminal domain

RNA

  1. NR_158458.2 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000005

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086023.1 Reference GRCr8

    Range
    129274487..129374856
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_063287709.1 → XP_063143779.1  epidermal growth factor receptor substrate 15 isoform X7

    UniProtKB/TrEMBL
    Q5JC29

  2. XM_039109961.2 → XP_038965889.1  epidermal growth factor receptor substrate 15 isoform X1

    UniProtKB/TrEMBL
    A0A8I5YC99
    Related
    ENSRNOP00000077237.2, ENSRNOT00000116078.2
    Conserved Domains (2) summary
    smart00027
    Location:121 → 215
    EH; Eps15 homology domain
    COG1196
    Location:369 → 540
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

  3. XM_039109962.2 → XP_038965890.1  epidermal growth factor receptor substrate 15 isoform X6

    UniProtKB/TrEMBL
    Q5JC29
    Conserved Domains (2) summary
    smart00027
    Location:121 → 215
    EH; Eps15 homology domain
    COG1196
    Location:369 → 540
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

  4. XM_063287710.1 → XP_063143780.1  epidermal growth factor receptor substrate 15 isoform X8

    UniProtKB/TrEMBL
    Q5JC29

  5. XM_063287708.1 → XP_063143778.1  epidermal growth factor receptor substrate 15 isoform X5

    UniProtKB/TrEMBL
    A0A8I5YC99
    Related
    ENSRNOP00000059209.4, ENSRNOT00000064145.4

  6. XM_063287706.1 → XP_063143776.1  epidermal growth factor receptor substrate 15 isoform X3

    UniProtKB/TrEMBL
    A0A8I5YC99

  7. XM_063287705.1 → XP_063143775.1  epidermal growth factor receptor substrate 15 isoform X2

    UniProtKB/TrEMBL
    E9PSY8

  8. XM_063287707.1 → XP_063143777.1  epidermal growth factor receptor substrate 15 isoform X4

    UniProtKB/TrEMBL
    E9PSY8
    Related
    ENSRNOP00000089899.2, ENSRNOT00000098058.2
Nucleotide Protein Strain
Heading Accession and Version

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